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Protein

NADP-dependent malic enzyme

Gene

Me1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-malate + NADP+ = pyruvate + CO2 + NADPH.
Oxaloacetate = pyruvate + CO2.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Divalent metal cations. Prefers magnesium or manganese.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei102Proton donorBy similarity1
Binding sitei155NADPBy similarity1
Active sitei173Proton acceptorBy similarity1
Metal bindingi245Divalent metal cationBy similarity1
Metal bindingi246Divalent metal cationBy similarity1
Metal bindingi269Divalent metal cationBy similarity1
Binding sitei269NADPBy similarity1
Sitei269Important for activityBy similarity1
Binding sitei408NADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi301 – 318NADPBy similarityAdd BLAST18

GO - Molecular functioni

  • malate dehydrogenase (decarboxylating) (NAD+) activity Source: InterPro
  • malate dehydrogenase (decarboxylating) (NADP+) activity Source: UniProtKB
  • malic enzyme activity Source: UniProtKB
  • manganese ion binding Source: UniProtKB
  • NAD binding Source: InterPro
  • NADP binding Source: RGD
  • oxaloacetate decarboxylase activity Source: UniProtKB-EC

GO - Biological processi

  • malate metabolic process Source: UniProtKB
  • response to hormone Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, NADP

Enzyme and pathway databases

SABIO-RKP13697.

Names & Taxonomyi

Protein namesi
Recommended name:
NADP-dependent malic enzyme (EC:1.1.1.40)
Short name:
NADP-ME
Alternative name(s):
Malic enzyme 1
Gene namesi
Name:Me1
Synonyms:Mod-1, Mod1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3074. Me1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • mitochondrion Source: CACAO
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2293.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001601951 – 572NADP-dependent malic enzymeAdd BLAST572

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei336PhosphoserineCombined sources1

Post-translational modificationi

ISGylated.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP13697.
PRIDEiP13697.

2D gel databases

World-2DPAGE0004:P13697.

PTM databases

iPTMnetiP13697.
PhosphoSitePlusiP13697.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013244.

Structurei

3D structure databases

ProteinModelPortaliP13697.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the malic enzymes family.Curated

Phylogenomic databases

eggNOGiKOG1257. Eukaryota.
COG0281. LUCA.
HOGENOMiHOG000042486.
HOVERGENiHBG000746.
InParanoidiP13697.
PhylomeDBiP13697.

Family and domain databases

Gene3Di3.40.50.10380. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR015884. Malic_enzyme_CS.
IPR012301. Malic_N_dom.
IPR012302. Malic_NAD-bd.
IPR001891. Malic_OxRdtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00390. malic. 1 hit.
PF03949. Malic_M. 1 hit.
[Graphical view]
PIRSFiPIRSF000106. ME. 1 hit.
PRINTSiPR00072. MALOXRDTASE.
SMARTiSM01274. malic. 1 hit.
SM00919. Malic_M. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00331. MALIC_ENZYMES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P13697-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPRAPRRRH THQRGYLLTR DPHLNKDLAF TLEERQQLKI HGLLPPCIVN
60 70 80 90 100
QEIQVLRVIK NFERLNSDFD RYLLLMDLQD RNEKLFYSVL MSNVEKFMPI
110 120 130 140 150
VYTPTVGLAC QQYSLAFRKP RGLFISIHDK GHIASVLNAW PEDVVKAIVV
160 170 180 190 200
TDGERILGLG DLGCNGMGIP VGKLALYTAC GGVNPQQCLP ITLDVGTENE
210 220 230 240 250
ELLKDPLYIG LRHRRVRGPE YDAFLDEFME AASSKYGMNC LIQFEDFANL
260 270 280 290 300
NAFRLLNKYR NKYCTFNDDI QGTASVAVAG LLAALRITKN KLSDQTVLFQ
310 320 330 340 350
GAGEAALGIA HLIVMAMEKE GLSKEKARQK IWLVDSKGLI VKGRASLTEE
360 370 380 390 400
KEVFAHEHEE MKNLEAIVQK IKPTALIGVA AIGGAFTEQI LKDMAAFNER
410 420 430 440 450
PIIFALSNPT SKAECSAEEC YKVTKGRAIF ASGSPFDPVT LPDGRTLFPG
460 470 480 490 500
QGNNSYVFPG VALGVVACGL RHINDSVFLT TAEVISQQVS DKHLEEGRLY
510 520 530 540 550
PPLNTIRDVS LKIAVKIVQD AYKEKMATVY PEPQNKEEFV SSQMYSTNYD
560 570
QILPDCYSWP EEVQKIQTKV NQ
Length:572
Mass (Da):64,003
Last modified:April 1, 1993 - v2
Checksum:i7D6EB48F3BA7D95B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26594
, M26581, M26582, M26583, M26584, M26585, M26586, M26587, M26588, M26589, M26590, M26591, M26592, M26593 Genomic DNA. Translation: AAA41563.1. Sequence problems.
PIRiA37228. DERTMX.
UniGeneiRn.3519.

Genome annotation databases

UCSCiRGD:3074. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26594
, M26581, M26582, M26583, M26584, M26585, M26586, M26587, M26588, M26589, M26590, M26591, M26592, M26593 Genomic DNA. Translation: AAA41563.1. Sequence problems.
PIRiA37228. DERTMX.
UniGeneiRn.3519.

3D structure databases

ProteinModelPortaliP13697.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013244.

Chemistry databases

ChEMBLiCHEMBL2293.

PTM databases

iPTMnetiP13697.
PhosphoSitePlusiP13697.

2D gel databases

World-2DPAGE0004:P13697.

Proteomic databases

PaxDbiP13697.
PRIDEiP13697.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:3074. rat.

Organism-specific databases

RGDi3074. Me1.

Phylogenomic databases

eggNOGiKOG1257. Eukaryota.
COG0281. LUCA.
HOGENOMiHOG000042486.
HOVERGENiHBG000746.
InParanoidiP13697.
PhylomeDBiP13697.

Enzyme and pathway databases

SABIO-RKP13697.

Miscellaneous databases

PROiP13697.

Family and domain databases

Gene3Di3.40.50.10380. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR015884. Malic_enzyme_CS.
IPR012301. Malic_N_dom.
IPR012302. Malic_NAD-bd.
IPR001891. Malic_OxRdtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00390. malic. 1 hit.
PF03949. Malic_M. 1 hit.
[Graphical view]
PIRSFiPIRSF000106. ME. 1 hit.
PRINTSiPR00072. MALOXRDTASE.
SMARTiSM01274. malic. 1 hit.
SM00919. Malic_M. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00331. MALIC_ENZYMES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMAOX_RAT
AccessioniPrimary (citable) accession number: P13697
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: April 1, 1993
Last modified: November 2, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.