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P13706 (GPDA_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 158. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic

Short name=GPD-C
Short name=GPDH-C
EC=1.1.1.8
Gene names
Name:Gpdh
ORF Names:CG9042
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length363 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.

Pathway

Phospholipid metabolism; alpha-glycerophosphate cycle.

Subunit structure

Homodimer.

Subcellular location

Cytoplasm.

Tissue specificity

Isoform GPDH-1 is predominant in thorax and isoform GPDH-3 in abdomen.

Developmental stage

Isoform GPDH-2 and isoform GPDH-3 are expressed in both larvae and adults. Isoform GPDH-1 is expressed only in adults. Ref.1 Ref.10

Polymorphism

There are two common alleles; fast and slow. The sequence of fast is shown here.

Sequence similarities

Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform GPDH-4 (identifier: P13706-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform GPDH-1 (identifier: P13706-2)

Also known as: GPDH-411; C;

The sequence of this isoform differs from the canonical sequence as follows:
     351-360: Missing.
Isoform GPDH-2 (identifier: P13706-3)

Also known as: GPDH-37; B;

The sequence of this isoform differs from the canonical sequence as follows:
     361-363: Missing.
Isoform GPDH-3 (identifier: P13706-4)

Also known as: GPDH-1A; A;

The sequence of this isoform differs from the canonical sequence as follows:
     351-363: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 363363Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic
PRO_0000138075

Regions

Nucleotide binding11 – 166NAD By similarity
Region270 – 2712Substrate binding By similarity

Sites

Active site2061Proton acceptor By similarity
Binding site981NAD By similarity
Binding site1211NAD; via amide nitrogen By similarity
Binding site1211Substrate By similarity
Binding site1551NAD; via amide nitrogen By similarity
Binding site2701NAD By similarity
Binding site2991NAD By similarity

Natural variations

Alternative sequence351 – 36313Missing in isoform GPDH-3.
VSP_001590
Alternative sequence351 – 36010Missing in isoform GPDH-1.
VSP_001589
Alternative sequence361 – 3633Missing in isoform GPDH-2.
VSP_001591
Natural variant141N → Y in allele GPDH-ACYG22.
Natural variant2661C → G in allele GPDH-ACB62.
Natural variant2731R → C in allele GPDH-ACYG22.
Natural variant3371N → K in allele GPDH-AT198, allele GPDH-ACB62 and allele GPDH-S.

Experimental info

Sequence conflict351 – 36313DTSIM…KLQNL → GYVHHAVAKTCKICKSPKEA ETFLTFLCTTENKCIFSAQR QLDESLDSIF in CAA47892. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform GPDH-4 [UniParc].

Last modified June 1, 2001. Version 3.
Checksum: 49FA09CE7FB6FDF4

FASTA36339,684
        10         20         30         40         50         60 
MADKVNVCIV GSGNWGSAIA KIVGANAAAL PEFEERVTMF VYEELIDGKK LTEIINETHE 

        70         80         90        100        110        120 
NVKYLKGHKL PPNVVAVPDL VEAAKNADIL IFVVPHQFIP NFCKQLLGKI KPNAIAISLI 

       130        140        150        160        170        180 
KGFDKAEGGG IDLISHIITR HLKIPCAVLM GANLANEVAE GNFCETTIGC TDKKYGKVLR 

       190        200        210        220        230        240 
DLFQANHFRV VVVDDADAVE VCGALKNIVA CGAGFVDGLK LGDNTKAAVI RLGLMEMIRF 

       250        260        270        280        290        300 
VDVFYPGSKL STFFESCGVA DLITTCYGGR NRRVSEAFVT SGKTIEELEK EMLNGQKLQG 

       310        320        330        340        350        360 
PPTAEEVNYM LKNKGLEDKF PLFTAIHKIC TNQLKPNDLI DCIRNHPEHM DTSIMPSPKL 


QNL 

« Hide

Isoform GPDH-1 (GPDH-411) (C) [UniParc].

Checksum: 629616247F906ACF
Show »

FASTA35338,614
Isoform GPDH-2 (GPDH-37) (B) [UniParc].

Checksum: 7E7FB6FDF432CDD0
Show »

FASTA36039,329
Isoform GPDH-3 (GPDH-1A) (A) [UniParc].

Checksum: B47F906ACF061F8E
Show »

