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P13653

- PORA_HORVU

UniProt

P13653 - PORA_HORVU

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Protein

Protochlorophyllide reductase A, chloroplastic

Gene

PORA

Organism
Hordeum vulgare (Barley)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Phototransformation of protochlorophyllide (Pchlide) to chlorophyllide (Chlide).

Catalytic activityi

Chlorophyllide a + NADP+ = protochlorophyllide + NADPH.

Pathwayi

GO - Molecular functioni

  1. protochlorophyllide reductase activity Source: UniProtKB-EC

GO - Biological processi

  1. chlorophyll biosynthetic process Source: UniProtKB-UniPathway
  2. photosynthesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Chlorophyll biosynthesis, Photosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BRENDAi1.3.1.33. 2687.
UniPathwayiUPA00668.

Names & Taxonomyi

Protein namesi
Recommended name:
Protochlorophyllide reductase A, chloroplastic (EC:1.3.1.33)
Short name:
PCR A
Alternative name(s):
NADPH-protochlorophyllide oxidoreductase A
Short name:
POR A
Gene namesi
Name:PORA
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeHordeum

Organism-specific databases

GrameneiP13653.

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 7474ChloroplastAdd
BLAST
Chaini75 – 388314Protochlorophyllide reductase A, chloroplasticPRO_0000023293Add
BLAST

Expressioni

Developmental stagei

Active only transiently in etiolated seedlings at the beginning of illumination.

Gene expression databases

GenevestigatoriP13653.

Interactioni

Protein-protein interaction databases

DIPiDIP-46278N.

Structurei

3D structure databases

ProteinModelPortaliP13653.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR005979. Prochl_reduct.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
TIGRFAMsiTIGR01289. LPOR. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13653-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALQLLPSTL SVPKKGSSMG AVAVKDTAAF LGVSSKAKKA SLAVRTQVAT
60 70 80 90 100
APSPVTTSPG STASSPSGKK TLRQGVVVIT GASSGLGLAA AKALAETGKW
110 120 130 140 150
HVVMACRDFL KASKAAKAAG MADGSYTVMH LDLASLDSVR QFVDAFRRAE
160 170 180 190 200
MPLDVLVCNA AIYRPTARTP TFTADGHEMS VGVNHLGHFL LARLLMEDLQ
210 220 230 240 250
KSDYPSRRMV IVGSITGNSN TLAGNVPPKA SLGDLRGLAG GLSGASGSAM
260 270 280 290 300
IDGDESFDGA KAYKDSKVCN MLTMQEFHRR YHEETGITFS SLYPGCIATT
310 320 330 340 350
GLFREHIPLF RTLFPPFQKF VTKGFVSEAE SGKRLAQVVA EPVLTKSGVY
360 370 380
WSWNKDSASF ENQLSQEASD PEKARKVWEL SEKLVGLA
Length:388
Mass (Da):41,181
Last modified:January 1, 1990 - v1
Checksum:iEBD3EF153D96C129
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X15869 mRNA. Translation: CAA33879.1.
PIRiS04783.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X15869 mRNA. Translation: CAA33879.1 .
PIRi S04783.

3D structure databases

ProteinModelPortali P13653.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-46278N.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

Gramenei P13653.

Enzyme and pathway databases

UniPathwayi UPA00668 .
BRENDAi 1.3.1.33. 2687.

Gene expression databases

Genevestigatori P13653.

Family and domain databases

Gene3Di 3.40.50.720. 2 hits.
InterProi IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR005979. Prochl_reduct.
[Graphical view ]
Pfami PF00106. adh_short. 1 hit.
[Graphical view ]
PRINTSi PR00081. GDHRDH.
TIGRFAMsi TIGR01289. LPOR. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of a cDNA coding for the NADPH-protochlorophyllide oxidoreductase (PCR) of barley (Hordeum vulgare L.) and its expression in Escherichia coli."
    Schuelz R., Steinmueller K., Klaas M., Forreiter C., Rasmussen S., Hiller C., Apel K.
    Mol. Gen. Genet. 217:355-361(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: cv. Carina.
    Tissue: Leaf.

Entry informationi

Entry nameiPORA_HORVU
AccessioniPrimary (citable) accession number: P13653
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: October 29, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3