P13723 (HEXA1_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-hexosaminidase subunit A1
EC=3.2.1.52

Alternative name(s):
Beta-N-acetylhexosaminidase subunit A1
N-acetyl-beta-glucosaminidase subunit A1

Gene names

Name:	hexa1
Synonyms:	nagA
ORF Names:	DDB_G0287033

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	532 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines. This enzyme plays a role during the slug stage of development in the maintenance of pseudoplasmodia of normal size. Ref.1
Catalytic activity	Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.
Subunit structure	Dimer. Ref.3
Subcellular location	Lysosome Ref.1.
Post-translational modification	The N-terminus is blocked. N-glycosylated. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 20 family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	beta-N-acetylhexosaminidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 532	514	Beta-hexosaminidase subunit A1		PRO_0000012010
Sites
	Active site	308	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	72	1	N-linked (GlcNAc...) Potential
	Glycosylation	79	1	N-linked (GlcNAc...) Potential
	Glycosylation	350	1	N-linked (GlcNAc...) Potential
	Glycosylation	427	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P13723 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: CE651DD79F456514	FASTA	532	59,788
	10 20 30 40 50 60 MIKKIILFFA VLIAIVIGQQ PLNVVPYPQQ VSIGTCVIPV APGSILIESN IESATFSVSM 70 80 90 100 110 120 DRYTNLFFPF SNESEPSSNE SFLLSVTIYS DDETLQLGID ESYSLSIEQG SYQLKATNIY 130 140 150 160 170 180 GAMRGLETFK QLIVYNELEN SYSIVCVSIS DSPRYPWRGF MVDSARHYIP KNMILHMIDS 190 200 210 220 230 240 LGFSKFNTLH WHMVDAVAFP VESTTYPDLT KGAFSPSATF SHDDIQEVVA YAKTYGIRVI 250 260 270 280 290 300 PEFDIPGHAA AWGIGYPELV ATCPDYAANV NNIPLDISNP ATFTFIQNLF TEIAPLFIDN 310 320 330 340 350 360 YFHTGGDELV TGCWLEDPAI ANWMTKMGFS TTDAFQYFEN NLDVTMKSIN RTKITWNDPI 370 380 390 400 410 420 DYGVQLNPET LVQVWSSGSD LQGIVNSGYK ALVSFAWYLD KQNPDNNIHY EWQDTWQDFY 430 440 450 460 470 480 AADPTNNIST NAENIIGGEA TMWAEQINQV NWDVRVWPRA IGIAERLWSA QSVNSVSLAL 490 500 510 520 530 PRIGHFTCDL SRRGIQSGPL FPDYCPMQDD LVFTMKPNTK LSKSEIKLIL NK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of the cDNA which encodes beta-N-acetylhexosaminidase A from Dictyostelium discoideum. Complete amino acid sequence and homology with the human enzyme." Graham T.R., Zassenhaus H.P., Kaplan A. J. Biol. Chem. 263:16823-16829(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 105-113; 260-276 AND 295-306, FUNCTION, PTM, GLYCOSYLATION, SUBCELLULAR LOCATION.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"Genetic analysis of the gene for N-acetylglucosaminidase in Dictyostelium discoideum." Loomis W.F. Genetics 88:277-284(1978) [PubMed] [Europe PMC] [Abstract] Cited for: SUBUNIT. Strain: NC-4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J04065 mRNA. Translation: AAA33230.1. AAFI02000096 Genomic DNA. Translation: EAL63881.1.
PIR	A30766.
RefSeq	XP_637398.1. XM_632306.1.
3D structure databases
ProteinModelPortal	P13723.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDB_0191256.
Protein family/group databases
CAZy	GH20. Glycoside Hydrolase Family 20.
Proteomic databases
PRIDE	P13723.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0191256; DDB0191256; DDB_G0287033.
GeneID	8625929.
KEGG	ddi:DDB_G0287033.
Organism-specific databases
dictyBase	DDB_G0287033. nagA.
Phylogenomic databases
eggNOG	COG3525.
KO	K12373.
OMA	SARMADY.
ProtClustDB	CLSZ2430037.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR025705. Beta_hexosaminidase_sua/sub. IPR015883. Glyco_hydro_20_cat-core. IPR015882. Glyco_hydro_20b. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00728. Glyco_hydro_20. 1 hit. PF02838. Glyco_hydro_20b. 1 hit. [Graphical view]
PIRSF	PIRSF001093. B-hxosamndse_ab_euk_. 1 hit.
PRINTS	PR00738. GLHYDRLASE20.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HEXA1_DICDI

Accession

Primary (citable) accession number: P13723
Secondary accession number(s): Q54KX2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	May 1, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)