Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein disulfide-isomerase A4

Gene

PDIA4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Catalyzes the rearrangement of -S-S- bonds in proteins.

GO - Molecular functioni

  • poly(A) RNA binding Source: UniProtKB
  • protein disulfide isomerase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Enzyme and pathway databases

BioCyciZFISH:HS08062-MONOMER.
BRENDAi5.3.4.1. 2681.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein disulfide-isomerase A4 (EC:5.3.4.1)
Alternative name(s):
Endoplasmic reticulum resident protein 70
Short name:
ER protein 70
Short name:
ERp70
Endoplasmic reticulum resident protein 72
Short name:
ER protein 72
Short name:
ERp-72
Short name:
ERp72
Gene namesi
Name:PDIA4
Synonyms:ERP70, ERP72
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:30167. PDIA4.

Subcellular locationi

GO - Cellular componenti

  • cell surface Source: MGI
  • endoplasmic reticulum lumen Source: ProtInc
  • melanosome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Organism-specific databases

DisGeNETi9601.
OpenTargetsiENSG00000155660.
PharmGKBiPA142671190.

Polymorphism and mutation databases

BioMutaiPDIA4.
DMDMi119530.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000003422921 – 645Protein disulfide-isomerase A4Add BLAST625

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi91 ↔ 94Redox-activePROSITE-ProRule annotation
Disulfide bondi206 ↔ 209Redox-activePROSITE-ProRule annotation
Modified residuei366N6-acetyllysineCombined sources1
Disulfide bondi555 ↔ 558Redox-activePROSITE-ProRule annotation

Keywords - PTMi

Acetylation, Disulfide bond

Proteomic databases

EPDiP13667.
MaxQBiP13667.
PaxDbiP13667.
PeptideAtlasiP13667.
PRIDEiP13667.

2D gel databases

OGPiP13667.
REPRODUCTION-2DPAGEIPI00009904.

PTM databases

iPTMnetiP13667.
PhosphoSitePlusiP13667.
SwissPalmiP13667.

Expressioni

Gene expression databases

BgeeiENSG00000155660.
CleanExiHS_PDIA4.
ExpressionAtlasiP13667. baseline and differential.
GenevisibleiP13667. HS.

Organism-specific databases

HPAiCAB017368.
HPA006139.
HPA006140.

Interactioni

Subunit structurei

Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX.

Binary interactionsi

WithEntry#Exp.IntActNotes
Q763533EBI-1054653,EBI-6248077From a different organism.
PPIBP232843EBI-1054653,EBI-8771982

Protein-protein interaction databases

BioGridi114966. 66 interactors.
IntActiP13667. 26 interactors.
MINTiMINT-4999858.
STRINGi9606.ENSP00000286091.

Structurei

Secondary structure

1645
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi59 – 61Combined sources3
Beta strandi64 – 66Combined sources3
Turni69 – 71Combined sources3
Helixi72 – 76Combined sources5
Beta strandi80 – 87Combined sources8
Helixi92 – 109Combined sources18
Beta strandi111 – 113Combined sources3
Beta strandi117 – 121Combined sources5
Turni122 – 124Combined sources3
Helixi126 – 131Combined sources6
Beta strandi136 – 144Combined sources9
Beta strandi147 – 150Combined sources4
Helixi157 – 168Combined sources12
Beta strandi178 – 181Combined sources4
Turni184 – 186Combined sources3
Helixi187 – 193Combined sources7
Beta strandi195 – 202Combined sources8
Helixi208 – 211Combined sources4
Helixi213 – 224Combined sources12
Beta strandi226 – 228Combined sources3
Beta strandi232 – 236Combined sources5
Turni237 – 239Combined sources3
Helixi241 – 246Combined sources6
Beta strandi251 – 259Combined sources9
Beta strandi262 – 265Combined sources4
Helixi272 – 282Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3IDVX-ray1.95A53-284[»]
ProteinModelPortaliP13667.
SMRiP13667.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP13667.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 169Thioredoxin 1PROSITE-ProRule annotationAdd BLAST149
Domaini158 – 301Thioredoxin 2PROSITE-ProRule annotationAdd BLAST144
Domaini505 – 636Thioredoxin 3PROSITE-ProRule annotationAdd BLAST132

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi642 – 645Prevents secretion from ER4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi39 – 55Asp/Glu-rich (acidic)Add BLAST17

Sequence similaritiesi

Belongs to the protein disulfide isomerase family.Curated
Contains 3 thioredoxin domains.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center, Repeat, Signal

Phylogenomic databases

eggNOGiKOG0190. Eukaryota.
COG0526. LUCA.
GeneTreeiENSGT00860000133691.
HOGENOMiHOG000162459.
HOVERGENiHBG005920.
InParanoidiP13667.
KOiK09582.
OMAiVFKGESD.
OrthoDBiEOG091G05J9.
PhylomeDBiP13667.
TreeFamiTF106382.

Family and domain databases

Gene3Di3.40.30.10. 4 hits.
InterProiIPR005788. Disulphide_isomerase.
IPR005792. Prot_disulphide_isomerase.
IPR017068. Protein_diS-isomerase_A4.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 3 hits.
[Graphical view]
PIRSFiPIRSF036862. Disulphide_isom_A4. 1 hit.
SUPFAMiSSF52833. SSF52833. 5 hits.
TIGRFAMsiTIGR01130. ER_PDI_fam. 1 hit.
TIGR01126. pdi_dom. 3 hits.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 3 hits.
PS51352. THIOREDOXIN_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13667-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPRKAFLLL LLLGLVQLLA VAGAEGPDED SSNRENAIED EEEEEEEDDD
60 70 80 90 100
EEEDDLEVKE ENGVLVLNDA NFDNFVADKD TVLLEFYAPW CGHCKQFAPE
110 120 130 140 150
YEKIANILKD KDPPIPVAKI DATSASVLAS RFDVSGYPTI KILKKGQAVD
160 170 180 190 200
YEGSRTQEEI VAKVREVSQP DWTPPPEVTL VLTKENFDEV VNDADIILVE
210 220 230 240 250
FYAPWCGHCK KLAPEYEKAA KELSKRSPPI PLAKVDATAE TDLAKRFDVS
260 270 280 290 300
GYPTLKIFRK GRPYDYNGPR EKYGIVDYMI EQSGPPSKEI LTLKQVQEFL
310 320 330 340 350
KDGDDVIIIG VFKGESDPAY QQYQDAANNL REDYKFHHTF STEIAKFLKV
360 370 380 390 400
SQGQLVVMQP EKFQSKYEPR SHMMDVQGST QDSAIKDFVL KYALPLVGHR
410 420 430 440 450
KVSNDAKRYT RRPLVVVYYS VDFSFDYRAA TQFWRSKVLE VAKDFPEYTF
460 470 480 490 500
AIADEEDYAG EVKDLGLSES GEDVNAAILD ESGKKFAMEP EEFDSDTLRE
510 520 530 540 550
FVTAFKKGKL KPVIKSQPVP KNNKGPVKVV VGKTFDSIVM DPKKDVLIEF
560 570 580 590 600
YAPWCGHCKQ LEPVYNSLAK KYKGQKGLVI AKMDATANDV PSDRYKVEGF
610 620 630 640
PTIYFAPSGD KKNPVKFEGG DRDLEHLSKF IEEHATKLSR TKEEL
Length:645
Mass (Da):72,932
Last modified:May 1, 1991 - v2
Checksum:i1919C2AE12CD2684
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti102E → G in BAF83660 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_052580173T → M.Corresponds to variant rs2290971dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05016 mRNA. Translation: AAA58460.1.
AK290971 mRNA. Translation: BAF83660.1.
AC093743 Genomic DNA. Translation: AAQ96863.1.
CH471146 Genomic DNA. Translation: EAW80065.1.
CH471146 Genomic DNA. Translation: EAW80066.1.
BC000425 mRNA. Translation: AAH00425.1.
BC001928 mRNA. Translation: AAH01928.1.
BC006344 mRNA. Translation: AAH06344.1.
BC011754 mRNA. Translation: AAH11754.1.
CCDSiCCDS5893.1.
PIRiA23723.
RefSeqiNP_004902.1. NM_004911.4.
UniGeneiHs.93659.

