Reviewed, UniProtKB/Swiss-Prot P13633 (DCTB_RHIME)

Names and origin

Protein names

Recommended name:
    C4-dicarboxylate transport sensor protein dctB
    EC=2.7.13.3

Gene names

Name:	dctB
Ordered Locus Names:	RB1524
ORF Names:	SMb20612

Encoded on

Plasmid pSymB (megaplasmid 2)

Organism

Rhizobium meliloti (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

382 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	621 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. DctB functions as a membrane-associated protein kinase that phosphorylates dctD in response to environmental signals.
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Contains 1 histidine kinase domain.
Sequence caution	The sequence M33555 differs from that shown. Reason: Frameshift at positions 20 and 161.

Ontologies

Keywords
Biological process	Two-component regulatory system
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Plasmid
Gene Ontology (GO)
Biological process	peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 621

621

C4-dicarboxylate transport sensor protein dctB

PRO_0000074748

Regions

Topological domain

1 – 26

26

Cytoplasmic Potential

Transmembrane

27 – 45

19

Potential

Topological domain

46 – 320

275

Periplasmic

Transmembrane

321 – 338

18

Potential

Topological domain

339 – 621

283

Cytoplasmic Potential

Domain

412 – 621

210

Histidine kinase

Amino acid modifications

Modified residue

415

1

Phosphohistidine; by autocatalysis By similarity

Experimental info

Sequence conflict

3

1

H → N Ref.1

Sequence conflict

3

1

H → N Ref.5

Sequence conflict

102

1

P → Q in AAA63511. Ref.1

Sequence conflict

102

1

P → Q in AAA63510. Ref.1

Sequence conflict

174

1

N → K Ref.5

Sequence conflict

504

1

L → I in AAA63511. Ref.1

Sequence conflict

504

1

L → I in AAA63510. Ref.1

Sequence conflict

540

1

D → N in AAA63511. Ref.1

Sequence conflict

540

1

D → N in AAA63510. Ref.1

Sequence conflict

548 – 551

4

EEAA → KAAP in AAA63511. Ref.1

Sequence conflict

548 – 551

4

EEAA → KAAP in AAA63510. Ref.1

Sequence conflict

615

1

A → T in AAA63511. Ref.1

Sequence conflict

615

1

A → T in AAA63510. Ref.1

Secondary structure

1

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621

Helix Strand Turn

Details...

Helix

61 – 79

19

Helix

81 – 87

7

Turn

90 – 92

3

Helix

101 – 117

17

Beta strand

121 – 126

6

Beta strand

132 – 135

4

Turn

136 – 139

4

Helix

153 – 160

8

Beta strand

164 – 166

3

Beta strand

179 – 182

4

Beta strand

194 – 197

4

Helix

204 – 209

6

Beta strand

214 – 217

4

Beta strand

223 – 228

6

Helix

229 – 231

3

Beta strand

235 – 237

3

Helix

241 – 249

9

Beta strand

264 – 268

5

Beta strand

274 – 278

5

Beta strand

286 – 294

9

Beta strand

301 – 307

7

Sequences

	Sequence		Length	Mass (Da)
	P13633-1 [UniParc]. Last modified November 2, 2001. Version 3. Checksum: D60F724E2CA6BCC7	FASTA	621	67,133
	10 20 30 40 50 60 MHHVRMVKLP AEASDPHALR SRARRSWLVF AAVALVLLAA GLLLARDYGR SQALAGLAGQ 70 80 90 100 110 120 SRIDASLKAS LLRAVVERQR ALPLVLADDA AIRGALLSPD RPSLDRINRK LEALATSAEA 130 140 150 160 170 180 AVIYLIDRSG VAVAASNWQE PTSFVGNDYA FRDYFRLAVR DGMAEHFAMG TVSNRPGLYI 190 200 210 220 230 240 SRRVDGPGGP LGVIVAKLEF DGVEADWQAS GKPAYVTDRR GIVLITSLPS WRFMTTKPIA 250 260 270 280 290 300 EDRLAPIRES LQFGDAPLLP LPFRKIEARP DGSSTLDALL PGDSTAAFLR VETMVPSTNW 310 320 330 340 350 360 RLEQLSPLKA PLAAGAREAQ LLTLAALVPL LALAALLLRR RQVVAMRSAE ERLARNALEA 370 380 390 400 410 420 SVEERTRDLR MARDRLETEI ADHRQTTEKL QAVQQDLVQA NRLAILGQVA AGVAHEINQP 430 440 450 460 470 480 VATIRAYADN ARTFLHRGQT VTAAENMESI AELTERVGAI TDELRRFARK GHFAAGPTAM 490 500 510 520 530 540 KEVVEGALML LRSRFAGRMD AIRLDLPPDG LQALGNRIRL EQVLINLLQN ALEAIGDSED 550 560 570 580 590 600 GAIQVRCEEA AGGIALTVAD NGPGIAADVR EELFTPFNTS KEDGLGLGLA ISKEIVSDYG 610 620 GTIEVESGPS GTTFAVNLKK A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the C4-dicarboxylate transport genes of Rhizobium meliloti: nucleotide sequence and deduced products of dctA, dctB, and dctD." Watson R.J. Mol. Plant Microbe Interact. 3:174-181(1990) [PubMed: 2134335] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: JJ1c10.
[2]	"Conservation between coding and regulatory elements of Rhizobium meliloti and Rhizobium leguminosarum dct genes." Jiang J., Gu B., Albright L.M., Nixon B.T. J. Bacteriol. 171:5244-5253(1989) [PubMed: 2793824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 1021.
[3]	"The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing endosymbiont Sinorhizobium meliloti." Finan T.M., Weidner S., Wong K., Buhrmester J., Chain P., Vorhoelter F.J., Hernandez-Lucas I., Becker A., Cowie A., Gouzy J., Golding B., Puehler A. Proc. Natl. Acad. Sci. U.S.A. 98:9889-9894(2001) [PubMed: 11481431] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.
[4]	"Identification and sequence analysis of the Rhizobium meliloti dctA gene encoding the C4-dicarboxylate carrier." Engelke T., Jording D., Kapp D., Puehler A. J. Bacteriol. 171:5551-5560(1989) [PubMed: 2551890] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-185. Strain: RCR2011 / SU47.
[5]	"Genetic analysis and regulation of the Rhizobium meliloti genes controlling C4-dicarboxylic acid transport." Wang Y.-P., Birkenhead K., Boesten B., Manian S., O'Gara F. Gene 85:135-144(1989) [PubMed: 2695394] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-177.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

J03683 Genomic DNA. Translation: AAA63511.1.
J03683 Genomic DNA. Translation: AAA63510.1. Different initiation.
J03683 Genomic DNA. Translation: AAA63512.1. Different initiation.
M26531 Genomic DNA. Translation: AAA26249.1. Different initiation.
AL591985 Genomic DNA. Translation: CAC49924.1.
M26399 Genomic DNA. Translation: AAA26251.2.
M33555 Genomic DNA. No translation available.

PIR

B33586.
D96032.

RefSeq

NP_438064.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2ZBB	X-ray	2.50	A/B/C/D	42-312	[»]
3E4O	X-ray	2.30	A/B	42-312	[»]
3E4P	X-ray	2.30	A/B	42-312	[»]
3E4Q	X-ray	2.75	A/B	42-312	[»]

ModBase

Search...

Genome annotation databases

GeneID

1237856.

GenomeReviews

Gene locus RB1524 in contig AL591985_GR.

KEGG

sme:SM_b20612.

NMPDR

fig|266834.1.peg.6159.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

P13633.

OMA

AYADNAR.

Enzyme and pathway databases

BioCyc

SMEL266834:SMB20612-MON.

BRENDA

2.7.13.3. 142.

Family and domain databases

InterPro

IPR003594. ATP_bd_ATPase.
IPR004358. Sig_transdc_His_kin-like_C.
IPR003661. Sig_transdc_His_kin_sub1_dim/P.
IPR005467. Sig_transdc_His_kinase_core.
IPR017055. Sig_transdc_His_kinase_DctB.
[Graphical view]

Gene3D

G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.

Pfam

PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
[Graphical view]

PIRSF

PIRSF036431. STHK_DctB. 1 hit.

PRINTS

PR00344. BCTRLSENSOR.

SMART

SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
[Graphical view]

PROSITE

PS50109. HIS_KIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

DCTB_RHIME

Accession

Primary (citable) accession number: P13633

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	November 2, 2001
Last modified:	November 3, 2009
This is version 98 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)