P13640 (MT1G_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Metallothionein-1G
Short name=MT-1G

Alternative name(s):
Metallothionein-1K
Short name=MT-1K
Metallothionein-IG
Short name=MT-IG

Gene names

Name:	MT1G
Synonyms:	MT1K, MT1M

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	62 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.
Subunit structure	Monomer.
Domain	Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.
Sequence similarities	Belongs to the metallothionein superfamily. Type 1 family.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	Cadmium Copper Metal-binding Metal-thiolate cluster Zinc
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to cadmium ion Inferred from expression pattern PubMed 16827180PubMed 19330122Ref.1. Source: UniProtKB cellular response to copper ion Inferred from expression pattern Ref.1. Source: UniProtKB cellular response to vascular endothelial growth factor stimulus Inferred from expression pattern PubMed 15735762. Source: UniProtKB cellular response to zinc ion Inferred from direct assay PubMed 7559655. Source: UniProtKB monocyte activation Non-traceable author statement Ref.5. Source: UniProtKB monocyte differentiation Non-traceable author statement Ref.5. Source: UniProtKB negative regulation of growth Inferred from direct assay PubMed 18332874. Source: UniProtKB
Cellular_component	nucleus Inferred from direct assay PubMed 15735762PubMed 7559655. Source: UniProtKB perinuclear region of cytoplasm Inferred from direct assay PubMed 15735762. Source: UniProtKB
Molecular_function	zinc ion binding Inferred from direct assay Ref.5. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SPINK7	P58062	3	EBI-1182473,EBI-1182445

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P13640-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P13640-2)

The sequence of this isoform differs from the canonical sequence as follows:
     10-10: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 62	62	Metallothionein-1G		PRO_0000197238
Regions
	Region	1 – 29	29	Beta
	Region	31 – 62	32	Alpha
Sites
	Metal binding	5	1	Divalent metal cation; cluster B
	Metal binding	7	1	Divalent metal cation; cluster B
	Metal binding	14	1	Divalent metal cation; cluster B
	Metal binding	16	1	Divalent metal cation; cluster B
	Metal binding	20	1	Divalent metal cation; cluster B
	Metal binding	22	1	Divalent metal cation; cluster B
	Metal binding	25	1	Divalent metal cation; cluster B
	Metal binding	27	1	Divalent metal cation; cluster B
	Metal binding	30	1	Divalent metal cation; cluster B
	Metal binding	34	1	Divalent metal cation; cluster A
	Metal binding	35	1	Divalent metal cation; cluster A
	Metal binding	37	1	Divalent metal cation; cluster A
	Metal binding	38	1	Divalent metal cation; cluster A
	Metal binding	42	1	Divalent metal cation; cluster A
	Metal binding	45	1	Divalent metal cation; cluster A
	Metal binding	49	1	Divalent metal cation; cluster A
	Metal binding	51	1	Divalent metal cation; cluster A
	Metal binding	58	1	Divalent metal cation; cluster A
	Metal binding	60	1	Divalent metal cation; cluster A
	Metal binding	61	1	Divalent metal cation; cluster A
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine
Natural variations
	Alternative sequence	10	1	Missing in isoform 2.		VSP_017230

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 7, 2006. Version 2. Checksum: A1B390F5899DD040	FASTA	62	6,141
	10 20 30 40 50 60 MDPNCSCAAA GVSCTCASSC KCKECKCTSC KKSCCSCCPV GCAKCAQGCI CKGASEKCSC CA
	Isoform 2 [UniParc]. Checksum: 7B0A61FF89CA94CC	FASTA	61	6,070
	10 20 30 40 50 60 MDPNCSCAAG VSCTCASSCK CKECKCTSCK KSCCSCCPVG CAKCAQGCIC KGASEKCSCC A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and expression of the human metallothionein-IG gene. Differential promoter activity of two linked metallothionein-I genes in response to heavy metals." Foster R., Jahroudi N., Varshney U., Gedamu L. J. Biol. Chem. 263:11528-11535(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Structure and expression of the human metallothionein genes." Gedamu L., Varshney U., Jahroudi N., Foster R., Shworak N.W. Experientia Suppl. 52:361-372(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	Hunziker P.E. Submitted (NOV-1993) to UniProtKB Cited for: PROTEIN SEQUENCE (ISOFORM 1). Tissue: Liver.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Liver.
[5]	"Induction by zinc of specific metallothionein isoforms in human monocytes." Pauwels M., van Weyenbergh J., Soumillion A., Proost P., Ley M. Eur. J. Biochem. 220:105-110(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-31 (ISOFORM 2).

Cross-references

Sequence databases
EMBL GenBank DDBJ	J03910 Genomic DNA. Translation: AAA59873.1. BC020757 mRNA. Translation: AAH20757.1. S68954 mRNA. Translation: AAB30083.1.
IPI	IPI00008752. IPI00413064.
PIR	SMHU1G. A29236.
RefSeq	NP_005941.1. NM_005950.1.
UniGene	Hs.433391.
3D structure databases
ProteinModelPortal	P13640.
ModBase	Search...
Protein-protein interaction databases
IntAct	P13640. 2 interactions.
STRING	9606.ENSP00000391397.
PTM databases
PhosphoSite	P13640.
Polymorphism databases
DMDM	90109444.
Proteomic databases
PaxDb	P13640.
PRIDE	P13640.
Protocols and materials databases
DNASU	4495.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000379811; ENSP00000369139; ENSG00000125144. ENST00000444837; ENSP00000391397; ENSG00000125144.
GeneID	4495.
KEGG	hsa:4495.
UCSC	uc002eju.1. human.
Organism-specific databases
CTD	4495.
GeneCards	GC16M056700.
H-InvDB	HIX0021664.
HGNC	HGNC:7399. MT1G.
MIM	156353. gene.
neXtProt	NX_P13640.
PharmGKB	PA31204.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG138665.
HOGENOM	HOG000236262.
InParanoid	P13640.
KO	K14739.
OMA	CKEASAK.
OrthoDB	EOG48KRD3.
Gene expression databases
ArrayExpress	P13640.
Bgee	P13640.
CleanEx	HS_MT1G. HS_MT1M.
Genevestigator	P13640.
GermOnline	ENSG00000125144. Homo sapiens.
Family and domain databases
Gene3D	4.10.10.10. 1 hit.
InterPro	IPR017854. Metalthion_dom. IPR023587. Metalthion_dom_vert. IPR003019. Metalthion_sfam_euk. IPR000006. Metalthion_vert. IPR018064. Metalthion_vert_metal_BS. [Graphical view]
PANTHER	PTHR23299. PTHR23299. 1 hit.
Pfam	PF00131. Metallothio. 1 hit. [Graphical view]
PRINTS	PR00860. MTVERTEBRATE.
SUPFAM	SSF57868. Metallothionein_sfam. 1 hit.
PROSITE	PS00203. METALLOTHIONEIN_VRT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	MT1G. human.
GenomeRNAi	4495.
NextBio	17383.
SOURCE	Search...

Entry information

Entry name

MT1G_HUMAN

Accession

Primary (citable) accession number: P13640
Secondary accession number(s): P80296

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	February 7, 2006
Last modified:	May 1, 2013
This is version 114 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.