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Protein

DNA mismatch repair protein Msh3

Gene

Msh3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi850 – 8578ATPSequence analysis

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • centromeric DNA binding Source: MGI
  • damaged DNA binding Source: MGI
  • DNA binding Source: MGI
  • enzyme binding Source: MGI
  • mismatched DNA binding Source: InterPro

GO - Biological processi

  • DNA repair Source: MGI
  • maintenance of DNA repeat elements Source: MGI
  • mismatch repair Source: MGI
  • negative regulation of DNA recombination Source: MGI
  • positive regulation of helicase activity Source: MGI
  • somatic recombination of immunoglobulin gene segments Source: MGI
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA mismatch repair protein Msh3
Alternative name(s):
Protein repair-1
Short name:
REP-1
Protein repair-3
Short name:
REP-3
Gene namesi
Name:Msh3
Synonyms:Rep-3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:109519. Msh3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10911091DNA mismatch repair protein Msh3PRO_0000115193Add
BLAST

Proteomic databases

EPDiP13705.
MaxQBiP13705.
PaxDbiP13705.
PRIDEiP13705.

PTM databases

iPTMnetiP13705.
PhosphoSiteiP13705.

Expressioni

Gene expression databases

CleanExiMM_MSH3.

Interactioni

Subunit structurei

Heterodimer consisting of MSH2-MSH3 (MutS beta). Forms a ternary complex with MutL alpha (MLH1-PMS1). Interacts with EXO1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201526. 1 interaction.
STRINGi10090.ENSMUSP00000022220.

Structurei

3D structure databases

ProteinModelPortaliP13705.
SMRiP13705. Positions 183-1076.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0217. Eukaryota.
COG0249. LUCA.
HOVERGENiHBG006400.
InParanoidiP13705.
KOiK08736.
PhylomeDBiP13705.

Family and domain databases

Gene3Di3.30.420.110. 1 hit.
3.40.1170.10. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR007695. DNA_mismatch_repair_MutS-lik_N.
IPR000432. DNA_mismatch_repair_MutS_C.
IPR007696. DNA_mismatch_repair_MutS_core.
IPR016151. DNA_mismatch_repair_MutS_N.
IPR007860. DNA_mmatch_repair_MutS_con_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01624. MutS_I. 1 hit.
PF05188. MutS_II. 1 hit.
PF05192. MutS_III. 1 hit.
PF00488. MutS_V. 1 hit.
[Graphical view]
SMARTiSM00534. MUTSac. 1 hit.
SM00533. MUTSd. 1 hit.
[Graphical view]
SUPFAMiSSF48334. SSF48334. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF53150. SSF53150. 2 hits.
SSF55271. SSF55271. 1 hit.
PROSITEiPS00486. DNA_MISMATCH_REPAIR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P13705-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRGKSASGG STAAGPGPGR QTVLSRFFRS AGSLRSSASS TEPAEKVTEG
60 70 80 90 100
DSRKRSLGNG GPTKKKARKV PEKEEENISV ASHHPEAKKC LRPRIVLKSL
110 120 130 140 150
EKLKEFCCDS ALPQNRVQTE ALRERLEVLP RCTDFEDITL QRAKNAVLSE
160 170 180 190 200
DSKSQANQKD SQFGPCPEVF QKTSDCKPFN KRSKSVYTPL ELQYLDMKQQ
210 220 230 240 250
HKDAVLCVEC GYKYRFFGED AEIAARELNI YCHLDHNFMT ASIPTHRLFV
260 270 280 290 300
HVRRLVAKGY KVGVVKQTET AALKAIGDNK SSVFSRKLTA LYTKSTLIGE
310 320 330 340 350
DVNPLIRLDD SVNIDEVMTD TSTNYLLCIY EEKENIKDKK KGNLSVGVVG
360 370 380 390 400
VQPATGEVVF DCFQDSASRL ELETRISSLQ PVELLLPSDL SEPTEMLIQR
410 420 430 440 450
ATNVSVRDDR IRVERMNNTY FEYSHAFQTV TEFYAREIVD SQGSQSLSGV
460 470 480 490 500
INLEKPVICA LAAVIRYLKE FNLEKMLSKP ESFKQLSSGM EFMRINGTTL
510 520 530 540 550
RNLEMVQNQT DMKTKGSLLW VLDHTKTSFG RRKLKNWVTQ PLLKLREINA
560 570 580 590 600
RLDAVSDVLH SESSVFEQIE NLLRKLPDVE RGLCSIYHKK CSTQEFFLIV
610 620 630 640 650
KSLCQLKSEL QALMPAVNSH VQSDLLRALI VELLSPVEHY LKVLNGPAAK
660 670 680 690 700
VGDKTELFKD LSDFPLIKKR KNEIQEVIHS IQMRLQEFRK ILKLPSLQYV
710 720 730 740 750
TVSGQEFMIE IKNSAVSCIP ADWVKVGSTK AVSRFHPPFI VESYRRLNQL
760 770 780 790 800
REQLVLDCNA EWLGFLENFG EHYHTLCKAV DHLATVDCIF SLAKVAKQGN
810 820 830 840 850
YCRPTLQEEK KIIIKNGRHP MIDVLLGEQD QFVPNSTSLS DSERVMIITG
860 870 880 890 900
PNMGGKSSYI KQVTLVTIMA QIGSYVPAEE ATIGIVDGIF TRMGAADNIY
910 920 930 940 950
KGRSTFMEQL TDTAEIIRRA SPQSLVILDE LGRGTSTHDG IAIAYATLEY
960 970 980 990 1000
FIRDVKSLTL FVTHYPPVCE LEKCYPEQVG NYHMGFLVNE DESKQDSGDM
1010 1020 1030 1040 1050
EQMPDSVTFL YQITRGIAAR SYGLNVAKLA DVPREVLQKA AHKSKELEGL
1060 1070 1080 1090
VSLRRKRLEC FTDLWMTHSV KDLHTWADKL EMEEIQTSLP H
Length:1,091
Mass (Da):123,074
Last modified:July 1, 1993 - v3
Checksum:i822BF422436FD513
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti505 – 5062MV → IL in AAB60711 (PubMed:7926796).Curated
Sequence conflicti864 – 8641T → A in AAB60711 (PubMed:7926796).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80360 mRNA. Translation: AAA40052.1.
L10319
, L10295, L10296, L10297, L10298, L10299, L10300, L10301, L10304, L10305, L10306, L10307, L10308, L10309, L10310, L10311, L10312, L10313, L10314, L10315, L10316, L10317, L10318 Genomic DNA. Translation: AAB60711.1.
M24919 mRNA. Translation: AAA40051.1. Sequence problems.
PIRiA32495.
RefSeqiNP_034959.2. NM_010829.2.
UniGeneiMm.343101.

