P13607 (ATNA_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sodium/potassium-transporting ATPase subunit alpha
Short name=Na(+)/K(+) ATPase alpha subunit
EC=3.6.3.9

Alternative name(s):
Sodium pump subunit alpha

Gene names

Name:	Atpalpha
Synonyms:	Na-p
ORF Names:	CG5670

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	1041 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. Ref.1 Ref.2 Ref.6
Catalytic activity	ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In).
Subunit structure	Composed of three subunits: alpha (catalytic), beta and gamma.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	High levels are found in some adult tissues: Malpighian tubules, indirect flight muscles, tubular leg muscles and throughout the nervous system (brain, optic lobes, retina and ventral thoracic neuromere). Lower levels are detected at the posterior end where the reproductive organs and rectum are located. Ref.1 Ref.2
Miscellaneous	Ouabain-sensitive electrogenic ion pump.
Sequence similarities	Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily. [View classification]
RNA editing	Edited at position 429. Partially edited. Target of Adar. Ref.5 Ref.9

Ontologies

Keywords
Biological process	Ion transport Potassium transport Sodium transport Sodium/potassium transport Transport
Cellular component	Membrane
Coding sequence diversity	Alternative splicing RNA editing
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding Potassium Sodium
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro adult locomotory behavior Inferred from mutant phenotype Ref.6. Source: FlyBase determination of adult lifespan Inferred from mutant phenotype Ref.6PubMed 16272407. Source: FlyBase embryonic development via the syncytial blastoderm Inferred from mutant phenotype PubMed 8978055. Source: FlyBase neuromuscular process Inferred from mutant phenotype Ref.6. Source: FlyBase regulation of cell shape Inferred from mutant phenotype PubMed 12466193. Source: FlyBase regulation of tube diameter, open tracheal system Inferred from mutant phenotype PubMed 12466193PubMed 12930776. Source: FlyBase regulation of tube length, open tracheal system Inferred from mutant phenotype PubMed 12466193PubMed 12930776. Source: FlyBase response to mechanical stimulus Inferred from mutant phenotype PubMed 11691629Ref.6PubMed 8110464. Source: FlyBase response to temperature stimulus Inferred from mutant phenotype Ref.6Ref.7. Source: FlyBase septate junction assembly Inferred from mutant phenotype PubMed 12930776. Source: FlyBase synaptic growth at neuromuscular junction Inferred from mutant phenotype PubMed 15281071. Source: FlyBase synaptic transmission Inferred from direct assay PubMed 15281071. Source: FlyBase tissue homeostasis Inferred from mutant phenotype Ref.6. Source: FlyBase
Cellular_component	basolateral plasma membrane Inferred from direct assay PubMed 22417167. Source: FlyBase nucleus Inferred from direct assay PubMed 8110464. Source: FlyBase septate junction Inferred from direct assay PubMed 12782686PubMed 12930776. Source: FlyBase sodium:potassium-exchanging ATPase complex Inferred by curator Ref.2. Source: FlyBase
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: InterPro sodium:potassium-exchanging ATPase activity Inferred from mutant phenotype PubMed 8110464Ref.7. Source: FlyBase
Complete GO annotation...

Alternative products

This entry describes 7 isoforms produced by alternative splicing. [Align] [Select]

Note: Exon 6 has 4 mutually exclusive forms (6a, 6b, 6c and 6d). Additional isoforms may exist.

Isoform 1 (identifier: P13607-1)

Also known as: A;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P13607-2)

Also known as: B; C; E;

The sequence of this isoform differs from the canonical sequence as follows:
     1-39: Missing.

Isoform 3 (identifier: P13607-3)

Also known as: D;

The sequence of this isoform differs from the canonical sequence as follows:
     1-39: Missing.
     836-878: PAISLAYEHA...SMAYGQIGMI → SLDLCPKPKL...CSPSQLIVKN
     879-1041: Missing.

Note: No experimental confirmation available.

