Reviewed,
UniProtKB/Swiss-Prot P13656 (CHIA_ECOLI)
Last modified
June 16, 2009.
Version 88.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Probable bifunctional chitinase/lysozyme Including the following 2 domains: 1- Recommended name: Chitinase EC=3.2.1.14 2- Recommended name: Lysozyme EC=3.2.1.17 | ||||||
| Gene names |
| ||||||
| Organism | Escherichia coli (strain K12) [Complete proteome] [HAMAP] | ||||||
| Taxonomic identifier | 83333 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia |
Protein attributes
| Sequence length | 897 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Bifunctional enzyme with lysozyme/chitinase activity. |
| Catalytic activity | Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins. Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins. |
| Subcellular location | |
| Miscellaneous | In E.coli K12, chiA expression is repressed. The DNA-binding protein H-NS participates in the silencing of chiA. |
| Sequence similarities | Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily. Contains 5 chitin-binding type-3 domains. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Carbohydrate metabolism Chitin degradation Polysaccharide degradation |
| Cellular component | Periplasm |
| Domain | Repeat Signal |
| Ligand | Chitin-binding |
| Molecular function | Glycosidase Hydrolase |
| PTM | Disulfide bond |
| Technical term | Complete proteome Multifunctional enzyme |
| Gene Ontology (GO) | |
| Biological process | chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW polysaccharide catabolic processInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | extracellular region Inferred from electronic annotation. Source: InterPro periplasmic spaceInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | chitin binding Inferred from electronic annotation. Source: UniProtKB-KW chitinase activityInferred from electronic annotation. Source: EC lysozyme activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 24 | 24 | Potential | ||||||||
| Chain | 25 – 897 | 873 | Probable bifunctional chitinase/lysozyme | PRO_0000011907 | |||||||
Regions | |||||||||||
| Domain | 25 – 91 | 67 | Chitin-binding type-3 1 | ||||||||
| Domain | 128 – 194 | 67 | Chitin-binding type-3 2 | ||||||||
| Domain | 229 – 295 | 67 | Chitin-binding type-3 3 | ||||||||
| Domain | 337 – 403 | 67 | Chitin-binding type-3 4 | ||||||||
| Domain | 459 – 529 | 71 | Chitin-binding type-3 5 | ||||||||
| Region | 647 – 897 | 251 | Catalytic By similarity | ||||||||
| Compositional bias | 93 – 101 | 9 | Poly-Ser | ||||||||
Sites | |||||||||||
| Active site | 700 | 1 | Proton donor By similarity | ||||||||
Amino acid modifications | |||||||||||
| Disulfide bond | 628 ↔ 673 | By similarity | |||||||||
Experimental info | |||||||||||
| Sequence conflict | 874 | 1 | F → I in AAC13985. Ref.3 | ||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076. |
| [2] | "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
| [3] | "Cloning, sequencing, and mapping of the bacterioferritin gene (bfr) of Escherichia coli K-12." Andrews S.C., Harrison P.M., Guest J.R. J. Bacteriol. 171:3940-3947(1989) [PubMed: 2661540] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 874-897. Strain: K12. |
| [4] | "Functional analysis of the carbohydrate-binding domains of Erwinia chrysanthemi Cel5 (Endoglucanase Z) and an Escherichia coli putative chitinase." Simpson H.D., Barras F. J. Bacteriol. 181:4611-4616(1999) [PubMed: 10419961] [Abstract] Cited for: CHITIN-BINDING PROPERTIES. |
| [5] | "The ChiA (YheB) protein of Escherichia coli K-12 is an endochitinase whose gene is negatively controlled by the nucleoid-structuring protein H-NS." Francetic O., Badaut C., Rimsky S., Pugsley A.P. Mol. Microbiol. 35:1506-1517(2000) [PubMed: 10760150] [Abstract] Cited for: CHARACTERIZATION. Strain: K12 / MG1655 / ATCC 47076. |
Cross-references
Sequence databases | |
|---|---|
| U18997 Genomic DNA. Translation: AAA58135.1. U00096 Genomic DNA. Translation: AAC76363.1. AP009048 Genomic DNA. Translation: BAE77953.1. M27176 mRNA. Translation: AAC13985.1. | |
| PIR | E65127. |
| RefSeq | AP_004452.1. NP_417797.1. |
3D structure databases | |
| ModBase | Search... |
Protein family/group databases | |
| CAZy | CBM5. Carbohydrate-Binding Module Family 5. GH18. Glycoside Hydrolase Family 18. |
Genome annotation databases | |
| GeneID | 947837. |
| GenomeReviews | Gene locus JW3300 in contig AP009048_GR. Gene locus b3338 in contig U00096_GR. |
| KEGG | ecj:JW3300. eco:b3338. |
Organism-specific databases | |
| EchoBASE | EB1219. |
| EcoGene | EG11237. chiA. |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | P13656. |
| OMA | P13656. YKNAWWV. |
Enzyme and pathway databases | |
| BioCyc | EcoCyc:EG11237-MON. |
Family and domain databases | |
| InterPro | IPR003610. CBM_5_12. IPR001579. Glyco_hydro_18_chit_AS. [Graphical view] |
| Pfam | PF02839. CBM_5_12. 1 hit. [Graphical view] |
| SMART | SM00495. ChtBD3. 7 hits. [Graphical view] |
| PROSITE | PS01095. CHITINASE_18. False negative. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | CHIA_ECOLI | ||||||||
| Accession | Primary (citable) accession number: P13656 Secondary accession number(s): Q2M703 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Escherichia coli Escherichia coli (strain K12): entries and cross-references to EcoGene |
| Glycosyl hydrolases Classification of glycosyl hydrolase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with


