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Protein

Neural cell adhesion molecule 1

Gene

Ncam1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.

GO - Molecular functioni

  • cytoskeletal protein binding Source: RGD
  • fibroblast growth factor receptor binding Source: RGD
  • heparin binding Source: UniProtKB-KW
  • LRR domain binding Source: RGD
  • phosphatase binding Source: RGD

GO - Biological processi

  • aging Source: RGD
  • animal organ regeneration Source: RGD
  • axon guidance Source: Reactome
  • calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules Source: RGD
  • cell adhesion Source: RGD
  • cellular response to inorganic substance Source: RGD
  • cellular response to molecule of bacterial origin Source: RGD
  • learning or memory Source: RGD
  • multicellular organismal response to stress Source: RGD
  • negative regulation of cell death Source: RGD
  • neuron development Source: RGD
  • peripheral nervous system axon regeneration Source: RGD
  • positive regulation of cardiac muscle cell proliferation Source: RGD
  • regulation of sensory perception of pain Source: RGD
  • regulation of synaptic plasticity Source: InterPro
  • response to cocaine Source: RGD
  • response to drug Source: RGD
  • response to inorganic substance Source: RGD
  • response to lead ion Source: RGD
  • thalamus development Source: RGD
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Heparin-binding

Enzyme and pathway databases

ReactomeiR-RNO-375165. NCAM signaling for neurite out-growth.
R-RNO-419037. NCAM1 interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Neural cell adhesion molecule 1
Short name:
N-CAM-1
Short name:
NCAM-1
Alternative name(s):
CD_antigen: CD56
Gene namesi
Name:Ncam1
Synonyms:Ncam
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi67378. Ncam1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini20 – 721ExtracellularSequence analysisAdd BLAST702
Transmembranei722 – 739HelicalSequence analysisAdd BLAST18
Topological domaini740 – 858CytoplasmicSequence analysisAdd BLAST119

GO - Cellular componenti

  • growth cone Source: RGD
  • integral component of membrane Source: UniProtKB-KW
  • neuronal cell body Source: RGD
  • plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19By similarityAdd BLAST19
ChainiPRO_000001501520 – 858Neural cell adhesion molecule 1Add BLAST839

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi41 ↔ 96PROSITE-ProRule annotation
Disulfide bondi139 ↔ 189PROSITE-ProRule annotation
Glycosylationi222N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi235 ↔ 288PROSITE-ProRule annotation
Glycosylationi316N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi330 ↔ 396PROSITE-ProRule annotation
Glycosylationi348N-linked (GlcNAc...)Sequence analysis1
Glycosylationi434N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi437 ↔ 490PROSITE-ProRule annotation
Glycosylationi460N-linked (GlcNAc...)Sequence analysis1
Glycosylationi489N-linked (GlcNAc...)Sequence analysis1
Modified residuei780PhosphoserineCombined sources1
Modified residuei784PhosphoserineCombined sources1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP13596.
PRIDEiP13596.

PTM databases

iPTMnetiP13596.
PhosphoSitePlusiP13596.
SwissPalmiP13596.
UniCarbKBiP13596.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Fgfr1P160922EBI-916499,EBI-7953898From a different organism.
Fgfr2Q632372EBI-916499,EBI-8523405

GO - Molecular functioni

  • cytoskeletal protein binding Source: RGD
  • fibroblast growth factor receptor binding Source: RGD
  • LRR domain binding Source: RGD
  • phosphatase binding Source: RGD

Protein-protein interaction databases

BioGridi246729. 3 interactors.
IntActiP13596. 3 interactors.
MINTiMINT-2635713.
STRINGi10116.ENSRNOP00000048442.

