Reviewed, UniProtKB/Swiss-Prot P13604 (ADH1_CLOSA)

Names and origin

Protein names

Recommended name:
    NADPH-dependent butanol dehydrogenase
      Short name=BDH
    EC=1.1.1.-

Gene names

Name:

adh1

Organism

Clostridium saccharobutylicum

Taxonomic identifier

169679 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This enzyme has activity using butanol and ethanol as substrates.
Sequence similarities	Belongs to the iron-containing alcohol dehydrogenase family.
Caution	Was originally (Ref.1) thought to originate from C.acetobutylicum.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 388	388	NADPH-dependent butanol dehydrogenase		PRO_0000087821

Sequences

	Sequence		Length	Mass (Da)
	P13604-1 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: BBFDD458BFAB2C1B	FASTA	388	43,274
	10 20 30 40 50 60 MMRFTLPRDI YYGKGSLEQL KNLKGKKAML VLGGGSMKRF GFVDKVLGYL KEAGIEVKLI 70 80 90 100 110 120 EGVEPDPSVE TVFKGAELMR QFEPDWIIAM GGGSPIDAAK AMWIFYEHPE KTFDDIKDPF 130 140 150 160 170 180 TVPELRNKAK FLAIPSTSGT ATEVTAFSVI TDYKTEIKYP LADFNITPDV AVVDSELAET 190 200 210 220 230 240 MPPKLTAHTG MDALTHAIEA YVATLHSPFT DPLAMQAIEM INEHLFKSYE GDKEAREQMH 250 260 270 280 290 300 YAQCLAGMAF SNALLGICHS MAHKTGAVFH IPHGCANAIY LPYVIKFNSK TSLERYAKIA 310 320 330 340 350 360 KQISLAGNTN EELVDSLINL VKELNKKMQI PTTLKEYGIH EQEFKNKVDL ISERAIGDAC 370 380 TGSNPRQLNK DEMKKIFECV YYGTEVDF

References

[1]

"Molecular analysis and nucleotide sequence of the adh1 gene encoding an NADPH-dependent butanol dehydrogenase in the Gram-positive anaerobe Clostridium acetobutylicum." Youngleson J.S., Jones W.A., Jones D.T., Woods D.R. Gene 78:355-364(1989) [PubMed: 2673928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: P262.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M26941 Genomic DNA. Translation: AAA83520.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR001670. ADH_Fe. IPR018211. ADH_Fe_CS. [Graphical view]
Pfam	PF00465. Fe-ADH. 1 hit. [Graphical view]
PROSITE	PS00913. ADH_IRON_1. 1 hit. PS00060. ADH_IRON_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ADH1_CLOSA

Accession

Primary (citable) accession number: P13604

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	June 16, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)