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P13670

- CHB_VIBHA

UniProt

P13670 - CHB_VIBHA

Protein

N,N'-diacetylchitobiase

Gene

chb

Organism
Vibrio harveyi (Beneckea harveyi)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 Jan 1990)
      Previous versions | rss
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    Functioni

    Hydrolysis of terminal, non-reducing N-acetyl-beta-D-glucosamine residues in chitobiose and higher analogs, and in glycoproteins.

    Catalytic activityi

    Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei537 – 5371Proton donorBy similarity

    GO - Molecular functioni

    1. beta-N-acetylhexosaminidase activity Source: UniProtKB-EC
    2. polysaccharide binding Source: InterPro

    GO - Biological processi

    1. chitin catabolic process Source: UniProtKB-UniPathway
    2. polysaccharide catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Chitin degradation, Polysaccharide degradation

    Enzyme and pathway databases

    UniPathwayiUPA00349.

    Protein family/group databases

    CAZyiGH20. Glycoside Hydrolase Family 20.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N,N'-diacetylchitobiase (EC:3.2.1.52)
    Short name:
    Chitobiase
    Alternative name(s):
    Beta-N-acetylhexosaminidase
    N-acetyl-beta-glucosaminidase
    Gene namesi
    Name:chb
    OrganismiVibrio harveyi (Beneckea harveyi)
    Taxonomic identifieri669 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

    Subcellular locationi

    GO - Cellular componenti

    1. cell outer membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell outer membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1717Add
    BLAST
    Chaini18 – 883866N,N'-diacetylchitobiasePRO_0000012019Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi18 – 181N-palmitoyl cysteineCurated
    Lipidationi18 – 181S-diacylglycerol cysteine1 PublicationPROSITE-ProRule annotation
    Disulfide bondi54 ↔ 64By similarity
    Disulfide bondi394 ↔ 402By similarity
    Disulfide bondi502 ↔ 577By similarity

    Post-translational modificationi

    This protein is probably a lipoprotein, its processing is inhibited by globomycin.

    Keywords - PTMi

    Disulfide bond, Lipoprotein, Palmitate

    Expressioni

    Inductioni

    By chitobiose.

