Reviewed, UniProtKB/Swiss-Prot P13628 (PHFS_DESVO)

Names and origin

Protein names

Recommended name:
    Periplasmic [Fe] hydrogenase small subunit
    EC=1.12.7.2
Alternative name(s):
    Fe hydrogenlyase small chain

Gene names

Name:

hydB

Organism

Desulfovibrio vulgaris (strain Oxamicus Monticello)

Taxonomic identifier

884 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfovibrionales › Desulfovibrionaceae › Desulfovibrio

Protein attributes

Sequence length	124 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Predicted.

General annotation (Comments)

Function	May be involved in hydrogen uptake for the reduction of sulfate to hydrogen sulfide in an electron transport chain. Cytochrome c3 is likely to be the physiological electron carrier for the enzyme.
Catalytic activity	H2 + 2 oxidized ferredoxin = 2 reduced ferredoxin + 2 H+.
Subunit structure	Heterodimer of a large and a small subunit.
Subcellular location	Periplasm.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Ligand	Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro ferredoxin hydrogenase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW iron-sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 34	34	Tat-type signal Potential
	Chain	35 – 124	90	Periplasmic [Fe] hydrogenase small subunit		PRO_0000013414

Sequences

	Sequence		Length	Mass (Da)
	P13628-1 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: 296AF34FD32B21B5	FASTA	124	13,962
	10 20 30 40 50 60 MQIVNLTRRG FLKAACVVTG GALISIRMTG KAVAAAKQLK DYMMDRINGV YGADAKFPVR 70 80 90 100 110 120 ASQDNVQVQK LYADFLEKPM SHKAEQLLHT HWVDRSKAIE RMKAQGAYPN PRAKEFEGNT YPYE

References

[1]	"Organization of the genes encoding [Fe] hydrogenase in Desulfovibrio vulgaris subsp. oxamicus Monticello." Voordouw G., Strang J.D., Wilson F.R. J. Bacteriol. 171:3881-3889(1989) [PubMed: 2661538] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M27212 Genomic DNA. Translation: AAA23374.1.
PIR	HQDVSV. B32886.
3D structure databases
HSSP	HSSP built from PDB template 1HFE based on UniProtKB P07603.
SMR	P13628. Positions 37-124.
ModBase	Search...
Family and domain databases
InterPro	IPR003149. Fe_hydrogenase_ssu-like. IPR006311. Tat. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Gene3D	G3DSA:4.10.260.20. Fe_hyd_ssu-like. 1 hit.
Pfam	PF02256. Fe_hyd_SSU. 1 hit. [Graphical view]
TIGRFAMs	TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PHFS_DESVO

Accession

Primary (citable) accession number: P13628

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)