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P13670 (CHB_VIBHA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
N,N'-diacetylchitobiase

Short name=Chitobiase
EC=3.2.1.52
Alternative name(s):
Beta-N-acetylhexosaminidase
N-acetyl-beta-glucosaminidase
Gene names
Name:chb
OrganismVibrio harveyi (Beneckea harveyi)
Taxonomic identifier669 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Protein attributes

Sequence length883 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolysis of terminal, non-reducing N-acetyl-beta-D-glucosamine residues in chitobiose and higher analogs, and in glycoproteins.

Catalytic activity

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.

Pathway

Glycan degradation; chitin degradation.

Subcellular location

Cell outer membrane; Lipid-anchor.

Induction

By chitobiose.

Post-translational modification

This protein is probably a lipoprotein, its processing is inhibited by globomycin.

Sequence similarities

Belongs to the glycosyl hydrolase 20 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717
Chain18 – 883866N,N'-diacetylchitobiase
PRO_0000012019

Sites

Active site5371Proton donor By similarity

Amino acid modifications

Lipidation181N-palmitoyl cysteine Probable
Lipidation181S-diacylglycerol cysteine
Disulfide bond54 ↔ 64 By similarity
Disulfide bond394 ↔ 402 By similarity
Disulfide bond502 ↔ 577 By similarity

Sequences

Sequence LengthMass (Da)Tools
P13670 [UniParc].

Last modified January 1, 1990. Version 1.
Checksum: 8ED14598B1FEEBCE

FASTA88397,771
        10         20         30         40         50         60 
MLKHSLIAAS VITTLAGCSS LQSSEQQVVN SLADNLDIQY EVLTNHGANE GLACQDMGAE 

        70         80         90        100        110        120 
WASCNKVNMT LVNQGEAVDS KDWAIYFHSI RLILDVDNEQ FKISRVTGDL HKLEPTDKFD 

       130        140        150        160        170        180 
GFAAGEEVVL PLVGEYWQLF ETDFMPGAFV SAPNAEPKMI ASLNTEDVAS FVTGLEGNNL 

       190        200        210        220        230        240 
KRTPDDNNVF ANAVSRFEKN EDLATQDVST TLLPTPMHVE AGKGKVDIAD GIALPKDAFD 

       250        260        270        280        290        300 
ATQFAAIQDR AEVVGVDVRG DLPVSITVVP ADFTGELAKS GAYEMSIKGD GIVIKAFDQA 

       310        320        330        340        350        360 
GAFYAVQSIF GLVDSQNADS LPQLSIKDAP RFDYRGVMVD VARNFHSKDA ILATLDQMAA 

       370        380        390        400        410        420 
YKMNKLHLHL TDDEGWRLEI PGLPELTEVG ANRCFDTQEK SCLLPQLGSG PTTDNFGSGY 

       430        440        450        460        470        480 
FSKADYVEIL KYAKARNIEV IPEIDMPAHA RAAVVSMEAR YDRLMEEGKE AEANEYRLMD 

       490        500        510        520        530        540 
PQDTSNVTTV QFYNKQSFIN PCMESSTRFV DKVISEVAAM HQEAGAPLTT WHFGGDEAKN 

       550        560        570        580        590        600 
IKLGAGFQDV NAEDKVSWKG TIDLSKQDKP FAQSPQCQTL ITDGTVSDFA HLPSHFAEEV 

       610        620        630        640        650        660 
SKIVAEKGIP NFQAWQDGLK YSDGEKAFAT ENTRVNFWDV LYWGGTSSVY EWSKKGYDVI 

       670        680        690        700        710        720 
VSNPDYVYMD MPYEVDPKER GYYWATRATD TRKMFGFAPE NMPQNAETSV DRDGNGFTGK 

       730        740        750        760        770        780 
GEIEAKPFYG LSAQLWSETV RNDEQYEYMV FPRVLAAAQR AWHRADWEND YKVGVEYSQN 

       790        800        810        820        830        840 
SNLVDKASLN QDYNRFANVL GQRELAKLEK SGIDYRLPVP GAKVEDGKLA MNVQFPGVTL 

       850        860        870        880 
QYSLDGENWL TYADNARPNV TGEVFIRSVS ATGEKVSRIT SVK 

« Hide

References

[1]"N,N'-diacetylchitobiase of Vibrio harveyi. Primary structure, processing, and evolutionary relationships."
Soto-Gil R.W., Zysking J.W.
J. Biol. Chem. 264:14778-14783(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DIACYLGLYCEROL AT CYS-18.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J05004 Genomic DNA. Translation: AAA88682.1.
PIRA36511.

3D structure databases

ProteinModelPortalP13670.
SMRP13670. Positions 25-882.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH20. Glycoside Hydrolase Family 20.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00349.

Family and domain databases

Gene3D2.60.40.290. 1 hit.
2.60.40.320. 1 hit.
3.20.20.80. 1 hit.
3.30.379.10. 1 hit.
InterProIPR025705. Beta_hexosaminidase_sua/sub.
IPR008965. Carb-bd_dom.
IPR012291. CBD_carb-bd_dom.
IPR004866. CHB/HEX_N_dom.
IPR004867. CHB_HEX_C_dom.
IPR029018. Chitobiase/Hex_dom_2-like.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR015883. Glyco_hydro_20_cat-core.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR015882. HEX_bac_N.
IPR014756. Ig_E-set.
[Graphical view]
PfamPF03173. CHB_HEX. 1 hit.
PF03174. CHB_HEX_C. 1 hit.
PF00728. Glyco_hydro_20. 1 hit.
PF02838. Glyco_hydro_20b. 1 hit.
[Graphical view]
PRINTSPR00738. GLHYDRLASE20.
SMARTSM01081. CHB_HEX. 1 hit.
[Graphical view]
SUPFAMSSF49384. SSF49384. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF55545. SSF55545. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCHB_VIBHA
AccessionPrimary (citable) accession number: P13670
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: June 11, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries