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P13706

- GPDA_DROME

UniProt

P13706 - GPDA_DROME

Protein

Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic

Gene

Gpdh

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 160 (01 Oct 2014)
      Sequence version 3 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei98 – 981NADBy similarity
    Binding sitei121 – 1211NAD; via amide nitrogenBy similarity
    Binding sitei121 – 1211SubstrateBy similarity
    Binding sitei155 – 1551NAD; via amide nitrogenBy similarity
    Active sitei206 – 2061Proton acceptorBy similarity
    Binding sitei270 – 2701NADBy similarity
    Binding sitei299 – 2991NADBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi11 – 166NADBy similarity

    GO - Molecular functioni

    1. glycerol-3-phosphate dehydrogenase [NAD+] activity Source: UniProtKB-EC
    2. NAD binding Source: InterPro

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. flight behavior Source: FlyBase
    3. glycerol-3-phosphate catabolic process Source: InterPro
    4. glycerophospholipid metabolic process Source: UniProtKB-UniPathway
    5. triglyceride metabolic process Source: FlyBase

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    ReactomeiREACT_211073. Triglyceride Biosynthesis.
    REACT_220033. Synthesis of PA.
    UniPathwayiUPA00086.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic (EC:1.1.1.8)
    Short name:
    GPD-C
    Short name:
    GPDH-C
    Gene namesi
    Name:Gpdh
    ORF Names:CG9042
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 2L

    Organism-specific databases

    FlyBaseiFBgn0001128. Gpdh.

    Subcellular locationi

    GO - Cellular componenti

    1. glycerol-3-phosphate dehydrogenase complex Source: InterPro
    2. lipid particle Source: FlyBase
    3. M band Source: FlyBase
    4. Z disc Source: FlyBase

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 363363Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmicPRO_0000138075Add
    BLAST

    Proteomic databases

    PaxDbiP13706.

    Expressioni

    Tissue specificityi

    Isoform GPDH-1 is predominant in thorax and isoform GPDH-3 in abdomen.

    Developmental stagei

    Isoform GPDH-2 and isoform GPDH-3 are expressed in both larvae and adults. Isoform GPDH-1 is expressed only in adults.2 Publications

    Gene expression databases

    BgeeiP13706.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    BioGridi59989. 66 interactions.
    DIPiDIP-21689N.

    Structurei

    3D structure databases

    ProteinModelPortaliP13706.
    SMRiP13706. Positions 1-350.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni270 – 2712Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0240.
    InParanoidiP13706.
    KOiK00006.
    OMAiIRIGMME.
    OrthoDBiEOG7ZKSBS.
    PhylomeDBiP13706.

    Family and domain databases

    Gene3Di1.10.1040.10. 1 hit.
    3.40.50.720. 1 hit.
    InterProiIPR008927. 6-PGluconate_DH_C-like.
    IPR013328. DH_multihelical.
    IPR006168. G3P_DH_NAD-dep.
    IPR006109. G3P_DH_NAD-dep_C.
    IPR017751. G3P_DH_NAD-dep_euk.
    IPR011128. G3P_DH_NAD-dep_N.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11728. PTHR11728. 1 hit.
    PfamiPF07479. NAD_Gly3P_dh_C. 1 hit.
    PF01210. NAD_Gly3P_dh_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000114. Glycerol-3-P_dh. 1 hit.
    PRINTSiPR00077. GPDHDRGNASE.
    SUPFAMiSSF48179. SSF48179. 1 hit.
    TIGRFAMsiTIGR03376. glycerol3P_DH. 1 hit.
    PROSITEiPS00957. NAD_G3PDH. 1 hit.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Isoform GPDH-4 (identifier: P13706-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MADKVNVCIV GSGNWGSAIA KIVGANAAAL PEFEERVTMF VYEELIDGKK    50
    LTEIINETHE NVKYLKGHKL PPNVVAVPDL VEAAKNADIL IFVVPHQFIP 100
    NFCKQLLGKI KPNAIAISLI KGFDKAEGGG IDLISHIITR HLKIPCAVLM 150
    GANLANEVAE GNFCETTIGC TDKKYGKVLR DLFQANHFRV VVVDDADAVE 200
    VCGALKNIVA CGAGFVDGLK LGDNTKAAVI RLGLMEMIRF VDVFYPGSKL 250
    STFFESCGVA DLITTCYGGR NRRVSEAFVT SGKTIEELEK EMLNGQKLQG 300
    PPTAEEVNYM LKNKGLEDKF PLFTAIHKIC TNQLKPNDLI DCIRNHPEHM 350
    DTSIMPSPKL QNL 363
    Length:363
    Mass (Da):39,684
    Last modified:June 1, 2001 - v3
    Checksum:i49FA09CE7FB6FDF4
    GO
    Isoform GPDH-1 (identifier: P13706-2) [UniParc]FASTAAdd to Basket

    Also known as: GPDH-411, C

    The sequence of this isoform differs from the canonical sequence as follows:
         351-360: Missing.

    Show »
    Length:353
    Mass (Da):38,614
    Checksum:i629616247F906ACF
    GO
    Isoform GPDH-2 (identifier: P13706-3) [UniParc]FASTAAdd to Basket

    Also known as: GPDH-37, B

    The sequence of this isoform differs from the canonical sequence as follows:
         361-363: Missing.

    Show »
    Length:360
    Mass (Da):39,329
    Checksum:i7E7FB6FDF432CDD0
    GO
    Isoform GPDH-3 (identifier: P13706-4) [UniParc]FASTAAdd to Basket

    Also known as: GPDH-1A, A

    The sequence of this isoform differs from the canonical sequence as follows:
         351-363: Missing.

    Show »
    Length:350
    Mass (Da):38,259
    Checksum:iB47F906ACF061F8E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti351 – 36313DTSIM…KLQNL → GYVHHAVAKTCKICKSPKEA ETFLTFLCTTENKCIFSAQR QLDESLDSIF in CAA47892. (PubMed:8094564)CuratedAdd
    BLAST

    Polymorphismi

    There are two common alleles; fast and slow. The sequence of fast is shown here.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti14 – 141N → Y in allele GPDH-ACYG22.
    Natural varianti266 – 2661C → G in allele GPDH-ACB62.
    Natural varianti273 – 2731R → C in allele GPDH-ACYG22.
    Natural varianti337 – 3371N → K in allele GPDH-AT198, allele GPDH-ACB62 and allele GPDH-S.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei351 – 36313Missing in isoform GPDH-3. CuratedVSP_001590Add
    BLAST
    Alternative sequencei351 – 36010Missing in isoform GPDH-1. CuratedVSP_001589
    Alternative sequencei361 – 3633Missing in isoform GPDH-2. 1 PublicationVSP_001591

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04567 Genomic DNA. Translation: AAA28591.1.
    J04567 Genomic DNA. Translation: AAA28592.1.
    J04567 Genomic DNA. Translation: AAA28593.1.
    X14179 Genomic DNA. Translation: CAA32379.1.
    X14179 Genomic DNA. Translation: CAA32380.1.
    X14179 Genomic DNA. Translation: CAA32381.1.
    X67650 Genomic DNA. Translation: CAA47892.1.
    X61223 Genomic DNA. No translation available.
    X61224 Genomic DNA. Translation: CAA43536.1.
    AE014134 Genomic DNA. Translation: AAF52304.1.
    AE014134 Genomic DNA. Translation: AAN10562.1.
    AE014134 Genomic DNA. Translation: AAN10563.1.
    AY058492 mRNA. Translation: AAL13721.1.
    X80204 Genomic DNA. Translation: CAA56497.1.
    M13786 Genomic DNA. Translation: AAA28590.1.
    J03927 Genomic DNA. Translation: AAA28553.1.
    J03927 Genomic DNA. Translation: AAA28554.1.
    J03927 Genomic DNA. Translation: AAA28555.1.
    PIRiS06758.
    S06759.
    S06760.
    S21963.
    RefSeqiNP_001260111.1. NM_001273182.1. [P13706-4]
    NP_001260112.1. NM_001273183.1. [P13706-3]
    NP_001260113.1. NM_001273184.1. [P13706-2]
    NP_476565.1. NM_057217.4. [P13706-3]
    NP_476566.1. NM_057218.4. [P13706-2]
    NP_476567.1. NM_057219.4. [P13706-4]
    UniGeneiDm.4843.

    Genome annotation databases

    GeneIDi33824.
    KEGGidme:Dmel_CG9042.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04567 Genomic DNA. Translation: AAA28591.1 .
    J04567 Genomic DNA. Translation: AAA28592.1 .
    J04567 Genomic DNA. Translation: AAA28593.1 .
    X14179 Genomic DNA. Translation: CAA32379.1 .
    X14179 Genomic DNA. Translation: CAA32380.1 .
    X14179 Genomic DNA. Translation: CAA32381.1 .
    X67650 Genomic DNA. Translation: CAA47892.1 .
    X61223 Genomic DNA. No translation available.
    X61224 Genomic DNA. Translation: CAA43536.1 .
    AE014134 Genomic DNA. Translation: AAF52304.1 .
    AE014134 Genomic DNA. Translation: AAN10562.1 .
    AE014134 Genomic DNA. Translation: AAN10563.1 .
    AY058492 mRNA. Translation: AAL13721.1 .
    X80204 Genomic DNA. Translation: CAA56497.1 .
    M13786 Genomic DNA. Translation: AAA28590.1 .
    J03927 Genomic DNA. Translation: AAA28553.1 .
    J03927 Genomic DNA. Translation: AAA28554.1 .
    J03927 Genomic DNA. Translation: AAA28555.1 .
    PIRi S06758.
    S06759.
    S06760.
    S21963.
    RefSeqi NP_001260111.1. NM_001273182.1. [P13706-4 ]
    NP_001260112.1. NM_001273183.1. [P13706-3 ]
    NP_001260113.1. NM_001273184.1. [P13706-2 ]
    NP_476565.1. NM_057217.4. [P13706-3 ]
    NP_476566.1. NM_057218.4. [P13706-2 ]
    NP_476567.1. NM_057219.4. [P13706-4 ]
    UniGenei Dm.4843.

