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P13596 (NCAM1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 135. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Neural cell adhesion molecule 1

Short name=N-CAM-1
Short name=NCAM-1
Alternative name(s):
CD_antigen=CD56
Gene names
Name:Ncam1
Synonyms:Ncam
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length858 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.

Subcellular location

Cell membrane; Single-pass type I membrane protein.

Sequence similarities

Contains 2 fibronectin type-III domains.

Contains 5 Ig-like C2-type (immunoglobulin-like) domains.

Ontologies

Keywords
   Biological processCell adhesion
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
   DomainImmunoglobulin domain
Repeat
Signal
Transmembrane
Transmembrane helix
   LigandHeparin-binding
   PTMDisulfide bond
Glycoprotein
Phosphoprotein
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processaging

Inferred from direct assay PubMed 17904697. Source: RGD

calcium-independent cell-cell adhesion

Traceable author statement PubMed 15865439. Source: RGD

cell adhesion

Traceable author statement Ref.1. Source: RGD

cellular response to inorganic substance

Inferred from expression pattern PubMed 17537562. Source: RGD

cellular response to molecule of bacterial origin

Inferred from expression pattern PubMed 19853610. Source: RGD

learning or memory

Inferred from direct assay PubMed 18702715. Source: RGD

multicellular organismal response to stress

Inferred from direct assay PubMed 17429411. Source: RGD

negative regulation of cell death

Inferred from mutant phenotype PubMed 18757519. Source: RGD

neuron development

Inferred from direct assay PubMed 16958105. Source: RGD

organ regeneration

Inferred from expression pattern PubMed 16609270. Source: RGD

peripheral nervous system axon regeneration

Inferred from expression pattern PubMed 15679872. Source: RGD

positive regulation of cardiac muscle cell proliferation

Inferred from mutant phenotype PubMed 19853610. Source: RGD

regulation of sensory perception of pain

Inferred from mutant phenotype PubMed 17967506. Source: RGD

response to cocaine

Inferred from direct assay PubMed 16081054. Source: RGD

response to fluoxetine

Inferred from direct assay PubMed 17307340. Source: RGD

response to inorganic substance

Inferred from expression pattern PubMed 20412599. Source: RGD

response to lead ion

Inferred from direct assay PubMed 18499259. Source: RGD

thalamus development

Inferred from direct assay PubMed 17292800. Source: RGD

   Cellular_componentgrowth cone

Traceable author statement PubMed 15865439. Source: RGD

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

neuronal cell body

Inferred from direct assay PubMed 16216431. Source: RGD

plasma membrane

Traceable author statement Ref.1. Source: RGD

   Molecular_functionLRR domain binding

Inferred from physical interaction PubMed 15865439. Source: RGD

cytoskeletal protein binding

Traceable author statement PubMed 15865439. Source: RGD

fibroblast growth factor receptor binding

Traceable author statement PubMed 15865439. Source: RGD

heparin binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphatase binding

Inferred from direct assay PubMed 15865439. Source: RGD

protein binding

Inferred from physical interaction PubMed 16709412. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Fgfr1P160922EBI-916499,EBI-7953898From a different organism.
Fgfr2Q632372EBI-916499,EBI-8523405

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced.
Isoform 1 (identifier: P13596-1)

Also known as: N-CAM 140;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 By similarity
Chain20 – 858839Neural cell adhesion molecule 1
PRO_0000015015

Regions

Topological domain20 – 721702Extracellular Potential
Transmembrane722 – 73918Helical; Potential
Topological domain740 – 858119Cytoplasmic Potential
Domain20 – 11192Ig-like C2-type 1
Domain116 – 20590Ig-like C2-type 2
Domain212 – 30291Ig-like C2-type 3
Domain309 – 414106Ig-like C2-type 4
Domain417 – 50286Ig-like C2-type 5
Domain510 – 609100Fibronectin type-III 1
Domain611 – 70696Fibronectin type-III 2
Region152 – 1565Heparin-binding Potential
Region161 – 1655Heparin-binding Potential

Amino acid modifications

Modified residue7841Phosphoserine By similarity
Glycosylation2221N-linked (GlcNAc...) Potential
Glycosylation3161N-linked (GlcNAc...) Potential
Glycosylation3481N-linked (GlcNAc...) Potential
Glycosylation4341N-linked (GlcNAc...) Potential
Glycosylation4601N-linked (GlcNAc...) Potential
Glycosylation4891N-linked (GlcNAc...) Potential
Disulfide bond41 ↔ 96 By similarity
Disulfide bond139 ↔ 189 By similarity
Disulfide bond235 ↔ 288 By similarity
Disulfide bond330 ↔ 396 By similarity
Disulfide bond437 ↔ 490 By similarity

Secondary structure

...................................................................... 858
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (N-CAM 140) [UniParc].

