Skip Header

Contribute Send feedback
Read comments (?) or add your own

P13697 (MAOX_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified October 19, 2011. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADP-dependent malic enzyme
Short name=NADP-ME
EC=1.1.1.40
Alternative name(s):
Malic enzyme 1
Gene names
Name:Me1
Synonyms:Mod-1, Mod1
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length572 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

(S)-malate + NADP+ = pyruvate + CO2 + NADPH.

Cofactor

Divalent metal cations. Prefers magnesium or manganese By similarity.

Subunit structure

Homotetramer.

Subcellular location

Cytoplasm.

Post-translational modification

ISGylated By similarity.

Sequence similarities

Belongs to the malic enzymes family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 572572NADP-dependent malic enzyme
PRO_0000160195

Regions

Nucleotide binding301 – 31818NADP By similarity

Sites

Active site1021Proton donor By similarity
Active site1731Proton acceptor By similarity
Metal binding2451Divalent metal cation By similarity
Metal binding2461Divalent metal cation By similarity
Metal binding2691Divalent metal cation By similarity
Binding site1551NADP By similarity
Binding site2691NADP By similarity
Binding site4081NADP By similarity
Site2691Important for activity By similarity

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue601N6-acetyllysine By similarity
Modified residue841N6-acetyllysine By similarity

Sequences

Sequence LengthMass (Da)Tools
P13697 [UniParc].

Last modified April 1, 1993. Version 2.
Checksum: 7D6EB48F3BA7D95B

FASTA57264,003
        10         20         30         40         50         60 
MDPRAPRRRH THQRGYLLTR DPHLNKDLAF TLEERQQLKI HGLLPPCIVN QEIQVLRVIK 

        70         80         90        100        110        120 
NFERLNSDFD RYLLLMDLQD RNEKLFYSVL MSNVEKFMPI VYTPTVGLAC QQYSLAFRKP 

       130        140        150        160        170        180 
RGLFISIHDK GHIASVLNAW PEDVVKAIVV TDGERILGLG DLGCNGMGIP VGKLALYTAC 

       190        200        210        220        230        240 
GGVNPQQCLP ITLDVGTENE ELLKDPLYIG LRHRRVRGPE YDAFLDEFME AASSKYGMNC 

       250        260        270        280        290        300 
LIQFEDFANL NAFRLLNKYR NKYCTFNDDI QGTASVAVAG LLAALRITKN KLSDQTVLFQ 

       310        320        330        340        350        360 
GAGEAALGIA HLIVMAMEKE GLSKEKARQK IWLVDSKGLI VKGRASLTEE KEVFAHEHEE 

       370        380        390        400        410        420 
MKNLEAIVQK IKPTALIGVA AIGGAFTEQI LKDMAAFNER PIIFALSNPT SKAECSAEEC 

       430        440        450        460        470        480 
YKVTKGRAIF ASGSPFDPVT LPDGRTLFPG QGNNSYVFPG VALGVVACGL RHINDSVFLT 

       490        500        510        520        530        540 
TAEVISQQVS DKHLEEGRLY PPLNTIRDVS LKIAVKIVQD AYKEKMATVY PEPQNKEEFV 

       550        560        570 
SSQMYSTNYD QILPDCYSWP EEVQKIQTKV NQ

« Hide

References

[1]"Coding nucleotide sequence of rat malic enzyme mRNA and tissue specific regulation by thyroid hormone."
Nikodem V.M., Magnuson M.A., Dozin B., Morioka H.
Endocr. Res. 15:547-564(1989) [PubMed: 2699453] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]"Coding nucleotide sequence of rat liver malic enzyme mRNA."
Magnuson M.A., Morioka H., Tecce M.F., Nikodem V.M.
J. Biol. Chem. 261:1183-1186(1986) [PubMed: 3753699] [Abstract]
Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE.
Strain: Sprague-Dawley.
[3]"Structural and functional analysis of the rat malic enzyme gene promoter."
Morioka H., Tennyson G.E., Nikodem V.M.
Mol. Cell. Biol. 8:3542-3545(1988) [PubMed: 3211151] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-35.
[4]Lubec G., Afjehi-Sadat L., Chen W.-Q.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 15-20; 27-35; 65-81; 147-155; 263-286; 428-445 AND 499-507, MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Hippocampus and Spinal cord.
[5]"Structural characterization of the rat malic enzyme gene."
Morioka H., Magnuson M.A., Mitsuhashi T., Song M.-K.H., Rall J.E., Nikodem V.M.
Proc. Natl. Acad. Sci. U.S.A. 86:4912-4916(1989) [PubMed: 2740332] [Abstract]
Cited for: PARTIAL PROTEIN SEQUENCE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M26594 expand/collapse EMBL AC list , M26581, M26582, M26583, M26584, M26585, M26586, M26587, M26588, M26589, M26590, M26591, M26592, M26593 Genomic DNA. Translation: AAA41563.1. Sequence problems.
IPIIPI00231786.
PIRDERTMX. A37228.
UniGeneRn.3519.

3D structure databases

ProteinModelPortalP13697.
SMRP13697. Positions 15-555.
ModBaseSearch...

Protein-protein interaction databases

STRINGP13697.

2D gel databases

World-2DPAGE0004:P13697.

Proteomic databases

PRIDEP13697.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

RGD3074. Me1.

Phylogenomic databases

eggNOGmaNOG04628.
GeneTreeENSGT00390000000754.
HOVERGENHBG000746.
InParanoidP13697.
OrthoDBEOG44BB1T.
PhylomeDBP13697.

Gene expression databases

ArrayExpressP13697.
GenevestigatorP13697.
GermOnlineENSRNOG00000009715. Rattus norvegicus.

Family and domain databases

InterProIPR015884. Malic_enzyme_CS.
IPR012301. Malic_N.
IPR012302. Malic_NAD-bd.
IPR001891. Malic_OxRdtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.10380. G3DSA:3.40.50.10380. 1 hit.
G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PfamPF00390. malic. 1 hit.
PF03949. Malic_M. 1 hit.
[Graphical view]
PIRSFPIRSF000106. ME. 1 hit.
PRINTSPR00072. MALOXRDTASE.
SMARTSM00919. Malic_M. 1 hit.
[Graphical view]
PROSITEPS00331. MALIC_ENZYMES. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMAOX_RAT
AccessionPrimary (citable) accession number: P13697
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: April 1, 1993
Last modified: October 19, 2011
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents