P13597 (ICAM1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Intercellular adhesion molecule 1
Short name=ICAM-1

Alternative name(s):
MALA-2
MyD10
CD_antigen=CD54

Gene names

Name:	Icam1
Synonyms:	Icam-1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	537 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation By similarity.
Subunit structure	Homodimer. Interacts with MUC1 and promotes cell aggregation in epithelial cells. Interacts with ARHGEF26/SGEF By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Expressed at low level on a subpopulation of lymphocytes, macrophages, and endothelial cells, but is strongly induced on these cells, and on fibroblasts and epithelial cells.
Post-translational modification	Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis By similarity.
Sequence similarities	Belongs to the immunoglobulin superfamily. ICAM family. Contains 5 Ig-like C2-type (immunoglobulin-like) domains.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Immunoglobulin domain Repeat Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein Ubl conjugation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell Inferred from electronic annotation. Source: Compara T cell antigen processing and presentation Inferred from direct assay Ref.3. Source: MGI adhesion to symbiont Inferred from electronic annotation. Source: Compara cell adhesion mediated by integrin Inferred from direct assay PubMed 10601245. Source: MGI cell aging Inferred from electronic annotation. Source: Compara cellular response to alkaloid Inferred from electronic annotation. Source: Compara cellular response to glucose stimulus Inferred from direct assay PubMed 17670746. Source: MGI cellular response to hypoxia Inferred from electronic annotation. Source: Compara cellular response to interleukin-1 Inferred from electronic annotation. Source: Compara cellular response to lipopolysaccharide Inferred from electronic annotation. Source: Compara cellular response to nutrient levels Inferred from electronic annotation. Source: Compara cellular response to tumor necrosis factor Inferred from electronic annotation. Source: Compara leukocyte cell-cell adhesion Inferred from mutant phenotype PubMed 8562500. Source: MGI leukocyte migration Inferred from electronic annotation. Source: Compara membrane to membrane docking Inferred from electronic annotation. Source: Compara negative regulation of calcium ion transport Inferred from electronic annotation. Source: Compara ovarian follicle development Inferred from electronic annotation. Source: Compara positive regulation of NF-kappaB transcription factor activity Inferred from electronic annotation. Source: Compara positive regulation of cellular extravasation Inferred from electronic annotation. Source: Compara positive regulation of nitric oxide biosynthetic process Inferred from electronic annotation. Source: Compara positive regulation of vasoconstriction Inferred from electronic annotation. Source: Compara regulation of cell adhesion Inferred from direct assay PubMed 15249579. Source: MGI regulation of cell shape Inferred from electronic annotation. Source: Compara response to amino acid stimulus Inferred from electronic annotation. Source: Compara response to amphetamine Inferred from electronic annotation. Source: Compara response to copper ion Inferred from electronic annotation. Source: Compara response to drug Inferred from electronic annotation. Source: Compara response to ethanol Inferred from electronic annotation. Source: Compara response to gonadotropin stimulus Inferred from electronic annotation. Source: Compara response to ionizing radiation Inferred from electronic annotation. Source: Compara response to organic cyclic compound Inferred from electronic annotation. Source: Compara response to sulfur dioxide Inferred from electronic annotation. Source: Compara virion attachment, binding of host cell surface receptor Inferred from electronic annotation. Source: Compara
Cellular_component	external side of plasma membrane Inferred from direct assay PubMed 16973387. Source: MGI extracellular space Inferred from electronic annotation. Source: Compara immunological synapse Inferred from direct assay PubMed 14973438. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cell surface binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P13597-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P13597-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-34: MASTRAKPTLPLLLALVTVVIPGPGDAQVSIHPR → MITHRHPVREKSINSYQFIKEKQFPAEN

