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P13704 (HMCS1_CRIGR) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydroxymethylglutaryl-CoA synthase, cytoplasmic

Short name=HMG-CoA synthase
EC=2.3.3.10
Alternative name(s):
3-hydroxy-3-methylglutaryl coenzyme A synthase
Gene names
Name:HMGCS1
Synonyms:HMGCS
OrganismCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Taxonomic identifier10029 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeCricetulus

Protein attributes

Sequence length520 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.

Catalytic activity

Acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA.

Pathway

Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 2/3.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the HMG-CoA synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 520520Hydroxymethylglutaryl-CoA synthase, cytoplasmic
PRO_0000213746

Sites

Active site951Proton donor/acceptor By similarity
Active site1291Acyl-thioester intermediate By similarity
Active site2641Proton donor/acceptor By similarity

Amino acid modifications

Modified residue41Phosphoserine By similarity
Modified residue461N6-acetyllysine By similarity
Modified residue2731N6-acetyllysine By similarity
Modified residue4951Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
P13704 [UniParc].

Last modified January 1, 1990. Version 1.
Checksum: 2D4CAAE7DEE5D6BB

FASTA52057,318
        10         20         30         40         50         60 
MPGSLPLNAE ACWPKDVGIV ALEIYFPSQY VDQAELEKYD GVDAGKYTIG LGQARMGFCT 

        70         80         90        100        110        120 
DREDINSLCL TVVQNLMERN SLSYDCIGRL EVGTETIIDK SKSVKSNLMQ LFEESGNTDI 

       130        140        150        160        170        180 
EGIDTTNACY GGTAAVFNAV NWIESSSWDG RYALVVAGDI AIYATGNARP TGGVGAVALL 

       190        200        210        220        230        240 
IGPNAPLIFD RGLRGTHMQH AYDFYKPDML SEYPIVDGKL SIQCYLSALD RCYSVYRKKI 

       250        260        270        280        290        300 
RAQWQKEGND NDFTLNDFGF MISHSPYCKL VQKSLARMFL NDFLNDQNRD KNSIYSGLEA 

       310        320        330        340        350        360 
FGDVKLEDTY FDRDVEKAFM KASSELFNQK TKASLLVSNQ NGNMYTSSVY GSLASVLAQY 

       370        380        390        400        410        420 
SPQQLAGKRI GVFSYGSGLA ATLYSLKVTQ DATPGSALDK VTASLCDLKS RLDSRTCVAP 

       430        440        450        460        470        480 
DVFAENMKLR EDTHHLANYI PQCSIDSLFE GTWYLVRVDE KHRRTYARRP STNDHNLGDG 

       490        500        510        520 
VGLVHSNTAT EHIPSPAKKV PRLPATAAES ESAVISNGEH 

« Hide

References

[1]"Cytoplasmic 3-hydroxy-3-methylglutaryl coenzyme A synthase from the hamster. I. Isolation and sequencing of a full-length cDNA."
Gil G., Goldstein J.L., Slaughter C.A., Brown M.S.
J. Biol. Chem. 261:3710-3716(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Ovary.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L00334 expand/collapse EMBL AC list , L00326, L00327, L00328, L00329, L00330, L00331, L00332, L00333 Genomic DNA. Translation: AAA37076.1.
PIRA25332.

3D structure databases

ProteinModelPortalP13704.
SMRP13704. Positions 16-470.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP13704.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

HOVERGENHBG051912.

Enzyme and pathway databases

BRENDA2.3.3.10. 1309.
UniPathwayUPA00058; UER00102.

Family and domain databases

Gene3D3.40.47.10. 1 hit.
InterProIPR000590. HMG_CoA_synt_AS.
IPR013746. HMG_CoA_synt_C.
IPR013528. HMG_CoA_synth_N.
IPR010122. HMG_CoA_synthase_euk.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamPF08540. HMG_CoA_synt_C. 1 hit.
PF01154. HMG_CoA_synt_N. 1 hit.
[Graphical view]
SUPFAMSSF53901. SSF53901. 3 hits.
TIGRFAMsTIGR01833. HMG-CoA-S_euk. 1 hit.
PROSITEPS01226. HMG_COA_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHMCS1_CRIGR
AccessionPrimary (citable) accession number: P13704
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: June 11, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways