##gff-version 3
P13658	UniProtKB	Chain	1	517	.	.	.	ID=PRO_0000068400;Note=DNA relaxase MbeA	
P13658	UniProtKB	Region	281	310	.	.	.	Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite	
P13658	UniProtKB	Region	380	405	.	.	.	Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite	
P13658	UniProtKB	Region	496	517	.	.	.	Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite	
P13658	UniProtKB	Compositional bias	294	310	.	.	.	Note=Basic and acidic residues;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite	
P13658	UniProtKB	Active site	19	19	.	.	.	Note=O-(5'-phospho-DNA)-tyrosine intermediate	
P13658	UniProtKB	Binding site	97	97	.	.	.	Ontology_term=ECO:0000305;evidence=ECO:0000305	
P13658	UniProtKB	Binding site	104	104	.	.	.	Ontology_term=ECO:0000305;evidence=ECO:0000305	
P13658	UniProtKB	Binding site	106	106	.	.	.	Ontology_term=ECO:0000305;evidence=ECO:0000305	
P13658	UniProtKB	Mutagenesis	19	19	.	.	.	Note=Loss of in vitro DNA-cleavage activity and in vivo functionality. Y->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12675806;Dbxref=PMID:12675806	
P13658	UniProtKB	Mutagenesis	97	97	.	.	.	Note=Loss of in vitro DNA-cleavage activity and in vivo functionality. No effect on DNA binding around the nic site. H->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12675806;Dbxref=PMID:12675806	
P13658	UniProtKB	Mutagenesis	104	104	.	.	.	Note=Loss of in vitro DNA-cleavage activity and in vivo functionality. No effect on DNA binding around the nic site. E->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12675806;Dbxref=PMID:12675806	
P13658	UniProtKB	Mutagenesis	104	104	.	.	.	Note=Loss of the in vitro DNA-cleavage and strand-transfer activities but displays 1%25 of the in vivo wild-type activity%3B when associated with H-106. E->H;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12675806;Dbxref=PMID:12675806	
P13658	UniProtKB	Mutagenesis	106	106	.	.	.	Note=4-fold and 3-fold reduction in the in vitro DNA-cleavage and strand-transfer activities%2C respectively%2C but no reduction in the in vivo functionality. N->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12675806;Dbxref=PMID:12675806	
P13658	UniProtKB	Mutagenesis	106	106	.	.	.	Note=Loss of the in vitro DNA-cleavage and strand-transfer activities but displays 1%25 of the in vivo wild-type activity%3B when associated with H-104. N->H;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12675806;Dbxref=PMID:12675806