Reviewed, UniProtKB/Swiss-Prot P13721 (SIAT1_RAT)

Names and origin

Protein names

Recommended name:
    Beta-galactoside alpha-2,6-sialyltransferase 1
    EC=2.4.99.1
Alternative name(s):
    CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1
      Short name=Alpha 2,6-ST
      Short name=Sialyltransferase 1
    ST6Gal I

Gene names

Name:	St6gal1
Synonyms:	Siat1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	403 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates.
Catalytic activity	CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine = CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein. Secreted. Note: Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid.
Tissue specificity	Strongly expressed in liver, spleen, lung, kidney and submaxillary gland and weakly in heart and brain.
Post-translational modification	The soluble form derives from the membrane form by proteolytic processing.
Sequence similarities	Belongs to the glycosyltransferase 29 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	protein amino acid glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell integral to Golgi membrane Inferred from electronic annotation. Source: InterPro
Molecular function	beta-galactoside alpha-2,6-sialyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform RLA (identifier: P13721-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform RKA (identifier: P13721-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-232: MIHTNLKKKF...KTTIRLMNSQ → MRYLLFWYGL...LSLLLLVLGK

Isoform RKB (identifier: P13721-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-232: MIHTNLKKKF...KTTIRLMNSQ → MRYLLFWYGLPHS

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 403	403	Beta-galactoside alpha-2,6-sialyltransferase 1		PRO_0000149251
Regions
	Topological domain	1 – 9	9	Cytoplasmic
	Transmembrane	10 – 26	17	Signal-anchor for type II membrane protein Potential
	Topological domain	27 – 403	377	Lumenal Potential
	Region	63 – 403	341	Catalytic (soluble form)
Amino acid modifications
	Glycosylation	146	1	N-linked (GlcNAc...)
	Glycosylation	158	1	N-linked (GlcNAc...)
	Glycosylation	285	1	N-linked (GlcNAc...) Potential
	Disulfide bond	181 ↔ 332		Ref.5
Natural variations
	Alternative sequence	1 – 232	232	MIHTN…LMNSQ → MRYLLFWYGLPHSYSQCVCH WTPASGIFENEPLLSLLLLV LGK in isoform RKA.		VSP_001782
	Alternative sequence	1 – 232	232	MIHTN…LMNSQ → MRYLLFWYGLPHS in isoform RKB.		VSP_001783

Sequences

	Sequence		Length	Mass (Da)
	Isoform RLA [UniParc]. Last modified January 1, 1990. Version 1. Checksum: F40D7EA6BE67EA9A	FASTA	403	46,732
	10 20 30 40 50 60 MIHTNLKKKF SLFILVFLLF AVICVWKKGS DYEALTLQAK EFQMPKSQEK VAMGSASQVV 70 80 90 100 110 120 FSNSKQDPKE DIPILSYHRV TAKVKPQPSF QVWDKDSTYS KLNPRLLKIW RNYLNMNKYK 130 140 150 160 170 180 VSYKGPGPGV KFSVEALRCH LRDHVNVSMI EATDFPFNTT EWEGYLPKEN FRTKVGPWQR 190 200 210 220 230 240 CAVVSSAGSL KNSQLGREID NHDAVLRFNG APTDNFQQDV GSKTTIRLMN SQLVTTEKRF 250 260 270 280 290 300 LKDSLYTEGI LIVWDPSVYH ADIPKWYQKP DYNFFETYKS YRRLNPSQPF YILKPQMPWE 310 320 330 340 350 360 LWDIIQEISA DLIQPNPPSS GMLGIIIMMT LCDQVDIYEF LPSKRKTDVC YYHQKFFDSA 370 380 390 400 CTMGAYDPLL FEKNMVKHLN EGTDEDIYLF GKATLSGFRN IRC
	Isoform RKA. Checksum: 1C05C601E3464550	FASTA	214	25,188
	10 20 30 40 50 60 MRYLLFWYGL PHSYSQCVCH WTPASGIFEN EPLLSLLLLV LGKLVTTEKR FLKDSLYTEG 70 80 90 100 110 120 ILIVWDPSVY HADIPKWYQK PDYNFFETYK SYRRLNPSQP FYILKPQMPW ELWDIIQEIS 130 140 150 160 170 180 ADLIQPNPPS SGMLGIIIMM TLCDQVDIYE FLPSKRKTDV CYYHQKFFDS ACTMGAYDPL 190 200 210 LFEKNMVKHL NEGTDEDIYL FGKATLSGFR NIRC
	Isoform RKB. Checksum: BC829880512B2F53	FASTA	184	21,874
	10 20 30 40 50 60 MRYLLFWYGL PHSLVTTEKR FLKDSLYTEG ILIVWDPSVY HADIPKWYQK PDYNFFETYK 70 80 90 100 110 120 SYRRLNPSQP FYILKPQMPW ELWDIIQEIS ADLIQPNPPS SGMLGIIIMM TLCDQVDIYE 130 140 150 160 170 180 FLPSKRKTDV CYYHQKFFDS ACTMGAYDPL LFEKNMVKHL NEGTDEDIYL FGKATLSGFR NIRC