    Structurei

    3D structure databases

    ProteinModelPortaliP13670.
    SMRiP13670. Positions 25-882.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 20 family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.60.40.290. 1 hit.
    2.60.40.320. 1 hit.
    3.20.20.80. 1 hit.
    3.30.379.10. 1 hit.
    InterProiIPR025705. Beta_hexosaminidase_sua/sub.
    IPR008965. Carb-bd_dom.
    IPR012291. CBD_carb-bd_dom.
    IPR004866. CHB/HEX_N_dom.
    IPR004867. CHB_HEX_C_dom.
    IPR029018. Chitobiase/Hex_dom_2-like.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR015883. Glyco_hydro_20_cat-core.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR015882. HEX_bac_N.
    IPR014756. Ig_E-set.
    [Graphical view]
    PfamiPF03173. CHB_HEX. 1 hit.
    PF03174. CHB_HEX_C. 1 hit.
    PF00728. Glyco_hydro_20. 1 hit.
    PF02838. Glyco_hydro_20b. 1 hit.
    [Graphical view]
    PRINTSiPR00738. GLHYDRLASE20.
    SMARTiSM01081. CHB_HEX. 1 hit.
    [Graphical view]
    SUPFAMiSSF49384. SSF49384. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF55545. SSF55545. 1 hit.
    SSF81296. SSF81296. 1 hit.
    PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P13670-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLKHSLIAAS VITTLAGCSS LQSSEQQVVN SLADNLDIQY EVLTNHGANE    50
    GLACQDMGAE WASCNKVNMT LVNQGEAVDS KDWAIYFHSI RLILDVDNEQ 100
    FKISRVTGDL HKLEPTDKFD GFAAGEEVVL PLVGEYWQLF ETDFMPGAFV 150
    SAPNAEPKMI ASLNTEDVAS FVTGLEGNNL KRTPDDNNVF ANAVSRFEKN 200
    EDLATQDVST TLLPTPMHVE AGKGKVDIAD GIALPKDAFD ATQFAAIQDR 250
    AEVVGVDVRG DLPVSITVVP ADFTGELAKS GAYEMSIKGD GIVIKAFDQA 300
    GAFYAVQSIF GLVDSQNADS LPQLSIKDAP RFDYRGVMVD VARNFHSKDA 350
    ILATLDQMAA YKMNKLHLHL TDDEGWRLEI PGLPELTEVG ANRCFDTQEK 400
    SCLLPQLGSG PTTDNFGSGY FSKADYVEIL KYAKARNIEV IPEIDMPAHA 450
    RAAVVSMEAR YDRLMEEGKE AEANEYRLMD PQDTSNVTTV QFYNKQSFIN 500
    PCMESSTRFV DKVISEVAAM HQEAGAPLTT WHFGGDEAKN IKLGAGFQDV 550
    NAEDKVSWKG TIDLSKQDKP FAQSPQCQTL ITDGTVSDFA HLPSHFAEEV 600
    SKIVAEKGIP NFQAWQDGLK YSDGEKAFAT ENTRVNFWDV LYWGGTSSVY 650
    EWSKKGYDVI VSNPDYVYMD MPYEVDPKER GYYWATRATD TRKMFGFAPE 700
    NMPQNAETSV DRDGNGFTGK GEIEAKPFYG LSAQLWSETV RNDEQYEYMV 750
    FPRVLAAAQR AWHRADWEND YKVGVEYSQN SNLVDKASLN QDYNRFANVL 800
    GQRELAKLEK SGIDYRLPVP GAKVEDGKLA MNVQFPGVTL QYSLDGENWL 850
    TYADNARPNV TGEVFIRSVS ATGEKVSRIT SVK 883
    Length:883
    Mass (Da):97,771
    Last modified:January 1, 1990 - v1
    Checksum:i8ED14598B1FEEBCE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05004 Genomic DNA. Translation: AAA88682.1.
    PIRiA36511.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05004 Genomic DNA. Translation: AAA88682.1 .
    PIRi A36511.

    3D structure databases

    ProteinModelPortali P13670.
    SMRi P13670. Positions 25-882.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH20. Glycoside Hydrolase Family 20.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00349 .

    Family and domain databases

    Gene3Di 2.60.40.290. 1 hit.
    2.60.40.320. 1 hit.
    3.20.20.80. 1 hit.
    3.30.379.10. 1 hit.
    InterProi IPR025705. Beta_hexosaminidase_sua/sub.
    IPR008965. Carb-bd_dom.
    IPR012291. CBD_carb-bd_dom.
    IPR004866. CHB/HEX_N_dom.
    IPR004867. CHB_HEX_C_dom.
    IPR029018. Chitobiase/Hex_dom_2-like.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR015883. Glyco_hydro_20_cat-core.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR015882. HEX_bac_N.
    IPR014756. Ig_E-set.
    [Graphical view ]
    Pfami PF03173. CHB_HEX. 1 hit.
    PF03174. CHB_HEX_C. 1 hit.
    PF00728. Glyco_hydro_20. 1 hit.
    PF02838. Glyco_hydro_20b. 1 hit.
    [Graphical view ]
    PRINTSi PR00738. GLHYDRLASE20.
    SMARTi SM01081. CHB_HEX. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49384. SSF49384. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF55545. SSF55545. 1 hit.
    SSF81296. SSF81296. 1 hit.
    PROSITEi PS51257. PROKAR_LIPOPROTEIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "N,N'-diacetylchitobiase of Vibrio harveyi. Primary structure, processing, and evolutionary relationships."
      Soto-Gil R.W., Zysking J.W.
      J. Biol. Chem. 264:14778-14783(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DIACYLGLYCEROL AT CYS-18.

    Entry informationi

    Entry nameiCHB_VIBHA
    AccessioniPrimary (citable) accession number: P13670
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: January 1, 1990
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3