Reviewed, UniProtKB/Swiss-Prot P13615 (L_VSNJH)

Names and origin

Protein names

Recommended name:
    Large structural protein
      Short name=Protein L
Alternative name(s):
    Transcriptase
    Replicase
Including the following 3 domains:
    1- Recommended name:
            RNA-directed RNA polymerase
              EC=2.7.7.48
    2- Recommended name:
            mRNA (guanine-N(7)-)-methyltransferase
              EC=2.1.1.56
    3- Recommended name:
            mRNA guanylyltransferase
              EC=2.7.7.-

Gene names

Name:

L

Organism

Vesicular stomatitis New Jersey virus (strain Hazelhurst subtype Hazelhurst) (VSNJV)

Taxonomic identifier

11281 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Mononegavirales › Rhabdoviridae › Dimarhabdovirus supergroup › Vesiculovirus

Virus host

Aedes [TaxID: 7158]
Simuliidae (black flies) [TaxID: 7190]
Musca domestica (House fly) [TaxID: 7370]
Homo sapiens (Human) [TaxID: 9606]
Equus asinus (Donkey) [TaxID: 9793]
Equus caballus (Horse) [TaxID: 9796]
Sus scrofa (Pig) [TaxID: 9823]
Bos taurus (Bovine) [TaxID: 9913]
Culicoides [TaxID: 58271]
Lutzomyia [TaxID: 252607]

Protein attributes

Sequence length	2109 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently five subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
Subunit structure	Interacts with the P protein By similarity.
Subcellular location	Virion Potential. Host cytoplasm By similarity.
Sequence similarities	Belongs to the rhabdoviridae protein L family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication mRNA capping mRNA processing
Cellular component	Cytoplasm Virion
Ligand	ATP-binding Nucleotide-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW transcription, RNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2109	2109	Large structural protein		PRO_0000222845
Regions
	Domain	598 – 784	187	RdRp catalytic
	Nucleotide binding	1667 – 1676	10	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P13615-1 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: 52FF2182953E9F79	FASTA	2,109	241,583
	10 20 30 40 50 60 MDFNLIEDST HWEEEESDFL LRDILSKEDQ MSYLNSADYN LNSPLISDDM VYLIKRMNHE 70 80 90 100 110 120 EVPPIWRSKE WDSPLDMLKG CQAQPLSHQD MHNWFGTWIQ NIQHDSAQGF TFLKEVDKEA 130 140 150 160 170 180 EMTYDLVSTF LKGWVGKEYP FKPKGREIDS IALVGPLCQK FLDLHKVTLI LNAVSLGETK 190 200 210 220 230 240 ELLTTFKGKY RMSCENIPIA RLRLPSLGPV FMCKGWTYIH KERVLMDRNF LLMCKDVIIG 250 260 270 280 290 300 RMQTFLSMIG RSDNKFSPDQ IYTLANVYRI GDRILEQCGN RAYDLIKMIE PICNLKMMEL 310 320 330 340 350 360 ARLHRPKIPK FPHFEEHLKG SVRELTKKSN KIQALYDLIM SIKDVDLVLV VYGSFRHWGH 370 380 390 400 410 420 PFIDYFEGLK KLYTQVNMEK NIDKEHPQQL ASDLARLVLL KQFSESKKWF VDLSKMPPKH 430 440 450 460 470 480 PFYEHVVNKT WPTAAKIQDF GDNWHKLPLT QCFEIPDLID PSVIYSDKSH SMNKKEVIQH 490 500 510 520 530 540 VRTKPNIPIP SKKELQTMLT NKATNWKVFL KDIDGNGLDD DDLIIGLKGK ERELKIAGRF 550 560 570 580 590 600 FSLMSWRLRE YFVITEYLIK TYYVPLFKGL TMADDLTSVI KKMMDSSSGQ GLDDYSSVCL 610 620 630 640 650 660 ANHIDYEQWN NHQRKESNGP IFRVMGQFLG YPSLIERIHE FFEKSLIYYN GLPDLLTIRN 670 680 690 700 710 720 GTLCNSTKHR VCWNGQKGGL EGLRQKGWSI VNLLVIQREA KIRNTAVKVL AQGDNQVICT 730 740 750 760 770 780 QYKTRKTRSE LELRAVLHQM AGNNNKIMEE IKRGTEKLGL IINDDETMQS ADYLNYGKIP 790 800 810 820 830 840 IFRGVIRGLE TKRWSRVTCV TNDQIPTCAN LMSSVSTNAL TVAHFAESPI NAMIQYNYFG 850 860 870 880 890 900 TFARLLLFMH DPAIRQSLYT VKEKIPGLHT RTFKYAVLYL DPSIGGECGM ALSRFLIRAF 910 920 930 940 950 960 PDPETESLSF WKFIYEHARS LHLKKMAVMF GDLPIAKFRI EHINKLLEDP TSLNISMGMS 970 980 990 1000 1010 1020 PANLLKSEVK KCLIESRSSI KNEIIKDATI YMHQEEEKLR GFLWSINPLF PRFLSEFKAG 1030 1040 1050 1060 1070 1080 TFLGVSEALI NLFQNSRTIR NSFKRRYHKD LDELIIKSEI SSLSHLGSMH YRLGDNHIWS 1090 1100 1110 1120 1130 1140 CSASRADVLR YKSWTRKVVG TTVPHPLEMH GSPSKKENPC QLCNSSGLTY ISVHCPKGIT 1150 1160 1170 1180 1190 1200 DVFNRRGPLP AYLGSNTSES TSILQPWEKE SKIPIIKRAT RLRDAISWFI PPESPLSTCI 1210 1220 1230 1240 1250 1260 LNNIRALTGE DWSSKQHGFK RTGSALHRFS TSRMSNGGFA SQSPATLTRM IATTDTMRDF 1270 1280 1290 1300 1310 1320 GTKNYDFMFQ ASLLYGQMTT SISRYGSPGS CTDHYHIRCK GCIREIEEVE LNTSLEYRTP 1330 1340 1350 1360 1370 1380 VVYHILEKWR NNTGSWGHQI KQLKPAEGNW ESLSPVEQSY QVARCIGFLY GELTHKKSRQ 1390 1400 1410 1420 1430 1440 ADDSSLFPLS IQLKVRGRGF LQGLLDGLMR SSCCQVIHRR SVSTLKRPAN AVYGGLIYLI 1450 1460 1470 1480 1490 1500 DKLSASSPFL SLVRTGPIRQ ELEQVPHKMS TSYPTNIRDL GSIVRNYFKY QCRPVERGHY 1510 1520 1530 1540 1550 1560 KTYYNQIWLF SDVLSTEFIG PMRISSSLLK LLYRSSLTKK DKEELRELAA LSSNLRSGED 1570 1580 1590 1600 1610 1620 WDDLHIKFFS NDLLFCPAEI RHACKFGIQK ENEDIALYPN WGIEYIGNVI DIPVFYRAQN 1630 1640 1650 1660 1670 1680 VQKDIRIPLR IQNPLMSGLR LGQLPTGAHY KMRTIISRLK IPYHDFLACG DGSGGMTVAL 1690 1700 1710 1720 1730 1740 LRLNRASRGI FNSLLDLSDT MLRGSSPEPP SALETLGGER SRCVNGDSCW EHPSDLSDKN 1750 1760 1770 1780 1790 1800 TWKYFLHVKK GCGMSINLIT MDMEVKDPTM SYKIELLVRQ YVPVLLESDC CLIYKTYGTY 1810 1820 1830 1840 1850 1860 IATQKDNSLT LIGSLFHSVQ LVQTDLSSSN TSELYLVCRR LKDYIDTPFV DWIELYDSWE 1870 1880 1890 1900 1910 1920 NQYAFKDIKD EFHRARSLKP ETTLVGIPPQ FIPDPGVNLE TLFQIAGVPT GVAHGVIITS 1930 1940 1950 1960 1970 1980 MQSKNKLISN AIGSMCVISH FVMNTIRTTD SMPGPPSDGD VNKMCSALIG TCFWLSWMES 1990 2000 2010 2020 2030 2040 DLNLYKTCLR SIMKSMPVRW FRALKNGKWF QKWDCKGDAI PKDSRLGDSM ANIGNWIRAW 2050 2060 2070 2080 2090 2100 ELIRDGNISE PFDAAAVEML TTSVDKSLSW KKILKTTGIP RLLNSDTDVI DQSILNVQID IVENQAWQN

References

[1]	"Nucleotide sequence of the L gene of vesicular stomatitis virus (New Jersey): identification of conserved domains in the New Jersey and Indiana L proteins." Feldhaus A.L., Lesnaw J.A. Virology 163:359-368(1988) [PubMed: 2833012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M20166 Genomic RNA. Translation: AAA48440.1.
PIR	ZLVNNJ. A28602.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR017234. RNA-dir_RNA_pol_rhabdovir. IPR014023. RNA_pol_cat. IPR001016. RNA_pol_L_viral. [Graphical view]
Pfam	PF00946. Paramyx_RNA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF037546. RNA_pol_RhabdoV_sub. 1 hit.
PROSITE	PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_VSNJH

Accession

Primary (citable) accession number: P13615

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	June 16, 2009
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)