SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P13706

- GPDA_DROME

UniProt

P13706 - GPDA_DROME

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic

Gene
Gpdh, CG9042
Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei98 – 981NAD By similarity
Binding sitei121 – 1211NAD; via amide nitrogen By similarity
Binding sitei121 – 1211Substrate By similarity
Binding sitei155 – 1551NAD; via amide nitrogen By similarity
Active sitei206 – 2061Proton acceptor By similarity
Binding sitei270 – 2701NAD By similarity
Binding sitei299 – 2991NAD By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 166NAD By similarity

GO - Molecular functioni

  1. glycerol-3-phosphate dehydrogenase [NAD+] activity Source: UniProtKB-EC
  2. NAD binding Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. flight behavior Source: FlyBase
  3. glycerol-3-phosphate catabolic process Source: InterPro
  4. glycerophospholipid metabolic process Source: UniProtKB-UniPathway
  5. triglyceride metabolic process Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiREACT_211073. Triglyceride Biosynthesis.
REACT_220033. Synthesis of PA.
UniPathwayiUPA00086.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic (EC:1.1.1.8)
Short name:
GPD-C
Short name:
GPDH-C
Gene namesi
Name:Gpdh
ORF Names:CG9042
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0001128. Gpdh.

Subcellular locationi

GO - Cellular componenti

  1. glycerol-3-phosphate dehydrogenase complex Source: InterPro
  2. lipid particle Source: FlyBase
  3. M band Source: FlyBase
  4. Z disc Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 363363Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmicPRO_0000138075Add
BLAST

Proteomic databases

PaxDbiP13706.

Expressioni

Tissue specificityi

Isoform GPDH-1 is predominant in thorax and isoform GPDH-3 in abdomen.

Developmental stagei

Isoform GPDH-2 and isoform GPDH-3 are expressed in both larvae and adults. Isoform GPDH-1 is expressed only in adults.2 Publications

Gene expression databases

BgeeiP13706.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi59989. 66 interactions.
DIPiDIP-21689N.

Structurei

3D structure databases

ProteinModelPortaliP13706.
SMRiP13706. Positions 1-350.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni270 – 2712Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0240.
InParanoidiP13706.
KOiK00006.
OMAiIRIGMME.
OrthoDBiEOG7ZKSBS.
PhylomeDBiP13706.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. DH_multihelical.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR017751. G3P_DH_NAD-dep_euk.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11728. PTHR11728. 1 hit.
PfamiPF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTSiPR00077. GPDHDRGNASE.
SUPFAMiSSF48179. SSF48179. 1 hit.
TIGRFAMsiTIGR03376. glycerol3P_DH. 1 hit.
PROSITEiPS00957. NAD_G3PDH. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. Align

Isoform GPDH-4 (identifier: P13706-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MADKVNVCIV GSGNWGSAIA KIVGANAAAL PEFEERVTMF VYEELIDGKK    50
LTEIINETHE NVKYLKGHKL PPNVVAVPDL VEAAKNADIL IFVVPHQFIP 100
NFCKQLLGKI KPNAIAISLI KGFDKAEGGG IDLISHIITR HLKIPCAVLM 150
GANLANEVAE GNFCETTIGC TDKKYGKVLR DLFQANHFRV VVVDDADAVE 200
VCGALKNIVA CGAGFVDGLK LGDNTKAAVI RLGLMEMIRF VDVFYPGSKL 250
STFFESCGVA DLITTCYGGR NRRVSEAFVT SGKTIEELEK EMLNGQKLQG 300
PPTAEEVNYM LKNKGLEDKF PLFTAIHKIC TNQLKPNDLI DCIRNHPEHM 350
DTSIMPSPKL QNL 363
Length:363
Mass (Da):39,684
Last modified:June 1, 2001 - v3
Checksum:i49FA09CE7FB6FDF4
GO
Isoform GPDH-1 (identifier: P13706-2) [UniParc]FASTAAdd to Basket

Also known as: GPDH-411, C

The sequence of this isoform differs from the canonical sequence as follows:
     351-360: Missing.

Show »
Length:353
Mass (Da):38,614
Checksum:i629616247F906ACF
GO
Isoform GPDH-2 (identifier: P13706-3) [UniParc]FASTAAdd to Basket

Also known as: GPDH-37, B

The sequence of this isoform differs from the canonical sequence as follows:
     361-363: Missing.

Show »
Length:360
Mass (Da):39,329
Checksum:i7E7FB6FDF432CDD0
GO
Isoform GPDH-3 (identifier: P13706-4) [UniParc]FASTAAdd to Basket

Also known as: GPDH-1A, A

The sequence of this isoform differs from the canonical sequence as follows:
     351-363: Missing.