Isoform 4 (identifier: P13607-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-8: MALRSDYE → MSAQ

Isoform 5 (identifier: P13607-5)

The sequence of this isoform differs from the canonical sequence as follows:
     835-865: VPAISLAYEHAEADIMKRPPRDPFNDKLVNS → IPAISLAYEGPEADIMKRRPRNPEIDNLVNE

Isoform 6 (identifier: P13607-6)

Also known as: F; H;

The sequence of this isoform differs from the canonical sequence as follows:
     1-39: Missing.
     844-865: HAEADIMKRPPRDPFNDKLVNS → TAESDIMKRQPRNPFQDKLVNE

Isoform 7 (identifier: P13607-7)

Also known as: G;

The sequence of this isoform differs from the canonical sequence as follows:
     1-39: Missing.
     835-865: VPAISLAYEHAEADIMKRPPRDPFNDKLVNS → IPAISLAYEQAESDIMKRQPRDPYRDNLVNR

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1041	1041	Sodium/potassium-transporting ATPase subunit alpha		PRO_0000046309
Regions
	Transmembrane	115 – 135	21	Helical; Potential
	Transmembrane	147 – 167	21	Helical; Potential
	Transmembrane	312 – 332	21	Helical; Potential
	Transmembrane	338 – 358	21	Helical; Potential
	Transmembrane	808 – 828	21	Helical; Potential
	Transmembrane	870 – 890	21	Helical; Potential
	Transmembrane	935 – 955	21	Helical; Potential
	Transmembrane	970 – 990	21	Helical; Potential
Sites
	Active site	394	1	4-aspartylphosphate intermediate Probable
	Binding site	526	1	ATP By similarity
Natural variations
	Alternative sequence	1 – 39	39	Missing in isoform 2, isoform 3, isoform 6 and isoform 7.		VSP_000417
	Alternative sequence	1 – 8	8	MALRSDYE → MSAQ in isoform 4.		VSP_008074
	Alternative sequence	835 – 865	31	VPAIS…KLVNS → IPAISLAYEGPEADIMKRRP RNPEIDNLVNE in isoform 5.		VSP_008075
	Alternative sequence	835 – 865	31	VPAIS…KLVNS → IPAISLAYEQAESDIMKRQP RDPYRDNLVNR in isoform 7.		VSP_008076
	Alternative sequence	836 – 878	43	PAISL…QIGMI → SLDLCPKPKLHRRLKLKLLL SQYHSSKLYSYTSCSPSQLI VKN in isoform 3.		VSP_008077
	Alternative sequence	844 – 865	22	HAEAD…KLVNS → TAESDIMKRQPRNPFQDKLV NE in isoform 6.		VSP_000418
	Alternative sequence	879 – 1041	163	Missing in isoform 3.		VSP_008078
	Natural variant	429	1	Y → C in RNA edited version.
Experimental info
	Mutagenesis	1020	1	D → N in allele ATP-alpha-dts2; homozygous lethal and temperature dependent bang sensitive paralysis, shortened life span and neurodegeneration when heterozygous. Ref.6
	Mutagenesis	1021	1	E → K in allele ATP-alpha-dts1; homozygous lethal and temperature dependent bang sensitive paralysis, shortened life span and neurodegeneration when heterozygous. Ref.6
	Sequence conflict	69	1	L → M in CAA32638. Ref.1
	Sequence conflict	85	1	K → R in CAA32638. Ref.1
	Sequence conflict	97	1	Missing in CAA32638. Ref.1
	Sequence conflict	112 – 113	2	KN → ED in CAA32638. Ref.1
	Sequence conflict	117 – 118	2	GF → V in CAA32638. Ref.1
	Sequence conflict	163	1	I → V in CAA32638. Ref.1