Structurei

Secondary structure

1858
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi22 – 32Combined sources11
Beta strandi37 – 43Combined sources7
Beta strandi51 – 55Combined sources5
Beta strandi65 – 75Combined sources11
Beta strandi78 – 83Combined sources6
Helixi88 – 90Combined sources3
Beta strandi92 – 99Combined sources8
Beta strandi105 – 115Combined sources11
Beta strandi118 – 122Combined sources5
Beta strandi125 – 128Combined sources4
Beta strandi135 – 137Combined sources3
Beta strandi140 – 142Combined sources3
Beta strandi148 – 153Combined sources6
Helixi158 – 161Combined sources4
Beta strandi166 – 168Combined sources3
Beta strandi174 – 176Combined sources3
Helixi181 – 183Combined sources3
Beta strandi185 – 193Combined sources9
Helixi194 – 196Combined sources3
Beta strandi198 – 207Combined sources10
Beta strandi219 – 224Combined sources6
Beta strandi231 – 241Combined sources11
Beta strandi244 – 249Combined sources6
Beta strandi262 – 266Combined sources5
Beta strandi272 – 275Combined sources4
Helixi280 – 282Combined sources3
Beta strandi284 – 292Combined sources9
Beta strandi295 – 306Combined sources12
Beta strandi616 – 622Combined sources7
Turni623 – 626Combined sources4
Beta strandi627 – 633Combined sources7
Beta strandi637 – 639Combined sources3
Beta strandi642 – 654Combined sources13
Beta strandi667 – 673Combined sources7
Beta strandi679 – 688Combined sources10
Beta strandi691 – 701Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EPFX-ray1.85A/B/C/D20-208[»]
1LWRNMR-A612-705[»]
1QZ1X-ray2.00A20-308[»]
ProteinModelPortaliP13596.
SMRiP13596.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP13596.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 111Ig-like C2-type 1Add BLAST92
Domaini116 – 205Ig-like C2-type 2Add BLAST90
Domaini212 – 302Ig-like C2-type 3Add BLAST91
Domaini309 – 414Ig-like C2-type 4Add BLAST106
Domaini417 – 502Ig-like C2-type 5Add BLAST86
Domaini510 – 609Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST100
Domaini611 – 706Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST96

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni152 – 156Heparin-bindingSequence analysis5
Regioni161 – 165Heparin-bindingSequence analysis5

Sequence similaritiesi

Contains 2 fibronectin type-III domains.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IQJI. Eukaryota.
ENOG410XTGB. LUCA.
HOGENOMiHOG000074124.
HOVERGENiHBG052579.
InParanoidiP13596.
KOiK06491.
PhylomeDBiP13596.

Family and domain databases

CDDicd00063. FN3. 2 hits.
Gene3Di2.60.40.10. 7 hits.
InterProiIPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR033019. Ncam1.
IPR009138. Neural_cell_adh.
[Graphical view]
PANTHERiPTHR10489:SF34. PTHR10489:SF34. 2 hits.
PfamiPF00041. fn3. 2 hits.
PF07679. I-set. 3 hits.
[Graphical view]
PRINTSiPR01838. NCAMFAMILY.
SMARTiSM00060. FN3. 2 hits.
SM00409. IG. 5 hits.
SM00408. IGc2. 5 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 5 hits.
SSF49265. SSF49265. 1 hit.
PROSITEiPS50853. FN3. 2 hits.
PS50835. IG_LIKE. 5 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced.
Isoform 1 (identifier: P13596-1) [UniParc]FASTAAdd to basket
Also known as: N-CAM 140

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLRTKDLIWT LFFLGTAVSL QVDIVPSQGE ISVGESKFFL CQVAGDAKDK
60 70 80 90 100
DISWFSPNGE KLSPNQQRIS VVWNDDDSST LTIYNANIDD AGIYKCVVTA
110 120 130 140 150
EDGTQSEATV NVKIFQKLMF KNAPTPQEFK EGEDAVIVCD VVSSLPPTII
160 170 180 190 200
WKHKGRDVIL KKDVRFIVLS NNYLQIRGIK KTDEGTYRCE GRILARGEIN
210 220 230 240 250
FKDIQVIVNV PPTVQARQSI VNATANLGQS VTLVCDADGF PEPTMSWTKD
260 270 280 290 300
GEPIENEEED DEKHIFSDDS SELTIRNVDK NDEAEYVCIA ENKAGEQDAS
310 320 330 340 350
IHLKVFAKPK ITYVENQTAM ELEEQVTLTC EASGDPIPSI TWRTSTRNIS
360 370 380 390 400
SEEKASWTRP EKQETLDGHM VVRSHARVSS LTLKSIQYTD AGEYICTASN
410 420 430 440 450
TIGQDSQSMY LEVQYAPKLQ GPVAVYTWEG NQVNITCEVF AYPSATISWF
460 470 480 490 500
RDGQLLPSSN YSNIKIYNTP SASYLEVTPD SENDFGNYNC TAVNRIGQES
510 520 530 540 550
LEFILVQADT PSSPSIDRVE PYSSTAQVQF DEPEATGGVP ILKYKAEWKS
560 570 580 590 600
LGEEAWHSKW YDAKEANMEG IVTIMGLKPE TRYAVRLAAL NGKGLGEISA
610 620 630 640 650
ATEFKTQPVR EPSAPKLEGQ MGEDGNSIKV NLIKQDDGGS PIRHYLVKYR
660 670 680 690 700
ALASEWKPEI RLPSGSDHVM LKSLDWNAEY EVYVVAENQQ GKSKAAHFVF
710 720 730 740 750
RTSAQPTAIP ANGSPTAGLS TGAIVGILIV IFVLLLVVMD ITCYFLNKCG
760 770 780 790 800
LLMCIAVNLC GKAGPGAKGK DMEEGKAAFS KDESKEPIVE VRTEEERTPN
810 820 830 840 850
HDGGKHTEPN ETTPLTEPEK GPVETKSEPQ ESEAKPAPTE VKTVPNEATQ

