Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Metallothionein-1G

Gene

MT1G

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi5 – 51Divalent metal cation; cluster B
Metal bindingi7 – 71Divalent metal cation; cluster B
Metal bindingi14 – 141Divalent metal cation; cluster B
Metal bindingi16 – 161Divalent metal cation; cluster B
Metal bindingi20 – 201Divalent metal cation; cluster B
Metal bindingi22 – 221Divalent metal cation; cluster B
Metal bindingi25 – 251Divalent metal cation; cluster B
Metal bindingi27 – 271Divalent metal cation; cluster B
Metal bindingi30 – 301Divalent metal cation; cluster B
Metal bindingi34 – 341Divalent metal cation; cluster A
Metal bindingi35 – 351Divalent metal cation; cluster A
Metal bindingi37 – 371Divalent metal cation; cluster A
Metal bindingi38 – 381Divalent metal cation; cluster A
Metal bindingi42 – 421Divalent metal cation; cluster A
Metal bindingi45 – 451Divalent metal cation; cluster A
Metal bindingi49 – 491Divalent metal cation; cluster A
Metal bindingi51 – 511Divalent metal cation; cluster A
Metal bindingi58 – 581Divalent metal cation; cluster A
Metal bindingi60 – 601Divalent metal cation; cluster A
Metal bindingi61 – 611Divalent metal cation; cluster A

GO - Molecular functioni

  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • cellular response to cadmium ion Source: UniProtKB
  • cellular response to copper ion Source: UniProtKB
  • cellular response to vascular endothelial growth factor stimulus Source: UniProtKB
  • cellular response to zinc ion Source: UniProtKB
  • monocyte activation Source: UniProtKB
  • monocyte differentiation Source: UniProtKB
  • negative regulation of growth Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

Cadmium, Copper, Metal-binding, Metal-thiolate cluster, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Metallothionein-1G
Short name:
MT-1G
Alternative name(s):
Metallothionein-1K
Short name:
MT-1K
Metallothionein-IG
Short name:
MT-IG
Gene namesi
Name:MT1G
Synonyms:MT1K, MT1M
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:7399. MT1G.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • nucleus Source: UniProtKB
  • perinuclear region of cytoplasm Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA31204.

Polymorphism and mutation databases

DMDMi90109444.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6262Metallothionein-1GPRO_0000197238Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication
Modified residuei59 – 591PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP13640.
PaxDbiP13640.
PRIDEiP13640.

PTM databases

PhosphoSiteiP13640.

Expressioni

Gene expression databases

BgeeiP13640.
CleanExiHS_MT1G.
HS_MT1M.
ExpressionAtlasiP13640. baseline and differential.
GenevisibleiP13640. HS.

Interactioni

Subunit structurei

Monomer.

Binary interactionsi

WithEntry#Exp.IntActNotes
SPINK7P580623EBI-1182473,EBI-1182445

Protein-protein interaction databases

BioGridi110601. 2 interactions.
IntActiP13640. 2 interactions.
STRINGi9606.ENSP00000391397.

Structurei

3D structure databases

ProteinModelPortaliP13640.
SMRiP13640. Positions 1-62.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 2929BetaAdd
BLAST
Regioni31 – 6232AlphaAdd
BLAST

Domaini

Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG138665.
GeneTreeiENSGT00730000110883.
HOGENOMiHOG000236262.
InParanoidiP13640.
KOiK14739.
OMAiCCKECIC.
OrthoDBiEOG7PZS1X.
PhylomeDBiP13640.
TreeFamiTF336054.

Family and domain databases

Gene3Di4.10.10.10. 1 hit.
InterProiIPR017854. Metalthion_dom.
IPR023587. Metalthion_dom_vert.
IPR003019. Metalthion_sfam_euk.
IPR000006. Metalthion_vert.
IPR018064. Metalthion_vert_metal_BS.
[Graphical view]
PANTHERiPTHR23299. PTHR23299. 1 hit.
PfamiPF00131. Metallothio. 1 hit.
[Graphical view]
PRINTSiPR00860. MTVERTEBRATE.
SUPFAMiSSF57868. SSF57868. 1 hit.
PROSITEiPS00203. METALLOTHIONEIN_VRT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P13640-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDPNCSCAAA GVSCTCASSC KCKECKCTSC KKSCCSCCPV GCAKCAQGCI
60
CKGASEKCSC CA
Length:62
Mass (Da):6,141
Last modified:February 7, 2006 - v2
Checksum:iA1B390F5899DD040
GO
Isoform 2 (identifier: P13640-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     10-10: Missing.