	Sequence conflict	192	1	E → G in AAC05260. Ref.2
	Sequence conflict	196 – 197	2	LT → PS in CAA32638. Ref.1
	Sequence conflict	207 – 208	2	DV → VL in CAA32638. Ref.1
	Sequence conflict	211 – 212	2	VK → LE in CAA32638. Ref.1
	Sequence conflict	216 – 221	6	RIPADI → LIPLVY in CAA32638. Ref.1
	Sequence conflict	228	1	N → D in CAA32638. Ref.1
	Sequence conflict	270 – 272	3	GTA → ALP in CAA32638. Ref.1
	Sequence conflict	290	1	G → A in CAA32638. Ref.1
	Sequence conflict	299	1	Missing in CAA32638. Ref.1
	Sequence conflict	402	1	N → T in CAA35504. Ref.8
	Sequence conflict	488	1	I → N in CAA35504. Ref.8
	Sequence conflict	811	1	F → S in CAA32638. Ref.1
	Sequence conflict	843	1	E → D in CAA32638. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (A) [UniParc]. Last modified June 20, 2001. Version 3. Checksum: B2DD36B2E9029F43	FASTA	1,041	115,605
	10 20 30 40 50 60 MALRSDYEHG RADSYRVATV IATDDDNRTA DGQYKSRRKM PAKVNKKENL DDLKQELDID 70 80 90 100 110 120 FHKISPEELY QRFQTHPENG LSHAKAKENL ERDGPNALTP PKQTPEWVKF CKNLFGGFAM 130 140 150 160 170 180 LLWIGAILCF VAYSIQASTS EEPADDNLYL GIVLSAVVIV TGIFSYYQES KSSKIMESFK 190 200 210 220 230 240 NMVPQFATVI REGEKLTLRA EDLVLGDVVE VKFGDRIPAD IRIIEARNFK VDNSSLTGES 250 260 270 280 290 300 EPQSRGAEFT HENPLETKNL AFFSTNAVEG TAKGVVISCG DHTVMGRIAG LASGLDTGET 310 320 330 340 350 360 PIAKEIHHFI HLITGVAVFL GVTFFVIAFI LGYHWLDAVI FLIGIIVANV PEGLLATVTV 370 380 390 400 410 420 CLTLTAKRMA SKNCLVKNLE AVETLGSTST ICSDKTGTLT QNRMTVAHMW FDNQIIEADT 430 440 450 460 470 480 TEDQSGVQYD RTSPGFKALS RIATLCNRAE FKGGQDGVPI LKKEVSGDAS EAALLKCMEL 490 500 510 520 530 540 ALGDVMNIRK RNKKIAEVPF NSTNKYQVSI HETEDTNDPR YLLVMKGAPE RILERCSTIF 550 560 570 580 590 600 INGKEKVLDE EMKEAFNNAY MELGGLGERV LGFCDFMLPS DKYPNGFKFN TDDINFPIDN 610 620 630 640 650 660 LRFVGLMSMI DPPRAAVPDA VAKCRSAGIK VIMVTGDHPI TAKAIAKSVG IISEGNETVE 670 680 690 700 710 720 DIAQRLNIPV SEVNPREAKA AVVHGAELRD VSSDQLDEIL RYHTEIVFAR TSPQQKLIIV 730 740 750 760 770 780 EGCQRMGAIV AVTGDGVNDS PALKKADIGV AMGIAGSDVS KQAADMILLD DNFASIVTGV 790 800 810 820 830 840 EEGRLIFDNL KKSIAYTLTS NIPEISPFLA FILCDIPLPL GTVTILCIDL GTDMVPAISL 850 860 870 880 890 900 AYEHAEADIM KRPPRDPFND KLVNSRLISM AYGQIGMIQA AAGFFVYFVI MAENGFLPKK 910 920 930 940 950 960 LFGIRKMWDS KAVNDLTDSY GQEWTYRDRK TLEYTCHTAF FISIVVVQWA DLIICKTRRN 970 980 990 1000 1010 1020 SIFQQGMRNW ALNFGLVFET VLAAFLSYCP GMEKGLRMYP LKLVWWFPAI PFALAIFIYD 1030 1040 ETRRFYLRRN PGGWLEQETY Y
	Isoform 2 (B) (C) (E) [UniParc]. Checksum: CA77B36B10EB23E2	FASTA	1,002	111,133
