P13712 (MSI1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chromatin assembly factor 1 subunit p50
Short name=CAF-1 p50 subunit

Alternative name(s):
IRA1 multicopy suppressor
Protein MSI1

Gene names

Name:	MSI1
Synonyms:	CAC3
Ordered Locus Names:	YBR195C
ORF Names:	YBR1405

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	422 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a component of chromatin assembly factor 1 (CAF-1), which assembles histone octamers onto replicating DNA in vitro. It performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. p50 and p60 form complexes with newly synthesized histones H3 and acetylated H4 in cell extracts By similarity. Independently, MSI1 is involved in regulation of the RAS/cAMP pathway via sequestration of NPR1. Ref.7
Subunit structure	Interacts with protein kinase NPR1. Component of chromatin assembly factor 1 (CAF-1), which is composed of MSI1/p50, CAC2/p60 and CAC1/p90. Ref.7
Subcellular location	Cytoplasm. Nucleus Ref.7.
Miscellaneous	Present with 149 molecules/cell in log phase SD medium. Ref.8
Sequence similarities	Belongs to the WD repeat RBAP46/RBAP48/MSI1 family. Contains 6 WD repeats.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Domain	Repeat WD repeat
Ligand	cAMP
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	DNA replication-dependent nucleosome assembly Inferred from direct assay Ref.6. Source: SGD
Cellular component	CAF-1 complex Inferred from direct assay Ref.6. Source: SGD cytoplasm Inferred from direct assay Ref.7. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RLF2	Q12495	5	EBI-11391,EBI-3913

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 422	422	Chromatin assembly factor 1 subunit p50		PRO_0000051086
Regions
	Repeat	127 – 158	32	WD 1
	Repeat	198 – 238	41	WD 2
	Repeat	249 – 289	41	WD 3
	Repeat	294 – 334	41	WD 4
	Repeat	338 – 379	42	WD 5
	Repeat	382 – 421	40	WD 6
Amino acid modifications
	Modified residue	14	1	Phosphoserine Ref.9

Sequences

	Sequence		Length	Mass (Da)
	P13712 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: 0D3DB6CB2AC74166	FASTA	422	47,365
	10 20 30 40 50 60 MNQCAKDITH EASSIPIDLQ ERYSHWKKNT KLLYDYLNTN STKWPSLTCQ FFPDLDTTSD 70 80 90 100 110 120 EHRILLSSFT SSQKPEDETI YISKISTLGH IKWSSLNNFD MDEMEFKPEN STRFPSKHLV 130 140 150 160 170 180 NDISIFFPNG ECNRARYLPQ NPDIIAGASS DGAIYIFDRT KHGSTRIRQS KISHPFETKL 190 200 210 220 230 240 FGSHGVIQDV EAMDTSSADI NEATSLAWNL QQEALLLSSH SNGQVQVWDI KQYSHENPII 250 260 270 280 290 300 DLPLVSINSD GTAVNDVTWM PTHDSLFAAC TEGNAVSLLD LRTKKEKLQS NREKHDGGVN 310 320 330 340 350 360 SCRFNYKNSL ILASADSNGR LNLWDIRNMN KSPIATMEHG TSVSTLEWSP NFDTVLATAG 370 380 390 400 410 420 QEDGLVKLWD TSCEETIFTH GGHMLGVNDI SWDAHDPWLM CSVANDNSVH IWKPAGNLVG HS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"MSI1, a negative regulator of the RAS-cAMP pathway in Saccharomyces cerevisiae." Ruggieri R., Tanaka K., Nakafuku M., Kaziro Y., Toh-e A., Matsumoto K. Proc. Natl. Acad. Sci. U.S.A. 86:8778-8782(1989) [PubMed: 2554329] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"RIM2, MSI1 and PGI1 are located within an 8 kb segment of Saccharomyces cerevisiae chromosome II, which also contains the putative ribosomal gene L21 and a new putative essential gene with a leucine zipper motif." Demolis N., Mallet L., Bussereau F., Jacquet M. Yeast 9:645-659(1993) [PubMed: 8346681] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Ultraviolet radiation sensitivity and reduction of telomeric silencing in Saccharomyces cerevisiae cells lacking chromatin assembly factor-I." Kaufman P.D., Kobayashi R., Stillman B. Genes Dev. 11:345-357(1997) [PubMed: 9030687] [Abstract] Cited for: PROTEIN SEQUENCE OF 7-27; 32-43 AND 414-422, CHARACTERIZATION.
[7]	"CAC3(MSI1) suppression of RAS2(G19V) is independent of chromatin assembly factor I and mediated by NPR1." Johnston S.D., Enomoto S., Schneper L., McClellan M.C., Twu F., Montgomery N.D., Haney S.A., Broach J.R., Berman J. Mol. Cell. Biol. 21:1784-1794(2001) [PubMed: 11238915] [Abstract] Cited for: FUNCTION, INTERACTION WITH NPR1, SUBCELLULAR LOCATION.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-14, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M27300 Genomic DNA. Translation: AAA34804.1. Z21487 Genomic DNA. Translation: CAA79682.1. Z36064 Genomic DNA. Translation: CAA85157.1. AY692834 Genomic DNA. Translation: AAT92853.1. BK006936 Genomic DNA. Translation: DAA07309.1.
PIR	BVBYMS. S07865.
RefSeq	NP_009754.1. NM_001178543.1.
3D structure databases
ProteinModelPortal	P13712.
SMR	P13712. Positions 1-420.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-338N.
IntAct	P13712. 11 interactions.
MINT	MINT-386252.
STRING	P13712.
Proteomic databases
PeptideAtlas	P13712.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR195C; YBR195C; YBR195C.
GeneID	852494.
KEGG	sce:YBR195C.
NMPDR	fig|4932.3.peg.462.
Organism-specific databases
CYGD	YBR195c.
SGD	S000000399. MSI1.
Phylogenomic databases
eggNOG	fuNOG05637.
GeneTree	EFGT00050000000073.
HOGENOM	HBG396292.
OMA	DYLNTNS.
OrthoDB	EOG44TSHP.
Gene expression databases
ArrayExpress	P13712.
Genevestigator	P13712.
GermOnline	YBR195C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR022052. Histone-bd_RBBP4. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR011046. WD40_repeat-like_dom. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
Pfam	PF12265. CAF1C_H4-bd. 1 hit. PF00400. WD40. 3 hits. [Graphical view]
SMART	SM00320. WD40. 6 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 1 hit.
PROSITE	PS00678. WD_REPEATS_1. 3 hits. PS50082. WD_REPEATS_2. 2 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	971486.

Entry information

Entry name

MSI1_YEAST

Accession

Primary (citable) accession number: P13712
Secondary accession number(s): D6VQI9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	January 25, 2012
This is version 119 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program