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	By similarity
	Chain	28 – 537	510	Intercellular adhesion molecule 1		PRO_0000014785
Regions
	Topological domain	28 – 485	458	Extracellular Potential
	Transmembrane	486 – 509	24	Helical; Potential
	Topological domain	510 – 537	28	Cytoplasmic Potential
	Domain	41 – 102	62	Ig-like C2-type 1
	Domain	127 – 195	69	Ig-like C2-type 2
	Domain	232 – 299	68	Ig-like C2-type 3
	Domain	327 – 381	55	Ig-like C2-type 4
	Domain	415 – 468	54	Ig-like C2-type 5
	Motif	151 – 153	3	Cell attachment site; atypical Potential
	Motif	179 – 181	3	Cell attachment site Potential
Sites
	Site	469	1	Not glycosylated
	Site	485	1	Not glycosylated
Amino acid modifications
	Glycosylation	47	1	N-linked (GlcNAc...) Ref.7
	Glycosylation	185	1	N-linked (GlcNAc...) Ref.7 Ref.8
	Glycosylation	204	1	N-linked (GlcNAc...) Ref.7 Ref.8
	Glycosylation	267	1	N-linked (GlcNAc...) Ref.7
	Glycosylation	311	1	N-linked (GlcNAc...) Ref.7
	Glycosylation	362	1	N-linked (GlcNAc...) Ref.7 Ref.8
	Glycosylation	388	1	N-linked (GlcNAc...) Ref.7 Ref.8
	Glycosylation	409	1	N-linked (GlcNAc...) Ref.7 Ref.8
	Glycosylation	456	1	N-linked (GlcNAc...) Ref.7
	Glycosylation	469	1	N-linked (GlcNAc...) Ref.7 Ref.8
	Disulfide bond	48 ↔ 91		By similarity
	Disulfide bond	52 ↔ 95		By similarity
	Disulfide bond	134 ↔ 188		By similarity
	Disulfide bond	239 ↔ 292		By similarity
	Disulfide bond	334 ↔ 374		By similarity
	Disulfide bond	422 ↔ 461		By similarity
Natural variations
	Alternative sequence	1 – 34	34	MASTR…SIHPR → MITHRHPVREKSINSYQFIK EKQFPAEN in isoform 2.		VSP_002518
Experimental info
	Sequence conflict	243	1	G → A in AAA37876. Ref.3
	Sequence conflict	370	1	R → A in CAA34621. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: 6124167753774555	FASTA	537	58,844
	10 20 30 40 50 60 MASTRAKPTL PLLLALVTVV IPGPGDAQVS IHPREAFLPQ GGSVQVNCSS SCKEDLSLGL 70 80 90 100 110 120 ETQWLKDELE SGPNWKLFEL SEIGEDSSPL CFENCGTVQS SASATITVYS FPESVELRPL 130 140 150 160 170 180 PAWQQVGKDL TLRCHVDGGA PRTQLSAVLL RGEEILSRQP VGGHPKDPKE ITFTVLASRG 190 200 210 220 230 240 DHGANFSCRT ELDLRPQGLA LFSNVSEARS LRTFDLPATI PKLDTPDLLE VGTQQKLFCS 250 260 270 280 290 300 LEGLFPASEA RIYLELGGQM PTQESTNSSD SVSATALVEV TEEFDRTLPL RCVLELADQI 310 320 330 340 350 360 LETQRTLTVY NFSAPVLTLS QLEVSEGSQV TVKCEAHSGS KVVLLSGVEP RPPTPQVQFT 370 380 390 400 410 420 LNASSEDHKR SFFCSAALEV AGKFLFKNQT LELHVLYGPR LDETDCLGNW TWQEGSQQTL 430 440 450 460 470 480 KCQAWGNPSP KMTCRRKADG ALLPIGVVKS VKQEMNGTYV CHAFSSHGNV TRNVYLTVLY 490 500 510 520 530 HSQNNWTIII LVPVLLVIVG LVMAASYVYN RQRKIRIYKL QKAQEEAIKL KGQAPPP
	Isoform 2 [UniParc]. Checksum: 23B6CCDA9313761F	FASTA	531	58,753
	10 20 30 40 50 60 MITHRHPVRE KSINSYQFIK EKQFPAENEA FLPQGGSVQV NCSSSCKEDL SLGLETQWLK 70 80 90 100 110 120 DELESGPNWK LFELSEIGED SSPLCFENCG TVQSSASATI TVYSFPESVE LRPLPAWQQV 130 140 150 160 170 180 GKDLTLRCHV DGGAPRTQLS AVLLRGEEIL SRQPVGGHPK DPKEITFTVL ASRGDHGANF 190 200 210 220 230 240 SCRTELDLRP QGLALFSNVS EARSLRTFDL PATIPKLDTP DLLEVGTQQK LFCSLEGLFP 250 260 270 280 290 300 ASEARIYLEL GGQMPTQEST NSSDSVSATA LVEVTEEFDR TLPLRCVLEL ADQILETQRT 310 320 330 340 350 360 LTVYNFSAPV LTLSQLEVSE GSQVTVKCEA HSGSKVVLLS GVEPRPPTPQ VQFTLNASSE 370 380 390 400 410 420 DHKRSFFCSA ALEVAGKFLF KNQTLELHVL YGPRLDETDC LGNWTWQEGS QQTLKCQAWG 430 440 450 460 470 480 NPSPKMTCRR KADGALLPIG VVKSVKQEMN GTYVCHAFSS HGNVTRNVYL TVLYHSQNNW 490 500 510 520 530 TIIILVPVLL VIVGLVMAAS YVYNRQRKIR IYKLQKAQEE AIKLKGQAPP P

