Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P13707 (GPDA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 138. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic

Short name=GPD-C
Short name=GPDH-C
EC=1.1.1.8
Gene names
Name:Gpd1
Synonyms:Gdc-1, Gdc1, Kiaa4010
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length349 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.

Subunit structure

Homodimer.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.

Sequence caution

The sequence BAD90479.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandNAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processNADH oxidation

Inferred from electronic annotation. Source: Ensembl

cellular response to cAMP

Inferred from direct assay Ref.1. Source: MGI

cellular response to tumor necrosis factor

Inferred from direct assay Ref.1. Source: MGI

gluconeogenesis

Inferred from mutant phenotype PubMed 12093800. Source: MGI

glycerol-3-phosphate catabolic process

Inferred from electronic annotation. Source: InterPro

glycerol-3-phosphate metabolic process

Inferred from mutant phenotype PubMed 12093800. Source: MGI

glycerolipid metabolic process

Inferred from electronic annotation. Source: Ensembl

glycerophosphate shuttle

Inferred from mutant phenotype PubMed 11147825. Source: MGI

positive regulation of glycolytic process

Inferred from mutant phenotype PubMed 11147825. Source: MGI

   Cellular_componentcytosol

Inferred from electronic annotation. Source: Ensembl

glycerol-3-phosphate dehydrogenase complex

Traceable author statement PubMed 12093800. Source: MGI

mitochondrion

Inferred from direct assay PubMed 18614015. Source: MGI

   Molecular_functionNAD binding

Inferred from electronic annotation. Source: Ensembl

glycerol-3-phosphate dehydrogenase [NAD+] activity

Inferred from direct assay PubMed 11147825PubMed 6105153. Source: MGI

glycerol-3-phosphate dehydrogenase activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 349349Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic
PRO_0000138080

Regions

Nucleotide binding10 – 156NAD By similarity
Region269 – 2702Substrate binding By similarity

Sites

Active site2041Proton acceptor Potential
Binding site411NAD By similarity
Binding site971NAD By similarity
Binding site1201Substrate By similarity
Binding site1531NAD; via amide nitrogen By similarity
Binding site2691NAD By similarity
Binding site2961NAD; via amide nitrogen By similarity
Binding site2981NAD By similarity

Amino acid modifications

Modified residue2891N6-succinyllysine Ref.8

Experimental info

Sequence conflict2181G → R AA sequence Ref.7
Sequence conflict2461T → I in AAA37726. Ref.2
Sequence conflict2461T → I in AAA37727. Ref.2
Sequence conflict2461T → I in BAD90479. Ref.4
Sequence conflict3181K → R in BAE23246. Ref.5

Sequences

Sequence LengthMass (Da)Tools
P13707 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: F2C572DADFEA1B99

FASTA34937,573
        10         20         30         40         50         60 
MAGKKVCIVG SGNWGSAIAK IVGSNAGRLA HFDPRVTMWV FEEDIGGRKL TEIINTQHEN 

        70         80         90        100        110        120 
VKYLPGHKLP PNVVAIPDVV QAATGADILV FVVPHQFIGK ICDQLKGHLK ANTIGISLIK 

       130        140        150        160        170        180 
GVDEGPNGLK LISEVIGERL GIPMSVLMGA NIASEVAEEK FCETTIGCKD PAQGQLLKDL 

       190        200        210        220        230        240 
MQTPNFRITV VQEVDTVEIC GALKNIVAVG AGFCDGLGFG DNTKAAVIRL GLMEMIAFAK 

       250        260        270        280        290        300 
LFCSGTVSSA TFLESCGVAD LITTCYGGRN RKVAEAFART GKSIEQLEKE MLNGQKLQGP 

       310        320        330        340 
QTARELHSIL QHKGLVDKFP LFTAVYKVCY EGQPVGEFIR CLQNHPEHM 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence and hormonal regulation of mouse glycerophosphate dehydrogenase mRNA during adipocyte and muscle cell differentiation."
Dobson D.E., Groves D.L., Spiegelman B.M.
J. Biol. Chem. 262:1804-1809(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Primary structure of the mouse glycerol-3-phosphate dehydrogenase gene."
Ireland R.C., Kotarski M.A., Johnston L.A., Stadler U., Birkenmeier E., Kozak L.P.
J. Biol. Chem. 261:11779-11785(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The nucleotide sequence of three genes participating in the adipose differentiation of 3T3 cells."
Phillips M., Djian P., Green H.
J. Biol. Chem. 261:10821-10827(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[5]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Kidney, Lung and Urinary bladder.
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Liver.
[7]Lubec G., Klug S., Kang S.U.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 50-62; 111-130; 205-218; 230-240; 297-304 AND 319-340, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: C57BL/6.
Tissue: Brain and Hippocampus.
[8]"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-289, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J02655 mRNA. Translation: AAA37728.1.
M25558 Genomic DNA. Translation: AAA37726.1.
M13366 Genomic DNA. Translation: AAA37727.1.
AK220445 mRNA. Translation: BAD90479.1. Different initiation.
AK137134 mRNA. Translation: BAE23246.1.
AK004565 mRNA. Translation: BAB23376.1.
AK018733 mRNA. Translation: BAB31376.1.
BC005756 mRNA. Translation: AAH05756.1.
BC019391 mRNA. Translation: AAH19391.1.
CCDSCCDS27828.1.
PIRA25952.
RefSeqNP_034401.1. NM_010271.2.
UniGeneMm.252391.

3D structure databases

ProteinModelPortalP13707.
SMRP13707. Positions 1-349.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP13707. 7 interactions.
MINTMINT-1855802.

Chemistry

ChEMBLCHEMBL1075171.

PTM databases

PhosphoSiteP13707.

2D gel databases

REPRODUCTION-2DPAGEIPI00230185.
P13707.
SWISS-2DPAGEP13707.

Proteomic databases

MaxQBP13707.
PaxDbP13707.
PRIDEP13707.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000023760; ENSMUSP00000023760; ENSMUSG00000023019.
GeneID14555.
KEGGmmu:14555.
UCSCuc007xqd.1. mouse.

Organism-specific databases

CTD2819.
MGIMGI:95679. Gpd1.
RougeSearch...

Phylogenomic databases

eggNOGCOG0240.
GeneTreeENSGT00390000003114.
HOGENOMHOG000246855.
HOVERGENHBG003669.
InParanoidP13707.
KOK00006.
OMAPVGEFIH.
OrthoDBEOG7ZKSBS.
PhylomeDBP13707.
TreeFamTF300836.

Enzyme and pathway databases

SABIO-RKP13707.

Gene expression databases

ArrayExpressP13707.
BgeeP13707.
CleanExMM_GPD1.
GenevestigatorP13707.

Family and domain databases

Gene3D1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProIPR008927. 6-PGluconate_DH_C-like.
IPR013328. DH_multihelical.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR017751. G3P_DH_NAD-dep_euk.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR11728. PTHR11728. 1 hit.
PfamPF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view]
PIRSFPIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTSPR00077. GPDHDRGNASE.
SUPFAMSSF48179. SSF48179. 1 hit.
TIGRFAMsTIGR03376. glycerol3P_DH. 1 hit.
PROSITEPS00957. NAD_G3PDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSGPD1. mouse.
NextBio286250.
PROP13707.
SOURCESearch...

Entry information

Entry nameGPDA_MOUSE
AccessionPrimary (citable) accession number: P13707
Secondary accession number(s): Q3UVM3, Q5DTS5
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 138 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot