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P13707

- GPDA_MOUSE

UniProt

P13707 - GPDA_MOUSE

Protein

Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic

Gene

Gpd1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 140 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei41 – 411NADBy similarity
    Binding sitei97 – 971NADBy similarity
    Binding sitei120 – 1201SubstrateBy similarity
    Binding sitei153 – 1531NAD; via amide nitrogenBy similarity
    Active sitei204 – 2041Proton acceptorSequence Analysis
    Binding sitei269 – 2691NADBy similarity
    Binding sitei296 – 2961NAD; via amide nitrogenBy similarity
    Binding sitei298 – 2981NADBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 156NADBy similarity

    GO - Molecular functioni

    1. glycerol-3-phosphate dehydrogenase [NAD+] activity Source: MGI
    2. glycerol-3-phosphate dehydrogenase activity Source: Ensembl
    3. NAD binding Source: Ensembl

    GO - Biological processi

    1. cellular response to cAMP Source: MGI
    2. cellular response to tumor necrosis factor Source: MGI
    3. gluconeogenesis Source: MGI
    4. glycerol-3-phosphate catabolic process Source: InterPro
    5. glycerol-3-phosphate metabolic process Source: MGI
    6. glycerolipid metabolic process Source: Ensembl
    7. glycerophosphate shuttle Source: MGI
    8. NADH oxidation Source: Ensembl
    9. positive regulation of glycolytic process Source: MGI

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    ReactomeiREACT_188640. Synthesis of PA.
    SABIO-RKP13707.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic (EC:1.1.1.8)
    Short name:
    GPD-C
    Short name:
    GPDH-C
    Gene namesi
    Name:Gpd1
    Synonyms:Gdc-1, Gdc1, Kiaa4010
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 15

    Organism-specific databases

    MGIiMGI:95679. Gpd1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: Ensembl
    2. glycerol-3-phosphate dehydrogenase complex Source: MGI
    3. mitochondrion Source: MGI

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 349349Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmicPRO_0000138080Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei289 – 2891N6-succinyllysine1 Publication

    Proteomic databases

    MaxQBiP13707.
    PaxDbiP13707.
    PRIDEiP13707.

    2D gel databases

    REPRODUCTION-2DPAGEIPI00230185.
    P13707.
    SWISS-2DPAGEP13707.

    PTM databases

    PhosphoSiteiP13707.

    Expressioni

    Gene expression databases

    ArrayExpressiP13707.
    BgeeiP13707.
    CleanExiMM_GPD1.
    GenevestigatoriP13707.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    IntActiP13707. 7 interactions.
    MINTiMINT-1855802.

    Structurei

    3D structure databases

    ProteinModelPortaliP13707.
    SMRiP13707. Positions 1-349.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni269 – 2702Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0240.
    GeneTreeiENSGT00390000003114.
    HOGENOMiHOG000246855.
    HOVERGENiHBG003669.
    InParanoidiP13707.
    KOiK00006.
    OMAiPVGEFIH.
    OrthoDBiEOG7ZKSBS.
    PhylomeDBiP13707.
    TreeFamiTF300836.

    Family and domain databases

    Gene3Di1.10.1040.10. 1 hit.
    3.40.50.720. 1 hit.
    InterProiIPR008927. 6-PGluconate_DH_C-like.
    IPR013328. DH_multihelical.
    IPR006168. G3P_DH_NAD-dep.
    IPR006109. G3P_DH_NAD-dep_C.
    IPR017751. G3P_DH_NAD-dep_euk.
    IPR011128. G3P_DH_NAD-dep_N.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11728. PTHR11728. 1 hit.
    PfamiPF07479. NAD_Gly3P_dh_C. 1 hit.
    PF01210. NAD_Gly3P_dh_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000114. Glycerol-3-P_dh. 1 hit.
    PRINTSiPR00077. GPDHDRGNASE.
    SUPFAMiSSF48179. SSF48179. 1 hit.
    TIGRFAMsiTIGR03376. glycerol3P_DH. 1 hit.
    PROSITEiPS00957. NAD_G3PDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P13707-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGKKVCIVG SGNWGSAIAK IVGSNAGRLA HFDPRVTMWV FEEDIGGRKL    50
    TEIINTQHEN VKYLPGHKLP PNVVAIPDVV QAATGADILV FVVPHQFIGK 100
    ICDQLKGHLK ANTIGISLIK GVDEGPNGLK LISEVIGERL GIPMSVLMGA 150
    NIASEVAEEK FCETTIGCKD PAQGQLLKDL MQTPNFRITV VQEVDTVEIC 200
    GALKNIVAVG AGFCDGLGFG DNTKAAVIRL GLMEMIAFAK LFCSGTVSSA 250
    TFLESCGVAD LITTCYGGRN RKVAEAFART GKSIEQLEKE MLNGQKLQGP 300
    QTARELHSIL QHKGLVDKFP LFTAVYKVCY EGQPVGEFIR CLQNHPEHM 349
    Length:349
    Mass (Da):37,573
    Last modified:January 23, 2007 - v3
    Checksum:iF2C572DADFEA1B99
    GO

    Sequence cautioni

    The sequence BAD90479.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti218 – 2181G → R AA sequence 1 PublicationCurated
    Sequence conflicti246 – 2461T → I in AAA37726. (PubMed:3755721)Curated
    Sequence conflicti246 – 2461T → I in AAA37727. (PubMed:3755721)Curated
    Sequence conflicti246 – 2461T → I in BAD90479. 1 PublicationCurated
    Sequence conflicti318 – 3181K → R in BAE23246. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02655 mRNA. Translation: AAA37728.1.
    M25558 Genomic DNA. Translation: AAA37726.1.
    M13366 Genomic DNA. Translation: AAA37727.1.
    AK220445 mRNA. Translation: BAD90479.1. Different initiation.
    AK137134 mRNA. Translation: BAE23246.1.
    AK004565 mRNA. Translation: BAB23376.1.
    AK018733 mRNA. Translation: BAB31376.1.
    BC005756 mRNA. Translation: AAH05756.1.
    BC019391 mRNA. Translation: AAH19391.1.
    CCDSiCCDS27828.1.
    PIRiA25952.
    RefSeqiNP_034401.1. NM_010271.2.
    UniGeneiMm.252391.

    Genome annotation databases

    EnsembliENSMUST00000023760; ENSMUSP00000023760; ENSMUSG00000023019.
    GeneIDi14555.
    KEGGimmu:14555.
    UCSCiuc007xqd.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02655 mRNA. Translation: AAA37728.1 .
    M25558 Genomic DNA. Translation: AAA37726.1 .
    M13366 Genomic DNA. Translation: AAA37727.1 .
    AK220445 mRNA. Translation: BAD90479.1 . Different initiation.
    AK137134 mRNA. Translation: BAE23246.1 .
    AK004565 mRNA. Translation: BAB23376.1 .
    AK018733 mRNA. Translation: BAB31376.1 .
    BC005756 mRNA. Translation: AAH05756.1 .
    BC019391 mRNA. Translation: AAH19391.1 .
    CCDSi CCDS27828.1.
    PIRi A25952.
    RefSeqi NP_034401.1. NM_010271.2.
    UniGenei Mm.252391.

    3D structure databases

    ProteinModelPortali P13707.
    SMRi P13707. Positions 1-349.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P13707. 7 interactions.
    MINTi MINT-1855802.

    Chemistry

    ChEMBLi CHEMBL1075171.

    PTM databases

    PhosphoSitei P13707.

    2D gel databases

    REPRODUCTION-2DPAGE IPI00230185.
    P13707.
    SWISS-2DPAGE P13707.

    Proteomic databases

    MaxQBi P13707.
    PaxDbi P13707.
    PRIDEi P13707.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000023760 ; ENSMUSP00000023760 ; ENSMUSG00000023019 .
    GeneIDi 14555.
    KEGGi mmu:14555.
    UCSCi uc007xqd.1. mouse.

    Organism-specific databases

    CTDi 2819.
    MGIi MGI:95679. Gpd1.
    Rougei Search...

    Phylogenomic databases

    eggNOGi COG0240.
    GeneTreei ENSGT00390000003114.
    HOGENOMi HOG000246855.
    HOVERGENi HBG003669.
    InParanoidi P13707.
    KOi K00006.
    OMAi PVGEFIH.
    OrthoDBi EOG7ZKSBS.
    PhylomeDBi P13707.
    TreeFami TF300836.

    Enzyme and pathway databases

    Reactomei REACT_188640. Synthesis of PA.
    SABIO-RK P13707.

    Miscellaneous databases

    ChiTaRSi GPD1. mouse.
    NextBioi 286250.
    PROi P13707.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P13707.
    Bgeei P13707.
    CleanExi MM_GPD1.
    Genevestigatori P13707.

    Family and domain databases

    Gene3Di 1.10.1040.10. 1 hit.
    3.40.50.720. 1 hit.
    InterProi IPR008927. 6-PGluconate_DH_C-like.
    IPR013328. DH_multihelical.
    IPR006168. G3P_DH_NAD-dep.
    IPR006109. G3P_DH_NAD-dep_C.
    IPR017751. G3P_DH_NAD-dep_euk.
    IPR011128. G3P_DH_NAD-dep_N.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11728. PTHR11728. 1 hit.
    Pfami PF07479. NAD_Gly3P_dh_C. 1 hit.
    PF01210. NAD_Gly3P_dh_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000114. Glycerol-3-P_dh. 1 hit.
    PRINTSi PR00077. GPDHDRGNASE.
    SUPFAMi SSF48179. SSF48179. 1 hit.
    TIGRFAMsi TIGR03376. glycerol3P_DH. 1 hit.
    PROSITEi PS00957. NAD_G3PDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence and hormonal regulation of mouse glycerophosphate dehydrogenase mRNA during adipocyte and muscle cell differentiation."
      Dobson D.E., Groves D.L., Spiegelman B.M.
      J. Biol. Chem. 262:1804-1809(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Primary structure of the mouse glycerol-3-phosphate dehydrogenase gene."
      Ireland R.C., Kotarski M.A., Johnston L.A., Stadler U., Birkenmeier E., Kozak L.P.
      J. Biol. Chem. 261:11779-11785(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The nucleotide sequence of three genes participating in the adipose differentiation of 3T3 cells."
      Phillips M., Djian P., Green H.
      J. Biol. Chem. 261:10821-10827(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
      Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    5. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Kidney, Lung and Urinary bladder.
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Liver.
    7. Lubec G., Klug S., Kang S.U.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 50-62; 111-130; 205-218; 230-240; 297-304 AND 319-340, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6.
      Tissue: Brain and Hippocampus.
    8. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-289, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiGPDA_MOUSE
    AccessioniPrimary (citable) accession number: P13707
    Secondary accession number(s): Q3UVM3, Q5DTS5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 140 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3