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P13681

- PP11_SCHPO

UniProt

P13681 - PP11_SCHPO

Protein

Serine/threonine-protein phosphatase PP1-1

Gene

dis2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 1 (01 Jan 1990)
      Previous versions | rss
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    Functioni

    Essential role in cell cycle control. PP1 is perhaps required for exit from mitosis.

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi63 – 631Manganese 1By similarity
    Metal bindingi65 – 651Manganese 1By similarity
    Metal bindingi91 – 911Manganese 1By similarity
    Metal bindingi91 – 911Manganese 2By similarity
    Metal bindingi123 – 1231Manganese 2By similarity
    Active sitei124 – 1241Proton donorBy similarity
    Metal bindingi172 – 1721Manganese 2By similarity
    Metal bindingi247 – 2471Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. phosphoprotein phosphatase activity Source: PomBase
    3. protein binding Source: IntAct
    4. protein serine/threonine phosphatase activity Source: PomBase

    GO - Biological processi

    1. microtubule cytoskeleton organization Source: PomBase
    2. mitotic DNA damage checkpoint Source: PomBase
    3. mitotic nuclear division Source: UniProtKB-KW
    4. protein dephosphorylation Source: PomBase
    5. regulation of homologous chromosome segregation Source: PomBase
    6. regulation of mitotic cell cycle Source: PomBase
    7. rRNA processing Source: PomBase
    8. signal transduction Source: PomBase

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Keywords - Ligandi

    Manganese, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase PP1-1 (EC:3.1.3.16)
    Gene namesi
    Name:dis2
    Synonyms:bws1
    ORF Names:SPBC776.02c
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome II

    Organism-specific databases

    PomBaseiSPBC776.02c.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: PomBase
    2. cytosol Source: PomBase
    3. mRNA cleavage and polyadenylation specificity factor complex Source: PomBase
    4. nucleus Source: PomBase
    5. protein phosphatase type 1 complex Source: PomBase

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi245 – 2451R → Q: Cold-sensitive phenotype.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 327327Serine/threonine-protein phosphatase PP1-1PRO_0000058818Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei316 – 3161Phosphothreonine; by CDC21 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP13681.
    PaxDbiP13681.
    PRIDEiP13681.

    Interactioni

    Subunit structurei

    Oligomer.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    tea4O601322EBI-4320127,EBI-1099982

    Protein-protein interaction databases

    BioGridi277707. 65 interactions.
    IntActiP13681. 2 interactions.
    MINTiMINT-4687271.
    STRINGi4896.SPBC776.02c-1.

    Structurei

    3D structure databases

    ProteinModelPortaliP13681.
    SMRiP13681. Positions 1-308.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PPP phosphatase family. PP-1 subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0639.
    HOGENOMiHOG000172697.
    KOiK06269.
    OMAiMGDVMNI.
    OrthoDBiEOG79KPQ9.
    PhylomeDBiP13681.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P13681-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSNPDVDLDS IIDRLLEVRG SRPGRQVQLS EDEIRFLCNK AREIFISQPI    50
    LLELEAPLKI CGDIHGQYYD LLRLFEYGGF PPEANYLFLG DYVDRGKQSL 100
    EVICLLLAYK IKYPENFFIL RGNHECASIN RIYGFYDECK RRYNIKLWKT 150
    FTDCFNCLPI AAIIDEKIFT MHGGLSPDLN SMDQIQRIMR PTDVPDTGLL 200
    CDLLWSDPDK DLTGWGDNDR GVSFTFGPDV VSRFLHKHDM DLVCRAHQVV 250
    EDGYEFFSKR QLVTLFSAPN YCGEFDNAGA MMSVDESLLC SFQILKPAEK 300
    KQRYGYQGSS QNWHMTPPRK NKTGNSK 327
    Length:327
    Mass (Da):37,636
    Last modified:January 1, 1990 - v1
    Checksum:i1106BB9C7D6FDCFB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti17 – 171E → EGE(PubMed:2544298)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M27075 Genomic DNA. Translation: AAA74731.1.
    M27068 Genomic DNA. Translation: AAA89197.1.
    CU329671 Genomic DNA. Translation: CAA22875.1.
    PIRiA32550.
    RefSeqiNP_596317.1. NM_001022239.2.

    Genome annotation databases

    EnsemblFungiiSPBC776.02c.1; SPBC776.02c.1:pep; SPBC776.02c.
    GeneIDi2541193.
    KEGGispo:SPBC776.02c.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M27075 Genomic DNA. Translation: AAA74731.1 .
    M27068 Genomic DNA. Translation: AAA89197.1 .
    CU329671 Genomic DNA. Translation: CAA22875.1 .
    PIRi A32550.
    RefSeqi NP_596317.1. NM_001022239.2.

    3D structure databases

    ProteinModelPortali P13681.
    SMRi P13681. Positions 1-308.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 277707. 65 interactions.
    IntActi P13681. 2 interactions.
    MINTi MINT-4687271.
    STRINGi 4896.SPBC776.02c-1.

    Proteomic databases

    MaxQBi P13681.
    PaxDbi P13681.
    PRIDEi P13681.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPBC776.02c.1 ; SPBC776.02c.1:pep ; SPBC776.02c .
    GeneIDi 2541193.
    KEGGi spo:SPBC776.02c.

    Organism-specific databases

    PomBasei SPBC776.02c.

    Phylogenomic databases

    eggNOGi COG0639.
    HOGENOMi HOG000172697.
    KOi K06269.
    OMAi MGDVMNI.
    OrthoDBi EOG79KPQ9.
    PhylomeDBi P13681.

    Miscellaneous databases

    NextBioi 20802305.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00156. PP2Ac. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Involvement of a type 1 protein phosphatase encoded by bws1+ in fission yeast mitotic control."
      Booher R., Beach D.
      Cell 57:1009-1016(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: SP557.
    2. "The fission yeast dis2+ gene required for chromosome disjoining encodes one of two putative type 1 protein phosphatases."
      Ohkura H., Kinoshita N., Miyatani S., Toda T., Yanagida M.
      Cell 57:997-1007(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    4. "Distinct, essential roles of type 1 and 2A protein phosphatases in the control of the fission yeast cell division cycle."
      Kinoshita N., Ohkura H., Yanagida M.
      Cell 63:405-415(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTANT CS.
      Strain: 972 / HM123.
    5. "Phosphorylation of dis2 protein phosphatase at the C-terminal cdc2 consensus and its potential role in cell cycle regulation."
      Yamano H., Ishii K., Yanagida M.
      EMBO J. 13:5310-5318(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION.

    Entry informationi

    Entry nameiPP11_SCHPO
    AccessioniPrimary (citable) accession number: P13681
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: January 1, 1990
    Last modified: October 1, 2014
    This is version 128 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3