Show »
Length:350
Mass (Da):38,259
Checksum:iB47F906ACF061F8E
GO

Polymorphismi

There are two common alleles; fast and slow. The sequence of fast is shown here.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti14 – 141N → Y in allele GPDH-ACYG22.
Natural varianti266 – 2661C → G in allele GPDH-ACB62.
Natural varianti273 – 2731R → C in allele GPDH-ACYG22.
Natural varianti337 – 3371N → K in allele GPDH-AT198, allele GPDH-ACB62 and allele GPDH-S.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei351 – 36313Missing in isoform GPDH-3. VSP_001590Add
BLAST
Alternative sequencei351 – 36010Missing in isoform GPDH-1. VSP_001589
Alternative sequencei361 – 3633Missing in isoform GPDH-2. VSP_001591

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti351 – 36313DTSIM…KLQNL → GYVHHAVAKTCKICKSPKEA ETFLTFLCTTENKCIFSAQR QLDESLDSIF in CAA47892. 1 PublicationAdd
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J04567 Genomic DNA. Translation: AAA28591.1.
J04567 Genomic DNA. Translation: AAA28592.1.
J04567 Genomic DNA. Translation: AAA28593.1.
X14179 Genomic DNA. Translation: CAA32379.1.
X14179 Genomic DNA. Translation: CAA32380.1.
X14179 Genomic DNA. Translation: CAA32381.1.
X67650 Genomic DNA. Translation: CAA47892.1.
X61223 Genomic DNA. No translation available.
X61224 Genomic DNA. Translation: CAA43536.1.
AE014134 Genomic DNA. Translation: AAF52304.1.
AE014134 Genomic DNA. Translation: AAN10562.1.
AE014134 Genomic DNA. Translation: AAN10563.1.
AY058492 mRNA. Translation: AAL13721.1.
X80204 Genomic DNA. Translation: CAA56497.1.
M13786 Genomic DNA. Translation: AAA28590.1.
J03927 Genomic DNA. Translation: AAA28553.1.
J03927 Genomic DNA. Translation: AAA28554.1.
J03927 Genomic DNA. Translation: AAA28555.1.
PIRiS06758.
S06759.
S06760.
S21963.
RefSeqiNP_001260111.1. NM_001273182.1. [P13706-4]
NP_001260112.1. NM_001273183.1. [P13706-3]
NP_001260113.1. NM_001273184.1. [P13706-2]
NP_476565.1. NM_057217.4. [P13706-3]
NP_476566.1. NM_057218.4. [P13706-2]
NP_476567.1. NM_057219.4. [P13706-4]
UniGeneiDm.4843.

Genome annotation databases

GeneIDi33824.
KEGGidme:Dmel_CG9042.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J04567 Genomic DNA. Translation: AAA28591.1 .
J04567 Genomic DNA. Translation: AAA28592.1 .
J04567 Genomic DNA. Translation: AAA28593.1 .
X14179 Genomic DNA. Translation: CAA32379.1 .
X14179 Genomic DNA. Translation: CAA32380.1 .
X14179 Genomic DNA. Translation: CAA32381.1 .
X67650 Genomic DNA. Translation: CAA47892.1 .
X61223 Genomic DNA. No translation available.
X61224 Genomic DNA. Translation: CAA43536.1 .
AE014134 Genomic DNA. Translation: AAF52304.1 .
AE014134 Genomic DNA. Translation: AAN10562.1 .
AE014134 Genomic DNA. Translation: AAN10563.1 .
AY058492 mRNA. Translation: AAL13721.1 .
X80204 Genomic DNA. Translation: CAA56497.1 .
M13786 Genomic DNA. Translation: AAA28590.1 .
J03927 Genomic DNA. Translation: AAA28553.1 .
J03927 Genomic DNA. Translation: AAA28554.1 .
J03927 Genomic DNA. Translation: AAA28555.1 .
PIRi S06758.
S06759.
S06760.
S21963.
RefSeqi NP_001260111.1. NM_001273182.1. [P13706-4 ]
NP_001260112.1. NM_001273183.1. [P13706-3 ]
NP_001260113.1. NM_001273184.1. [P13706-2 ]
NP_476565.1. NM_057217.4. [P13706-3 ]
NP_476566.1. NM_057218.4. [P13706-2 ]
NP_476567.1. NM_057219.4. [P13706-4 ]
UniGenei Dm.4843.

