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P13670

- CHB_VIBHA

UniProt

P13670 - CHB_VIBHA

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Protein
N,N'-diacetylchitobiase
Gene
chb
Organism
Vibrio harveyi (Beneckea harveyi)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Hydrolysis of terminal, non-reducing N-acetyl-beta-D-glucosamine residues in chitobiose and higher analogs, and in glycoproteins.

Catalytic activityi

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei537 – 5371Proton donor By similarity

GO - Molecular functioni

  1. beta-N-acetylhexosaminidase activity Source: UniProtKB-EC
  2. polysaccharide binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. chitin catabolic process Source: UniProtKB-UniPathway
  2. polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Chitin degradation, Polysaccharide degradation

Enzyme and pathway databases

UniPathwayiUPA00349.

Protein family/group databases

CAZyiGH20. Glycoside Hydrolase Family 20.

Names & Taxonomyi

Protein namesi
Recommended name:
N,N'-diacetylchitobiase (EC:3.2.1.52)
Short name:
Chitobiase
Alternative name(s):
Beta-N-acetylhexosaminidase
N-acetyl-beta-glucosaminidase
Gene namesi
Name:chb
OrganismiVibrio harveyi (Beneckea harveyi)
Taxonomic identifieri669 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Subcellular locationi

GO - Cellular componenti

  1. cell outer membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717
Add
BLAST
Chaini18 – 883866N,N'-diacetylchitobiase
PRO_0000012019Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi18 – 181N-palmitoyl cysteine Inferred
Lipidationi18 – 181S-diacylglycerol cysteine
Disulfide bondi54 ↔ 64 By similarity
Disulfide bondi394 ↔ 402 By similarity
Disulfide bondi502 ↔ 577 By similarity

Post-translational modificationi

This protein is probably a lipoprotein, its processing is inhibited by globomycin.

Keywords - PTMi

Disulfide bond, Lipoprotein, Palmitate

Expressioni

Inductioni

By chitobiose.

Structurei

3D structure databases

ProteinModelPortaliP13670.
SMRiP13670. Positions 25-882.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.40.290. 1 hit.
2.60.40.320. 1 hit.
3.20.20.80. 1 hit.
3.30.379.10. 1 hit.
InterProiIPR025705. Beta_hexosaminidase_sua/sub.
IPR008965. Carb-bd_dom.
IPR012291. CBD_carb-bd_dom.
IPR004866. CHB/HEX_N_dom.
IPR004867. CHB_HEX_C_dom.
IPR029018. Chitobiase/Hex_dom_2-like.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR015883. Glyco_hydro_20_cat-core.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR015882. HEX_bac_N.
IPR014756. Ig_E-set.
[Graphical view]
PfamiPF03173. CHB_HEX. 1 hit.
PF03174. CHB_HEX_C. 1 hit.
PF00728. Glyco_hydro_20. 1 hit.
PF02838. Glyco_hydro_20b. 1 hit.
[Graphical view]
PRINTSiPR00738. GLHYDRLASE20.
SMARTiSM01081. CHB_HEX. 1 hit.
[Graphical view]
SUPFAMiSSF49384. SSF49384. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF55545. SSF55545. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13670-1 [UniParc]FASTAAdd to Basket

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MLKHSLIAAS VITTLAGCSS LQSSEQQVVN SLADNLDIQY EVLTNHGANE    50
GLACQDMGAE WASCNKVNMT LVNQGEAVDS KDWAIYFHSI RLILDVDNEQ 100
FKISRVTGDL HKLEPTDKFD GFAAGEEVVL PLVGEYWQLF ETDFMPGAFV 150
SAPNAEPKMI ASLNTEDVAS FVTGLEGNNL KRTPDDNNVF ANAVSRFEKN 200
EDLATQDVST TLLPTPMHVE AGKGKVDIAD GIALPKDAFD ATQFAAIQDR 250
AEVVGVDVRG DLPVSITVVP ADFTGELAKS GAYEMSIKGD GIVIKAFDQA 300
GAFYAVQSIF GLVDSQNADS LPQLSIKDAP RFDYRGVMVD VARNFHSKDA 350
ILATLDQMAA YKMNKLHLHL TDDEGWRLEI PGLPELTEVG ANRCFDTQEK 400
SCLLPQLGSG PTTDNFGSGY FSKADYVEIL KYAKARNIEV IPEIDMPAHA 450
RAAVVSMEAR YDRLMEEGKE AEANEYRLMD PQDTSNVTTV QFYNKQSFIN 500
PCMESSTRFV DKVISEVAAM HQEAGAPLTT WHFGGDEAKN IKLGAGFQDV 550
NAEDKVSWKG TIDLSKQDKP FAQSPQCQTL ITDGTVSDFA HLPSHFAEEV 600
SKIVAEKGIP NFQAWQDGLK YSDGEKAFAT ENTRVNFWDV LYWGGTSSVY 650
EWSKKGYDVI VSNPDYVYMD MPYEVDPKER GYYWATRATD TRKMFGFAPE 700
NMPQNAETSV DRDGNGFTGK GEIEAKPFYG LSAQLWSETV RNDEQYEYMV 750
FPRVLAAAQR AWHRADWEND YKVGVEYSQN SNLVDKASLN QDYNRFANVL 800
GQRELAKLEK SGIDYRLPVP GAKVEDGKLA MNVQFPGVTL QYSLDGENWL 850
TYADNARPNV TGEVFIRSVS ATGEKVSRIT SVK 883
Length:883
Mass (Da):97,771
Last modified:January 1, 1990 - v1
Checksum:i8ED14598B1FEEBCE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J05004 Genomic DNA. Translation: AAA88682.1.
PIRiA36511.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J05004 Genomic DNA. Translation: AAA88682.1 .
PIRi A36511.

3D structure databases

ProteinModelPortali P13670.
SMRi P13670. Positions 25-882.
ModBasei Search...

Protein family/group databases

CAZyi GH20. Glycoside Hydrolase Family 20.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00349 .

Family and domain databases

Gene3Di 2.60.40.290. 1 hit.
2.60.40.320. 1 hit.
3.20.20.80. 1 hit.
3.30.379.10. 1 hit.
InterProi IPR025705. Beta_hexosaminidase_sua/sub.
IPR008965. Carb-bd_dom.
IPR012291. CBD_carb-bd_dom.
IPR004866. CHB/HEX_N_dom.
IPR004867. CHB_HEX_C_dom.
IPR029018. Chitobiase/Hex_dom_2-like.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR015883. Glyco_hydro_20_cat-core.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR015882. HEX_bac_N.
IPR014756. Ig_E-set.
[Graphical view ]
Pfami PF03173. CHB_HEX. 1 hit.
PF03174. CHB_HEX_C. 1 hit.
PF00728. Glyco_hydro_20. 1 hit.
PF02838. Glyco_hydro_20b. 1 hit.
[Graphical view ]
PRINTSi PR00738. GLHYDRLASE20.
SMARTi SM01081. CHB_HEX. 1 hit.
[Graphical view ]
SUPFAMi SSF49384. SSF49384. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF55545. SSF55545. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEi PS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "N,N'-diacetylchitobiase of Vibrio harveyi. Primary structure, processing, and evolutionary relationships."
    Soto-Gil R.W., Zysking J.W.
    J. Biol. Chem. 264:14778-14783(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DIACYLGLYCEROL AT CYS-18.

Entry informationi

Entry nameiCHB_VIBHA
AccessioniPrimary (citable) accession number: P13670
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: June 11, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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