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Protein

NADP-dependent malic enzyme

Gene

Me1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-malate + NADP+ = pyruvate + CO2 + NADPH.
Oxaloacetate = pyruvate + CO2.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Divalent metal cations. Prefers magnesium or manganese.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei102Proton donorBy similarity1
Binding sitei155NADPBy similarity1
Active sitei173Proton acceptorBy similarity1
Metal bindingi245Divalent metal cationBy similarity1
Metal bindingi246Divalent metal cationBy similarity1
Metal bindingi269Divalent metal cationBy similarity1
Binding sitei269NADPBy similarity1
Sitei269Important for activityBy similarity1
Binding sitei408NADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi301 – 318NADPBy similarityAdd BLAST18

GO - Molecular functioni

  • malate dehydrogenase (decarboxylating) (NAD+) activity Source: InterPro
  • malate dehydrogenase (decarboxylating) (NADP+) activity Source: UniProtKB
  • malic enzyme activity Source: RGD
  • manganese ion binding Source: UniProtKB
  • NAD binding Source: InterPro
  • NADP binding Source: RGD
  • oxaloacetate decarboxylase activity Source: UniProtKB-EC

GO - Biological processi

  • malate metabolic process Source: UniProtKB
  • pyruvate metabolic process Source: GO_Central
  • response to hormone Source: UniProtKB

Keywordsi

Molecular functionOxidoreductase
LigandMetal-binding, NADP

Enzyme and pathway databases

SABIO-RKP13697

Names & Taxonomyi

Protein namesi
Recommended name:
NADP-dependent malic enzyme (EC:1.1.1.40)
Short name:
NADP-ME
Alternative name(s):
Malic enzyme 1
Gene namesi
Name:Me1
Synonyms:Mod-1, Mod1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3074 Me1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2293

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001601951 – 572NADP-dependent malic enzymeAdd BLAST572

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei336PhosphoserineCombined sources1

Post-translational modificationi

ISGylated.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP13697
PRIDEiP13697

2D gel databases

World-2DPAGE0004:P13697

PTM databases

iPTMnetiP13697
PhosphoSitePlusiP13697

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013244

Structurei

3D structure databases

ProteinModelPortaliP13697
SMRiP13697
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the malic enzymes family.Curated

Phylogenomic databases

eggNOGiKOG1257 Eukaryota
COG0281 LUCA
HOGENOMiHOG000042486
HOVERGENiHBG000746
InParanoidiP13697
PhylomeDBiP13697

Family and domain databases

Gene3Di3.40.50.10380, 2 hits
InterProiView protein in InterPro
IPR015884 Malic_enzyme_CS
IPR012301 Malic_N_dom
IPR037062 Malic_N_dom_sf
IPR012302 Malic_NAD-bd
IPR001891 Malic_OxRdtase
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF00390 malic, 1 hit
PF03949 Malic_M, 1 hit
PIRSFiPIRSF000106 ME, 1 hit
PRINTSiPR00072 MALOXRDTASE
SMARTiView protein in SMART
SM01274 malic, 1 hit
SM00919 Malic_M, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00331 MALIC_ENZYMES, 1 hit

Sequencei

Sequence statusi: Complete.

P13697-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPRAPRRRH THQRGYLLTR DPHLNKDLAF TLEERQQLKI HGLLPPCIVN
60 70 80 90 100
QEIQVLRVIK NFERLNSDFD RYLLLMDLQD RNEKLFYSVL MSNVEKFMPI
110 120 130 140 150
VYTPTVGLAC QQYSLAFRKP RGLFISIHDK GHIASVLNAW PEDVVKAIVV
160 170 180 190 200
TDGERILGLG DLGCNGMGIP VGKLALYTAC GGVNPQQCLP ITLDVGTENE
210 220 230 240 250
ELLKDPLYIG LRHRRVRGPE YDAFLDEFME AASSKYGMNC LIQFEDFANL
260 270 280 290 300
NAFRLLNKYR NKYCTFNDDI QGTASVAVAG LLAALRITKN KLSDQTVLFQ
310 320 330 340 350
GAGEAALGIA HLIVMAMEKE GLSKEKARQK IWLVDSKGLI VKGRASLTEE
360 370 380 390 400
KEVFAHEHEE MKNLEAIVQK IKPTALIGVA AIGGAFTEQI LKDMAAFNER
410 420 430 440 450
PIIFALSNPT SKAECSAEEC YKVTKGRAIF ASGSPFDPVT LPDGRTLFPG
460 470 480 490 500
QGNNSYVFPG VALGVVACGL RHINDSVFLT TAEVISQQVS DKHLEEGRLY
510 520 530 540 550
PPLNTIRDVS LKIAVKIVQD AYKEKMATVY PEPQNKEEFV SSQMYSTNYD
560 570
QILPDCYSWP EEVQKIQTKV NQ
Length:572
Mass (Da):64,003
Last modified:April 1, 1993 - v2
Checksum:i7D6EB48F3BA7D95B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26594
, M26581, M26582, M26583, M26584, M26585, M26586, M26587, M26588, M26589, M26590, M26591, M26592, M26593 Genomic DNA Translation: AAA41563.1 Sequence problems.
PIRiA37228 DERTMX
UniGeneiRn.3519

Genome annotation databases

UCSCiRGD:3074 rat

Similar proteinsi

Entry informationi

Entry nameiMAOX_RAT
AccessioniPrimary (citable) accession number: P13697
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: April 1, 1993
Last modified: May 23, 2018
This is version 142 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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