P13669 (MNGR_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mannosyl-D-glycerate transport/metabolism system repressor MngR

Alternative name(s):
Fatty acyl-responsive regulator
Protein P30

Gene names

Name:	mngR
Synonyms:	farR, g30, ybgB
Ordered Locus Names:	b0730, JW0719

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	240 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Represses mngA and mngB. Regulates its own expression. Ref.6 Ref.7
Induction	Induced by mannosyl-D-glycerate.
Disruption phenotype	In cells lacking this gene the expression of mngA and mngB increases by 9-fold and 23-fold, respectively. Ref.7
Sequence similarities	Contains 1 HTH gntR-type DNA-binding domain.
Caution	Originally proposed to be involved in the regulation of the acid citric cycle in response to fatty acids. This was based on in vitro experiments showing that MngR was released from its DNA-binding sites by high concentration sof fatty acids and acyl-CoAs. These results could be explained by the fact that these high-concentrations of fatty acids could have denatured the MngR protein.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of transcription, DNA-dependent Inferred from direct assay Ref.7Ref.6. Source: EcoCyc transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Inferred from direct assay Ref.7Ref.6. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 240	240	Mannosyl-D-glycerate transport/metabolism system repressor MngR		PRO_0000050641
Regions
	Domain	4 – 72	69	HTH gntR-type
	DNA binding	32 – 51	20	H-T-H motif By similarity

Sequences

	Sequence		Length	Mass (Da)
	P13669 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: 69CBB5AFAB2DCDD5	FASTA	240	28,273
	10 20 30 40 50 60 MGHKPLYRQI ADRIREQIAR GELKPGDALP TESALQTEFG VSRVTVRQAL RQLVEQQILE 70 80 90 100 110 120 SIQGSGTYVK EERVNYDIFQ LTSFDEKLSD RHVDTHSEVL IFEVIPADDF LQQQLQITPQ 130 140 150 160 170 180 DRVWHVKRVR YRKQKPMALE ETWMPLALFP DLTWQVMENS KYHFIEEVKK MVIDRSEQEI 190 200 210 220 230 240 IPLMPTEEMS RLLNISQTKP ILEKVSRGYL VDGRVFEYSR NAFNTDDYKF TLIAQRKSSR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Overexpression and site-directed mutagenesis of the succinyl-CoA synthetase of Escherichia coli and nucleotide sequence of a gene (g30) that is adjacent to the suc operon." Buck D., Guest J.R. Biochem. J. 260:737-747(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Isolation and characterization of the heat-responsive genes in Escherichia coli." Utsumi R., Horie T., Katoh A., Kaino Y., Tanabe H., Noda M. Biosci. Biotechnol. Biochem. 60:309-315(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-40.
[5]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 121-240. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Identification of a fatty acyl responsive regulator (FarR) in Escherichia coli." Quail M.A., Dempsey C.E., Guest J.R. FEBS Lett. 356:183-187(1994) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS A REGULATOR OF THE SUCCINYL-COA-SYNTHETASE OPERON.
[7]	"Phosphotransferase-mediated transport of the osmolyte 2-O-alpha-mannosyl-D-glycerate in Escherichia coli occurs by the product of the mngA (hrsA) gene and is regulated by the mngR (farR) gene product acting as repressor." Sampaio M.-M., Chevance F., Dippel R., Eppler T., Schlegel A., Boos W., Lu Y.-J., Rock C.O. J. Biol. Chem. 279:5537-5548(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS A REGULATOR OF THE MANNOSYL-GLYCERATE TRANSPORT AND METABOLISM SYSTEM, DISRUPTION PHENOTYPE. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X15790 Genomic DNA. Translation: CAA33791.1. U00096 Genomic DNA. Translation: AAC73824.1. AP009048 Genomic DNA. Translation: BAA35396.2. D64014 Genomic DNA. No translation available.
PIR	S04645.
RefSeq	NP_415258.1. NC_000913.2. YP_489009.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P13669.
SMR	P13669. Positions 5-238.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-9565N.
IntAct	P13669. 4 interactions.
STRING	511145.b0730.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC73824; AAC73824; b0730. BAA35396; BAA35396; BAA35396.
GeneID	12932231. 945371.
KEGG	ecj:Y75_p0709. eco:b0730.
PATRIC	32116655. VBIEscCol129921_0760.
Organism-specific databases
EchoBASE	EB1100.
EcoGene	EG11109. mngR.
Phylogenomic databases
eggNOG	COG2188.
HOGENOM	HOG000228717.
KO	K11922.
OMA	PCEVNIA.
ProtClustDB	PRK09764.
Enzyme and pathway databases
BioCyc	EcoCyc:PD01103. ECOL316407:JW0719-MONOMER.
Gene expression databases
Genevestigator	P13669.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
InterPro	IPR000524. Tscrpt_reg_HTH_GntR. IPR011663. UTRA. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF00392. GntR. 1 hit. PF07702. UTRA. 1 hit. [Graphical view]
PRINTS	PR00035. HTHGNTR.
SMART	SM00345. HTH_GNTR. 1 hit. SM00866. UTRA. 1 hit. [Graphical view]
PROSITE	PS50949. HTH_GNTR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MNGR_ECOLI

Accession

Primary (citable) accession number: P13669

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	May 1, 2013
This is version 110 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program