Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P13723

- HEXA1_DICDI

UniProt

P13723 - HEXA1_DICDI

Protein

Beta-hexosaminidase subunit A1

Gene

hexa1

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 Jan 1990)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines. This enzyme plays a role during the slug stage of development in the maintenance of pseudoplasmodia of normal size.1 Publication

    Catalytic activityi

    Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei308 – 3081Proton donorBy similarity

    GO - Molecular functioni

    1. beta-N-acetylhexosaminidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    ReactomeiREACT_210507. Glycosphingolipid metabolism.
    REACT_220518. Keratan sulfate degradation.
    REACT_221941. CS/DS degradation.
    REACT_226217. Hyaluronan uptake and degradation.

    Protein family/group databases

    CAZyiGH20. Glycoside Hydrolase Family 20.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-hexosaminidase subunit A1 (EC:3.2.1.52)
    Alternative name(s):
    Beta-N-acetylhexosaminidase subunit A1
    N-acetyl-beta-glucosaminidase subunit A1
    Gene namesi
    Name:hexa1
    Synonyms:nagA
    ORF Names:DDB_G0287033
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 4, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0287033. nagA.

    Subcellular locationi

    Lysosome 1 Publication

    GO - Cellular componenti

    1. lysosome Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Lysosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Sequence AnalysisAdd
    BLAST
    Chaini19 – 532514Beta-hexosaminidase subunit A1PRO_0000012010Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi72 – 721N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi79 – 791N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi350 – 3501N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi427 – 4271N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    The N-terminus is blocked.
    N-glycosylated.1 Publication

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiP13723.

    Interactioni

    Subunit structurei

    Dimer.1 Publication

    Protein-protein interaction databases

    STRINGi44689.DDB_0191256.

    Structurei

    3D structure databases

    ProteinModelPortaliP13723.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 20 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG3525.
    KOiK12373.
    OMAiSAVEWCK.
    PhylomeDBiP13723.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    3.30.379.10. 1 hit.
    InterProiIPR025705. Beta_hexosaminidase_sua/sub.
    IPR029018. Chitobiase/Hex_dom_2-like.
    IPR015883. Glyco_hydro_20_cat-core.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR029019. HEX_eukaryotic_N.
    [Graphical view]
    PfamiPF00728. Glyco_hydro_20. 1 hit.
    PF14845. Glycohydro_20b2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001093. B-hxosamndse_ab_euk_. 1 hit.
    PRINTSiPR00738. GLHYDRLASE20.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF55545. SSF55545. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P13723-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIKKIILFFA VLIAIVIGQQ PLNVVPYPQQ VSIGTCVIPV APGSILIESN    50
    IESATFSVSM DRYTNLFFPF SNESEPSSNE SFLLSVTIYS DDETLQLGID 100
    ESYSLSIEQG SYQLKATNIY GAMRGLETFK QLIVYNELEN SYSIVCVSIS 150
    DSPRYPWRGF MVDSARHYIP KNMILHMIDS LGFSKFNTLH WHMVDAVAFP 200
    VESTTYPDLT KGAFSPSATF SHDDIQEVVA YAKTYGIRVI PEFDIPGHAA 250
    AWGIGYPELV ATCPDYAANV NNIPLDISNP ATFTFIQNLF TEIAPLFIDN 300
    YFHTGGDELV TGCWLEDPAI ANWMTKMGFS TTDAFQYFEN NLDVTMKSIN 350
    RTKITWNDPI DYGVQLNPET LVQVWSSGSD LQGIVNSGYK ALVSFAWYLD 400
    KQNPDNNIHY EWQDTWQDFY AADPTNNIST NAENIIGGEA TMWAEQINQV 450
    NWDVRVWPRA IGIAERLWSA QSVNSVSLAL PRIGHFTCDL SRRGIQSGPL 500
    FPDYCPMQDD LVFTMKPNTK LSKSEIKLIL NK 532
    Length:532
    Mass (Da):59,788
    Last modified:January 1, 1990 - v1
    Checksum:iCE651DD79F456514
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04065 mRNA. Translation: AAA33230.1.
    AAFI02000096 Genomic DNA. Translation: EAL63881.1.
    PIRiA30766.
    RefSeqiXP_637398.1. XM_632306.1.

    Genome annotation databases

    EnsemblProtistsiDDB0191256; DDB0191256; DDB_G0287033.
    GeneIDi8625929.
    KEGGiddi:DDB_G0287033.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04065 mRNA. Translation: AAA33230.1 .
    AAFI02000096 Genomic DNA. Translation: EAL63881.1 .
    PIRi A30766.
    RefSeqi XP_637398.1. XM_632306.1.

    3D structure databases

    ProteinModelPortali P13723.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0191256.

    Protein family/group databases

    CAZyi GH20. Glycoside Hydrolase Family 20.

    Proteomic databases

    PRIDEi P13723.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0191256 ; DDB0191256 ; DDB_G0287033 .
    GeneIDi 8625929.
    KEGGi ddi:DDB_G0287033.

    Organism-specific databases

    dictyBasei DDB_G0287033. nagA.

    Phylogenomic databases

    eggNOGi COG3525.
    KOi K12373.
    OMAi SAVEWCK.
    PhylomeDBi P13723.

    Enzyme and pathway databases

    Reactomei REACT_210507. Glycosphingolipid metabolism.
    REACT_220518. Keratan sulfate degradation.
    REACT_221941. CS/DS degradation.
    REACT_226217. Hyaluronan uptake and degradation.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    3.30.379.10. 1 hit.
    InterProi IPR025705. Beta_hexosaminidase_sua/sub.
    IPR029018. Chitobiase/Hex_dom_2-like.
    IPR015883. Glyco_hydro_20_cat-core.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR029019. HEX_eukaryotic_N.
    [Graphical view ]
    Pfami PF00728. Glyco_hydro_20. 1 hit.
    PF14845. Glycohydro_20b2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001093. B-hxosamndse_ab_euk_. 1 hit.
    PRINTSi PR00738. GLHYDRLASE20.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF55545. SSF55545. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of the cDNA which encodes beta-N-acetylhexosaminidase A from Dictyostelium discoideum. Complete amino acid sequence and homology with the human enzyme."
      Graham T.R., Zassenhaus H.P., Kaplan A.
      J. Biol. Chem. 263:16823-16829(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 105-113; 260-276 AND 295-306, FUNCTION, PTM, GLYCOSYLATION, SUBCELLULAR LOCATION.
    2. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    3. "Genetic analysis of the gene for N-acetylglucosaminidase in Dictyostelium discoideum."
      Loomis W.F.
      Genetics 88:277-284(1978) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT.
      Strain: NC-4.

    Entry informationi

    Entry nameiHEXA1_DICDI
    AccessioniPrimary (citable) accession number: P13723
    Secondary accession number(s): Q54KX2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: January 1, 1990
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3