References

[1]	"Primary structure of beta-galactoside alpha 2,6-sialyltransferase. Conversion of membrane-bound enzyme to soluble forms by cleavage of the NH2-terminal signal anchor." Weinstein J., Lee E.U., McEntee K., Lai P.-H., Paulson J.C. J. Biol. Chem. 262:17735-17743(1987) [PubMed: 3121604] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Rat beta-galactoside alpha 2,6-sialyltransferase genomic organization: alternate promoters direct the synthesis of liver and kidney transcripts." Wang X., O'Hanlon T.P., Young R.F., Lau J.T.Y. Glycobiology 1:25-31(1990) [PubMed: 1983783] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Kidney and Liver.
[3]	"Tissue-specific alternative splicing of the beta-galactoside alpha 2,6-sialyltransferase gene." Wen D.X., Svensson E.C., Paulson J.C. J. Biol. Chem. 267:2512-2518(1992) [PubMed: 1733948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney and Liver.
[4]	"Tissue-specific expression of beta-galactoside alpha-2,6-sialyltransferase. Transcript heterogeneity predicts a divergent polypeptide." O'Hanlon T.P., Lau K.M., Wang X., Lau J.T.Y. J. Biol. Chem. 264:17389-17394(1989) [PubMed: 2793863] [Abstract] Cited for: PARTIAL NUCLEOTIDE SEQUENCE (RKA). Strain: Sprague-Dawley. Tissue: Kidney.
[5]	"Conserved cysteines in the sialyltransferase sialylmotifs form an essential disulfide bond." Datta A.K., Chammas R., Paulson J.C. J. Biol. Chem. 276:15200-15207(2001) [PubMed: 11278697] [Abstract] Cited for: DISULFIDE BOND.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M18769 mRNA. Translation: AAA41196.1. M73987 mRNA. Translation: AAB06269.1. M83143 mRNA. Translation: AAB07233.1.
IPI	IPI00204392. IPI00231337. IPI00325330.
PIR	A28451. C42327.
RefSeq	NP_001106815.1. NP_671738.2.
UniGene	Rn.54567
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P13721.
Protein family/group databases
CAZy	GT29. Glycosyltransferase Family 29.
PTM databases
PhosphoSite	P13721.
Proteomic databases
PRIDE	P13721.
Genome annotation databases
Ensembl	ENSRNOT00000002496; ENSRNOP00000002496; ENSRNOG00000001823; Rattus norvegicus. [Genome view] ENSRNOT00000002499; ENSRNOP00000002499; ENSRNOG00000001823; Rattus norvegicus. [Genome view] ENSRNOT00000044591; ENSRNOP00000041089; ENSRNOG00000001823; Rattus norvegicus. [Genome view]
GeneID	25197.
KEGG	rno:25197.
UCSC	M18769. rat.
Organism-specific databases
CTD	25197.
RGD	3676. St6gal1.
Phylogenomic databases
HOVERGEN	P13721.
Enzyme and pathway databases
BRENDA	2.4.99.1. 248.
Gene expression databases
ArrayExpress	P13721.
Genevestigator	P13721.
GermOnline	ENSRNOG00000001823. Rattus norvegicus.
Family and domain databases
InterPro	IPR001675. Glyco_trans_29. IPR012163. Sialyl_trans. [Graphical view]
Pfam	PF00777. Glyco_transf_29. 1 hit. [Graphical view]
PIRSF	PIRSF005557. Sialyl_trans. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	605715.

Entry information

Entry name

SIAT1_RAT

Accession

Primary (citable) accession number: P13721

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	October 13, 2009
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)