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Protein

Beta-galactoside alpha-2,6-sialyltransferase 1

Gene

St6gal1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transfers sialic acid from CMP-sialic acid to galactose-containing acceptor substrates.1 Publication

Catalytic activityi

CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine = CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine.1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei186 – 1861Substrate; via amide nitrogenBy similarity
Binding sitei209 – 2091SubstrateBy similarity
Binding sitei230 – 2301SubstrateBy similarity
Binding sitei350 – 3501Substrate; via carbonyl oxygenBy similarity
Binding sitei351 – 3511SubstrateBy similarity
Binding sitei362 – 3621SubstrateBy similarity
Binding sitei366 – 3661SubstrateBy similarity
Binding sitei373 – 3731SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

  • N-acetylneuraminate metabolic process Source: UniProtKB
  • negative regulation of chemotaxis Source: RGD
  • negative regulation of macrophage apoptotic process Source: RGD
  • oligosaccharide metabolic process Source: GO_Central
  • positive regulation of mononuclear cell proliferation Source: RGD
  • protein N-linked glycosylation via asparagine Source: UniProtKB
  • protein sialylation Source: RGD
  • regulation of substrate adhesion-dependent cell spreading Source: RGD
  • response to ethanol Source: RGD
  • sialylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.99.1. 5301.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactoside alpha-2,6-sialyltransferase 1 (EC:2.4.99.1)
Short name:
Alpha 2,6-ST 1
Alternative name(s):
CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1
ST6Gal I
Short name:
ST6GalI
Sialyltransferase 1
Gene namesi
Name:St6gal1
Synonyms:Siat1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3676. St6gal1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 99Cytoplasmic
Transmembranei10 – 2617Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini27 – 403377LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum Source: RGD
  • extracellular region Source: UniProtKB-SubCell
  • Golgi cisterna membrane Source: UniProtKB-SubCell
  • Golgi medial cisterna Source: RGD
  • Golgi trans cisterna Source: RGD
  • integral component of Golgi membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane, Secreted

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL4276.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 403403Beta-galactoside alpha-2,6-sialyltransferase 1PRO_0000149251Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi139 ↔ 403By similarity
Glycosylationi146 – 1461N-linked (GlcNAc...)
Glycosylationi158 – 1581N-linked (GlcNAc...)
Disulfide bondi181 ↔ 3321 Publication
Glycosylationi285 – 2851N-linked (GlcNAc...)Sequence analysis
Disulfide bondi350 ↔ 361By similarity
Modified residuei366 – 3661PhosphotyrosineBy similarity

Post-translational modificationi

The soluble form derives from the membrane form by proteolytic processing.

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP13721.
PRIDEiP13721.

PTM databases

iPTMnetiP13721.
PhosphoSiteiP13721.

Expressioni

Tissue specificityi

Strongly expressed in liver, spleen, lung, kidney and submaxillary gland and weakly in heart and brain.

Interactioni

Protein-protein interaction databases

MINTiMINT-4564553.
STRINGi10116.ENSRNOP00000002499.

Chemistry

BindingDBiP13721.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4MPSX-ray2.40A/B95-403[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni63 – 403341Catalytic (soluble form)Add
BLAST
Regioni319 – 3213Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 29 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2692. Eukaryota.
ENOG410XT8P. LUCA.
HOGENOMiHOG000013206.
HOVERGENiHBG052853.
InParanoidiP13721.
KOiK00778.
PhylomeDBiP13721.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform RLA (identifier: P13721-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIHTNLKKKF SLFILVFLLF AVICVWKKGS DYEALTLQAK EFQMPKSQEK
60 70 80 90 100
VAMGSASQVV FSNSKQDPKE DIPILSYHRV TAKVKPQPSF QVWDKDSTYS
110 120 130 140 150
KLNPRLLKIW RNYLNMNKYK VSYKGPGPGV KFSVEALRCH LRDHVNVSMI
160 170 180 190 200
EATDFPFNTT EWEGYLPKEN FRTKVGPWQR CAVVSSAGSL KNSQLGREID
210 220 230 240 250
NHDAVLRFNG APTDNFQQDV GSKTTIRLMN SQLVTTEKRF LKDSLYTEGI
260 270 280 290 300
LIVWDPSVYH ADIPKWYQKP DYNFFETYKS YRRLNPSQPF YILKPQMPWE
310 320 330 340 350
LWDIIQEISA DLIQPNPPSS GMLGIIIMMT LCDQVDIYEF LPSKRKTDVC
360 370 380 390 400
YYHQKFFDSA CTMGAYDPLL FEKNMVKHLN EGTDEDIYLF GKATLSGFRN

IRC
Length:403
Mass (Da):46,732
Last modified:January 1, 1990 - v1
Checksum:iF40D7EA6BE67EA9A
GO
Isoform RKA (identifier: P13721-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-232: MIHTNLKKKF...KTTIRLMNSQ → MRYLLFWYGL...LSLLLLVLGK

Show »
Length:214
Mass (Da):25,188
Checksum:i1C05C601E3464550
GO
Isoform RKB (identifier: P13721-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-232: MIHTNLKKKF...KTTIRLMNSQ → MRYLLFWYGLPHS

Show »
Length:184
Mass (Da):21,874
Checksum:iBC829880512B2F53
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 232232MIHTN…LMNSQ → MRYLLFWYGLPHSYSQCVCH WTPASGIFENEPLLSLLLLV LGK in isoform RKA. CuratedVSP_001782Add
BLAST
Alternative sequencei1 – 232232MIHTN…LMNSQ → MRYLLFWYGLPHS in isoform RKB. CuratedVSP_001783Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18769 mRNA. Translation: AAA41196.1.
M73987 mRNA. Translation: AAB06269.1.
M83143 mRNA. Translation: AAB07233.1.
PIRiA28451.
C42327.
RefSeqiNP_001106815.1. NM_001113344.1.
NP_671738.2. NM_147205.2.
UniGeneiRn.54567.

Genome annotation databases

GeneIDi25197.
KEGGirno:25197.
UCSCiRGD:3676. rat. [P13721-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18769 mRNA. Translation: AAA41196.1.
M73987 mRNA. Translation: AAB06269.1.
M83143 mRNA. Translation: AAB07233.1.
PIRiA28451.
C42327.
RefSeqiNP_001106815.1. NM_001113344.1.
NP_671738.2. NM_147205.2.
UniGeneiRn.54567.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4MPSX-ray2.40A/B95-403[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4564553.
STRINGi10116.ENSRNOP00000002499.

Chemistry

BindingDBiP13721.
ChEMBLiCHEMBL4276.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

PTM databases

iPTMnetiP13721.
PhosphoSiteiP13721.

Proteomic databases

PaxDbiP13721.
PRIDEiP13721.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi25197.
KEGGirno:25197.
UCSCiRGD:3676. rat. [P13721-1]

Organism-specific databases

CTDi6480.
RGDi3676. St6gal1.

Phylogenomic databases

eggNOGiKOG2692. Eukaryota.
ENOG410XT8P. LUCA.
HOGENOMiHOG000013206.
HOVERGENiHBG052853.
InParanoidiP13721.
KOiK00778.
PhylomeDBiP13721.

Enzyme and pathway databases

UniPathwayiUPA00378.
BRENDAi2.4.99.1. 5301.

Miscellaneous databases

PROiP13721.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSIAT1_RAT
AccessioniPrimary (citable) accession number: P13721
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: July 6, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.