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of murine intercellular adhesion molecule (ICAM-1)." Horley K.J., Carpenito C., Baker B., Takei F. EMBO J. 8:2889-2896(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: BALB/c. Tissue: Myeloma.
[2]	Takei F. Submitted (APR-1990) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"Isolation of the murine intercellular adhesion molecule 1 (ICAM-1) gene. ICAM-1 enhances antigen-specific T cell activation." Siu G., Hedrick S.M., Brian A.A. J. Immunol. 143:3813-3820(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Nucleotide sequence of the cDNA for murine intercellular adhesion molecule-1 (ICAM-1)." Ballantyne C.M., O'Brien W.E., Beaudet A.L. Nucleic Acids Res. 17:5853-5853(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6 X CBA. Tissue: Thymus.
[5]	"Characterization of the murine Icam-1 gene." Ballantyne C.M., Sligh J.E., Dai X.Y., Beaudet A.L. Genomics 14:1076-1080(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NIH Swiss.
[6]	"Complexity of the immediate early response of myeloid cells to terminal differentiation and growth arrest includes ICAM-1, Jun-B and histone variants." Lord K.A., Hoffman-Liebermann B., Liebermann D.A. Oncogene 5:387-396(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-131.
[7]	"N-glycan structures and N-glycosylation sites of mouse soluble intercellular adhesion molecule-1 revealed by MALDI-TOF and FTICR mass spectrometry." Otto V.I., Damoc E., Cueni L.N., Schurpf T., Frei R., Ali S., Callewaert N., Moise A., Leary J.A., Folkers G., Przybylski M. Glycobiology 16:1033-1044(2006) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION AT ASN-47; ASN-185; ASN-204; ASN-267; ASN-311; ASN-362; ASN-388; ASN-409 AND ASN-456, ABSENCE OF GLYCOSYLATION AT ASN-469 AND ASN-485, MASS SPECTROMETRY.
[8]	"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins." Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D. Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-185; ASN-204; ASN-362; ASN-388; ASN-409 AND ASN-469, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - Glycan Binding

ICAM-1

Cross-references

Sequence databases
EMBL GenBank DDBJ	X16624 mRNA. Translation: CAA34621.1. X16625 mRNA. Translation: CAA34622.1. M31585 mRNA. Translation: AAA37876.1. X52264 mRNA. Translation: CAA36507.1. M90551 , M90546, M90547, M90548, M90549, M90550 Genomic DNA. Translation: AAA37875.1. X54331 mRNA. No translation available.
IPI	IPI00122973. IPI00230702.
PIR	A45815. I49769. S06016.
RefSeq	NP_034623.1. NM_010493.2.
UniGene	Mm.435508.
3D structure databases
ProteinModelPortal	P13597.
SMR	P13597. Positions 28-479.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-29096N.
PTM databases
PhosphoSite	P13597.
Proteomic databases
PaxDb	P13597.
PRIDE	P13597.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000086399; ENSMUSP00000083587; ENSMUSG00000037405.
GeneID	15894.
KEGG	mmu:15894.
Organism-specific databases
CTD	3383.
MGI	MGI:96392. Icam1.
Phylogenomic databases
eggNOG	NOG146347.
HOGENOM	HOG000059554.
HOVERGEN	HBG052074.
InParanoid	P13597.
KO	K06490.
OMA	RDCPGNW.
OrthoDB	EOG4THVT8.
Gene expression databases
ArrayExpress	P13597.
Bgee	P13597.
CleanEx	MM_ICAM1.
Genevestigator	P13597.
GermOnline	ENSMUSG00000037405. Mus musculus.
Family and domain databases
Gene3D	2.60.40.10. 5 hits.
InterPro	IPR003988. ICAM. IPR013768. ICAM_N. IPR003987. ICAM_VCAM_N. IPR007110. Ig-like_dom. IPR013783. Ig-like_fold. IPR003599. Ig_sub. [Graphical view]
Pfam	PF03921. ICAM_N. 1 hit. [Graphical view]
PRINTS	PR01473. ICAM. PR01472. ICAMVCAM1.
SMART	SM00409. IG. 2 hits. [Graphical view]
PROSITE	PS50835. IG_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	288604.
SOURCE	Search...

Entry information

Entry name

ICAM1_MOUSE

Accession

Primary (citable) accession number: P13597
Secondary accession number(s): Q61828

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	May 1, 2013
This is version 141 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program