Show »
Length:61
Mass (Da):6,070
Checksum:i7B0A61FF89CA94CC
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei10 – 101Missing in isoform 2. 1 PublicationVSP_017230

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03910 Genomic DNA. Translation: AAA59873.1.
BC020757 mRNA. Translation: AAH20757.1.
S68954 mRNA. Translation: AAB30083.1.
CCDSiCCDS10766.1. [P13640-2]
CCDS76873.1. [P13640-1]
PIRiA29236. SMHU1G.
RefSeqiNP_001288196.1. NM_001301267.1. [P13640-1]
NP_005941.1. NM_005950.2. [P13640-2]
UniGeneiHs.433391.

Genome annotation databases

EnsembliENST00000379811; ENSP00000369139; ENSG00000125144.
ENST00000444837; ENSP00000391397; ENSG00000125144. [P13640-2]
GeneIDi4495.
KEGGihsa:4495.
UCSCiuc002eju.1. human. [P13640-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03910 Genomic DNA. Translation: AAA59873.1.
BC020757 mRNA. Translation: AAH20757.1.
S68954 mRNA. Translation: AAB30083.1.
CCDSiCCDS10766.1. [P13640-2]
CCDS76873.1. [P13640-1]
PIRiA29236. SMHU1G.
RefSeqiNP_001288196.1. NM_001301267.1. [P13640-1]
NP_005941.1. NM_005950.2. [P13640-2]
UniGeneiHs.433391.

3D structure databases

ProteinModelPortaliP13640.
SMRiP13640. Positions 1-62.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110601. 2 interactions.
IntActiP13640. 2 interactions.
STRINGi9606.ENSP00000391397.

PTM databases

PhosphoSiteiP13640.

Polymorphism and mutation databases

DMDMi90109444.

Proteomic databases

MaxQBiP13640.
PaxDbiP13640.
PRIDEiP13640.

Protocols and materials databases

DNASUi4495.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000379811; ENSP00000369139; ENSG00000125144.
ENST00000444837; ENSP00000391397; ENSG00000125144. [P13640-2]
GeneIDi4495.
KEGGihsa:4495.
UCSCiuc002eju.1. human. [P13640-2]

Organism-specific databases

CTDi4495.
GeneCardsiGC16M056700.
H-InvDBHIX0021664.
HGNCiHGNC:7399. MT1G.
MIMi156353. gene.
neXtProtiNX_P13640.
PharmGKBiPA31204.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG138665.
GeneTreeiENSGT00730000110883.
HOGENOMiHOG000236262.
InParanoidiP13640.
KOiK14739.
OMAiCCKECIC.
OrthoDBiEOG7PZS1X.
PhylomeDBiP13640.
TreeFamiTF336054.

Miscellaneous databases

ChiTaRSiMT1G. human.
GeneWikiiMetallothionein_1G.
GenomeRNAii4495.
NextBioi17383.
PROiP13640.
SOURCEiSearch...

Gene expression databases

BgeeiP13640.
CleanExiHS_MT1G.
HS_MT1M.
ExpressionAtlasiP13640. baseline and differential.
GenevisibleiP13640. HS.

Family and domain databases

Gene3Di4.10.10.10. 1 hit.
InterProiIPR017854. Metalthion_dom.
IPR023587. Metalthion_dom_vert.
IPR003019. Metalthion_sfam_euk.
IPR000006. Metalthion_vert.
IPR018064. Metalthion_vert_metal_BS.
[Graphical view]
PANTHERiPTHR23299. PTHR23299. 1 hit.
PfamiPF00131. Metallothio. 1 hit.
[Graphical view]
PRINTSiPR00860. MTVERTEBRATE.
SUPFAMiSSF57868. SSF57868. 1 hit.
PROSITEiPS00203. METALLOTHIONEIN_VRT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and expression of the human metallothionein-IG gene. Differential promoter activity of two linked metallothionein-I genes in response to heavy metals."
    Foster R., Jahroudi N., Varshney U., Gedamu L.
    J. Biol. Chem. 263:11528-11535(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Structure and expression of the human metallothionein genes."
    Gedamu L., Varshney U., Jahroudi N., Foster R., Shworak N.W.
    Experientia Suppl. 52:361-372(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Hunziker P.E.
    Submitted (NOV-1993) to UniProtKB
    Cited for: PROTEIN SEQUENCE (ISOFORM 1).
    Tissue: Liver.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Liver.
  5. "Induction by zinc of specific metallothionein isoforms in human monocytes."
    Pauwels M., van Weyenbergh J., Soumillion A., Proost P., Ley M.
    Eur. J. Biochem. 220:105-110(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-31 (ISOFORM 2), ACETYLATION AT MET-1.

Entry informationi

Entry nameiMT1G_HUMAN
AccessioniPrimary (citable) accession number: P13640
Secondary accession number(s): P80296
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: February 7, 2006
Last modified: July 22, 2015
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Metallothioneins
    Classification of metallothioneins and list of entries
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.