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Protein

Na(+)/H(+) antiporter NhaA

Gene

nhaA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Na+/H+ antiporter that extrudes sodium in exchange for external protons. Catalyzes the exchange of 2 H+ per Na+. Can mediate sodium uptake when a transmembrane pH gradient is applied. Active at alkaline pH. Activity is strongly down-regulated below pH 6.5.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei133Essential for antiport activity1
Sitei163 – 164Essential for antiport activity2
Sitei163Essential for antiport activity1
Sitei241Important for pH sensorCurated1
Sitei249Important for pH sensorCurated1
Sitei252Important for pH sensorCurated1

GO - Molecular functioni

GO - Biological processi

  • proton transport Source: EcoCyc
  • regulation of pH Source: InterPro
  • sodium ion transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Antiport, Ion transport, Sodium transport, Transport

Keywords - Ligandi

Sodium

Enzyme and pathway databases

BioCyciEcoCyc:NHAA-MONOMER.
ECOL316407:JW0018-MONOMER.
MetaCyc:NHAA-MONOMER.

Protein family/group databases

TCDBi2.A.33.1.1. the nhaa na(+):h(+) antiporter (nhaa) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Na(+)/H(+) antiporter NhaA
Alternative name(s):
Sodium/proton antiporter NhaA
Gene namesi
Name:nhaA
Synonyms:ant
Ordered Locus Names:b0019, JW0018
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10652. nhaA.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 11Cytoplasmic1 PublicationAdd BLAST11
Transmembranei12 – 30Helical; Name=1Add BLAST19
Topological domaini31 – 58Periplasmic1 PublicationAdd BLAST28
Transmembranei59 – 85Helical; Name=2Add BLAST27
Topological domaini86 – 94Cytoplasmic1 Publication9
Transmembranei95 – 116Helical; Name=3Add BLAST22
Topological domaini117 – 120Periplasmic1 Publication4
Transmembranei121 – 143Helical; Name=4Add BLAST23
Topological domaini144 – 149Cytoplasmic1 Publication6
Transmembranei150 – 175Helical; Name=5Add BLAST26
Topological domaini176 – 181Periplasmic1 Publication6
Transmembranei182 – 200Helical; Name=6Add BLAST19
Topological domaini201 – 204Cytoplasmic1 Publication4
Transmembranei205 – 218Helical; Name=7Add BLAST14
Topological domaini219 – 222Periplasmic1 Publication4
Transmembranei223 – 236Helical; Name=8Add BLAST14
Topological domaini237 – 246Cytoplasmic1 Publication10
Transmembranei247 – 271Helical; Name=9Add BLAST25
Topological domaini272 – 289Periplasmic1 PublicationAdd BLAST18
Transmembranei290 – 311Helical; Name=10Add BLAST22
Topological domaini312 – 326Cytoplasmic1 PublicationAdd BLAST15
Transmembranei327 – 350Helical; Name=11Add BLAST24
Topological domaini351 – 356Periplasmic1 Publication6
Transmembranei357 – 382Helical; Name=12Add BLAST26
Topological domaini383 – 388Cytoplasmic1 Publication6

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi133D → N: Loss of antiport activity. Abolishes ability to grow on high salt medium. 1 Publication1
Mutagenesisi163D → C: Loss of antiport activity. 2 Publications1
Mutagenesisi163D → N: Loss of antiport activity. Abolishes ability to grow on high salt medium. 2 Publications1
Mutagenesisi164D → N: Loss of antiport activity. Abolishes ability to grow on high salt medium. 1 Publication1
Mutagenesisi225H → A: Loss of antiport activity. Abolishes ability to grow at alkaline pH. 2 Publications1
Mutagenesisi225H → C or S: Slightly reduced antiport activity. No effect on pH sensitivity. 2 Publications1
Mutagenesisi225H → D or R: Shifts threshold for pH-sensitive inactivation. 2 Publications1
Mutagenesisi338G → S: Loss of pH-sensitivity. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL5478.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000524101 – 388Na(+)/H(+) antiporter NhaAAdd BLAST388

Proteomic databases

PaxDbiP13738.
PRIDEiP13738.

Expressioni

Inductioni

Transcription stimulated by high Na+ concentrations.

Interactioni

Subunit structurei

Monomer and homodimer.2 Publications

Protein-protein interaction databases

BioGridi4259720. 28 interactors.
DIPiDIP-10335N.
STRINGi511145.b0019.

Chemistry databases

BindingDBiP13738.

Structurei

Secondary structure

1388
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi13 – 30Combined sources18
Beta strandi32 – 34Combined sources3
Helixi35 – 41Combined sources7
Beta strandi45 – 50Combined sources6
Beta strandi53 – 58Combined sources6
Helixi59 – 84Combined sources26
Turni86 – 88Combined sources3
Turni91 – 93Combined sources3
Helixi95 – 104Combined sources10
Turni105 – 108Combined sources4
Helixi109 – 112Combined sources4
Helixi113 – 115Combined sources3
Helixi122 – 125Combined sources4
Beta strandi127 – 130Combined sources4
Helixi134 – 142Combined sources9
Beta strandi146 – 148Combined sources3
Beta strandi150 – 152Combined sources3
Helixi153 – 174Combined sources22
Helixi181 – 199Combined sources19
Helixi206 – 218Combined sources13
Turni219 – 221Combined sources3
Helixi223 – 236Combined sources14
Helixi241 – 243Combined sources3
Helixi247 – 261Combined sources15
Helixi263 – 271Combined sources9
Beta strandi277 – 279Combined sources3
Helixi284 – 287Combined sources4
Turni290 – 293Combined sources4
Helixi294 – 298Combined sources5
Helixi301 – 303Combined sources3
Turni305 – 311Combined sources7
Turni315 – 317Combined sources3
Helixi325 – 328Combined sources4
Helixi331 – 334Combined sources4
Helixi339 – 349Combined sources11
Turni351 – 353Combined sources3
Helixi358 – 370Combined sources13
Turni375 – 377Combined sources3
Helixi378 – 380Combined sources3
Turni381 – 383Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZCDX-ray3.45A/B1-388[»]
3FI1electron microscopy7.00A9-384[»]
4ATVX-ray3.50A/B/C/D1-388[»]
4AU5X-ray3.70A/B/C/D1-388[»]
ProteinModelPortaliP13738.
SMRiP13738.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP13738.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni45 – 58Important for dimerizationAdd BLAST14