	10 20 30 40 50 60 MPAKVNKKEN LDDLKQELDI DFHKISPEEL YQRFQTHPEN GLSHAKAKEN LERDGPNALT 70 80 90 100 110 120 PPKQTPEWVK FCKNLFGGFA MLLWIGAILC FVAYSIQAST SEEPADDNLY LGIVLSAVVI 130 140 150 160 170 180 VTGIFSYYQE SKSSKIMESF KNMVPQFATV IREGEKLTLR AEDLVLGDVV EVKFGDRIPA 190 200 210 220 230 240 DIRIIEARNF KVDNSSLTGE SEPQSRGAEF THENPLETKN LAFFSTNAVE GTAKGVVISC 250 260 270 280 290 300 GDHTVMGRIA GLASGLDTGE TPIAKEIHHF IHLITGVAVF LGVTFFVIAF ILGYHWLDAV 310 320 330 340 350 360 IFLIGIIVAN VPEGLLATVT VCLTLTAKRM ASKNCLVKNL EAVETLGSTS TICSDKTGTL 370 380 390 400 410 420 TQNRMTVAHM WFDNQIIEAD TTEDQSGVQY DRTSPGFKAL SRIATLCNRA EFKGGQDGVP 430 440 450 460 470 480 ILKKEVSGDA SEAALLKCME LALGDVMNIR KRNKKIAEVP FNSTNKYQVS IHETEDTNDP 490 500 510 520 530 540 RYLLVMKGAP ERILERCSTI FINGKEKVLD EEMKEAFNNA YMELGGLGER VLGFCDFMLP 550 560 570 580 590 600 SDKYPNGFKF NTDDINFPID NLRFVGLMSM IDPPRAAVPD AVAKCRSAGI KVIMVTGDHP 610 620 630 640 650 660 ITAKAIAKSV GIISEGNETV EDIAQRLNIP VSEVNPREAK AAVVHGAELR DVSSDQLDEI 670 680 690 700 710 720 LRYHTEIVFA RTSPQQKLII VEGCQRMGAI VAVTGDGVND SPALKKADIG VAMGIAGSDV 730 740 750 760 770 780 SKQAADMILL DDNFASIVTG VEEGRLIFDN LKKSIAYTLT SNIPEISPFL AFILCDIPLP 790 800 810 820 830 840 LGTVTILCID LGTDMVPAIS LAYEHAEADI MKRPPRDPFN DKLVNSRLIS MAYGQIGMIQ 850 860 870 880 890 900 AAAGFFVYFV IMAENGFLPK KLFGIRKMWD SKAVNDLTDS YGQEWTYRDR KTLEYTCHTA 910 920 930 940 950 960 FFISIVVVQW ADLIICKTRR NSIFQQGMRN WALNFGLVFE TVLAAFLSYC PGMEKGLRMY 970 980 990 1000 PLKLVWWFPA IPFALAIFIY DETRRFYLRR NPGGWLEQET YY
	Isoform 3 (D) [UniParc]. Checksum: D8D38F5B5BE2CD37	FASTA	839	91,981
	10 20 30 40 50 60 MPAKVNKKEN LDDLKQELDI DFHKISPEEL YQRFQTHPEN GLSHAKAKEN LERDGPNALT 70 80 90 100 110 120 PPKQTPEWVK FCKNLFGGFA MLLWIGAILC FVAYSIQAST SEEPADDNLY LGIVLSAVVI 130 140 150 160 170 180 VTGIFSYYQE SKSSKIMESF KNMVPQFATV IREGEKLTLR AEDLVLGDVV EVKFGDRIPA 190 200 210 220 230 240 DIRIIEARNF KVDNSSLTGE SEPQSRGAEF THENPLETKN LAFFSTNAVE GTAKGVVISC 250 260 270 280 290 300 GDHTVMGRIA GLASGLDTGE TPIAKEIHHF IHLITGVAVF LGVTFFVIAF ILGYHWLDAV 310 320 330 340 350 360 IFLIGIIVAN VPEGLLATVT VCLTLTAKRM ASKNCLVKNL EAVETLGSTS TICSDKTGTL 370 380 390 400 410 420 TQNRMTVAHM WFDNQIIEAD TTEDQSGVQY DRTSPGFKAL SRIATLCNRA EFKGGQDGVP 430 440 450 460 470 480 ILKKEVSGDA SEAALLKCME LALGDVMNIR KRNKKIAEVP FNSTNKYQVS IHETEDTNDP 490 500 510 520 530 540 RYLLVMKGAP ERILERCSTI FINGKEKVLD EEMKEAFNNA YMELGGLGER VLGFCDFMLP 550 560 570 580 590 600 SDKYPNGFKF NTDDINFPID NLRFVGLMSM IDPPRAAVPD AVAKCRSAGI KVIMVTGDHP 610 620 630 640 650 660 ITAKAIAKSV GIISEGNETV EDIAQRLNIP VSEVNPREAK AAVVHGAELR DVSSDQLDEI 670 680 690 700 710 720 LRYHTEIVFA RTSPQQKLII VEGCQRMGAI VAVTGDGVND SPALKKADIG VAMGIAGSDV 730 740 750 760 770 780 SKQAADMILL DDNFASIVTG VEEGRLIFDN LKKSIAYTLT SNIPEISPFL AFILCDIPLP 790 800 810 820 830 LGTVTILCID LGTDMVSLDL CPKPKLHRRL KLKLLLSQYH SSKLYSYTSC SPSQLIVKN
	Isoform 4 [UniParc]. Checksum: 62722EDBA74BBC88	FASTA	1,037	115,056
	10 20 30 40 50 60 MSAQHGRADS YRVATVIATD DDNRTADGQY KSRRKMPAKV NKKENLDDLK QELDIDFHKI 70 80 90 100 110 120 SPEELYQRFQ THPENGLSHA KAKENLERDG PNALTPPKQT PEWVKFCKNL FGGFAMLLWI 130 140 150 160 170 180 GAILCFVAYS IQASTSEEPA DDNLYLGIVL SAVVIVTGIF SYYQESKSSK IMESFKNMVP 190 200 210 220 230 240 QFATVIREGE KLTLRAEDLV LGDVVEVKFG DRIPADIRII EARNFKVDNS SLTGESEPQS 250 260 270 280 290 300 RGAEFTHENP LETKNLAFFS TNAVEGTAKG VVISCGDHTV MGRIAGLASG LDTGETPIAK 310 320 330 340 350 360 EIHHFIHLIT GVAVFLGVTF FVIAFILGYH WLDAVIFLIG IIVANVPEGL LATVTVCLTL 370 380 390 400 410 420 TAKRMASKNC LVKNLEAVET LGSTSTICSD KTGTLTQNRM TVAHMWFDNQ IIEADTTEDQ 430 440 450 460 470 480 SGVQYDRTSP GFKALSRIAT LCNRAEFKGG QDGVPILKKE VSGDASEAAL LKCMELALGD 490 500 510 520 530 540 VMNIRKRNKK IAEVPFNSTN KYQVSIHETE DTNDPRYLLV MKGAPERILE RCSTIFINGK 550 560 570 580 590 600 EKVLDEEMKE AFNNAYMELG GLGERVLGFC DFMLPSDKYP NGFKFNTDDI NFPIDNLRFV 610 620 630 640 650 660 GLMSMIDPPR AAVPDAVAKC RSAGIKVIMV TGDHPITAKA IAKSVGIISE GNETVEDIAQ 670 680 690 700 710 720 RLNIPVSEVN PREAKAAVVH GAELRDVSSD QLDEILRYHT EIVFARTSPQ QKLIIVEGCQ 730 740 750 760 770 780 RMGAIVAVTG DGVNDSPALK KADIGVAMGI AGSDVSKQAA DMILLDDNFA SIVTGVEEGR 790 800 810 820 830 840 LIFDNLKKSI AYTLTSNIPE ISPFLAFILC DIPLPLGTVT ILCIDLGTDM VPAISLAYEH 850 860 870 880 890 900 AEADIMKRPP RDPFNDKLVN SRLISMAYGQ IGMIQAAAGF FVYFVIMAEN GFLPKKLFGI 910 920 930 940 950 960 RKMWDSKAVN DLTDSYGQEW TYRDRKTLEY TCHTAFFISI VVVQWADLII CKTRRNSIFQ 970 980 990 1000 1010 1020 QGMRNWALNF GLVFETVLAA FLSYCPGMEK GLRMYPLKLV WWFPAIPFAL AIFIYDETRR 1030 FYLRRNPGGW LEQETYY
	Isoform 5 [UniParc]. Checksum: D6084967A87F4FAD	FASTA	1,041	115,632
	10 20 30 40 50 60 MALRSDYEHG RADSYRVATV IATDDDNRTA DGQYKSRRKM PAKVNKKENL DDLKQELDID 70 80 90 100 110 120 FHKISPEELY QRFQTHPENG LSHAKAKENL ERDGPNALTP PKQTPEWVKF CKNLFGGFAM 130 140 150 160 170 180 LLWIGAILCF VAYSIQASTS EEPADDNLYL GIVLSAVVIV TGIFSYYQES KSSKIMESFK 190 200 210 220 230 240 NMVPQFATVI REGEKLTLRA EDLVLGDVVE VKFGDRIPAD IRIIEARNFK VDNSSLTGES 250 260 270 280 290 300 EPQSRGAEFT HENPLETKNL AFFSTNAVEG TAKGVVISCG DHTVMGRIAG LASGLDTGET 310 320 330 340 350 360 PIAKEIHHFI HLITGVAVFL GVTFFVIAFI LGYHWLDAVI FLIGIIVANV PEGLLATVTV 370 380 390 400 410 420 CLTLTAKRMA SKNCLVKNLE AVETLGSTST ICSDKTGTLT QNRMTVAHMW FDNQIIEADT 430 440 450 460 470 480 TEDQSGVQYD RTSPGFKALS RIATLCNRAE FKGGQDGVPI LKKEVSGDAS EAALLKCMEL 490 500 510 520 530 540 ALGDVMNIRK RNKKIAEVPF NSTNKYQVSI HETEDTNDPR YLLVMKGAPE RILERCSTIF 550 560 570 580 590 600 INGKEKVLDE EMKEAFNNAY MELGGLGERV LGFCDFMLPS DKYPNGFKFN TDDINFPIDN 610 620 630 640 650 660 LRFVGLMSMI DPPRAAVPDA VAKCRSAGIK VIMVTGDHPI TAKAIAKSVG IISEGNETVE 670 680 690 700 710 720 DIAQRLNIPV SEVNPREAKA AVVHGAELRD VSSDQLDEIL RYHTEIVFAR TSPQQKLIIV 730 740 750 760 770 780 EGCQRMGAIV AVTGDGVNDS PALKKADIGV AMGIAGSDVS KQAADMILLD DNFASIVTGV 790 800 810 820 830 840 EEGRLIFDNL KKSIAYTLTS NIPEISPFLA FILCDIPLPL GTVTILCIDL GTDMIPAISL 850 860 870 880 890 900 AYEGPEADIM KRRPRNPEID NLVNERLISM AYGQIGMIQA AAGFFVYFVI MAENGFLPKK 910 920 930 940 950 960 LFGIRKMWDS KAVNDLTDSY GQEWTYRDRK TLEYTCHTAF FISIVVVQWA DLIICKTRRN 970 980 990 1000 1010 1020 SIFQQGMRNW ALNFGLVFET VLAAFLSYCP GMEKGLRMYP LKLVWWFPAI PFALAIFIYD 1030 1040 ETRRFYLRRN PGGWLEQETY Y
	Isoform 6 (F) (H) [UniParc]. Checksum: 4146F52C60C4114A	FASTA	1,002	111,199
	10 20 30 40 50 60 MPAKVNKKEN LDDLKQELDI DFHKISPEEL YQRFQTHPEN GLSHAKAKEN LERDGPNALT 70 80 90 100 110 120 PPKQTPEWVK FCKNLFGGFA MLLWIGAILC FVAYSIQAST SEEPADDNLY LGIVLSAVVI 130 140 150 160 170 180 VTGIFSYYQE SKSSKIMESF KNMVPQFATV IREGEKLTLR AEDLVLGDVV EVKFGDRIPA 190 200 210 220 230 240 DIRIIEARNF KVDNSSLTGE SEPQSRGAEF THENPLETKN LAFFSTNAVE GTAKGVVISC 250 260 270 280 290 300 GDHTVMGRIA GLASGLDTGE TPIAKEIHHF IHLITGVAVF LGVTFFVIAF ILGYHWLDAV 310 320 330 340 350 360 IFLIGIIVAN VPEGLLATVT VCLTLTAKRM ASKNCLVKNL EAVETLGSTS TICSDKTGTL 370 380 390 400 410 420 TQNRMTVAHM WFDNQIIEAD TTEDQSGVQY DRTSPGFKAL SRIATLCNRA EFKGGQDGVP 430 440 450 460 470 480 ILKKEVSGDA SEAALLKCME LALGDVMNIR KRNKKIAEVP FNSTNKYQVS IHETEDTNDP 490 500 510 520 530 540 RYLLVMKGAP ERILERCSTI FINGKEKVLD EEMKEAFNNA YMELGGLGER VLGFCDFMLP 550 560 570 580 590 600 SDKYPNGFKF NTDDINFPID NLRFVGLMSM IDPPRAAVPD AVAKCRSAGI KVIMVTGDHP 610 620 630 640 650 660 ITAKAIAKSV GIISEGNETV EDIAQRLNIP VSEVNPREAK AAVVHGAELR DVSSDQLDEI 670 680 690 700 710 720 LRYHTEIVFA RTSPQQKLII VEGCQRMGAI VAVTGDGVND SPALKKADIG VAMGIAGSDV 730 740 750 760 770 780 SKQAADMILL DDNFASIVTG VEEGRLIFDN LKKSIAYTLT SNIPEISPFL AFILCDIPLP 790 800 810 820 830 840 LGTVTILCID LGTDMVPAIS LAYETAESDI MKRQPRNPFQ DKLVNERLIS MAYGQIGMIQ 850 860 870 880 890 900 AAAGFFVYFV IMAENGFLPK KLFGIRKMWD SKAVNDLTDS YGQEWTYRDR KTLEYTCHTA 910 920 930 940 950 960 FFISIVVVQW ADLIICKTRR NSIFQQGMRN WALNFGLVFE TVLAAFLSYC PGMEKGLRMY 970 980 990 1000 PLKLVWWFPA IPFALAIFIY DETRRFYLRR NPGGWLEQET YY
	Isoform 7 (G) [UniParc]. Checksum: 9762610EA4A85D45	FASTA	1,002	111,298
	10 20 30 40 50 60 MPAKVNKKEN LDDLKQELDI DFHKISPEEL YQRFQTHPEN GLSHAKAKEN LERDGPNALT 70 80 90 100 110 120 PPKQTPEWVK FCKNLFGGFA MLLWIGAILC FVAYSIQAST SEEPADDNLY LGIVLSAVVI 130 140 150 160 170 180 VTGIFSYYQE SKSSKIMESF KNMVPQFATV IREGEKLTLR AEDLVLGDVV EVKFGDRIPA 190 200 210 220 230 240 DIRIIEARNF KVDNSSLTGE SEPQSRGAEF THENPLETKN LAFFSTNAVE GTAKGVVISC 250 260 270 280 290 300 GDHTVMGRIA GLASGLDTGE TPIAKEIHHF IHLITGVAVF LGVTFFVIAF ILGYHWLDAV 310 320 330 340 350 360 IFLIGIIVAN VPEGLLATVT VCLTLTAKRM ASKNCLVKNL EAVETLGSTS TICSDKTGTL 370 380 390 400 410 420 TQNRMTVAHM WFDNQIIEAD TTEDQSGVQY DRTSPGFKAL SRIATLCNRA EFKGGQDGVP 430 440 450 460 470 480 ILKKEVSGDA SEAALLKCME LALGDVMNIR KRNKKIAEVP FNSTNKYQVS IHETEDTNDP 490 500 510 520 530 540 RYLLVMKGAP ERILERCSTI FINGKEKVLD EEMKEAFNNA YMELGGLGER VLGFCDFMLP 550 560 570 580 590 600 SDKYPNGFKF NTDDINFPID NLRFVGLMSM IDPPRAAVPD AVAKCRSAGI KVIMVTGDHP 610 620 630 640 650 660 ITAKAIAKSV GIISEGNETV EDIAQRLNIP VSEVNPREAK AAVVHGAELR DVSSDQLDEI 670 680 690 