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P13656

- CHIA_ECOLI

UniProt

P13656 - CHIA_ECOLI

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Protein

Probable bifunctional chitinase/lysozyme

Gene

chiA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Bifunctional enzyme with lysozyme/chitinase activity.1 Publication

Catalytic activityi

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.1 Publication
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei700 – 7001Proton donorBy similarity

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. chitin binding Source: UniProtKB-KW
  3. endochitinase activity Source: EcoCyc
  4. lysozyme activity Source: UniProtKB-EC

GO - Biological processi

  1. chitin catabolic process Source: UniProtKB-KW
  2. polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Chitin degradation, Polysaccharide degradation

Keywords - Ligandi

Chitin-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11237-MONOMER.
ECOL316407:JW3300-MONOMER.
MetaCyc:EG11237-MONOMER.

Protein family/group databases

CAZyiCBM5. Carbohydrate-Binding Module Family 5.
GH18. Glycoside Hydrolase Family 18.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable bifunctional chitinase/lysozyme
Including the following 2 domains:
Chitinase (EC:3.2.1.14)
Lysozyme (EC:3.2.1.17)
Gene namesi
Name:chiA
Synonyms:yheB
Ordered Locus Names:b3338, JW3300
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG11237. chiA.

Subcellular locationi

Periplasm 1 Publication
Note: Secreted via the Gsp type II secretion machinery under conditions of derepressed gsp gene expression.

GO - Cellular componenti

  1. extracellular region Source: InterPro
  2. outer membrane-bounded periplasmic space Source: EcoCyc
  3. periplasmic space Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Chaini25 – 897873Probable bifunctional chitinase/lysozymePRO_0000011907Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi628 ↔ 673By similarity

Keywords - PTMi

Disulfide bond

Expressioni

Inductioni

Silenced by the DNA-binding protein H-NS under standard growth conditions.1 Publication

Gene expression databases

GenevestigatoriP13656.

Interactioni

Protein-protein interaction databases

DIPiDIP-9276N.
IntActiP13656. 1 interaction.
STRINGi511145.b3338.

Structurei

3D structure databases

ProteinModelPortaliP13656.
SMRiP13656. Positions 585-896.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 9167Chitin-binding type-3 1Add
BLAST
Domaini128 – 19467Chitin-binding type-3 2Add
BLAST
Domaini229 – 29567Chitin-binding type-3 3Add
BLAST
Domaini337 – 40367Chitin-binding type-3 4Add
BLAST
Domaini459 – 52971Chitin-binding type-3 5Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni647 – 897251CatalyticBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi93 – 1019Poly-Ser

Sequence similaritiesi

Contains 5 chitin-binding type-3 domains.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG12793.
HOGENOMiHOG000125409.
InParanoidiP13656.
KOiK13381.
OMAiGSIYKNA.
OrthoDBiEOG6QVRC6.

Family and domain databases

Gene3Di2.10.10.20. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR003610. CBM_fam5/12.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02839. CBM_5_12. 1 hit.
[Graphical view]
SMARTiSM00495. ChtBD3. 7 hits.
[Graphical view]
SUPFAMiSSF51055. SSF51055. 1 hit.
SSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13656-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKLNIFTKSM IGMGLVCSAL PALAMEAWNN QQGGNKYQVI FDGKIYENAW
60 70 80 90 100
WVSSTNCPGK AKANDATNPW RLKRTATAAE ISQFGNTLSC EKSGSSSSSN
110 120 130 140 150
SNTPASNTPA NGGSATPAQG TVPSNSSVVA WNKQQGGQTW YVVFNGAVYK
160 170 180 190 200
NAWWVASSNC PGDAKSNDAS NPWRYVRAAT ATEISETSNP QSCTSAPQPS
210 220 230 240 250
PDVKPAPDVK PAPDVQPAPA DKSNDNYAVV AWKGQEGSST WYVIYNGGIY
260 270 280 290 300
KNAWWVGAAN CPGDAKENDA SNPWRYVRAA TATEISQYGN PGSCSVKPDN
310 320 330 340 350
NGGAVTPVDP TPETPVTPTP DNSEPSTPAD SVNDYSLQAW SGQEGSEIYH
360 370 380 390 400
VIFNGNVYKN AWWVGSKDCP RGTSAENSNN PWRLERTATA AELSQYGNPT
410 420 430 440 450
TCEIDNGGVI VADGFQASKA YSADSIVDYN DAHYKTSVDQ DAWGFVPGGD
460 470 480 490 500
NPWKKYEPAK AWSASTVYVK GDRVVVDGQA YEALFWTQSD NPALVANQNA
510 520 530 540 550
TGSNSRPWKP LGKAQSYSNE ELNNAPQFNP ETLYASDTLI RFNGVNYISQ
560 570 580 590 600
SKVQKVSPSD SNPWRVFVDW TGTKERVGTP KKAWPKHVYA PYVDFTLNTI
610 620 630 640 650
PDLAALAKNH NVNHFTLAFV VSKDANTCLP TWGTAYGMQN YAQYSKIKAL
660 670 680 690 700
REAGGDVMLS IGGANNAPLA ASCKNVDDLM QHYYDIVDNL NLKVLDFDIE
710 720 730 740 750
GTWVADQASI ERRNLAVKKV QDKWKSEGKD IAIWYTLPIL PTGLTPEGMN
760 770 780 790 800
VLSDAKAKGV ELAGVNVMTM DYGNAICQSA NTEGQNIHGK CATSAIANLH
810 820 830 840 850
SQLKGLHPNK SDAEIDAMMG TTPMVGVNDV QGEVFYLSDA RLVMQDAQKR
860 870 880 890
NLGMVGIWSI ARDLPGGTNL SPEFHGLTKE QAPKYAFSEI FAPFTKQ
Length:897
Mass (Da):97,058
Last modified:November 1, 1995 - v2
Checksum:i968D145BA1F954F3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti874 – 8741F → I in AAC13985. (PubMed:2661540)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18997 Genomic DNA. Translation: AAA58135.1.
U00096 Genomic DNA. Translation: AAC76363.1.
AP009048 Genomic DNA. Translation: BAE77953.1.
M27176 mRNA. Translation: AAC13985.1.
PIRiE65127.
RefSeqiNP_417797.1. NC_000913.3.
YP_492094.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC76363; AAC76363; b3338.
BAE77953; BAE77953; BAE77953.
GeneIDi12932022.
947837.
KEGGiecj:Y75_p3838.
eco:b3338.
PATRICi32122108. VBIEscCol129921_3431.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18997 Genomic DNA. Translation: AAA58135.1 .
U00096 Genomic DNA. Translation: AAC76363.1 .
AP009048 Genomic DNA. Translation: BAE77953.1 .
M27176 mRNA. Translation: AAC13985.1 .
PIRi E65127.
RefSeqi NP_417797.1. NC_000913.3.
YP_492094.1. NC_007779.1.