Genome annotation databases

GeneIDi17686.
KEGGimmu:17686.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80360 mRNA. Translation: AAA40052.1.
L10319
, L10295, L10296, L10297, L10298, L10299, L10300, L10301, L10304, L10305, L10306, L10307, L10308, L10309, L10310, L10311, L10312, L10313, L10314, L10315, L10316, L10317, L10318 Genomic DNA. Translation: AAB60711.1.
M24919 mRNA. Translation: AAA40051.1. Sequence problems.
PIRiA32495.
RefSeqiNP_034959.2. NM_010829.2.
UniGeneiMm.343101.

3D structure databases

ProteinModelPortaliP13705.
SMRiP13705. Positions 183-1076.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201526. 1 interaction.
STRINGi10090.ENSMUSP00000022220.

PTM databases

iPTMnetiP13705.
PhosphoSiteiP13705.

Proteomic databases

EPDiP13705.
MaxQBiP13705.
PaxDbiP13705.
PRIDEiP13705.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi17686.
KEGGimmu:17686.

Organism-specific databases

CTDi4437.
MGIiMGI:109519. Msh3.

Phylogenomic databases

eggNOGiKOG0217. Eukaryota.
COG0249. LUCA.
HOVERGENiHBG006400.
InParanoidiP13705.
KOiK08736.
PhylomeDBiP13705.

Miscellaneous databases

ChiTaRSiMsh3. mouse.
NextBioi292256.
PROiP13705.
SOURCEiSearch...

Gene expression databases

CleanExiMM_MSH3.

Family and domain databases

Gene3Di3.30.420.110. 1 hit.
3.40.1170.10. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR007695. DNA_mismatch_repair_MutS-lik_N.
IPR000432. DNA_mismatch_repair_MutS_C.
IPR007696. DNA_mismatch_repair_MutS_core.
IPR016151. DNA_mismatch_repair_MutS_N.
IPR007860. DNA_mmatch_repair_MutS_con_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01624. MutS_I. 1 hit.
PF05188. MutS_II. 1 hit.
PF05192. MutS_III. 1 hit.
PF00488. MutS_V. 1 hit.
[Graphical view]
SMARTiSM00534. MUTSac. 1 hit.
SM00533. MUTSd. 1 hit.
[Graphical view]
SUPFAMiSSF48334. SSF48334. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF53150. SSF53150. 2 hits.
SSF55271. SSF55271. 1 hit.
PROSITEiPS00486. DNA_MISMATCH_REPAIR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Liu K., Niu L., Linton J.P., Crouse G.F.
    Submitted (APR-1992) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Characterization of the mouse Rep-3 gene: sequence similarities to bacterial and yeast mismatch-repair proteins."
    Liu K., Niu L., Linton J.P., Crouse G.F.
    Gene 147:169-177(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Dual bidirectional promoters at the mouse dhfr locus: cloning and characterization of two mRNA classes of the divergently transcribed Rep-1 gene."
    Linton J.P., Yen J.-Y.J., Selby E., Chen Z., Chinsky J.M., Liu K., Kellems R.E., Crouse G.F.
    Mol. Cell. Biol. 9:3058-3072(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-917.

Entry informationi

Entry nameiMSH3_MOUSE
AccessioniPrimary (citable) accession number: P13705
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: July 1, 1993
Last modified: May 11, 2016
This is version 122 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.