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P13596

- NCAM1_RAT

UniProt

P13596 - NCAM1_RAT

Protein

Neural cell adhesion molecule 1

Gene

Ncam1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 137 (01 Oct 2014)
      Sequence version 1 (01 Jan 1990)
      Previous versions | rss
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    Functioni

    This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.

    GO - Molecular functioni

    1. cytoskeletal protein binding Source: RGD
    2. fibroblast growth factor receptor binding Source: RGD
    3. heparin binding Source: UniProtKB-KW
    4. LRR domain binding Source: RGD
    5. phosphatase binding Source: RGD
    6. protein binding Source: IntAct

    GO - Biological processi

    1. aging Source: RGD
    2. calcium-independent cell-cell adhesion Source: RGD
    3. cell adhesion Source: RGD
    4. cellular response to inorganic substance Source: RGD
    5. cellular response to molecule of bacterial origin Source: RGD
    6. learning or memory Source: RGD
    7. multicellular organismal response to stress Source: RGD
    8. negative regulation of cell death Source: RGD
    9. neuron development Source: RGD
    10. organ regeneration Source: RGD
    11. peripheral nervous system axon regeneration Source: RGD
    12. positive regulation of cardiac muscle cell proliferation Source: RGD
    13. regulation of sensory perception of pain Source: RGD
    14. response to cocaine Source: RGD
    15. response to fluoxetine Source: RGD
    16. response to inorganic substance Source: RGD
    17. response to lead ion Source: RGD
    18. thalamus development Source: RGD

    Keywords - Biological processi

    Cell adhesion

    Keywords - Ligandi

    Heparin-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Neural cell adhesion molecule 1
    Short name:
    N-CAM-1
    Short name:
    NCAM-1
    Alternative name(s):
    CD_antigen: CD56
    Gene namesi
    Name:Ncam1
    Synonyms:Ncam
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi67378. Ncam1.

    Subcellular locationi

    GO - Cellular componenti

    1. growth cone Source: RGD
    2. integral component of membrane Source: UniProtKB-KW
    3. neuronal cell body Source: RGD
    4. plasma membrane Source: RGD

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919By similarityAdd
    BLAST
    Chaini20 – 858839Neural cell adhesion molecule 1PRO_0000015015Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi41 ↔ 96PROSITE-ProRule annotation
    Disulfide bondi139 ↔ 189PROSITE-ProRule annotation
    Glycosylationi222 – 2221N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi235 ↔ 288PROSITE-ProRule annotation
    Glycosylationi316 – 3161N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi330 ↔ 396PROSITE-ProRule annotation
    Glycosylationi348 – 3481N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi434 – 4341N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi437 ↔ 490PROSITE-ProRule annotation
    Glycosylationi460 – 4601N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi489 – 4891N-linked (GlcNAc...)Sequence Analysis
    Modified residuei784 – 7841PhosphoserineBy similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiP13596.
    PRIDEiP13596.

    PTM databases

    PhosphoSiteiP13596.

    Expressioni

    Gene expression databases

    GenevestigatoriP13596.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Fgfr1P160922EBI-916499,EBI-7953898From a different organism.
    Fgfr2Q632372EBI-916499,EBI-8523405

    Protein-protein interaction databases

    BioGridi246729. 2 interactions.
    IntActiP13596. 3 interactions.
    MINTiMINT-2635713.
    STRINGi10116.ENSRNOP00000046828.

    Structurei

    Secondary structure

    1
    858
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi22 – 3211
    Beta strandi37 – 437
    Beta strandi51 – 555
    Beta strandi65 – 7511
    Beta strandi78 – 836
    Helixi88 – 903
    Beta strandi92 – 998
    Beta strandi105 – 11511
    Beta strandi118 – 1225
    Beta strandi125 – 1284
    Beta strandi135 – 1373
    Beta strandi140 – 1423
    Beta strandi148 – 1536
    Helixi158 – 1614
    Beta strandi166 – 1683
    Beta strandi174 – 1763
    Helixi181 – 1833
    Beta strandi185 – 1939
    Helixi194 – 1963
    Beta strandi198 – 20710
    Beta strandi219 – 2246
    Beta strandi231 – 24111
    Beta strandi244 – 2496
    Beta strandi262 – 2665
    Beta strandi272 – 2754
    Helixi280 – 2823
    Beta strandi284 – 2929
    Beta strandi295 – 30612
    Beta strandi616 – 6227
    Turni623 – 6264
    Beta strandi627 – 6337
    Beta strandi637 – 6393
    Beta strandi642 – 65413
    Beta strandi667 – 6737
    Beta strandi679 – 68810
    Beta strandi691 – 70111

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1EPFX-ray1.85A/B/C/D20-208[»]
    1LWRNMR-A612-705[»]
    1QZ1X-ray2.00A20-308[»]
    ProteinModelPortaliP13596.
    SMRiP13596. Positions 20-308, 507-705.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP13596.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini20 – 721702ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini740 – 858119CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei722 – 73918HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini20 – 11192Ig-like C2-type 1Add
    BLAST
    Domaini116 – 20590Ig-like C2-type 2Add
    BLAST
    Domaini212 – 30291Ig-like C2-type 3Add
    BLAST
    Domaini309 – 414106Ig-like C2-type 4Add
    BLAST
    Domaini417 – 50286Ig-like C2-type 5Add
    BLAST
    Domaini510 – 609100Fibronectin type-III 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini611 – 70696Fibronectin type-III 2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni152 – 1565Heparin-bindingSequence Analysis
    Regioni161 – 1655Heparin-bindingSequence Analysis

    Sequence similaritiesi

    Contains 2 fibronectin type-III domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG308439.
    HOGENOMiHOG000074124.
    HOVERGENiHBG052579.
    KOiK06491.
    PhylomeDBiP13596.

    Family and domain databases

    Gene3Di2.60.40.10. 7 hits.
    InterProiIPR003961. Fibronectin_type3.
    IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR013098. Ig_I-set.
    IPR003598. Ig_sub2.
    IPR009138. Neural_cell_adh.
    [Graphical view]
    PfamiPF00041. fn3. 2 hits.
    PF07679. I-set. 5 hits.
    [Graphical view]
    PRINTSiPR01838. NCAMFAMILY.
    SMARTiSM00060. FN3. 2 hits.
    SM00408. IGc2. 5 hits.
    [Graphical view]
    SUPFAMiSSF49265. SSF49265. 1 hit.
    PROSITEiPS50853. FN3. 2 hits.
    PS50835. IG_LIKE. 5 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced.

    Isoform 1 (identifier: P13596-1) [UniParc]FASTAAdd to Basket

    Also known as: N-CAM 140

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MLRTKDLIWT LFFLGTAVSL QVDIVPSQGE ISVGESKFFL CQVAGDAKDK    50
    DISWFSPNGE KLSPNQQRIS VVWNDDDSST LTIYNANIDD AGIYKCVVTA 100
    EDGTQSEATV NVKIFQKLMF KNAPTPQEFK EGEDAVIVCD VVSSLPPTII 150
    WKHKGRDVIL KKDVRFIVLS NNYLQIRGIK KTDEGTYRCE GRILARGEIN 200
    FKDIQVIVNV PPTVQARQSI VNATANLGQS VTLVCDADGF PEPTMSWTKD 250
    GEPIENEEED DEKHIFSDDS SELTIRNVDK NDEAEYVCIA ENKAGEQDAS 300
    IHLKVFAKPK ITYVENQTAM ELEEQVTLTC EASGDPIPSI TWRTSTRNIS 350
    SEEKASWTRP EKQETLDGHM VVRSHARVSS LTLKSIQYTD AGEYICTASN 400
    TIGQDSQSMY LEVQYAPKLQ GPVAVYTWEG NQVNITCEVF AYPSATISWF 450
    RDGQLLPSSN YSNIKIYNTP SASYLEVTPD SENDFGNYNC TAVNRIGQES 500
    LEFILVQADT PSSPSIDRVE PYSSTAQVQF DEPEATGGVP ILKYKAEWKS 550
    LGEEAWHSKW YDAKEANMEG IVTIMGLKPE TRYAVRLAAL NGKGLGEISA 600
    ATEFKTQPVR EPSAPKLEGQ MGEDGNSIKV NLIKQDDGGS PIRHYLVKYR 650
    ALASEWKPEI RLPSGSDHVM LKSLDWNAEY EVYVVAENQQ GKSKAAHFVF 700
    RTSAQPTAIP ANGSPTAGLS TGAIVGILIV IFVLLLVVMD ITCYFLNKCG 750
    LLMCIAVNLC GKAGPGAKGK DMEEGKAAFS KDESKEPIVE VRTEEERTPN 800
    HDGGKHTEPN ETTPLTEPEK GPVETKSEPQ ESEAKPAPTE VKTVPNEATQ 850
    TKENESKA 858
    Length:858
    Mass (Da):94,658
    Last modified:January 1, 1990 - v1
    Checksum:iEA1A06A4EA0550F6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X06564 mRNA. Translation: CAA29809.1.
    M32611 Genomic DNA. Translation: AAA41679.1.
    PIRiS00846. IJRTNC.
    RefSeqiNP_113709.1. NM_031521.1. [P13596-1]
    UniGeneiRn.11283.
    Rn.214248.

    Genome annotation databases

    GeneIDi24586.
    KEGGirno:24586.
    UCSCiRGD:67378. rat. [P13596-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X06564 mRNA. Translation: CAA29809.1 .
    M32611 Genomic DNA. Translation: AAA41679.1 .
    PIRi S00846. IJRTNC.
    RefSeqi NP_113709.1. NM_031521.1. [P13596-1 ]
    UniGenei Rn.11283.
    Rn.214248.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1EPF X-ray 1.85 A/B/C/D 20-208 [» ]
    1LWR NMR - A 612-705 [» ]
    1QZ1 X-ray 2.00 A 20-308 [» ]
    ProteinModelPortali P13596.
    SMRi P13596. Positions 20-308, 507-705.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 246729. 2 interactions.
    IntActi P13596. 3 interactions.
    MINTi MINT-2635713.
    STRINGi 10116.ENSRNOP00000046828.

    PTM databases

    PhosphoSitei P13596.

    Proteomic databases

    PaxDbi P13596.
    PRIDEi P13596.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 24586.
    KEGGi rno:24586.
    UCSCi RGD:67378. rat. [P13596-1 ]

    Organism-specific databases

    CTDi 4684.
    RGDi 67378. Ncam1.

    Phylogenomic databases

    eggNOGi NOG308439.
    HOGENOMi HOG000074124.
    HOVERGENi HBG052579.
    KOi K06491.
    PhylomeDBi P13596.

    Miscellaneous databases

    EvolutionaryTracei P13596.
    NextBioi 603762.

    Gene expression databases

    Genevestigatori P13596.

    Family and domain databases

    Gene3Di 2.60.40.10. 7 hits.
    InterProi IPR003961. Fibronectin_type3.
    IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR013098. Ig_I-set.
    IPR003598. Ig_sub2.
    IPR009138. Neural_cell_adh.
    [Graphical view ]
    Pfami PF00041. fn3. 2 hits.
    PF07679. I-set. 5 hits.
    [Graphical view ]
    PRINTSi PR01838. NCAMFAMILY.
    SMARTi SM00060. FN3. 2 hits.
    SM00408. IGc2. 5 hits.
    [Graphical view ]
    SUPFAMi SSF49265. SSF49265. 1 hit.
    PROSITEi PS50853. FN3. 2 hits.
    PS50835. IG_LIKE. 5 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of a cDNA clone that contains the complete coding sequence for a 140-kD rat NCAM polypeptide."
      Small S.J., Shull G.E., Santoni M.-J., Akeson R.
      J. Cell Biol. 105:2335-2345(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "Expression of the unique NCAM VASE exon is independently regulated in distinct tissues during development."
      Small S.J., Akeson R.
      J. Cell Biol. 111:2089-2096(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE OF 340-381.
    3. "Polypeptide variation in an N-CAM extracellular immunoglobulin-like fold is developmentally regulated through alternative splicing."
      Small S.J., Haines S.L., Akeson R.A.
      Neuron 1:1007-1017(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 355-364.
    4. Lubec G., Kang S.U.
      Submitted (JUL-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 38-48 AND 594-605, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Brain.

    Entry informationi

    Entry nameiNCAM1_RAT
    AccessioniPrimary (citable) accession number: P13596
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: January 1, 1990
    Last modified: October 1, 2014
    This is version 137 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3