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Protein

Protochlorophyllide reductase A, chloroplastic

Gene

PORA

Organism
Hordeum vulgare (Barley)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Phototransformation of protochlorophyllide (Pchlide) to chlorophyllide (Chlide).

Catalytic activityi

Chlorophyllide a + NADP+ = protochlorophyllide + NADPH.

Pathway:ichlorophyll biosynthesis

This protein is involved in the pathway chlorophyll biosynthesis, which is part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the pathway chlorophyll biosynthesis and in Porphyrin-containing compound metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Chlorophyll biosynthesis, Photosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BRENDAi1.3.1.33. 2687.
UniPathwayiUPA00668.

Names & Taxonomyi

Protein namesi
Recommended name:
Protochlorophyllide reductase A, chloroplastic (EC:1.3.1.33)
Short name:
PCR A
Alternative name(s):
NADPH-protochlorophyllide oxidoreductase A
Short name:
POR A
Gene namesi
Name:PORA
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaeTriticodaeTriticeaeHordeinaeHordeum

Organism-specific databases

GrameneiP13653.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 7474ChloroplastAdd
BLAST
Chaini75 – 388314Protochlorophyllide reductase A, chloroplasticPRO_0000023293Add
BLAST

Expressioni

Developmental stagei

Active only transiently in etiolated seedlings at the beginning of illumination.

Interactioni

Protein-protein interaction databases

DIPiDIP-46278N.
STRINGi4513.MLOC_1579.2.

Structurei

3D structure databases

ProteinModelPortaliP13653.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR005979. Prochl_reduct.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
TIGRFAMsiTIGR01289. LPOR. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13653-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALQLLPSTL SVPKKGSSMG AVAVKDTAAF LGVSSKAKKA SLAVRTQVAT
60 70 80 90 100
APSPVTTSPG STASSPSGKK TLRQGVVVIT GASSGLGLAA AKALAETGKW
110 120 130 140 150
HVVMACRDFL KASKAAKAAG MADGSYTVMH LDLASLDSVR QFVDAFRRAE
160 170 180 190 200
MPLDVLVCNA AIYRPTARTP TFTADGHEMS VGVNHLGHFL LARLLMEDLQ
210 220 230 240 250
KSDYPSRRMV IVGSITGNSN TLAGNVPPKA SLGDLRGLAG GLSGASGSAM
260 270 280 290 300
IDGDESFDGA KAYKDSKVCN MLTMQEFHRR YHEETGITFS SLYPGCIATT
310 320 330 340 350
GLFREHIPLF RTLFPPFQKF VTKGFVSEAE SGKRLAQVVA EPVLTKSGVY
360 370 380
WSWNKDSASF ENQLSQEASD PEKARKVWEL SEKLVGLA
Length:388
Mass (Da):41,181
Last modified:January 1, 1990 - v1
Checksum:iEBD3EF153D96C129
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15869 mRNA. Translation: CAA33879.1.
PIRiS04783.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15869 mRNA. Translation: CAA33879.1.
PIRiS04783.

3D structure databases

ProteinModelPortaliP13653.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-46278N.
STRINGi4513.MLOC_1579.2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

GrameneiP13653.

Enzyme and pathway databases

UniPathwayiUPA00668.
BRENDAi1.3.1.33. 2687.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR005979. Prochl_reduct.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
TIGRFAMsiTIGR01289. LPOR. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence of a cDNA coding for the NADPH-protochlorophyllide oxidoreductase (PCR) of barley (Hordeum vulgare L.) and its expression in Escherichia coli."
    Schuelz R., Steinmueller K., Klaas M., Forreiter C., Rasmussen S., Hiller C., Apel K.
    Mol. Gen. Genet. 217:355-361(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: cv. Carina.
    Tissue: Leaf.

Entry informationi

Entry nameiPORA_HORVU
AccessioniPrimary (citable) accession number: P13653
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: July 22, 2015
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.