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P13723

- HEXA1_DICDI

UniProt

P13723 - HEXA1_DICDI

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Protein
Beta-hexosaminidase subunit A1
Gene
hexa1, nagA, DDB_G0287033
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines. This enzyme plays a role during the slug stage of development in the maintenance of pseudoplasmodia of normal size.1 Publication

Catalytic activityi

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei308 – 3081Proton donor By similarity

GO - Molecular functioni

  1. beta-N-acetylhexosaminidase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

ReactomeiREACT_210507. Glycosphingolipid metabolism.
REACT_220518. Keratan sulfate degradation.
REACT_221941. CS/DS degradation.
REACT_226217. Hyaluronan uptake and degradation.

Protein family/group databases

CAZyiGH20. Glycoside Hydrolase Family 20.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-hexosaminidase subunit A1 (EC:3.2.1.52)
Alternative name(s):
Beta-N-acetylhexosaminidase subunit A1
N-acetyl-beta-glucosaminidase subunit A1
Gene namesi
Name:hexa1
Synonyms:nagA
ORF Names:DDB_G0287033
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 4, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0287033. nagA.

Subcellular locationi

Lysosome 1 Publication

GO - Cellular componenti

  1. lysosome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818 Reviewed prediction
Add
BLAST
Chaini19 – 532514Beta-hexosaminidase subunit A1
PRO_0000012010Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi72 – 721N-linked (GlcNAc...) Reviewed prediction
Glycosylationi79 – 791N-linked (GlcNAc...) Reviewed prediction
Glycosylationi350 – 3501N-linked (GlcNAc...) Reviewed prediction
Glycosylationi427 – 4271N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

The N-terminus is blocked.
N-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiP13723.

Interactioni

Subunit structurei

Dimer.1 Publication

Protein-protein interaction databases

STRINGi44689.DDB_0191256.

Structurei

3D structure databases

ProteinModelPortaliP13723.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3525.
KOiK12373.
OMAiSAVEWCK.
PhylomeDBiP13723.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
3.30.379.10. 1 hit.
InterProiIPR025705. Beta_hexosaminidase_sua/sub.
IPR029018. Chitobiase/Hex_dom_2-like.
IPR015883. Glyco_hydro_20_cat-core.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR029019. HEX_eukaryotic_N.
[Graphical view]
PfamiPF00728. Glyco_hydro_20. 1 hit.
PF14845. Glycohydro_20b2. 1 hit.
[Graphical view]
PIRSFiPIRSF001093. B-hxosamndse_ab_euk_. 1 hit.
PRINTSiPR00738. GLHYDRLASE20.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF55545. SSF55545. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13723-1 [UniParc]FASTAAdd to Basket

« Hide

MIKKIILFFA VLIAIVIGQQ PLNVVPYPQQ VSIGTCVIPV APGSILIESN    50
IESATFSVSM DRYTNLFFPF SNESEPSSNE SFLLSVTIYS DDETLQLGID 100
ESYSLSIEQG SYQLKATNIY GAMRGLETFK QLIVYNELEN SYSIVCVSIS 150
DSPRYPWRGF MVDSARHYIP KNMILHMIDS LGFSKFNTLH WHMVDAVAFP 200
VESTTYPDLT KGAFSPSATF SHDDIQEVVA YAKTYGIRVI PEFDIPGHAA 250
AWGIGYPELV ATCPDYAANV NNIPLDISNP ATFTFIQNLF TEIAPLFIDN 300
YFHTGGDELV TGCWLEDPAI ANWMTKMGFS TTDAFQYFEN NLDVTMKSIN 350
RTKITWNDPI DYGVQLNPET LVQVWSSGSD LQGIVNSGYK ALVSFAWYLD 400
KQNPDNNIHY EWQDTWQDFY AADPTNNIST NAENIIGGEA TMWAEQINQV 450
NWDVRVWPRA IGIAERLWSA QSVNSVSLAL PRIGHFTCDL SRRGIQSGPL 500
FPDYCPMQDD LVFTMKPNTK LSKSEIKLIL NK 532
Length:532
Mass (Da):59,788
Last modified:January 1, 1990 - v1
Checksum:iCE651DD79F456514
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J04065 mRNA. Translation: AAA33230.1.
AAFI02000096 Genomic DNA. Translation: EAL63881.1.
PIRiA30766.
RefSeqiXP_637398.1. XM_632306.1.

Genome annotation databases

EnsemblProtistsiDDB0191256; DDB0191256; DDB_G0287033.
GeneIDi8625929.
KEGGiddi:DDB_G0287033.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J04065 mRNA. Translation: AAA33230.1 .
AAFI02000096 Genomic DNA. Translation: EAL63881.1 .
PIRi A30766.
RefSeqi XP_637398.1. XM_632306.1.

3D structure databases

ProteinModelPortali P13723.
ModBasei Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0191256.

Protein family/group databases

CAZyi GH20. Glycoside Hydrolase Family 20.

Proteomic databases

PRIDEi P13723.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0191256 ; DDB0191256 ; DDB_G0287033 .
GeneIDi 8625929.
KEGGi ddi:DDB_G0287033.

Organism-specific databases

dictyBasei DDB_G0287033. nagA.

Phylogenomic databases

eggNOGi COG3525.
KOi K12373.
OMAi SAVEWCK.
PhylomeDBi P13723.

Enzyme and pathway databases

Reactomei REACT_210507. Glycosphingolipid metabolism.
REACT_220518. Keratan sulfate degradation.
REACT_221941. CS/DS degradation.
REACT_226217. Hyaluronan uptake and degradation.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
3.30.379.10. 1 hit.
InterProi IPR025705. Beta_hexosaminidase_sua/sub.
IPR029018. Chitobiase/Hex_dom_2-like.
IPR015883. Glyco_hydro_20_cat-core.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR029019. HEX_eukaryotic_N.
[Graphical view ]
Pfami PF00728. Glyco_hydro_20. 1 hit.
PF14845. Glycohydro_20b2. 1 hit.
[Graphical view ]
PIRSFi PIRSF001093. B-hxosamndse_ab_euk_. 1 hit.
PRINTSi PR00738. GLHYDRLASE20.
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF55545. SSF55545. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of the cDNA which encodes beta-N-acetylhexosaminidase A from Dictyostelium discoideum. Complete amino acid sequence and homology with the human enzyme."
    Graham T.R., Zassenhaus H.P., Kaplan A.
    J. Biol. Chem. 263:16823-16829(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 105-113; 260-276 AND 295-306, FUNCTION, PTM, GLYCOSYLATION, SUBCELLULAR LOCATION.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  3. "Genetic analysis of the gene for N-acetylglucosaminidase in Dictyostelium discoideum."
    Loomis W.F.
    Genetics 88:277-284(1978) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
    Strain: NC-4.

Entry informationi

Entry nameiHEXA1_DICDI
AccessioniPrimary (citable) accession number: P13723
Secondary accession number(s): Q54KX2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: September 3, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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