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P13714 (LDH_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
L-lactate dehydrogenase
Short name=L-LDH
EC=1.1.1.27
Gene names
Name:ldh
Synonyms:lctE
Ordered Locus Names:BSU03050
OrganismBacillus subtilis
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length320 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

(S)-lactate + NAD+ = pyruvate + NADH. HAMAP MF_00488

Pathway

Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. HAMAP MF_00488

Subunit structure

Homotetramer.

Subcellular location

Cytoplasm HAMAP MF_00488.

Induction

Strongly induced under anaerobic conditions. Activated by ResDE, Fnr and ArfM. Ref.5

Sequence similarities

Belongs to the LDH/MDH superfamily. LDH family.

Sequence caution

The sequence BAA08939.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAB12099.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandNAD
   Molecular functionOxidoreductase
   PTMPhosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological processglycolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionL-lactate dehydrogenase activity

Inferred from electronic annotation. Source: EC

nucleotide binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 320320L-lactate dehydrogenase HAMAP MF_00488
PRO_0000168329

Regions

Nucleotide binding14 – 4229NAD By similarity

Sites

Active site1781Proton acceptor By similarity
Binding site911Substrate By similarity
Binding site1231NAD or substrate By similarity
Binding site1541Substrate By similarity
Binding site2321Substrate By similarity

Amino acid modifications

Modified residue2231Phosphotyrosine Ref.6

Experimental info

Sequence conflict381V → L AA sequence Ref.4
Sequence conflict511N → P in BAA08939. Ref.1
Sequence conflict57 – 582AP → GL in BAA08939. Ref.1
Sequence conflict1201V → I AA sequence Ref.4
Sequence conflict2241H → T AA sequence Ref.4
Sequence conflict315 – 3184Missing AA sequence Ref.4

Sequences

Sequence LengthMass (Da)Tools
P13714 [UniParc].

Last modified May 26, 2009. Version 3.
Checksum: F0B71F5A760FCAE1

FASTA32034,802
        10         20         30         40         50         60 
MNKHVNKVAL IGAGFVGSSY AFALINQGIT DELVVIDVNK EKAMGDVMDL NHGKAFAPQP 

        70         80         90        100        110        120 
VKTSYGTYED CKDADIVCIC AGANQKPGET RLELVEKNLK IFKGIVSEVM ASGFDGIFLV 

       130        140        150        160        170        180 
ATNPVDILTY ATWKFSGLPK ERVIGSGTTL DSARFRFMLS EYFGAAPQNV HAHIIGEHGD 

       190        200        210        220        230        240 
TELPVWSHAN VGGVPVSELV EKNDAYKQEE LDQIVDDVKN AAYHIIEKKG ATYYGVAMSL 

       250        260        270        280        290        300 
ARITKAILHN ENSILTVSTY LDGQYGADDV YIGVPAVVNR GGIAGITELN LNEKEKEQFL 

       310        320 
HSAGVLKNIL KPHFAEQKVN

« Hide

References

« Hide 'large scale' references
[1]"The 25 degrees-36 degrees region of the Bacillus subtilis chromosome: determination of the sequence of a 146 kb segment and identification of 113 genes."
Yamane K., Kumano M., Kurita K.
Microbiology 142:3047-3056(1996) [PubMed: 8969502] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract]
Cited for: SEQUENCE REVISION TO 51 AND 57-58.
[4]"Structure and function of L-lactate dehydrogenases from thermophilic and mesophilic bacteria, IV. The primary structure of the mesophilic lactate dehydrogenase from Bacillus subtilis."
Hediger M.A., Frank G., Zuber H.
Biol. Chem. Hoppe-Seyler 367:891-903(1986) [PubMed: 3098260] [Abstract]
Cited for: PROTEIN SEQUENCE.
[5]"Fermentative metabolism of Bacillus subtilis: physiology and regulation of gene expression."
Cruz Ramos H., Hoffmann T., Marino M., Nedjari H., Presecan-Siedel E., Dreesen O., Glaser P., Jahn D.
J. Bacteriol. 182:3072-3080(2000) [PubMed: 10809684] [Abstract]
Cited for: REGULATION.
[6]"The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis."
Macek B., Mijakovic I., Olsen J.V., Gnad F., Kumar C., Jensen P.R., Mann M.
Mol. Cell. Proteomics 6:697-707(2007) [PubMed: 17218307] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-223, MASS SPECTROMETRY.
Strain: 168.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D50453 Genomic DNA. Translation: BAA08939.1. Different initiation.
AL009126 Genomic DNA. Translation: CAB12099.2. Different initiation.
PIRE69649.
RefSeqNP_388187.2. NC_000964.3.

3D structure databases

ProteinModelPortalP13714.
SMRP13714. Positions 7-314.
ModBaseSearch...

PTM databases

PhosSiteP13714.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000003920; EBBACP00000003920; EBBACG00000003912.
GeneID938348.
GenomeReviewsGene locus BSU03050 in contig AL009126_GR.
KEGGbsu:BSU03050.
NMPDRfig|224308.1.peg.306.
PATRIC18972169. VBIBacSub10457_0313.

Organism-specific databases

GenoListBSU03050. [Micado]

Phylogenomic databases

GeneTreeEBGT00050000001153.
HOGENOMHBG566126.
PhylomeDBP13714.
ProtClustDBPRK00066.

Enzyme and pathway databases

BioCycBSUB:BSU03050-MONOMER.

Family and domain databases

HAMAPMF_00488. Lactate_dehydrog.
[Tree]
InterProIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.90.110.10. lact_mal_DH. 1 hit.
G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KOK00016.
PANTHERPTHR11540:SF3. PTHR11540:SF3. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSPR00086. LLDHDRGNASE.
SUPFAMSSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMsTIGR01771. L-LDH-NAD. 1 hit.
PROSITEPS00064. L_LDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLDH_BACSU
AccessionPrimary (citable) accession number: P13714
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: May 26, 2009
Last modified: January 25, 2012
This is version 109 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents