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Protein

Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic

Gene

Gpdh

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.

Pathwayi: alpha-glycerophosphate cycle

This protein is involved in the pathway alpha-glycerophosphate cycle, which is part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the pathway alpha-glycerophosphate cycle and in Phospholipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei98NADBy similarity1
Binding sitei121NAD; via amide nitrogenBy similarity1
Binding sitei121SubstrateBy similarity1
Binding sitei155NAD; via amide nitrogenBy similarity1
Active sitei206Proton acceptorBy similarity1
Binding sitei270NADBy similarity1
Binding sitei299NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 16NADBy similarity6

GO - Molecular functioni

GO - Biological processi

  • carbohydrate metabolic process Source: InterPro
  • ethanol metabolic process Source: CACAO
  • flight behavior Source: FlyBase
  • glycerol-3-phosphate catabolic process Source: InterPro
  • glycerophospholipid metabolic process Source: UniProtKB-UniPathway
  • triglyceride metabolic process Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiR-DME-1483166. Synthesis of PA.
R-DME-75109. Triglyceride Biosynthesis.
UniPathwayiUPA00086.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic (EC:1.1.1.8)
Short name:
GPD-C
Short name:
GPDH-C
Gene namesi
Name:Gpdh
ORF Names:CG9042
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0001128. Gpdh.

Subcellular locationi

GO - Cellular componenti

  • glycerol-3-phosphate dehydrogenase complex Source: InterPro
  • lipid particle Source: FlyBase
  • M band Source: FlyBase
  • Z disc Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001380751 – 363Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmicAdd BLAST363

Proteomic databases

PaxDbiP13706.
PRIDEiP13706.

Expressioni

Tissue specificityi

Isoform GPDH-1 is predominant in thorax and isoform GPDH-3 in abdomen.

Developmental stagei

Isoform GPDH-2 and isoform GPDH-3 are expressed in both larvae and adults. Isoform GPDH-1 is expressed only in adults.2 Publications

Gene expression databases

BgeeiFBgn0001128.
GenevisibleiP13706. DM.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi59989. 66 interactors.
DIPiDIP-21689N.
IntActiP13706. 1 interactor.
STRINGi7227.FBpp0305431.

Structurei

3D structure databases

ProteinModelPortaliP13706.
SMRiP13706.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni270 – 271Substrate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2711. Eukaryota.
COG0240. LUCA.
InParanoidiP13706.
KOiK00006.
OMAiRNHTAGR.
OrthoDBiEOG091G0DO6.
PhylomeDBiP13706.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR017751. G3P_DH_NAD-dep_euk.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTSiPR00077. GPDHDRGNASE.
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR03376. glycerol3P_DH. 1 hit.
PROSITEiPS00957. NAD_G3PDH. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform GPDH-4 (identifier: P13706-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADKVNVCIV GSGNWGSAIA KIVGANAAAL PEFEERVTMF VYEELIDGKK
60 70 80 90 100
LTEIINETHE NVKYLKGHKL PPNVVAVPDL VEAAKNADIL IFVVPHQFIP
110 120 130 140 150
NFCKQLLGKI KPNAIAISLI KGFDKAEGGG IDLISHIITR HLKIPCAVLM
160 170 180 190 200
GANLANEVAE GNFCETTIGC TDKKYGKVLR DLFQANHFRV VVVDDADAVE
210 220 230 240 250
VCGALKNIVA CGAGFVDGLK LGDNTKAAVI RLGLMEMIRF VDVFYPGSKL
260 270 280 290 300
STFFESCGVA DLITTCYGGR NRRVSEAFVT SGKTIEELEK EMLNGQKLQG
310 320 330 340 350
PPTAEEVNYM LKNKGLEDKF PLFTAIHKIC TNQLKPNDLI DCIRNHPEHM
360
DTSIMPSPKL QNL
Length:363
Mass (Da):39,684
Last modified:June 1, 2001 - v3
Checksum:i49FA09CE7FB6FDF4
GO
Isoform GPDH-1 (identifier: P13706-2) [UniParc]FASTAAdd to basket
Also known as: GPDH-411, C

The sequence of this isoform differs from the canonical sequence as follows:
     351-360: Missing.

Show »
Length:353
Mass (Da):38,614
Checksum:i629616247F906ACF
GO
Isoform GPDH-2 (identifier: P13706-3) [UniParc]FASTAAdd to basket
Also known as: GPDH-37, B

The sequence of this isoform differs from the canonical sequence as follows:
     361-363: Missing.

Show »
Length:360
Mass (Da):39,329
Checksum:i7E7FB6FDF432CDD0
GO
Isoform GPDH-3 (identifier: P13706-4) [UniParc]FASTAAdd to basket
Also known as: GPDH-1A, A

The sequence of this isoform differs from the canonical sequence as follows:
     351-363: Missing.

Show »
Length:350
Mass (Da):38,259
Checksum:iB47F906ACF061F8E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti351 – 363DTSIM…KLQNL → GYVHHAVAKTCKICKSPKEA ETFLTFLCTTENKCIFSAQR QLDESLDSIF in CAA47892 (PubMed:8094564).CuratedAdd BLAST13

Polymorphismi

There are two common alleles; fast and slow. The sequence of fast is shown here.

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti14N → Y in allele GPDH-ACYG22. 1
Natural varianti266C → G in allele GPDH-ACB62. 1
Natural varianti273R → C in allele GPDH-ACYG22. 1
Natural varianti337N → K in allele GPDH-AT198, allele GPDH-ACB62 and allele GPDH-S. 1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_001590351 – 363Missing in isoform GPDH-3. CuratedAdd BLAST13
Alternative sequenceiVSP_001589351 – 360Missing in isoform GPDH-1. Curated10
Alternative sequenceiVSP_001591361 – 363Missing in isoform GPDH-2. 1 Publication3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04567 Genomic DNA. Translation: AAA28591.1.
J04567 Genomic DNA. Translation: AAA28592.1.
J04567 Genomic DNA. Translation: AAA28593.1.
X14179 Genomic DNA. Translation: CAA32379.1.
X14179 Genomic DNA. Translation: CAA32380.1.
X14179 Genomic DNA. Translation: CAA32381.1.
X67650 Genomic DNA. Translation: CAA47892.1.
X61223 Genomic DNA. No translation available.
X61224 Genomic DNA. Translation: CAA43536.1.
AE014134 Genomic DNA. Translation: AAF52304.1.
AE014134 Genomic DNA. Translation: AAN10562.1.
AE014134 Genomic DNA. Translation: AAN10563.1.
AY058492 mRNA. Translation: AAL13721.1.
X80204 Genomic DNA. Translation: CAA56497.1.
M13786 Genomic DNA. Translation: AAA28590.1.
J03927 Genomic DNA. Translation: AAA28553.1.
J03927 Genomic DNA. Translation: AAA28554.1.
J03927 Genomic DNA. Translation: AAA28555.1.
PIRiS06758.
S06759.
S06760.
S21963.
RefSeqiNP_001260111.1. NM_001273182.1. [P13706-4]
NP_001260112.1. NM_001273183.2. [P13706-3]
NP_001260113.1. NM_001273184.2. [P13706-2]
NP_476565.1. NM_057217.4. [P13706-3]
NP_476566.1. NM_057218.4. [P13706-2]
NP_476567.1. NM_057219.4. [P13706-4]
UniGeneiDm.4843.

Genome annotation databases

GeneIDi33824.
KEGGidme:Dmel_CG9042.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04567 Genomic DNA. Translation: AAA28591.1.
J04567 Genomic DNA. Translation: AAA28592.1.
J04567 Genomic DNA. Translation: AAA28593.1.
X14179 Genomic DNA. Translation: CAA32379.1.
X14179 Genomic DNA. Translation: CAA32380.1.
X14179 Genomic DNA. Translation: CAA32381.1.
X67650 Genomic DNA. Translation: CAA47892.1.
X61223 Genomic DNA. No translation available.
X61224 Genomic DNA. Translation: CAA43536.1.
AE014134 Genomic DNA. Translation: AAF52304.1.
AE014134 Genomic DNA. Translation: AAN10562.1.
AE014134 Genomic DNA. Translation: AAN10563.1.
AY058492 mRNA. Translation: AAL13721.1.
X80204 Genomic DNA. Translation: CAA56497.1.
M13786 Genomic DNA. Translation: AAA28590.1.
J03927 Genomic DNA. Translation: AAA28553.1.
J03927 Genomic DNA. Translation: AAA28554.1.
J03927 Genomic DNA. Translation: AAA28555.1.
PIRiS06758.
S06759.
S06760.
S21963.
RefSeqiNP_001260111.1. NM_001273182.1. [P13706-4]
NP_001260112.1. NM_001273183.2. [P13706-3]
NP_001260113.1. NM_001273184.2. [P13706-2]
NP_476565.1. NM_057217.4. [P13706-3]
NP_476566.1. NM_057218.4. [P13706-2]
NP_476567.1. NM_057219.4. [P13706-4]
UniGeneiDm.4843.

3D structure databases

ProteinModelPortaliP13706.
SMRiP13706.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi59989. 66 interactors.
DIPiDIP-21689N.
IntActiP13706. 1 interactor.
STRINGi7227.FBpp0305431.

Proteomic databases

PaxDbiP13706.
PRIDEiP13706.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi33824.
KEGGidme:Dmel_CG9042.

Organism-specific databases

CTDi33824.
FlyBaseiFBgn0001128. Gpdh.

Phylogenomic databases

eggNOGiKOG2711. Eukaryota.
COG0240. LUCA.
InParanoidiP13706.
KOiK00006.
OMAiRNHTAGR.
OrthoDBiEOG091G0DO6.
PhylomeDBiP13706.

Enzyme and pathway databases

UniPathwayiUPA00086.
ReactomeiR-DME-1483166. Synthesis of PA.
R-DME-75109. Triglyceride Biosynthesis.

Miscellaneous databases

GenomeRNAii33824.
PROiP13706.

Gene expression databases

BgeeiFBgn0001128.
GenevisibleiP13706. DM.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR017751. G3P_DH_NAD-dep_euk.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTSiPR00077. GPDHDRGNASE.
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR03376. glycerol3P_DH. 1 hit.
PROSITEiPS00957. NAD_G3PDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGPDA_DROME
AccessioniPrimary (citable) accession number: P13706
Secondary accession number(s): Q27590
, Q27925, Q95077, Q95TV4, Q9VML0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 177 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.