FASTA35038,259

References

« Hide 'large scale' references
[1]"Structural characterization of the alpha-glycerol-3-phosphate dehydrogenase-encoding gene of Drosophila melanogaster."
von Kalm L., Weaver J., Demarco J., Macintyre R.J., Sullivan D.T.
Proc. Natl. Acad. Sci. U.S.A. 86:5020-5024(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING, DEVELOPMENTAL STAGE.
Strain: Canton-S.
[2]"Sequence, structure and evolution of the gene coding for sn-glycerol-3-phosphate dehydrogenase in Drosophila melanogaster."
Bewley G.C., Cook J.L., Kusakabe S., Mukai T., Rigby D.L., Chambers G.K.
Nucleic Acids Res. 17:8553-8567(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 21-133; 139-141 AND 148-350, ALTERNATIVE SPLICING, DEFINITION OF ALLELES FAST AND SLOW.
Strain: Canton-S, Ogasawara and Oregon-R.
[3]"Defective P element insertions affect the expression of sn-glycerol-3-phosphate dehydrogenase alleles in natural populations of Drosophila melanogaster."
Reed D.S., Gibson J.B.
Proc. R. Soc. B 251:39-45(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTANTS.
Strain: Tasmania.
[4]"Molecular analysis of a Drosophila melanogaster sn-glycerol-3-phosphate dehydrogenase allozyme variant that has cold labile activity."
Symonds J.E., Gibson J.B., Wilks A.V., Wilanowski T.M.
Insect Biochem. Mol. Biol. 25:789-798(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ALLELE GPDH-ACB62.
Strain: Cardwell.
[5]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[6]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
Strain: Berkeley.
[7]"A Drosophila full-length cDNA resource."
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM GPDH-2).
Strain: Berkeley.
Tissue: Ovary.
[8]"Molecular heterogeneity of naturally occurring sn-glycerol-3-phosphate dehydrogenase low-activity variants in Drosophila melanogaster."
Reed D.S., Gibson J.B.
Biochem. Genet. 32:161-179(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 2-363, VARIANTS ALLELES GPDH-MB5 AND GPDH-ACYG22.
Strain: Cygnet and Mission beach.
[9]"Isolation of a genomic clone for Drosophila sn-glycerol-3-phosphate dehydrogenase using synthetic oligonucleotides."
Cook J.L., Shaffer J.B., Bewley G.C., MacIntyre R.J., Wright D.A.
J. Biol. Chem. 261:11751-11755(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 206-245.
Strain: Canton-S.
[10]"Drosophila sn-glycerol-3-phosphate dehydrogenase isozymes are generated by alternate pathways of RNA processing resulting in different carboxyl-terminal amino acid sequences."
Cook J.L., Bewley G.C., Shaffer J.B.
J. Biol. Chem. 263:10858-10864(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 298-350, ALTERNATIVE SPLICING, DEVELOPMENTAL STAGE.
Strain: Canton-S.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J04567 Genomic DNA. Translation: AAA28591.1.
J04567 Genomic DNA. Translation: AAA28592.1.
J04567 Genomic DNA. Translation: AAA28593.1.
X14179 Genomic DNA. Translation: CAA32379.1.
X14179 Genomic DNA. Translation: CAA32380.1.
X14179 Genomic DNA. Translation: CAA32381.1.
X67650 Genomic DNA. Translation: CAA47892.1.
X61223 Genomic DNA. No translation available.
X61224 Genomic DNA. Translation: CAA43536.1.
AE014134 Genomic DNA. Translation: AAF52304.1.
AE014134 Genomic DNA. Translation: AAN10562.1.
AE014134 Genomic DNA. Translation: AAN10563.1.
AY058492 mRNA. Translation: AAL13721.1.
X80204 Genomic DNA. Translation: CAA56497.1.
M13786 Genomic DNA. Translation: AAA28590.1.
J03927 Genomic DNA. Translation: AAA28553.1.
J03927 Genomic DNA. Translation: AAA28554.1.
J03927 Genomic DNA. Translation: AAA28555.1.
PIRS06758.
S06759.
S06760.
S21963.
RefSeqNP_001260111.1. NM_001273182.1. [P13706-4]
NP_001260112.1. NM_001273183.1. [P13706-3]
NP_001260113.1. NM_001273184.1. [P13706-2]
NP_476565.1. NM_057217.4. [P13706-3]
NP_476566.1. NM_057218.4. [P13706-2]
NP_476567.1. NM_057219.4. [P13706-4]
UniGeneDm.4843.

3D structure databases

ProteinModelPortalP13706.
SMRP13706. Positions 1-350.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid59989. 66 interactions.
DIPDIP-21689N.

Proteomic databases

PaxDbP13706.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID33824.
KEGGdme:Dmel_CG9042.

Organism-specific databases

CTD33824.
FlyBaseFBgn0001128. Gpdh.

Phylogenomic databases

eggNOGCOG0240.
InParanoidP13706.
KOK00006.
OMAIRIGMME.
OrthoDBEOG7ZKSBS.
PhylomeDBP13706.

Enzyme and pathway databases

UniPathwayUPA00086.

Gene expression databases

BgeeP13706.

Family and domain databases

Gene3D1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProIPR008927. 6-PGluconate_DH_C-like.
IPR013328. DH_multihelical.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR017751. G3P_DH_NAD-dep_euk.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR11728. PTHR11728. 1 hit.
PfamPF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view]
PIRSFPIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTSPR00077. GPDHDRGNASE.
SUPFAMSSF48179. SSF48179. 1 hit.
TIGRFAMsTIGR03376. glycerol3P_DH. 1 hit.
PROSITEPS00957. NAD_G3PDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi33824.
NextBio785441.
PROP13706.

Entry information

Entry nameGPDA_DROME
AccessionPrimary (citable) accession number: P13706
Secondary accession number(s): Q27590 expand/collapse secondary AC list , Q27925, Q95077, Q95TV4, Q9VML0
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 158 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase