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P13661

- BLO1_ECOLX

UniProt

P13661 - BLO1_ECOLX

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Protein

Beta-lactamase OXA-1

Gene

bla

Organism
Escherichia coli
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

This is an oxacillin-hydrolyzing beta-lactamase.

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei71 – 711Acyl-ester intermediate

GO - Molecular functioni

  1. beta-lactamase activity Source: UniProtKB-EC
  2. penicillin binding Source: InterPro

GO - Biological processi

  1. antibiotic catabolic process Source: InterPro
  2. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Enzyme and pathway databases

SABIO-RKP13661.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase OXA-1 (EC:3.5.2.6)
Alternative name(s):
Penicillinase
Gene namesi
Name:bla
Synonyms:oxa1
Encoded oniPlasmid RGN2380 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 25251 PublicationAdd
BLAST
Chaini26 – 276251Beta-lactamase OXA-1PRO_0000017024Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei74 – 741N6-carboxylysine1 Publication

Interactioni

Subunit structurei

Monomer.1 Publication

Structurei

Secondary structure

1
276
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi30 – 378Combined sources
Beta strandi40 – 489Combined sources
Turni49 – 513Combined sources
Beta strandi54 – 585Combined sources
Helixi60 – 634Combined sources
Helixi70 – 723Combined sources
Helixi73 – 8311Combined sources
Helixi103 – 1053Combined sources
Helixi111 – 1177Combined sources
Helixi120 – 13011Combined sources
Helixi132 – 14211Combined sources
Turni153 – 1553Combined sources
Helixi158 – 1614Combined sources
Beta strandi164 – 1674Combined sources
Helixi172 – 18312Combined sources
Beta strandi187 – 1893Combined sources
Helixi191 – 20111Combined sources
Beta strandi202 – 2054Combined sources
Beta strandi211 – 22010Combined sources
Beta strandi227 – 23610Combined sources
Beta strandi242 – 25110Combined sources
Helixi259 – 27315Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1M6KX-ray1.50A/B26-276[»]
3ISGX-ray1.40A/B26-276[»]
4MLLX-ray1.37A/B/C/D26-276[»]
ProteinModelPortaliP13661.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP13661.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni238 – 2403Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the class-D beta-lactamase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR002137. Beta-lactam_class-D_AS.
IPR001460. PCN-bd_Tpept.
[Graphical view]
PfamiPF00905. Transpeptidase. 1 hit.
[Graphical view]
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00337. BETA_LACTAMASE_D. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13661-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKNTIHINFA IFLIIANIIY SSASASTDIS TVASPLFEGT EGCFLLYDAS
60 70 80 90 100
TNAEIAQFNK AKCATQMAPD STFKIALSLM AFDAEIIDQK TIFKWDKTPK
110 120 130 140 150
GMEIWNSNHT PKTWMQFSVV WVSQEITQKI GLNKIKNYLK DFDYGNQDFS
160 170 180 190 200
GDKERNNGLT EAWLESSLKI SPEEQIQFLR KIINHNLPVK NSAIENTIEN
210 220 230 240 250
MYLQDLDNST KLYGKTGAGF TANRTLQNGW FEGFIISKSG HKYVFVSALT
260 270
GNLGSNLTSS IKAKKNAITI LNTLNL
Length:276
Mass (Da):30,880
Last modified:February 10, 2009 - v2
Checksum:i5ADF7626422BA124
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02967 Genomic DNA. Translation: AAA91586.2.
PIRiA39880.
RefSeqiNP_957554.1. NC_005327.1.
YP_006953880.1. NC_019089.1.
YP_008574821.1. NC_022374.1.

Genome annotation databases

GeneIDi13906817.
17035637.
2716479.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02967 Genomic DNA. Translation: AAA91586.2 .
PIRi A39880.
RefSeqi NP_957554.1. NC_005327.1.
YP_006953880.1. NC_019089.1.
YP_008574821.1. NC_022374.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1M6K X-ray 1.50 A/B 26-276 [» ]
3ISG X-ray 1.40 A/B 26-276 [» ]
4MLL X-ray 1.37 A/B/C/D 26-276 [» ]
ProteinModelPortali P13661.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi P13661.
ChEMBLi CHEMBL4951.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 13906817.
17035637.
2716479.

Enzyme and pathway databases

SABIO-RK P13661.

Miscellaneous databases

EvolutionaryTracei P13661.

Family and domain databases

Gene3Di 3.40.710.10. 1 hit.
InterProi IPR012338. Beta-lactam/transpept-like.
IPR002137. Beta-lactam_class-D_AS.
IPR001460. PCN-bd_Tpept.
[Graphical view ]
Pfami PF00905. Transpeptidase. 1 hit.
[Graphical view ]
SUPFAMi SSF56601. SSF56601. 1 hit.
PROSITEi PS00337. BETA_LACTAMASE_D. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Precise insertion of antibiotic resistance determinants into Tn21-like transposons: nucleotide sequence of the OXA-1 beta-lactamase gene."
    Ouellette M., Bissonnette L., Roy P.H.
    Proc. Natl. Acad. Sci. U.S.A. 84:7378-7382(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Transposon: Tn2603.
  2. Ouellette M., Roy P.H.
    Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 131.
  3. "Comparison of beta-lactamases of classes A and D: 1.5-A crystallographic structure of the class D OXA-1 oxacillinase."
    Sun T., Nukaga M., Mayama K., Braswell E.H., Knox J.R.
    Protein Sci. 12:82-91(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 26-276, PROTEIN SEQUENCE OF N-TERMINUS, CARBAMYLATION AT LYS-74, SUBUNIT.

Entry informationi

Entry nameiBLO1_ECOLX
AccessioniPrimary (citable) accession number: P13661
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: February 10, 2009
Last modified: November 26, 2014
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Plasmid, Transposable element

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3