    3D structure databases

    ProteinModelPortali P13706.
    SMRi P13706. Positions 1-350.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 59989. 66 interactions.
    DIPi DIP-21689N.

    Proteomic databases

    PaxDbi P13706.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 33824.
    KEGGi dme:Dmel_CG9042.

    Organism-specific databases

    CTDi 33824.
    FlyBasei FBgn0001128. Gpdh.

    Phylogenomic databases

    eggNOGi COG0240.
    InParanoidi P13706.
    KOi K00006.
    OMAi IRIGMME.
    OrthoDBi EOG7ZKSBS.
    PhylomeDBi P13706.

    Enzyme and pathway databases

    UniPathwayi UPA00086 .
    Reactomei REACT_211073. Triglyceride Biosynthesis.
    REACT_220033. Synthesis of PA.

    Miscellaneous databases

    GenomeRNAii 33824.
    NextBioi 785441.
    PROi P13706.

    Gene expression databases

    Bgeei P13706.

    Family and domain databases

    Gene3Di 1.10.1040.10. 1 hit.
    3.40.50.720. 1 hit.
    InterProi IPR008927. 6-PGluconate_DH_C-like.
    IPR013328. DH_multihelical.
    IPR006168. G3P_DH_NAD-dep.
    IPR006109. G3P_DH_NAD-dep_C.
    IPR017751. G3P_DH_NAD-dep_euk.
    IPR011128. G3P_DH_NAD-dep_N.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11728. PTHR11728. 1 hit.
    Pfami PF07479. NAD_Gly3P_dh_C. 1 hit.
    PF01210. NAD_Gly3P_dh_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000114. Glycerol-3-P_dh. 1 hit.
    PRINTSi PR00077. GPDHDRGNASE.
    SUPFAMi SSF48179. SSF48179. 1 hit.
    TIGRFAMsi TIGR03376. glycerol3P_DH. 1 hit.
    PROSITEi PS00957. NAD_G3PDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural characterization of the alpha-glycerol-3-phosphate dehydrogenase-encoding gene of Drosophila melanogaster."
      von Kalm L., Weaver J., Demarco J., Macintyre R.J., Sullivan D.T.
      Proc. Natl. Acad. Sci. U.S.A. 86:5020-5024(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING, DEVELOPMENTAL STAGE.
      Strain: Canton-S.
    2. "Sequence, structure and evolution of the gene coding for sn-glycerol-3-phosphate dehydrogenase in Drosophila melanogaster."
      Bewley G.C., Cook J.L., Kusakabe S., Mukai T., Rigby D.L., Chambers G.K.
      Nucleic Acids Res. 17:8553-8567(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 21-133; 139-141 AND 148-350, ALTERNATIVE SPLICING, DEFINITION OF ALLELES FAST AND SLOW.
      Strain: Canton-S, Ogasawara and Oregon-R.
    3. "Defective P element insertions affect the expression of sn-glycerol-3-phosphate dehydrogenase alleles in natural populations of Drosophila melanogaster."
      Reed D.S., Gibson J.B.
      Proc. R. Soc. B 251:39-45(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTANTS.
      Strain: Tasmania.
    4. "Molecular analysis of a Drosophila melanogaster sn-glycerol-3-phosphate dehydrogenase allozyme variant that has cold labile activity."
      Symonds J.E., Gibson J.B., Wilks A.V., Wilanowski T.M.
      Insect Biochem. Mol. Biol. 25:789-798(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ALLELE GPDH-ACB62.
      Strain: Cardwell.
    5. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    6. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
      Strain: Berkeley.
    7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM GPDH-2).
      Strain: Berkeley.
      Tissue: Ovary.
    8. "Molecular heterogeneity of naturally occurring sn-glycerol-3-phosphate dehydrogenase low-activity variants in Drosophila melanogaster."
      Reed D.S., Gibson J.B.
      Biochem. Genet. 32:161-179(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 2-363, VARIANTS ALLELES GPDH-MB5 AND GPDH-ACYG22.
      Strain: Cygnet and Mission beach.
    9. "Isolation of a genomic clone for Drosophila sn-glycerol-3-phosphate dehydrogenase using synthetic oligonucleotides."
      Cook J.L., Shaffer J.B., Bewley G.C., MacIntyre R.J., Wright D.A.
      J. Biol. Chem. 261:11751-11755(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 206-245.
      Strain: Canton-S.
    10. "Drosophila sn-glycerol-3-phosphate dehydrogenase isozymes are generated by alternate pathways of RNA processing resulting in different carboxyl-terminal amino acid sequences."
      Cook J.L., Bewley G.C., Shaffer J.B.
      J. Biol. Chem. 263:10858-10864(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 298-350, ALTERNATIVE SPLICING, DEVELOPMENTAL STAGE.
      Strain: Canton-S.

    Entry informationi

    Entry nameiGPDA_DROME
    AccessioniPrimary (citable) accession number: P13706
    Secondary accession number(s): Q27590
    , Q27925, Q95077, Q95TV4, Q9VML0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 160 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3