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105DRC. Bacteria.
COG3004. LUCA.
HOGENOMiHOG000218489.
InParanoidiP13738.
KOiK03313.
OMAiQKAIFPA.
PhylomeDBiP13738.

Family and domain databases

Gene3Di1.20.1530.10. 1 hit.
HAMAPiMF_01844. NhaA. 1 hit.
InterProiIPR023171. Na/H_antiporter_dom.
IPR004670. NhaA.
[Graphical view]
PANTHERiPTHR30341:SF0. PTHR30341:SF0. 1 hit.
PfamiPF06965. Na_H_antiport_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00773. NhaA. 1 hit.

Sequencei

Sequence statusi: Complete.

P13738-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKHLHRFFSS DASGGIILII AAILAMIMAN SGATSGWYHD FLETPVQLRV
60 70 80 90 100
GSLEINKNML LWINDALMAV FFLLVGLEVK RELMQGSLAS LRQAAFPVIA
110 120 130 140 150
AIGGMIVPAL LYLAFNYADP ITREGWAIPA ATDIAFALGV LALLGSRVPL
160 170 180 190 200
ALKIFLMALA IIDDLGAIII IALFYTNDLS MASLGVAAVA IAVLAVLNLC
210 220 230 240 250
GARRTGVYIL VGVVLWTAVL KSGVHATLAG VIVGFFIPLK EKHGRSPAKR
260 270 280 290 300
LEHVLHPWVA YLILPLFAFA NAGVSLQGVT LDGLTSILPL GIIAGLLIGK
310 320 330 340 350
PLGISLFCWL ALRLKLAHLP EGTTYQQIMV VGILCGIGFT MSIFIASLAF
360 370 380
GSVDPELINW AKLGILVGSI SSAVIGYSWL RVRLRPSV
Length:388
Mass (Da):41,356
Last modified:February 1, 1996 - v3
Checksum:iB508B1D2E5EE9130
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03879 Genomic DNA. Translation: AAA23448.2.
U00096 Genomic DNA. Translation: AAC73130.1.
AP009048 Genomic DNA. Translation: BAB96592.1.
S67239 Genomic DNA. Translation: AAB20348.1.
PIRiC64722.
RefSeqiNP_414560.1. NC_000913.3.
WP_000681354.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73130; AAC73130; b0019.
BAB96592; BAB96592; BAB96592.
GeneIDi944758.
KEGGiecj:JW0018.
eco:b0019.
PATRICi32115129. VBIEscCol129921_0016.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03879 Genomic DNA. Translation: AAA23448.2.
U00096 Genomic DNA. Translation: AAC73130.1.
AP009048 Genomic DNA. Translation: BAB96592.1.
S67239 Genomic DNA. Translation: AAB20348.1.
PIRiC64722.
RefSeqiNP_414560.1. NC_000913.3.
WP_000681354.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZCDX-ray3.45A/B1-388[»]
3FI1electron microscopy7.00A9-384[»]
4ATVX-ray3.50A/B/C/D1-388[»]
4AU5X-ray3.70A/B/C/D1-388[»]
ProteinModelPortaliP13738.
SMRiP13738.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259720. 28 interactors.
DIPiDIP-10335N.
STRINGi511145.b0019.

Chemistry databases

BindingDBiP13738.
ChEMBLiCHEMBL5478.

Protein family/group databases

TCDBi2.A.33.1.1. the nhaa na(+):h(+) antiporter (nhaa) family.

Proteomic databases

PaxDbiP13738.
PRIDEiP13738.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73130; AAC73130; b0019.
BAB96592; BAB96592; BAB96592.
GeneIDi944758.
KEGGiecj:JW0018.
eco:b0019.
PATRICi32115129. VBIEscCol129921_0016.

Organism-specific databases

EchoBASEiEB0646.
EcoGeneiEG10652. nhaA.

Phylogenomic databases

eggNOGiENOG4105DRC. Bacteria.
COG3004. LUCA.
HOGENOMiHOG000218489.
InParanoidiP13738.
KOiK03313.
OMAiQKAIFPA.
PhylomeDBiP13738.

Enzyme and pathway databases

BioCyciEcoCyc:NHAA-MONOMER.
ECOL316407:JW0018-MONOMER.
MetaCyc:NHAA-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP13738.
PROiP13738.

Family and domain databases

Gene3Di1.20.1530.10. 1 hit.
HAMAPiMF_01844. NhaA. 1 hit.
InterProiIPR023171. Na/H_antiporter_dom.
IPR004670. NhaA.
[Graphical view]
PANTHERiPTHR30341:SF0. PTHR30341:SF0. 1 hit.
PfamiPF06965. Na_H_antiport_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00773. NhaA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNHAA_ECOLI
AccessioniPrimary (citable) accession number: P13738
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: February 1, 1996
Last modified: November 2, 2016
This is version 152 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.