Reviewed, UniProtKB/Swiss-Prot P13666 (SRCA_RABIT)

Names and origin

Protein names

Recommended name:
    Sarcalumenin

Gene names

Name:

SRL

Organism

Oryctolagus cuniculus (Rabbit)

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	908 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May be involved in the regulation of calcium transport.
Subcellular location	Sarcoplasmic reticulum lumen. Note: Associated through calcium with the membrane.

Ontologies

Keywords
Cellular component	Sarcoplasmic reticulum
Coding sequence diversity	Alternative splicing
Domain	Signal
Ligand	Calcium
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
Cellular component	sarcoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: InterPro GTPase activity Inferred from electronic annotation. Source: InterPro calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P13666-1)

Also known as: 160 kDa;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P13666-2)

Also known as: 58 kDa;

The sequence of this isoform differs from the canonical sequence as follows:
     20-455: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 908	889	Sarcalumenin		PRO_0000022413
Regions
	Region	20 – 457	438	Acidic domain, probably binds calcium
Amino acid modifications
	Modified residue	234	1	Phosphothreonine By similarity
	Glycosylation	102	1	N-linked (GlcNAc...) Potential
	Glycosylation	716	1	N-linked (GlcNAc...) Potential
	Glycosylation	824	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	20 – 455	436	Missing in isoform 2.		VSP_004429
Experimental info
	Sequence conflict	474	1	Q → E in AAA60730. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (160 kDa) [UniParc]. Last modified January 1, 1990. Version 1. Checksum: A48CAA221AE1418B	FASTA	908	97,921
	10 20 30 40 50 60 MRALLLFCFV ASLLLSGQAE LQVPASGGTE DVGNLLENHF SAGDASLEEK ERALYADAAP 70 80 90 100 110 120 RDENLLLHYP DGREAESAER TTAGAPPAAP GPGSEPEASL PNASATESAP PGDATGPREE 130 140 150 160 170 180 QGPAAASALP PGGDEGSLQE ERQELSSGEG PGEEAAGLGL PSEGAASGEM QGQAGGGKVP 190 200 210 220 230 240 KEAEGVLGDS PVQGAAAETA EPEASGIAPS SEDEQIHTLE GEGKGSPGPD HGPTELDGTP 250 260 270 280 290 300 DGASAGEEPK ADLDTEARER AEDQHQVHTL ETEGKGGPGP HQGPAEVDGG PDTVSVGESL 310 320 330 340 350 360 EAREGAEDQG KPSPSQEAGP AAAEAGTEPG GTKHSEEEGM EQDRPPEGQV PVMRQDEGGE 370 380 390 400 410 420 ASSEEEGDEE GGSEEEEGDP SEEDSGEDSG DGASSEEAGA ASEEASGTAG LGEEETQPST 430 440 450 460 470 480 EGLDSGPAGS QAQDTEAEEP EEGHQGPESP ITAPQEETED VSEEVPMRDR SHIQKTLMLN 490 500 510 520 530 540 EDKPTDDFSA VLQRLRKIYH SSIKPLEQSY KYNELRQHEI TDGEITSKPM VLFLGPWSVG 550 560 570 580 590 600 KSTMINYLLG LENTRYQLYT GAEPTTSEFT VLMHGPKLKT IEGIVMAADS ARSFSPLEKF 610 620 630 640 650 660 GQNFLEKLIG IEVPHKLLER VTFVDTPGII ENRKQQERGY PFNDVCQWFI DRADLIFVVF 670 680 690 700 710 720 DPTKLDVGLE LETLFRQLKG RESQIRIILN KADNLATQML MRVYGALFWS LAPLINVTEP 730 740 750 760 770 780 PRVYVSSFWP QEYKPDTHRE LFLREEISLL EDLNQVIENR LENKIAFIRQ HAIRVRIHAL 790 800 810 820 830 840 LVDRYLQTYK DKMTFFSDGE LVFKDIVEDP DKFYIFKTIL AKTNVSKFDL PNREAYKDFF 850 860 870 880 890 900 GINPISSFKL LSQQCSYMGG CFLEKIERAI TQELPSLLGS LGLGKNPGAL NCDKTGCGET PKNRYKKH
	Isoform 2 (58 kDa). Checksum: 71E95D3CD8ABB41A	FASTA	472	54,450
	10 20 30 40 50 60 MRALLLFCFV ASLLLSGQAE ETEDVSEEVP MRDRSHIQKT LMLNEDKPTD DFSAVLQRLR 70 80 90 100 110 120 KIYHSSIKPL EQSYKYNELR QHEITDGEIT SKPMVLFLGP WSVGKSTMIN YLLGLENTRY 130 140 150 160 170 180 QLYTGAEPTT SEFTVLMHGP KLKTIEGIVM AADSARSFSP LEKFGQNFLE KLIGIEVPHK 190 200 210 220 230 240 LLERVTFVDT PGIIENRKQQ ERGYPFNDVC QWFIDRADLI FVVFDPTKLD VGLELETLFR 250 260 270 280 290 300 QLKGRESQIR IILNKADNLA TQMLMRVYGA LFWSLAPLIN VTEPPRVYVS SFWPQEYKPD 310 320 330 340 350 360 THRELFLREE ISLLEDLNQV IENRLENKIA FIRQHAIRVR IHALLVDRYL QTYKDKMTFF 370 380 390 400 410 420 SDGELVFKDI VEDPDKFYIF KTILAKTNVS KFDLPNREAY KDFFGINPIS SFKLLSQQCS 430 440 450 460 470 YMGGCFLEKI ERAITQELPS LLGSLGLGKN PGALNCDKTG CGETPKNRYK KH

References

[1]	"Molecular cloning and expression of cDNA encoding a lumenal calcium binding glycoprotein from sarcoplasmic reticulum." Leberer E., Charuk J.H.M., Green N.M., Maclennan D.H. Proc. Natl. Acad. Sci. U.S.A. 86:6047-6051(1989) [PubMed: 2762314] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Molecular cloning and expression of cDNA encoding the 53,000-dalton glycoprotein of rabbit skeletal muscle sarcoplasmic reticulum." Leberer E., Charuk J.H.M., Clarke D.M., Green N.M., Zubrycka-Gaarn E., McLennan D.H. J. Biol. Chem. 264:3484-3493(1989) [PubMed: 2521635] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).

Cross-references

Sequence databases
EMBL GenBank DDBJ	M25750 mRNA. Translation: AAA31189.1. J04480 mRNA. Translation: AAA60730.1.
PIR	A33280. A33312.
RefSeq	NP_001075750.1.
UniGene	Ocu.595
3D structure databases
SMR	P13666. Positions 490-883.
ModBase	Search...
Genome annotation databases
GeneID	100009112.
Phylogenomic databases
eggNOG	maNOG15181.
HOVERGEN	P13666.
Family and domain databases
InterPro	IPR001401. Dynamin_GTPase. [Graphical view]
Pfam	PF00350. Dynamin_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SRCA_RABIT

Accession

Primary (citable) accession number: P13666

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	January 19, 2010
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)