Reviewed, UniProtKB/Swiss-Prot P13653 (PORA_HORVU)

Names and origin

Protein names

Recommended name:
    Protochlorophyllide reductase A, chloroplastic
      Short name=PCR A
    EC=1.3.1.33
Alternative name(s):
    NADPH-protochlorophyllide oxidoreductase A
      Short name=POR A

Gene names

Name:

PORA

Organism

Hordeum vulgare (Barley)

Taxonomic identifier

4513 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Phototransformation of protochlorophyllide (Pchlide) to chlorophyllide (Chlide).
Catalytic activity	Chlorophyllide a + NADP(+) = protochlorophyllide + NADPH.
Pathway	Porphyrin biosynthesis; chlorophyll biosynthesis.
Subcellular location	Plastid › chloroplast.
Developmental stage	Active only transiently in etiolated seedlings at the beginning of illumination.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. POR subfamily.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Photosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis, dark reaction Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast Inferred from electronic annotation. Source: InterPro
Molecular function	binding Inferred from electronic annotation. Source: InterPro protochlorophyllide reductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 74	74	Chloroplast
	Chain	75 – 388	314	Protochlorophyllide reductase A, chloroplastic		PRO_0000023293

Sequences

	Sequence		Length	Mass (Da)
	P13653-1 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: EBD3EF153D96C129	FASTA	388	41,181
	10 20 30 40 50 60 MALQLLPSTL SVPKKGSSMG AVAVKDTAAF LGVSSKAKKA SLAVRTQVAT APSPVTTSPG 70 80 90 100 110 120 STASSPSGKK TLRQGVVVIT GASSGLGLAA AKALAETGKW HVVMACRDFL KASKAAKAAG 130 140 150 160 170 180 MADGSYTVMH LDLASLDSVR QFVDAFRRAE MPLDVLVCNA AIYRPTARTP TFTADGHEMS 190 200 210 220 230 240 VGVNHLGHFL LARLLMEDLQ KSDYPSRRMV IVGSITGNSN TLAGNVPPKA SLGDLRGLAG 250 260 270 280 290 300 GLSGASGSAM IDGDESFDGA KAYKDSKVCN MLTMQEFHRR YHEETGITFS SLYPGCIATT 310 320 330 340 350 360 GLFREHIPLF RTLFPPFQKF VTKGFVSEAE SGKRLAQVVA EPVLTKSGVY WSWNKDSASF 370 380 ENQLSQEASD PEKARKVWEL SEKLVGLA

References

[1]

"Nucleotide sequence of a cDNA coding for the NADPH-protochlorophyllide oxidoreductase (PCR) of barley (Hordeum vulgare L.) and its expression in Escherichia coli." Schuelz R., Steinmueller K., Klaas M., Forreiter C., Rasmussen S., Hiller C., Apel K. Mol. Gen. Genet. 217:355-361(1989) [PubMed: 2671659] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: cv. Carina. Tissue: Leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X15869 mRNA. Translation: CAA33879.1.
PIR	S04783.
3D structure databases
HSSP	HSSP built from PDB template 1FDS based on UniProtKB P14061.
ModBase	Search...
Organism-specific databases
Gramene	P13653.
Family and domain databases
InterPro	IPR002198. DHase_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DHase. IPR016040. NAD(P)-bd. IPR005979. Prochl_reduct. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH.
TIGRFAMs	TIGR01289. LPOR. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PORA_HORVU

Accession

Primary (citable) accession number: P13653

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	November 25, 2008
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)