Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P13661 (BLO1_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-lactamase OXA-1

EC=3.5.2.6
Alternative name(s):
Penicillinase
Gene names
Name:bla
Synonyms:oxa1
Encoded onPlasmid RGN238
OrganismEscherichia coli
Taxonomic identifier562 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length276 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This is an oxacillin-hydrolyzing beta-lactamase.

Catalytic activity

A beta-lactam + H2O = a substituted beta-amino acid.

Subunit structure

Monomer. Ref.3

Sequence similarities

Belongs to the class-D beta-lactamase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Ref.3
Chain26 – 276251Beta-lactamase OXA-1
PRO_0000017024

Regions

Region238 – 2403Substrate binding By similarity

Sites

Active site711Acyl-ester intermediate

Amino acid modifications

Modified residue741N6-carboxylysine

Secondary structure

.......................................... 276
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P13661 [UniParc].

Last modified February 10, 2009. Version 2.
Checksum: 5ADF7626422BA124

FASTA27630,880
        10         20         30         40         50         60 
MKNTIHINFA IFLIIANIIY SSASASTDIS TVASPLFEGT EGCFLLYDAS TNAEIAQFNK 

        70         80         90        100        110        120 
AKCATQMAPD STFKIALSLM AFDAEIIDQK TIFKWDKTPK GMEIWNSNHT PKTWMQFSVV 

       130        140        150        160        170        180 
WVSQEITQKI GLNKIKNYLK DFDYGNQDFS GDKERNNGLT EAWLESSLKI SPEEQIQFLR 

       190        200        210        220        230        240 
KIINHNLPVK NSAIENTIEN MYLQDLDNST KLYGKTGAGF TANRTLQNGW FEGFIISKSG 

       250        260        270 
HKYVFVSALT GNLGSNLTSS IKAKKNAITI LNTLNL 

« Hide

References

[1]"Precise insertion of antibiotic resistance determinants into Tn21-like transposons: nucleotide sequence of the OXA-1 beta-lactamase gene."
Ouellette M., Bissonnette L., Roy P.H.
Proc. Natl. Acad. Sci. U.S.A. 84:7378-7382(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Transposon: Tn2603.
[2]Ouellette M., Roy P.H.
Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO 131.
[3]"Comparison of beta-lactamases of classes A and D: 1.5-A crystallographic structure of the class D OXA-1 oxacillinase."
Sun T., Nukaga M., Mayama K., Braswell E.H., Knox J.R.
Protein Sci. 12:82-91(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 26-276, PROTEIN SEQUENCE OF N-TERMINUS, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J02967 Genomic DNA. Translation: AAA91586.2.
PIRA39880.
RefSeqNP_957554.1. NC_005327.1.
YP_006953880.1. NC_019089.1.
YP_008574821.1. NC_022374.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1M6KX-ray1.50A/B26-276[»]
3ISGX-ray1.40A/B26-276[»]
4MLLX-ray1.37A/B/C/D26-276[»]
ProteinModelPortalP13661.
ModBaseSearch...
MobiDBSearch...

Chemistry

ChEMBLCHEMBL4951.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID13906817.
17035637.
2716479.

Enzyme and pathway databases

SABIO-RKP13661.

Family and domain databases

Gene3D3.40.710.10. 1 hit.
InterProIPR012338. Beta-lactam/transpept-like.
IPR002137. Beta-lactam_class-D_AS.
IPR001460. PCN-bd_Tpept.
[Graphical view]
PfamPF00905. Transpeptidase. 1 hit.
[Graphical view]
SUPFAMSSF56601. SSF56601. 1 hit.
PROSITEPS00337. BETA_LACTAMASE_D. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP13661.

Entry information

Entry nameBLO1_ECOLX
AccessionPrimary (citable) accession number: P13661
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: February 10, 2009
Last modified: May 14, 2014
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references