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Protein

Acylamino-acid-releasing enzyme

Gene

Apeh

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This enzyme catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N-acetylated amino acid and a peptide with a free N-terminus. It preferentially cleaves off Ac-Ala, Ac-Met and Ac-Ser.1 Publication

Catalytic activityi

Cleavage of an N-acetyl or N-formyl amino acid from the N-terminus of a polypeptide.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei587Charge relay systemPROSITE-ProRule annotation1
Active sitei675Charge relay systemPROSITE-ProRule annotation1
Active sitei707Charge relay systemPROSITE-ProRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase

Protein family/group databases

ESTHERiratno-acph ACPH_Peptidase_S9
MEROPSiS09.004

Names & Taxonomyi

Protein namesi
Recommended name:
Acylamino-acid-releasing enzyme (EC:3.4.19.1)
Short name:
AARE
Alternative name(s):
Acyl-peptide hydrolase
Short name:
APH
Acylaminoacyl-peptidase
Gene namesi
Name:Apeh
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2125 Apeh

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001224341 – 732Acylamino-acid-releasing enzymeAdd BLAST732

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1Blocked amino end (Met); alternate1 Publication1
Modified residuei1N-acetylmethionine; alternateBy similarity1
Modified residuei185PhosphoserineBy similarity1
Modified residuei187PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP13676
PRIDEiP13676

PTM databases

iPTMnetiP13676
PhosphoSitePlusiP13676

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000043843

Structurei

3D structure databases

ProteinModelPortaliP13676
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S9C family.Curated

Phylogenomic databases

eggNOGiKOG2100 Eukaryota
COG1506 LUCA
HOGENOMiHOG000007443
HOVERGENiHBG000869
InParanoidiP13676
KOiK01303
PhylomeDBiP13676

Family and domain databases

Gene3Di2.120.10.30, 1 hit
3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR011042 6-blade_b-propeller_TolB-like
IPR029058 AB_hydrolase
IPR002471 Pept_S9_AS
IPR001375 Peptidase_S9
PfamiView protein in Pfam
PF00326 Peptidase_S9, 1 hit
SUPFAMiSSF53474 SSF53474, 1 hit
PROSITEiView protein in PROSITE
PS00708 PRO_ENDOPEP_SER, 1 hit

Sequencei

Sequence statusi: Complete.

P13676-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERQVLLSEP QEAAALYRGL SRQPSLSAAC LGPEVTTQYG GLYRTVHTEW
60 70 80 90 100
TQRDLERMEN IRFCRQYLVF HDGDSVVFAG PAGNSVETRG ELLSRESPSG
110 120 130 140 150
TMKAVLRKAG GTVSGEEKQF LEVWEKNRKL KSFNLSALEK HGPVYEDDCF
160 170 180 190 200
GCLSWSHSET HLLYVAEKKR PKAESFFQTK ALDISASDDE MARPKKPDQA
210 220 230 240 250
IKGDQFVFYE DWGETMVSKS IPVLCVLDID SGNISVLEGV PENVSPGQAF
260 270 280 290 300
WAPGDTGVVF VGWWHEPFRL GIRYCTNRRS ALYYVDLSGG KCELLSDGSL
310 320 330 340 350
AICSPRLSPD QCRIVYLQYP CLAPHHQCSQ LCLYDWYTKV TSVVVDIVPR
360 370 380 390 400
QLGESFSGIY CSLLPLGCWS ADSQRVVFDS AQRSRQDLFA VDTQTGSITS
410 420 430 440 450
LTAAGSAGSW KLLTIDKDLM VAQFSTPSLP PSLKVGFLPP PGKEQSVSWV
460 470 480 490 500
SLEEAEPIPG IHWGVRVLHP PPDQENVQYA DLDFEAILLQ PSNPPDKTQV
510 520 530 540 550
PMVVMPHGGP HSSFVTAWML FPAMLCKMGF AVLLVNYRGS TGFGQDSILS
560 570 580 590 600
LPGNVGHQDV KDVQFAVEQV LQEEHFDARR VALMGGSHGG FLSCHLIGQY
610 620 630 640 650
PETYSACIAR NPVINIASMM GSTDIPDWCM VETGFPYSNS CLPDLNVWEE
660 670 680 690 700
MLDKSPIKYI PQVKTPVLLM LGQEDRRVPF KQGMEYYRAL KARNVPVRLL
710 720 730
LYPKSNHALS EVEAESDSFM NAVLWLHTHL GS
Length:732
Mass (Da):81,384
Last modified:January 1, 1990 - v1
Checksum:i43F234879E10235B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti625 – 628IPDW → M in CAA33040 (PubMed:2578023).Curated4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04733 mRNA Translation: AAA88506.1
X14915 Genomic DNA Translation: CAA33040.1 Sequence problems.
PIRiA33706 S07624
RefSeqiNP_036632.1, NM_012500.1
UniGeneiRn.11057

Genome annotation databases

GeneIDi24206
KEGGirno:24206
UCSCiRGD:2125 rat

Similar proteinsi

Entry informationi

Entry nameiACPH_RAT
AccessioniPrimary (citable) accession number: P13676
Secondary accession number(s): P14320, P70479
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: May 23, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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