Last modified January 1, 1990. Version 1.
Checksum: EA1A06A4EA0550F6

FASTA85894,658
        10         20         30         40         50         60 
MLRTKDLIWT LFFLGTAVSL QVDIVPSQGE ISVGESKFFL CQVAGDAKDK DISWFSPNGE 

        70         80         90        100        110        120 
KLSPNQQRIS VVWNDDDSST LTIYNANIDD AGIYKCVVTA EDGTQSEATV NVKIFQKLMF 

       130        140        150        160        170        180 
KNAPTPQEFK EGEDAVIVCD VVSSLPPTII WKHKGRDVIL KKDVRFIVLS NNYLQIRGIK 

       190        200        210        220        230        240 
KTDEGTYRCE GRILARGEIN FKDIQVIVNV PPTVQARQSI VNATANLGQS VTLVCDADGF 

       250        260        270        280        290        300 
PEPTMSWTKD GEPIENEEED DEKHIFSDDS SELTIRNVDK NDEAEYVCIA ENKAGEQDAS 

       310        320        330        340        350        360 
IHLKVFAKPK ITYVENQTAM ELEEQVTLTC EASGDPIPSI TWRTSTRNIS SEEKASWTRP 

       370        380        390        400        410        420 
EKQETLDGHM VVRSHARVSS LTLKSIQYTD AGEYICTASN TIGQDSQSMY LEVQYAPKLQ 

       430        440        450        460        470        480 
GPVAVYTWEG NQVNITCEVF AYPSATISWF RDGQLLPSSN YSNIKIYNTP SASYLEVTPD 

       490        500        510        520        530        540 
SENDFGNYNC TAVNRIGQES LEFILVQADT PSSPSIDRVE PYSSTAQVQF DEPEATGGVP 

       550        560        570        580        590        600 
ILKYKAEWKS LGEEAWHSKW YDAKEANMEG IVTIMGLKPE TRYAVRLAAL NGKGLGEISA 

       610        620        630        640        650        660 
ATEFKTQPVR EPSAPKLEGQ MGEDGNSIKV NLIKQDDGGS PIRHYLVKYR ALASEWKPEI 

       670        680        690        700        710        720 
RLPSGSDHVM LKSLDWNAEY EVYVVAENQQ GKSKAAHFVF RTSAQPTAIP ANGSPTAGLS 

       730        740        750        760        770        780 
TGAIVGILIV IFVLLLVVMD ITCYFLNKCG LLMCIAVNLC GKAGPGAKGK DMEEGKAAFS 

       790        800        810        820        830        840 
KDESKEPIVE VRTEEERTPN HDGGKHTEPN ETTPLTEPEK GPVETKSEPQ ESEAKPAPTE 

       850 
VKTVPNEATQ TKENESKA 

« Hide

References

[1]"Identification of a cDNA clone that contains the complete coding sequence for a 140-kD rat NCAM polypeptide."
Small S.J., Shull G.E., Santoni M.-J., Akeson R.
J. Cell Biol. 105:2335-2345(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"Expression of the unique NCAM VASE exon is independently regulated in distinct tissues during development."
Small S.J., Akeson R.
J. Cell Biol. 111:2089-2096(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 340-381.
[3]"Polypeptide variation in an N-CAM extracellular immunoglobulin-like fold is developmentally regulated through alternative splicing."
Small S.J., Haines S.L., Akeson R.A.
Neuron 1:1007-1017(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 355-364.
[4]Lubec G., Kang S.U.
Submitted (JUL-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 38-48 AND 594-605, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X06564 mRNA. Translation: CAA29809.1.
M32611 Genomic DNA. Translation: AAA41679.1.
PIRIJRTNC. S00846.
RefSeqNP_113709.1. NM_031521.1. [P13596-1]
UniGeneRn.11283.
Rn.214248.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1EPFX-ray1.85A/B/C/D20-208[»]
1LWRNMR-A612-705[»]
1QZ1X-ray2.00A20-308[»]
ProteinModelPortalP13596.
SMRP13596. Positions 20-308, 507-705.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid246729. 2 interactions.
IntActP13596. 3 interactions.
MINTMINT-2635713.
STRING10116.ENSRNOP00000046828.

PTM databases

PhosphoSiteP13596.

Proteomic databases

PaxDbP13596.
PRIDEP13596.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID24586.
KEGGrno:24586.
UCSCRGD:67378. rat. [P13596-1]

Organism-specific databases

CTD4684.
RGD67378. Ncam1.

Phylogenomic databases

eggNOGNOG308439.
HOGENOMHOG000074124.
HOVERGENHBG052579.
KOK06491.
PhylomeDBP13596.

Enzyme and pathway databases

ReactomeREACT_188576. Developmental Biology.
REACT_195021. Developmental Biology.

Gene expression databases

GenevestigatorP13596.

Family and domain databases

Gene3D2.60.40.10. 7 hits.
InterProIPR003961. Fibronectin_type3.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003598. Ig_sub2.
IPR009138. Neural_cell_adh.
[Graphical view]
PfamPF00041. fn3. 2 hits.
PF07679. I-set. 5 hits.
[Graphical view]
PRINTSPR01838. NCAMFAMILY.
SMARTSM00060. FN3. 2 hits.
SM00408. IGc2. 5 hits.
[Graphical view]
SUPFAMSSF49265. SSF49265. 1 hit.
PROSITEPS50853. FN3. 2 hits.
PS50835. IG_LIKE. 5 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP13596.
NextBio603762.

Entry information

Entry nameNCAM1_RAT
AccessionPrimary (citable) accession number: P13596
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: June 11, 2014
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references