3D structure databases

ProteinModelPortali P13656.
SMRi P13656. Positions 585-896.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-9276N.
IntActi P13656. 1 interaction.
STRINGi 511145.b3338.

Protein family/group databases

CAZyi CBM5. Carbohydrate-Binding Module Family 5.
GH18. Glycoside Hydrolase Family 18.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC76363 ; AAC76363 ; b3338 .
BAE77953 ; BAE77953 ; BAE77953 .
GeneIDi 12932022.
947837.
KEGGi ecj:Y75_p3838.
eco:b3338.
PATRICi 32122108. VBIEscCol129921_3431.

Organism-specific databases

EchoBASEi EB1219.
EcoGenei EG11237. chiA.

Phylogenomic databases

eggNOGi NOG12793.
HOGENOMi HOG000125409.
InParanoidi P13656.
KOi K13381.
OMAi GSIYKNA.
OrthoDBi EOG6QVRC6.

Enzyme and pathway databases

BioCyci EcoCyc:EG11237-MONOMER.
ECOL316407:JW3300-MONOMER.
MetaCyc:EG11237-MONOMER.

Miscellaneous databases

PROi P13656.

Gene expression databases

Genevestigatori P13656.

Family and domain databases

Gene3Di 2.10.10.20. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR003610. CBM_fam5/12.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02839. CBM_5_12. 1 hit.
[Graphical view ]
SMARTi SM00495. ChtBD3. 7 hits.
[Graphical view ]
SUPFAMi SSF51055. SSF51055. 1 hit.
SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Cloning, sequencing, and mapping of the bacterioferritin gene (bfr) of Escherichia coli K-12."
    Andrews S.C., Harrison P.M., Guest J.R.
    J. Bacteriol. 171:3940-3947(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 874-897.
    Strain: K12.
  4. "Functional analysis of the carbohydrate-binding domains of Erwinia chrysanthemi Cel5 (Endoglucanase Z) and an Escherichia coli putative chitinase."
    Simpson H.D., Barras F.
    J. Bacteriol. 181:4611-4616(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHITIN-BINDING PROPERTIES.
  5. "The ChiA (YheB) protein of Escherichia coli K-12 is an endochitinase whose gene is negatively controlled by the nucleoid-structuring protein H-NS."
    Francetic O., Badaut C., Rimsky S., Pugsley A.P.
    Mol. Microbiol. 35:1506-1517(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, TRANSCRIPTIONAL REGULATION, GENE NAME.
    Strain: K12 / MG1655 / ATCC 47076.
  6. "Expression of the endogenous type II secretion pathway in Escherichia coli leads to chitinase secretion."
    Francetic O., Belin D., Badaut C., Pugsley A.P.
    EMBO J. 19:6697-6703(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: SECRETION VIA THE GSP SECRETON.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.

Entry informationi

Entry nameiCHIA_ECOLI
AccessioniPrimary (citable) accession number: P13656
Secondary accession number(s): Q2M703
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: November 1, 1995
Last modified: October 29, 2014
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3