Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Beta-galactoside alpha-2,6-sialyltransferase 1

Gene

St6gal1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transfers sialic acid from CMP-sialic acid to galactose-containing acceptor substrates.1 Publication

Catalytic activityi

CMP-N-acetyl-beta-neuraminate + beta-D-galactosyl-R = CMP + N-acetyl-alpha-neuraminyl-(2->6)-beta-D-galactosyl-R.1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei186Substrate; via amide nitrogenBy similarity1
Binding sitei209SubstrateBy similarity1
Binding sitei230SubstrateBy similarity1
Binding sitei350Substrate; via carbonyl oxygenBy similarity1
Binding sitei351SubstrateBy similarity1
Binding sitei362SubstrateBy similarity1
Binding sitei366SubstrateBy similarity1
Binding sitei373SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • N-acetylneuraminate metabolic process Source: UniProtKB
  • negative regulation of chemotaxis Source: RGD
  • negative regulation of macrophage apoptotic process Source: RGD
  • oligosaccharide metabolic process Source: GO_Central
  • positive regulation of mononuclear cell proliferation Source: RGD
  • protein glycosylation Source: RGD
  • protein N-linked glycosylation via asparagine Source: UniProtKB
  • protein sialylation Source: RGD
  • regulation of substrate adhesion-dependent cell spreading Source: RGD
  • response to ethanol Source: RGD
  • sialylation Source: UniProtKB

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.99.1 5301
UniPathwayiUPA00378

Protein family/group databases

CAZyiGT29 Glycosyltransferase Family 29

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactoside alpha-2,6-sialyltransferase 1 (EC:2.4.99.11 Publication)
Short name:
Alpha 2,6-ST 1
Alternative name(s):
CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1
ST6Gal I
Short name:
ST6GalI
Sialyltransferase 1
Gene namesi
Name:St6gal1
Synonyms:Siat1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3676 St6gal1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 9Cytoplasmic9
Transmembranei10 – 26Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST17
Topological domaini27 – 403LumenalSequence analysisAdd BLAST377

Keywords - Cellular componenti

Golgi apparatus, Membrane, Secreted

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4276

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001492511 – 403Beta-galactoside alpha-2,6-sialyltransferase 1Add BLAST403

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi139 ↔ 403By similarity
Glycosylationi146N-linked (GlcNAc...) asparagine1
Glycosylationi158N-linked (GlcNAc...) asparagine1
Disulfide bondi181 ↔ 3321 Publication
Glycosylationi285N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi350 ↔ 361By similarity
Modified residuei366PhosphotyrosineBy similarity1

Post-translational modificationi

The soluble form derives from the membrane form by proteolytic processing.

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP13721
PRIDEiP13721

PTM databases

iPTMnetiP13721
PhosphoSitePlusiP13721

Expressioni

Tissue specificityi

Strongly expressed in liver, spleen, lung, kidney and submaxillary gland and weakly in heart and brain.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000002499

Chemistry databases

BindingDBiP13721

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4MPSX-ray2.40A/B95-403[»]
SMRiP13721
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni63 – 403Catalytic (soluble form)Add BLAST341
Regioni319 – 321Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the glycosyltransferase 29 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2692 Eukaryota
ENOG410XT8P LUCA
HOGENOMiHOG000013206
HOVERGENiHBG052853
InParanoidiP13721
KOiK00778
PhylomeDBiP13721

Family and domain databases

Gene3Di3.90.1480.201 hit
InterProiView protein in InterPro
IPR001675 Glyco_trans_29
IPR038578 GT29-like_sf
IPR012163 Sialyl_trans
PfamiView protein in Pfam
PF00777 Glyco_transf_29, 1 hit
PIRSFiPIRSF005557 Sialyl_trans, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform RLA (identifier: P13721-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIHTNLKKKF SLFILVFLLF AVICVWKKGS DYEALTLQAK EFQMPKSQEK
60 70 80 90 100
VAMGSASQVV FSNSKQDPKE DIPILSYHRV TAKVKPQPSF QVWDKDSTYS
110 120 130 140 150
KLNPRLLKIW RNYLNMNKYK VSYKGPGPGV KFSVEALRCH LRDHVNVSMI
160 170 180 190 200
EATDFPFNTT EWEGYLPKEN FRTKVGPWQR CAVVSSAGSL KNSQLGREID
210 220 230 240 250
NHDAVLRFNG APTDNFQQDV GSKTTIRLMN SQLVTTEKRF LKDSLYTEGI
260 270 280 290 300
LIVWDPSVYH ADIPKWYQKP DYNFFETYKS YRRLNPSQPF YILKPQMPWE
310 320 330 340 350
LWDIIQEISA DLIQPNPPSS GMLGIIIMMT LCDQVDIYEF LPSKRKTDVC
360 370 380 390 400
YYHQKFFDSA CTMGAYDPLL FEKNMVKHLN EGTDEDIYLF GKATLSGFRN

IRC
Length:403
Mass (Da):46,732
Last modified:January 1, 1990 - v1
Checksum:iF40D7EA6BE67EA9A
GO
Isoform RKA (identifier: P13721-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-232: MIHTNLKKKF...KTTIRLMNSQ → MRYLLFWYGL...LSLLLLVLGK

Show »
Length:214
Mass (Da):25,188
Checksum:i1C05C601E3464550
GO
Isoform RKB (identifier: P13721-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-232: MIHTNLKKKF...KTTIRLMNSQ → MRYLLFWYGLPHS

Show »
Length:184
Mass (Da):21,874
Checksum:iBC829880512B2F53
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0017821 – 232MIHTN…LMNSQ → MRYLLFWYGLPHSYSQCVCH WTPASGIFENEPLLSLLLLV LGK in isoform RKA. CuratedAdd BLAST232
Alternative sequenceiVSP_0017831 – 232MIHTN…LMNSQ → MRYLLFWYGLPHS in isoform RKB. CuratedAdd BLAST232

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18769 mRNA Translation: AAA41196.1
M73987 mRNA Translation: AAB06269.1
M83143 mRNA Translation: AAB07233.1
PIRiA28451
C42327
RefSeqiNP_001106815.1, NM_001113344.1
NP_671738.2, NM_147205.2
UniGeneiRn.54567

Genome annotation databases

GeneIDi25197
KEGGirno:25197
UCSCiRGD:3676 rat [P13721-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSIAT1_RAT
AccessioniPrimary (citable) accession number: P13721
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: March 28, 2018
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome