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Protein

Aldehyde dehydrogenase, cytosolic 1

Gene

Aldh1a7

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Can oxidize benzaldehyde, propionaldehyde and acetaldehyde. No detectable activity with retinal.1 Publication

Catalytic activityi

An aldehyde + NAD+ + H2O = a carboxylate + NADH.

Kineticsi

The highest catalytic efficiency is observed with benzaldehyde as substrate. No activity with retinal.

  1. KM=2.4 mM for acetaldehyde1 Publication
  2. KM=1.6 mM for propionaldehyde1 Publication
  3. KM=4.7 µM for benzaldehyde1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei170 – 1701Transition state stabilizerBy similarity
Active sitei269 – 2691Proton acceptorPROSITE-ProRule annotation
Active sitei303 – 3031NucleophilePROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi246 – 2516NADBy similarity

GO - Molecular functioni

  1. 3-chloroallyl aldehyde dehydrogenase activity Source: RGD
  2. aldehyde dehydrogenase (NAD) activity Source: RGD
  3. benzaldehyde dehydrogenase (NAD+) activity Source: RGD
  4. identical protein binding Source: RGD
  5. retinal dehydrogenase activity Source: GO_Central

GO - Biological processi

  1. ethanol catabolic process Source: UniProtKB-UniPathway
  2. operant conditioning Source: RGD
  3. protein homotetramerization Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

SABIO-RKP13601.
UniPathwayiUPA00780; UER00768.

Names & Taxonomyi

Protein namesi
Recommended name:
Aldehyde dehydrogenase, cytosolic 1 (EC:1.2.1.3)
Alternative name(s):
ALDH class 1
ALDH-E1
ALHDII
Aldehyde dehydrogenase family 1 member A7
Aldehyde dehydrogenase phenobarbital-inducible
Gene namesi
Name:Aldh1a7
Synonyms:Aldh-pb, Aldh1, Aldh1a4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi620252. Aldh1a7.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 501500Aldehyde dehydrogenase, cytosolic 1PRO_0000056426Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei91 – 911N6-acetyllysineBy similarity
Modified residuei128 – 1281N6-acetyllysineBy similarity
Modified residuei252 – 2521N6-acetyllysineBy similarity
Modified residuei353 – 3531N6-acetyllysineBy similarity
Modified residuei367 – 3671N6-acetyllysineBy similarity
Modified residuei410 – 4101N6-acetyllysineBy similarity
Modified residuei413 – 4131PhosphoserineBy similarity
Modified residuei419 – 4191N6-acetyllysineBy similarity
Modified residuei435 – 4351N6-acetyllysineBy similarity
Modified residuei495 – 4951N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiP13601.

Expressioni

Tissue specificityi

Very low levels in lung and liver.1 Publication

Inductioni

By phenobarbital.

Gene expression databases

GenevestigatoriP13601.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP13601.
SMRiP13601. Positions 9-501.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldehyde dehydrogenase family.Curated

Phylogenomic databases

HOVERGENiHBG000097.
InParanoidiP13601.
PhylomeDBiP13601.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13601-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSPAQPAVP APLANLKIQH TKIFINNEWH NSLNGKKFPV INPATEEVIC
60 70 80 90 100
HVEEGDKADV DKAVKAARQA FQIGSPWRTM DASERGCLLN KLADLMERDR
110 120 130 140 150
VLLATMESMN AGKIFTHAYL LDTEVSIKAL KYFAGWADKI HGQTIPSDGD
160 170 180 190 200
VFTYTRREPI GVCGQIIPWN GPLILFIWKI GAALSCGNTV IVKPAEQTPL
210 220 230 240 250
TALYMASLIK EAGFPPGVVN VVPGYGSTAG AAISSHMDID KVSFTGSTEV
260 270 280 290 300
GKLIKEAAGK SNLKRVTLEL GGKSPCIVFA DADLDSAVEF AHQGVFFHQG
310 320 330 340 350
QICVAASRLF VEESIYDEFV RRSVERAKKY VLGNPLDSGI SQGPQIDKEQ
360 370 380 390 400
HAKILDLIES GKKEGAKLEC GGGRWGNKGF FVQPTVFSNV TDEMRIAKEE
410 420 430 440 450
IFGPVQQIMK FKSIDEVIKR ANNTPYGLAA GVFTKDLDRA ITVSSALQAG
460 470 480 490 500
TVWVNCYLTL SVQCPFGGFK MSGNGREMGE QGVYEYTELK TVAMKISQKN

S
Length:501
Mass (Da):54,560
Last modified:January 23, 2007 - v2
Checksum:iE6806A1AF736AF1F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23995 mRNA. Translation: AAA40718.1.
PIRiA32616.
RefSeqiNP_058968.14. NM_017272.15.
UniGeneiRn.74044.

Genome annotation databases

UCSCiRGD:620252. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23995 mRNA. Translation: AAA40718.1.
PIRiA32616.
RefSeqiNP_058968.14. NM_017272.15.
UniGeneiRn.74044.

3D structure databases

ProteinModelPortaliP13601.
SMRiP13601. Positions 9-501.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

BindingDBiP13601.
ChEMBLiCHEMBL5354.

Proteomic databases

PRIDEiP13601.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:620252. rat.

Organism-specific databases

RGDi620252. Aldh1a7.

Phylogenomic databases

HOVERGENiHBG000097.
InParanoidiP13601.
PhylomeDBiP13601.

Enzyme and pathway databases

UniPathwayiUPA00780; UER00768.
SABIO-RKP13601.

Gene expression databases

GenevestigatoriP13601.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Phenobarbital-inducible aldehyde dehydrogenase in the rat. cDNA sequence and regulation of the mRNA by phenobarbital in responsive rats."
    Dunn T.J., Koleske A.J., Lindahl R., Pitot H.C.
    J. Biol. Chem. 264:13057-13065(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Rat liver constitutive and phenobarbital-inducible cytosolic aldehyde dehydrogenases are highly homologous proteins that function as distinct isozymes."
    Kathmann E.C., Naylor S., Lipsky J.J.
    Biochemistry 39:11170-11176(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, TISSUE SPECIFICITY.

Entry informationi

Entry nameiAL1A7_RAT
AccessioniPrimary (citable) accession number: P13601
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 23, 2007
Last modified: March 4, 2015
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.