Genome annotation databases

EnsembliENST00000286091; ENSP00000286091; ENSG00000155660.
GeneIDi9601.
KEGGihsa:9601.
UCSCiuc003wff.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05016 mRNA. Translation: AAA58460.1.
AK290971 mRNA. Translation: BAF83660.1.
AC093743 Genomic DNA. Translation: AAQ96863.1.
CH471146 Genomic DNA. Translation: EAW80065.1.
CH471146 Genomic DNA. Translation: EAW80066.1.
BC000425 mRNA. Translation: AAH00425.1.
BC001928 mRNA. Translation: AAH01928.1.
BC006344 mRNA. Translation: AAH06344.1.
BC011754 mRNA. Translation: AAH11754.1.
CCDSiCCDS5893.1.
PIRiA23723.
RefSeqiNP_004902.1. NM_004911.4.
UniGeneiHs.93659.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3IDVX-ray1.95A53-284[»]
ProteinModelPortaliP13667.
SMRiP13667.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114966. 66 interactors.
IntActiP13667. 26 interactors.
MINTiMINT-4999858.
STRINGi9606.ENSP00000286091.

PTM databases

iPTMnetiP13667.
PhosphoSitePlusiP13667.
SwissPalmiP13667.

Polymorphism and mutation databases

BioMutaiPDIA4.
DMDMi119530.

2D gel databases

OGPiP13667.
REPRODUCTION-2DPAGEIPI00009904.

Proteomic databases

EPDiP13667.
MaxQBiP13667.
PaxDbiP13667.
PeptideAtlasiP13667.
PRIDEiP13667.

Protocols and materials databases

DNASUi9601.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000286091; ENSP00000286091; ENSG00000155660.
GeneIDi9601.
KEGGihsa:9601.
UCSCiuc003wff.3. human.

Organism-specific databases

CTDi9601.
DisGeNETi9601.
GeneCardsiPDIA4.
HGNCiHGNC:30167. PDIA4.
HPAiCAB017368.
HPA006139.
HPA006140.
neXtProtiNX_P13667.
OpenTargetsiENSG00000155660.
PharmGKBiPA142671190.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0190. Eukaryota.
COG0526. LUCA.
GeneTreeiENSGT00860000133691.
HOGENOMiHOG000162459.
HOVERGENiHBG005920.
InParanoidiP13667.
KOiK09582.
OMAiVFKGESD.
OrthoDBiEOG091G05J9.
PhylomeDBiP13667.
TreeFamiTF106382.

Enzyme and pathway databases

BioCyciZFISH:HS08062-MONOMER.
BRENDAi5.3.4.1. 2681.

Miscellaneous databases

ChiTaRSiPDIA4. human.
EvolutionaryTraceiP13667.
GenomeRNAii9601.
PROiP13667.

Gene expression databases

BgeeiENSG00000155660.
CleanExiHS_PDIA4.
ExpressionAtlasiP13667. baseline and differential.
GenevisibleiP13667. HS.

Family and domain databases

Gene3Di3.40.30.10. 4 hits.
InterProiIPR005788. Disulphide_isomerase.
IPR005792. Prot_disulphide_isomerase.
IPR017068. Protein_diS-isomerase_A4.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 3 hits.
[Graphical view]
PIRSFiPIRSF036862. Disulphide_isom_A4. 1 hit.
SUPFAMiSSF52833. SSF52833. 5 hits.
TIGRFAMsiTIGR01130. ER_PDI_fam. 1 hit.
TIGR01126. pdi_dom. 3 hits.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 3 hits.
PS51352. THIOREDOXIN_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPDIA4_HUMAN
AccessioniPrimary (citable) accession number: P13667
Secondary accession number(s): A8K4K6, Q549T6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: May 1, 1991
Last modified: November 30, 2016
This is version 189 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Was originally thought to be a deoxycytidine kinase.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.