700 710 720 LRYHTEIVFA RTSPQQKLII VEGCQRMGAI VAVTGDGVND SPALKKADIG VAMGIAGSDV 730 740 750 760 770 780 SKQAADMILL DDNFASIVTG VEEGRLIFDN LKKSIAYTLT SNIPEISPFL AFILCDIPLP 790 800 810 820 830 840 LGTVTILCID LGTDMIPAIS LAYEQAESDI MKRQPRDPYR DNLVNRRLIS MAYGQIGMIQ 850 860 870 880 890 900 AAAGFFVYFV IMAENGFLPK KLFGIRKMWD SKAVNDLTDS YGQEWTYRDR KTLEYTCHTA 910 920 930 940 950 960 FFISIVVVQW ADLIICKTRR NSIFQQGMRN WALNFGLVFE TVLAAFLSYC PGMEKGLRMY 970 980 990 1000 PLKLVWWFPA IPFALAIFIY DETRRFYLRR NPGGWLEQET YY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization and expression of the (Na+ + K+)-ATPase alpha-subunit in Drosophila melanogaster." Lebovitz R.M., Takeyasu K., Fambrough D.M. EMBO J. 8:193-202(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY. Strain: Canton-S and Oregon-R. Tissue: Embryo, Head and Pupae.
[2]	"Functional analysis and tissue-specific expression of Drosophila Na+,K+-ATPase subunits." Sun B., Wang W., Salvaterra P.M. J. Neurochem. 71:142-151(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, TISSUE SPECIFICITY. Tissue: Head.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), RNA EDITING OF POSITION 429. Strain: Berkeley. Tissue: Head.
[6]	"Neural dysfunction and neurodegeneration in Drosophila Na+/K+ ATPase alpha subunit mutants." Palladino M.J., Bower J.E., Kreber R., Ganetzky B. J. Neurosci. 23:1276-1286(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-35 (ISOFORM 4), NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 835-865 (ISOFORMS 1; 5; 6 AND 7), FUNCTION, MUTAGENESIS OF ASP-1020 AND GLU-1021.
[7]	"The Drosophila Na,K-ATPase alpha-subunit gene: gene structure, promoter function and analysis of a cold-sensitive recessive-lethal mutation." Feng Y., Huynh L., Takeyasu K., Fambrough D.M. Genes Funct. 1:99-117(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-8.
[8]	"Amplification of the phosphorylation site-ATP-binding site cDNA fragment of the Na+,K(+)-ATPase and the Ca2(+)-ATPase of Drosophila melanogaster by polymerase chain reaction." Varadi A., Gilmore-Heber M., Benz E.J. Jr. FEBS Lett. 258:203-207(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 400-524.
[9]	"RNA editing in Drosophila melanogaster: new targets and functional consequences." Stapleton M., Carlson J.W., Celniker S.E. RNA 12:1922-1932(2006) [PubMed] [Europe PMC] [Abstract] Cited for: RNA EDITING OF POSITION 429.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X14476 mRNA. Translation: CAA32638.1. AF044974 mRNA. Translation: AAC05260.1. AE014297 Genomic DNA. Translation: AAF55825.3. AE014297 Genomic DNA. Translation: AAF55826.2. AE014297 Genomic DNA. Translation: AAF55828.1. AE014297 Genomic DNA. Translation: AAS65183.1. AE014297 Genomic DNA. Translation: AAS65184.1. AE014297 Genomic DNA. Translation: AAS65185.1. AE014297 Genomic DNA. Translation: AAS65186.1. AY069184 mRNA. Translation: AAL39329.1. AY174097 Genomic DNA. Translation: AAO33930.1. AY174097 Genomic DNA. Translation: AAO33931.1. AY174097 Genomic DNA. Translation: AAO33932.1. AY174097 Genomic DNA. Translation: AAO33933.1. AY174098 Genomic DNA. Translation: AAO33929.1. U55767 Genomic DNA. Translation: AAB01189.1. X17471 mRNA. Translation: CAA35504.1.
