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Protein

Aspartate-semialdehyde dehydrogenase

Gene

HOM2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the NADPH-dependent formation of L-aspartate-semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl-4-phosphate.By similarity

Catalytic activityi

L-aspartate 4-semialdehyde + phosphate + NADP+ = L-4-aspartyl phosphate + NADPH.

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 2 of the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Aspartokinase (HOM3)
  2. Aspartate-semialdehyde dehydrogenase (HOM2)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes L-homoserine from L-aspartate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Aspartokinase (HOM3)
  2. Aspartate-semialdehyde dehydrogenase (HOM2)
  3. Homoserine dehydrogenase (HOM6)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-homoserine from L-aspartate, the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

Pathwayi: L-threonine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-threonine from L-aspartate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Aspartokinase (HOM3)
  2. Aspartate-semialdehyde dehydrogenase (HOM2)
  3. Homoserine dehydrogenase (HOM6)
  4. Homoserine kinase (THR1)
  5. Threonine synthase (THR4)
This subpathway is part of the pathway L-threonine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-threonine from L-aspartate, the pathway L-threonine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei112PhosphateBy similarity1
Active sitei156Acyl-thioester intermediateBy similarity1
Binding sitei184SubstrateBy similarity1
Binding sitei211SubstrateBy similarity1
Binding sitei214PhosphateBy similarity1
Binding sitei249SubstrateBy similarity1
Active sitei256Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi13 – 16NADPBy similarity4
Nucleotide bindingi40 – 41NADPBy similarity2
Nucleotide bindingi187 – 188NADPBy similarity2
Nucleotide bindingi343 – 344NADPBy similarity2

GO - Molecular functioni

GO - Biological processi

  • aspartate metabolic process Source: InterPro
  • homoserine biosynthetic process Source: SGD
  • isoleucine biosynthetic process Source: InterPro
  • lysine biosynthetic process via diaminopimelate Source: UniProtKB-UniPathway
  • methionine biosynthetic process Source: SGD
  • threonine biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Lysine biosynthesis, Methionine biosynthesis, Threonine biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciYEAST:YDR158W-MONOMER.
UniPathwayiUPA00034; UER00016.
UPA00050; UER00463.
UPA00051; UER00464.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate-semialdehyde dehydrogenase (EC:1.2.1.11)
Short name:
ASA dehydrogenase
Short name:
ASADH
Alternative name(s):
Aspartate-beta-semialdehyde dehydrogenase
Gene namesi
Name:HOM2
Ordered Locus Names:YDR158W
ORF Names:YD8358.12
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR158W.
SGDiS000002565. HOM2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001414001 – 365Aspartate-semialdehyde dehydrogenaseAdd BLAST365

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei13PhosphothreonineCombined sources1
Modified residuei318PhosphoserineCombined sources1
Modified residuei323PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP13663.
PRIDEiP13663.

2D gel databases

SWISS-2DPAGEP13663.

PTM databases

iPTMnetiP13663.

Expressioni

Inductioni

By high concentrations of Met (general AA biosynthesis control).

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi32209. 138 interactors.
DIPiDIP-5204N.
IntActiP13663. 17 interactors.
MINTiMINT-535737.

Structurei

3D structure databases

ProteinModelPortaliP13663.
SMRiP13663.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000013358.
InParanoidiP13663.
KOiK00133.
OMAiASCHRVP.
OrthoDBiEOG092C309U.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_02121. ASADH. 1 hit.
InterProiIPR000319. Asp-semialdehyde_DH_CS.
IPR005676. Asp_semi-ald_DH_pep-lack.
IPR012080. Asp_semialdehyde_DH.
IPR016040. NAD(P)-bd_dom.
IPR000534. Semialdehyde_DH_NAD-bd.
IPR012280. Semialdhyde_DH_dimer_dom.
[Graphical view]
PfamiPF01118. Semialdhyde_dh. 1 hit.
PF02774. Semialdhyde_dhC. 1 hit.
[Graphical view]
PIRSFiPIRSF000148. ASA_dh. 1 hit.
SMARTiSM00859. Semialdhyde_dh. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00978. asd_EA. 1 hit.
PROSITEiPS01103. ASD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P13663-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGKKIAGVL GATGSVGQRF ILLLANHPHF ELKVLGASSR SAGKKYVDAV
60 70 80 90 100
NWKQTDLLPE SATDIIVSEC KSEFFKECDI VFSGLDADYA GAIEKEFMEA
110 120 130 140 150
GIAIVSNAKN YRREQDVPLI VPVVNPEHLD IVAQKLDTAK AQGKPRPGFI
160 170 180 190 200
ICISNCSTAG LVAPLKPLIE KFGPIDALTT TTLQAISGAG FSPGVPGIDI
210 220 230 240 250
LDNIIPYIGG EEDKMEWETK KILAPLAEDK THVKLLTPEE IKVSAQCNRV
260 270 280 290 300
AVSDGHTECI SLRFKNRPAP SVEQVKTCLK EYVCDAYKLG CHSAPKQTIH
310 320 330 340 350
VLEQPDRPQP RLDRNRDSGY GVSVGRIRED PLLDFKMVVL SHNTIIGAAG
360
SGVLIAEILL ARNLI
Length:365
Mass (Da):39,544
Last modified:January 1, 1990 - v1
Checksum:iD33096DA8C2B6424
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15649 Genomic DNA. Translation: CAA33675.1.
Z50046 Genomic DNA. Translation: CAA90378.1.
AY557698 Genomic DNA. Translation: AAS56024.1.
BK006938 Genomic DNA. Translation: DAA11998.1.
PIRiJQ0198.
RefSeqiNP_010442.3. NM_001180465.3.

Genome annotation databases

EnsemblFungiiYDR158W; YDR158W; YDR158W.
GeneIDi851736.
KEGGisce:YDR158W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15649 Genomic DNA. Translation: CAA33675.1.
Z50046 Genomic DNA. Translation: CAA90378.1.
AY557698 Genomic DNA. Translation: AAS56024.1.
BK006938 Genomic DNA. Translation: DAA11998.1.
PIRiJQ0198.
RefSeqiNP_010442.3. NM_001180465.3.

3D structure databases

ProteinModelPortaliP13663.
SMRiP13663.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32209. 138 interactors.
DIPiDIP-5204N.
IntActiP13663. 17 interactors.
MINTiMINT-535737.

PTM databases

iPTMnetiP13663.

2D gel databases

SWISS-2DPAGEP13663.

Proteomic databases

MaxQBiP13663.
PRIDEiP13663.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR158W; YDR158W; YDR158W.
GeneIDi851736.
KEGGisce:YDR158W.

Organism-specific databases

EuPathDBiFungiDB:YDR158W.
SGDiS000002565. HOM2.

Phylogenomic databases

HOGENOMiHOG000013358.
InParanoidiP13663.
KOiK00133.
OMAiASCHRVP.
OrthoDBiEOG092C309U.

Enzyme and pathway databases

UniPathwayiUPA00034; UER00016.
UPA00050; UER00463.
UPA00051; UER00464.
BioCyciYEAST:YDR158W-MONOMER.

Miscellaneous databases

PROiP13663.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_02121. ASADH. 1 hit.
InterProiIPR000319. Asp-semialdehyde_DH_CS.
IPR005676. Asp_semi-ald_DH_pep-lack.
IPR012080. Asp_semialdehyde_DH.
IPR016040. NAD(P)-bd_dom.
IPR000534. Semialdehyde_DH_NAD-bd.
IPR012280. Semialdhyde_DH_dimer_dom.
[Graphical view]
PfamiPF01118. Semialdhyde_dh. 1 hit.
PF02774. Semialdhyde_dhC. 1 hit.
[Graphical view]
PIRSFiPIRSF000148. ASA_dh. 1 hit.
SMARTiSM00859. Semialdhyde_dh. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00978. asd_EA. 1 hit.
PROSITEiPS01103. ASD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDHAS_YEAST
AccessioniPrimary (citable) accession number: P13663
Secondary accession number(s): D6VSD8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: November 2, 2016
This is version 160 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 18400 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.