3D structure databases

ProteinModelPortali P13706.
SMRi P13706. Positions 1-350.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 59989. 66 interactions.
DIPi DIP-21689N.

Proteomic databases

PaxDbi P13706.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 33824.
KEGGi dme:Dmel_CG9042.

Organism-specific databases

CTDi 33824.
FlyBasei FBgn0001128. Gpdh.

Phylogenomic databases

eggNOGi COG0240.
InParanoidi P13706.
KOi K00006.
OMAi IRIGMME.
OrthoDBi EOG7ZKSBS.
PhylomeDBi P13706.

Enzyme and pathway databases

UniPathwayi UPA00086 .
Reactomei REACT_211073. Triglyceride Biosynthesis.
REACT_220033. Synthesis of PA.

Miscellaneous databases

GenomeRNAii 33824.
NextBioi 785441.
PROi P13706.

Gene expression databases

Bgeei P13706.

Family and domain databases

Gene3Di 1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProi IPR008927. 6-PGluconate_DH_C-like.
IPR013328. DH_multihelical.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR017751. G3P_DH_NAD-dep_euk.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR11728. PTHR11728. 1 hit.
Pfami PF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTSi PR00077. GPDHDRGNASE.
SUPFAMi SSF48179. SSF48179. 1 hit.
TIGRFAMsi TIGR03376. glycerol3P_DH. 1 hit.
PROSITEi PS00957. NAD_G3PDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural characterization of the alpha-glycerol-3-phosphate dehydrogenase-encoding gene of Drosophila melanogaster."
    von Kalm L., Weaver J., Demarco J., Macintyre R.J., Sullivan D.T.
    Proc. Natl. Acad. Sci. U.S.A. 86:5020-5024(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING, DEVELOPMENTAL STAGE.
    Strain: Canton-S.
  2. "Sequence, structure and evolution of the gene coding for sn-glycerol-3-phosphate dehydrogenase in Drosophila melanogaster."
    Bewley G.C., Cook J.L., Kusakabe S., Mukai T., Rigby D.L., Chambers G.K.
    Nucleic Acids Res. 17:8553-8567(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 21-133; 139-141 AND 148-350, ALTERNATIVE SPLICING, DEFINITION OF ALLELES FAST AND SLOW.
    Strain: Canton-S, Ogasawara and Oregon-R.
  3. "Defective P element insertions affect the expression of sn-glycerol-3-phosphate dehydrogenase alleles in natural populations of Drosophila melanogaster."
    Reed D.S., Gibson J.B.
    Proc. R. Soc. B 251:39-45(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTANTS.
    Strain: Tasmania.
  4. "Molecular analysis of a Drosophila melanogaster sn-glycerol-3-phosphate dehydrogenase allozyme variant that has cold labile activity."
    Symonds J.E., Gibson J.B., Wilks A.V., Wilanowski T.M.
    Insect Biochem. Mol. Biol. 25:789-798(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ALLELE GPDH-ACB62.
    Strain: Cardwell.
  5. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  6. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM GPDH-2).
    Strain: Berkeley.
    Tissue: Ovary.
  8. "Molecular heterogeneity of naturally occurring sn-glycerol-3-phosphate dehydrogenase low-activity variants in Drosophila melanogaster."
    Reed D.S., Gibson J.B.
    Biochem. Genet. 32:161-179(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 2-363, VARIANTS ALLELES GPDH-MB5 AND GPDH-ACYG22.
    Strain: Cygnet and Mission beach.
  9. "Isolation of a genomic clone for Drosophila sn-glycerol-3-phosphate dehydrogenase using synthetic oligonucleotides."
    Cook J.L., Shaffer J.B., Bewley G.C., MacIntyre R.J., Wright D.A.
    J. Biol. Chem. 261:11751-11755(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 206-245.
    Strain: Canton-S.
  10. "Drosophila sn-glycerol-3-phosphate dehydrogenase isozymes are generated by alternate pathways of RNA processing resulting in different carboxyl-terminal amino acid sequences."
    Cook J.L., Bewley G.C., Shaffer J.B.
    J. Biol. Chem. 263:10858-10864(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 298-350, ALTERNATIVE SPLICING, DEVELOPMENTAL STAGE.
    Strain: Canton-S.

Entry informationi

Entry nameiGPDA_DROME
AccessioniPrimary (citable) accession number: P13706
Secondary accession number(s): Q27590
, Q27925, Q95077, Q95TV4, Q9VML0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: June 1, 2001
Last modified: September 3, 2014
This is version 159 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi