P13678 (KPC3_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein kinase C
Short name=PKC
EC=2.7.11.13

Alternative name(s):
dPKC98F

Gene names

Name:	Pkc98E
Synonyms:	Pkc3
ORF Names:	CG1954

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	634 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Miscellaneous	This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 2 phorbol-ester/DAG-type zinc fingers. Contains 1 protein kinase domain.
Sequence caution	The sequence AAF56846.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Domain	Repeat Zinc-finger
Ligand	ATP-binding Calcium Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Golgi organization Inferred from mutant phenotype PubMed 21847093. Source: FlyBase intracellular signal transduction Inferred from electronic annotation. Source: InterPro lipid particle organization Inferred from mutant phenotype PubMed 21847093. Source: FlyBase negative regulation of cell proliferation Inferred from mutant phenotype PubMed 21847093. Source: FlyBase neuron projection morphogenesis Inferred from mutant phenotype PubMed 18604272. Source: FlyBase response to ethanol Inferred from direct assay PubMed 18675322. Source: FlyBase
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein kinase C activity Inferred from sequence or structural similarity Ref.1. Source: FlyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 634	634	Protein kinase C		PRO_0000055732
Regions
	Domain	303 – 560	258	Protein kinase
	Domain	561 – 632	72	AGC-kinase C-terminal
	Zinc finger	71 – 121	51	Phorbol-ester/DAG-type 1
	Zinc finger	146 – 196	51	Phorbol-ester/DAG-type 2
	Nucleotide binding	309 – 317	9	ATP By similarity
Sites
	Active site	427	1	Proton acceptor By similarity
	Binding site	332	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P13678 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: 3AE3A3D6B7A276BA	FASTA	634	71,157
	10 20 30 40 50 60 MQSETAVQDL WVNLEPQGKI HVIIELKNRT DKAKAEAVVE HTVAVNKEFK ERAGFNRRRG 70 80 90 100 110 120 AMRRRVHQVN GHKFMATFLR QPTFCSHCRE FIWGIGKQGY QCQVCTLVVH KKCHLSVVSK 130 140 150 160 170 180 CPGMRDEQPA KVEMVPAGQR FNVNLPHRFV VHSYKRFTFC DHCGSLLYGL IKQGLQCETC 190 200 210 220 230 240 GMNVHKRCQK NVANTCGINT KQMAEILSSL GISPDKQQPR RSKYLNQQGG EDNYGASLGA 250 260 270 280 290 300 DGDGAPGQSF RSCALSVDSL ATSTTTMTSG YNSSSCMSLA VTGSGGVGAT GETRPGKCSL 310 320 330 340 350 360 LDFNFIKVLG KGSFGKVMLA EKKGTDEIYA IKVLKKDAII QDDDVDCTMT EKRILALAAN 370 380 390 400 410 420 HPFLTALHSC FQTPDRLFFV MEYVNGGDLM FQIQKARRFE ASRAAFYAAE VTLALQFLHT 430 440 450 460 470 480 HGVIYRDLKL DNILLDQEGH CKLADFGMCK EGIMNGMLTT TFCGTPDYIA PEILKEQEYG 490 500 510 520 530 540 ASVDWWALGV LMYEMMAGQP PFEADNEDEL FDSIMHDDVL YPVWLSREAV SILKGFLTKN 550 560 570 580 590 600 PEQRLGCTGD ENEIRKHPFF AKLDWKELEK RNIKPPFRPK MKNPRDANNF DAEFTKEDPV 610 620 630 LTPIGNEVVR CINQDEFAGF SFVNPKFGPE RKVY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of two new Drosophila protein kinase C genes, including one specifically expressed in photoreceptor cells." Schaeffer E., Smith D., Mardon G., Quinn W., Zuker C. Cell 57:403-412(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J04848 mRNA. Translation: AAA28818.1. AE014297 Genomic DNA. Translation: AAF56846.1. Different initiation.
PIR	B32392.
RefSeq	NP_524545.2. NM_079821.3.
UniGene	Dm.4053.
3D structure databases
ProteinModelPortal	P13678.
SMR	P13678. Positions 70-196, 302-625.
ModBase	Search...
Protein-protein interaction databases
IntAct	P13678. 1 interaction.
MINT	MINT-155042.
Proteomic databases
PaxDb	P13678.
PRIDE	P13678.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	43428.
KEGG	dme:Dmel_CG1954.
Organism-specific databases
CTD	43428.
FlyBase	FBgn0003093. Pkc98E.
Phylogenomic databases
eggNOG	COG0515.
InParanoid	P13678.
KO	K06068.
OrthoDB	EOG42NGFB.
Enzyme and pathway databases
BRENDA	2.7.11.13. 1994.
Gene expression databases
Bgee	P13678.
GermOnline	CG1954. Drosophila melanogaster.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR020454. DAG/PE-bd. IPR011009. Kinase-like_dom. IPR017892. Pkinase_C. IPR014376. Prot_kin_PKC_delta. IPR002219. Prot_Kinase_C-like_PE/DAG-bd. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00130. C1_1. 2 hits. PF00069. Pkinase. 1 hit. PF00433. Pkinase_C. 1 hit. [Graphical view]
PIRSF	PIRSF000551. PKC_delta. 1 hit.
PRINTS	PR00008. DAGPEDOMAIN.
SMART	SM00109. C1. 2 hits. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS00479. ZF_DAG_PE_1. 2 hits. PS50081. ZF_DAG_PE_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	Pkc98E. drosophila.
GenomeRNAi	43428.
NextBio	833872.

Entry information

Entry name

KPC3_DROME

Accession

Primary (citable) accession number: P13678
Secondary accession number(s): Q9VAQ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	May 1, 2013
This is version 134 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project