PIR	S03632.
RefSeq	NP_001262790.1. NM_001275861.1. NP_001262791.1. NM_001275862.1. NP_732572.1. NM_169936.2. NP_732573.1. NM_169937.2. NP_732574.1. NM_169938.2. NP_732575.1. NM_169939.2. NP_996247.1. NM_206525.2. NP_996248.1. NM_206526.2. NP_996249.1. NM_206527.2. NP_996250.1. NM_206528.2.
UniGene	Dm.6725.
3D structure databases
ProteinModelPortal	P13607.
SMR	P13607. Positions 50-1041.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-19649N.
IntAct	P13607. 2 interactions.
MINT	MINT-284024.
STRING	7227.FBpp0088502.
Proteomic databases
PaxDb	P13607.
PRIDE	P13607.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0089510; FBpp0088502; FBgn0002921.
GeneID	48971.
KEGG	dme:Dmel_CG5670.
Organism-specific databases
CTD	48971.
FlyBase	FBgn0002921. Atpalpha.
Phylogenomic databases
eggNOG	COG0474.
GeneTree	ENSGT00560000076887.
InParanoid	P13607.
KO	K01539.
OMA	FQTHPEN.
OrthoDB	EOG4QZ61S.
PhylomeDB	P13607.
Gene expression databases
Bgee	P13607.
GermOnline	CG5670. Drosophila melanogaster.
Family and domain databases
Gene3D	1.20.1110.10. 2 hits. 2.70.150.10. 2 hits. 3.40.1110.10. 1 hit.
InterPro	IPR006068. ATPase_P-typ_cation-transptr_C. IPR004014. ATPase_P-typ_cation-transptr_N. IPR023299. ATPase_P-typ_cyto_domN. IPR005775. ATPase_P-typ_Na/K_IIC. IPR018303. ATPase_P-typ_P_site. IPR023298. ATPase_P-typ_TM_dom. IPR008250. ATPase_P-typ_transduc_dom_A. IPR001757. Cation_transp_P_typ_ATPase. IPR023214. HAD-like_dom. [Graphical view]
PANTHER	PTHR24093. PTHR24093. 1 hit.
Pfam	PF00689. Cation_ATPase_C. 1 hit. PF00690. Cation_ATPase_N. 1 hit. PF00122. E1-E2_ATPase. 1 hit. PF00702. Hydrolase. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE.
SMART	SM00831. Cation_ATPase_N. 1 hit. [Graphical view]
SUPFAM	SSF81660. ATPase_cation_domN. 1 hit. SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01106. ATPase-IIC_X-K. 1 hit. TIGR01494. ATPase_P-type. 2 hits.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	Atpalpha. drosophila.
GenomeRNAi	48971.
NextBio	839577.

Entry information

Entry name

ATNA_DROME

Accession

Primary (citable) accession number: P13607
Secondary accession number(s): A4V366 , A4V367, O61494, Q0KI37, Q7KS94, Q7KS95, Q86MY6, Q86MY7, Q86MY8, Q86MY9, Q86MZ0, Q8IN40, Q8T0L8, Q9VDG6, Q9VDG7, Q9VDG8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	June 20, 2001
Last modified:	May 1, 2013
This is version 145 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project