TKENESKA
Length:858
Mass (Da):94,658
Last modified:January 1, 1990 - v1
Checksum:iEA1A06A4EA0550F6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06564 mRNA. Translation: CAA29809.1.
M32611 Genomic DNA. Translation: AAA41679.1.
PIRiS00846. IJRTNC.
RefSeqiNP_113709.1. NM_031521.1. [P13596-1]
UniGeneiRn.11283.
Rn.214248.

Genome annotation databases

GeneIDi24586.
KEGGirno:24586.
UCSCiRGD:67378. rat. [P13596-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06564 mRNA. Translation: CAA29809.1.
M32611 Genomic DNA. Translation: AAA41679.1.
PIRiS00846. IJRTNC.
RefSeqiNP_113709.1. NM_031521.1. [P13596-1]
UniGeneiRn.11283.
Rn.214248.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EPFX-ray1.85A/B/C/D20-208[»]
1LWRNMR-A612-705[»]
1QZ1X-ray2.00A20-308[»]
ProteinModelPortaliP13596.
SMRiP13596.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi246729. 3 interactors.
IntActiP13596. 3 interactors.
MINTiMINT-2635713.
STRINGi10116.ENSRNOP00000048442.

PTM databases

iPTMnetiP13596.
PhosphoSitePlusiP13596.
SwissPalmiP13596.
UniCarbKBiP13596.

Proteomic databases

PaxDbiP13596.
PRIDEiP13596.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi24586.
KEGGirno:24586.
UCSCiRGD:67378. rat. [P13596-1]

Organism-specific databases

CTDi4684.
RGDi67378. Ncam1.

Phylogenomic databases

eggNOGiENOG410IQJI. Eukaryota.
ENOG410XTGB. LUCA.
HOGENOMiHOG000074124.
HOVERGENiHBG052579.
InParanoidiP13596.
KOiK06491.
PhylomeDBiP13596.

Enzyme and pathway databases

ReactomeiR-RNO-375165. NCAM signaling for neurite out-growth.
R-RNO-419037. NCAM1 interactions.

Miscellaneous databases

EvolutionaryTraceiP13596.
PROiP13596.

Family and domain databases

CDDicd00063. FN3. 2 hits.
Gene3Di2.60.40.10. 7 hits.
InterProiIPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR033019. Ncam1.
IPR009138. Neural_cell_adh.
[Graphical view]
PANTHERiPTHR10489:SF34. PTHR10489:SF34. 2 hits.
PfamiPF00041. fn3. 2 hits.
PF07679. I-set. 3 hits.
[Graphical view]
PRINTSiPR01838. NCAMFAMILY.
SMARTiSM00060. FN3. 2 hits.
SM00409. IG. 5 hits.
SM00408. IGc2. 5 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 5 hits.
SSF49265. SSF49265. 1 hit.
PROSITEiPS50853. FN3. 2 hits.
PS50835. IG_LIKE. 5 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNCAM1_RAT
AccessioniPrimary (citable) accession number: P13596
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: November 30, 2016
This is version 158 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.