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Protein

Protein kinase C

Gene

Pkc98E

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei332ATPPROSITE-ProRule annotation1
Active sitei427Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri71 – 121Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri146 – 196Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST51
Nucleotide bindingi309 – 317ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • protein kinase C activity Source: FlyBase
  • protein serine/threonine kinase activity Source: GO_Central

GO - Biological processi

  • dorsal/ventral axis specification Source: FlyBase
  • Golgi organization Source: FlyBase
  • I-kappaB phosphorylation Source: FlyBase
  • lipid particle organization Source: FlyBase
  • long-term memory Source: FlyBase
  • negative regulation of cell proliferation Source: FlyBase
  • neuron projection morphogenesis Source: FlyBase
  • peptidyl-serine phosphorylation Source: GO_Central
  • positive regulation of multicellular organism growth Source: FlyBase
  • positive regulation of peptide hormone secretion Source: FlyBase
  • protein phosphorylation Source: FlyBase
  • response to ethanol Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.11.13. 1994.
ReactomeiR-DME-114508. Effects of PIP2 hydrolysis.
R-DME-1489509. DAG and IP3 signaling.
R-DME-2029485. Role of phospholipids in phagocytosis.
SignaLinkiP13678.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein kinase C (EC:2.7.11.13)
Short name:
PKC
Alternative name(s):
dPKC98F
Gene namesi
Name:Pkc98E
Synonyms:Pkc3
ORF Names:CG1954
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0003093. Pkc98E.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000557321 – 634Protein kinase CAdd BLAST634

Proteomic databases

PaxDbiP13678.
PRIDEiP13678.

Expressioni

Gene expression databases

BgeeiFBgn0003093.
GenevisibleiP13678. DM.

Interactioni

Protein-protein interaction databases

BioGridi68299. 16 interactors.
IntActiP13678. 1 interactor.
MINTiMINT-155042.
STRINGi7227.FBpp0084728.

Structurei

3D structure databases

ProteinModelPortaliP13678.
SMRiP13678.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini303 – 560Protein kinasePROSITE-ProRule annotationAdd BLAST258
Domaini561 – 632AGC-kinase C-terminalAdd BLAST72

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 2 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri71 – 121Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri146 – 196Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST51

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0694. Eukaryota.
ENOG410XNPH. LUCA.
InParanoidiP13678.
KOiK18050.
OrthoDBiEOG091G0QRS.
PhylomeDBiP13678.

Family and domain databases

CDDicd00029. C1. 2 hits.
InterProiIPR000961. AGC-kinase_C.
IPR020454. DAG/PE-bd.
IPR011009. Kinase-like_dom.
IPR002219. PE/DAG-bd.
IPR017892. Pkinase_C.
IPR014376. Prot_kin_PKC_delta.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00130. C1_1. 2 hits.
PF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000551. PKC_delta. 1 hit.
PRINTSiPR00008. DAGPEDOMAIN.
SMARTiSM00109. C1. 2 hits.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P13678-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQSETAVQDL WVNLEPQGKI HVIIELKNRT DKAKAEAVVE HTVAVNKEFK
60 70 80 90 100
ERAGFNRRRG AMRRRVHQVN GHKFMATFLR QPTFCSHCRE FIWGIGKQGY
110 120 130 140 150
QCQVCTLVVH KKCHLSVVSK CPGMRDEQPA KVEMVPAGQR FNVNLPHRFV
160 170 180 190 200
VHSYKRFTFC DHCGSLLYGL IKQGLQCETC GMNVHKRCQK NVANTCGINT
210 220 230 240 250
KQMAEILSSL GISPDKQQPR RSKYLNQQGG EDNYGASLGA DGDGAPGQSF
260 270 280 290 300
RSCALSVDSL ATSTTTMTSG YNSSSCMSLA VTGSGGVGAT GETRPGKCSL
310 320 330 340 350
LDFNFIKVLG KGSFGKVMLA EKKGTDEIYA IKVLKKDAII QDDDVDCTMT
360 370 380 390 400
EKRILALAAN HPFLTALHSC FQTPDRLFFV MEYVNGGDLM FQIQKARRFE
410 420 430 440 450
ASRAAFYAAE VTLALQFLHT HGVIYRDLKL DNILLDQEGH CKLADFGMCK
460 470 480 490 500
EGIMNGMLTT TFCGTPDYIA PEILKEQEYG ASVDWWALGV LMYEMMAGQP
510 520 530 540 550
PFEADNEDEL FDSIMHDDVL YPVWLSREAV SILKGFLTKN PEQRLGCTGD
560 570 580 590 600
ENEIRKHPFF AKLDWKELEK RNIKPPFRPK MKNPRDANNF DAEFTKEDPV
610 620 630
LTPIGNEVVR CINQDEFAGF SFVNPKFGPE RKVY
Length:634
Mass (Da):71,157
Last modified:April 1, 1990 - v1
Checksum:i3AE3A3D6B7A276BA
GO

Sequence cautioni

The sequence AAF56846 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04848 mRNA. Translation: AAA28818.1.
AE014297 Genomic DNA. Translation: AAF56846.1. Different initiation.
PIRiB32392.
RefSeqiNP_001287577.1. NM_001300648.1.
NP_524545.2. NM_079821.4.
UniGeneiDm.4053.

Genome annotation databases

GeneIDi43428.
KEGGidme:Dmel_CG1954.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04848 mRNA. Translation: AAA28818.1.
AE014297 Genomic DNA. Translation: AAF56846.1. Different initiation.
PIRiB32392.
RefSeqiNP_001287577.1. NM_001300648.1.
NP_524545.2. NM_079821.4.
UniGeneiDm.4053.

3D structure databases

ProteinModelPortaliP13678.
SMRiP13678.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi68299. 16 interactors.
IntActiP13678. 1 interactor.
MINTiMINT-155042.
STRINGi7227.FBpp0084728.

Proteomic databases

PaxDbiP13678.
PRIDEiP13678.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi43428.
KEGGidme:Dmel_CG1954.

Organism-specific databases

CTDi43428.
FlyBaseiFBgn0003093. Pkc98E.

Phylogenomic databases

eggNOGiKOG0694. Eukaryota.
ENOG410XNPH. LUCA.
InParanoidiP13678.
KOiK18050.
OrthoDBiEOG091G0QRS.
PhylomeDBiP13678.

Enzyme and pathway databases

BRENDAi2.7.11.13. 1994.
ReactomeiR-DME-114508. Effects of PIP2 hydrolysis.
R-DME-1489509. DAG and IP3 signaling.
R-DME-2029485. Role of phospholipids in phagocytosis.
SignaLinkiP13678.

Miscellaneous databases

ChiTaRSiPkc98E. fly.
GenomeRNAii43428.
PROiP13678.

Gene expression databases

BgeeiFBgn0003093.
GenevisibleiP13678. DM.

Family and domain databases

CDDicd00029. C1. 2 hits.
InterProiIPR000961. AGC-kinase_C.
IPR020454. DAG/PE-bd.
IPR011009. Kinase-like_dom.
IPR002219. PE/DAG-bd.
IPR017892. Pkinase_C.
IPR014376. Prot_kin_PKC_delta.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00130. C1_1. 2 hits.
PF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000551. PKC_delta. 1 hit.
PRINTSiPR00008. DAGPEDOMAIN.
SMARTiSM00109. C1. 2 hits.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKPC3_DROME
AccessioniPrimary (citable) accession number: P13678
Secondary accession number(s): Q9VAQ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: November 30, 2016
This is version 165 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Miscellaneous

This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.