Reviewed, UniProtKB/Swiss-Prot P13636 (PEPA_URSTH)

Names and origin

Protein names

Recommended name:
    Pepsin A
    EC=3.4.23.1

Gene names

Name:

PGA

Organism

Ursus thibetanus (Asiatic black bear)

Taxonomic identifier

9642 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Ursidae › Ursus

Protein attributes

Sequence length	60 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.
Catalytic activity	Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves 1-Phe-|-Val-2, 4-Gln-|-His-5, 13-Glu-|-Ala-14, 14-Ala-|-Leu-15, 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17, 23-Gly-|-Phe-24, 24-Phe-|-Phe-25 and 25-Phe-|-Tyr-26 bonds in the B chain of insulin.
Subcellular location	Secreted.
Sequence similarities	Belongs to the peptidase A1 family.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Molecular function	Aspartyl protease Hydrolase Protease
PTM	Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 45	45	Activation peptide		PRO_0000026044
	Chain	46 – ›60	›15	Pepsin A		PRO_0000026045
Experimental info
	Non-terminal residue	60	1

Sequences

	Sequence		Length	Mass (Da)
	P13636-1 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: C6BFDC560D4EEE1A	FASTA	60	7,052
	10 20 30 40 50 60 FIIKVPLVKK KSLRKNLKEH GLLKDFLKKH SPNPASKYFP QEAAVMATQP LENYMDMEYF

References

[1]

"Purification and characterization of pepsinogens and pepsins from Asiatic black bear, and amino acid sequence determination of the NH2-terminal 60 residues of the major pepsinogen." Kageyama T., Moriyama A., Takahashi K. J. Biochem. 94:1557-1567(1983) [PubMed: 6418734] [Abstract] Cited for: PROTEIN SEQUENCE.

Cross-references

Sequence databases
PIR	A28859.
3D structure databases
HSSP	HSSP built from PDB template 2PSG based on UniProtKB P00791.
ModBase	Search...
Protein family/group databases
MEROPS	A01.001.
Phylogenomic databases
HOVERGEN	P13636.
Enzyme and pathway databases
BRENDA	3.4.23.1. 296602.
Family and domain databases
InterPro	IPR001461. Peptidase_A1. IPR001969. Peptidase_aspartic_AS. IPR012848. Propep_A1. [Graphical view]
PANTHER	PTHR13683. Peptidase_A1. 1 hit.
Pfam	PF07966. A1_Propeptide. 1 hit. [Graphical view]
PROSITE	PS00141. ASP_PROTEASE. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PEPA_URSTH

Accession

Primary (citable) accession number: P13636

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	June 16, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)