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Protein

Neural cell adhesion molecule 1

Gene

Ncam1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.

GO - Molecular functioni

GO - Biological processi

  • axon guidance Source: Reactome
  • cell surface receptor signaling pathway Source: MGI
  • homotypic cell-cell adhesion Source: MGI
  • neuron projection development Source: MGI
  • positive regulation of calcium-mediated signaling Source: MGI
  • regulation of exocyst assembly Source: MGI
  • regulation of synaptic plasticity Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Heparin-binding

Enzyme and pathway databases

ReactomeiR-MMU-375165. NCAM signaling for neurite out-growth.
R-MMU-419037. NCAM1 interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Neural cell adhesion molecule 1
Short name:
N-CAM-1
Short name:
NCAM-1
Alternative name(s):
CD_antigen: CD56
Gene namesi
Name:Ncam1
Synonyms:Ncam
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:97281. Ncam1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini20 – 711ExtracellularSequence analysisAdd BLAST692
Transmembranei712 – 729HelicalSequence analysisAdd BLAST18
Topological domaini730 – 1115CytoplasmicSequence analysisAdd BLAST386

GO - Cellular componenti

  • anchored component of membrane Source: UniProtKB-KW
  • axon Source: MGI
  • cell-cell junction Source: MGI
  • cell surface Source: MGI
  • cytoplasm Source: MGI
  • external side of plasma membrane Source: MGI
  • extracellular exosome Source: MGI
  • growth cone Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: MGI
  • myelin sheath Source: UniProtKB
  • neuronal cell body Source: MGI
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 191 PublicationAdd BLAST19
ChainiPRO_000001501220 – 1115Neural cell adhesion molecule 1Add BLAST1096

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi41 ↔ 96Combined sources1 Publication
Disulfide bondi139 ↔ 189Combined sources1 Publication
Glycosylationi222N-linked (GlcNAc...); partial1 Publication1
Disulfide bondi235 ↔ 288Curated
Glycosylationi316N-linked (GlcNAc...)1 Publication1
Disulfide bondi330 ↔ 386Curated
Glycosylationi348N-linked (GlcNAc...)1 Publication1
Glycosylationi424N-linked (GlcNAc...)1 Publication1
Disulfide bondi427 ↔ 480Curated
Glycosylationi450N-linked (GlcNAc...)3 Publications1
Glycosylationi453N-linked (GlcNAc...); atypical1 Publication1
Glycosylationi479N-linked (GlcNAc...)1 Publication1
Modified residuei770PhosphoserineCombined sources1
Modified residuei774PhosphoserineCombined sources1
Modified residuei887PhosphoserineCombined sources1
Modified residuei890PhosphoserineCombined sources1
Modified residuei926PhosphoserineCombined sources1
Modified residuei929PhosphothreonineCombined sources1
Modified residuei946PhosphoserineCombined sources1
Modified residuei958PhosphoserineCombined sources1
Modified residuei1001PhosphothreonineCombined sources1
Modified residuei1005PhosphoserineCombined sources1
Modified residuei1030PhosphothreonineCombined sources1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

Proteomic databases

MaxQBiP13595.
PaxDbiP13595.
PeptideAtlasiP13595.
PRIDEiP13595.

PTM databases

iPTMnetiP13595.
PhosphoSitePlusiP13595.
SwissPalmiP13595.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Fgfr2P218032EBI-774943,EBI-6286942

Protein-protein interaction databases

BioGridi201699. 3 interactors.
IntActiP13595. 6 interactors.
MINTiMINT-1176881.
STRINGi10090.ENSMUSP00000130668.

Structurei

Secondary structure

11115
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi21 – 32Combined sources12
Beta strandi37 – 44Combined sources8
Beta strandi47 – 49Combined sources3
Beta strandi51 – 56Combined sources6
Turni57 – 59Combined sources3
Beta strandi64 – 73Combined sources10
Beta strandi75 – 77Combined sources3
Beta strandi79 – 85Combined sources7
Turni88 – 90Combined sources3
Beta strandi92 – 99Combined sources8
Beta strandi101 – 103Combined sources3
Beta strandi105 – 115Combined sources11
Beta strandi119 – 122Combined sources4
Beta strandi125 – 128Combined sources4
Beta strandi133 – 137Combined sources5
Beta strandi140 – 143Combined sources4
Beta strandi145 – 157Combined sources13
Helixi158 – 161Combined sources4
Beta strandi166 – 168Combined sources3
Beta strandi174 – 178Combined sources5
Beta strandi185 – 193Combined sources9
Turni194 – 197Combined sources4
Beta strandi198 – 207Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2NCMNMR-A20-116[»]
3NCMNMR-A119-208[»]
ProteinModelPortaliP13595.
SMRiP13595.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP13595.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 111Ig-like C2-type 1Add BLAST92
Domaini116 – 205Ig-like C2-type 2Add BLAST90
Domaini212 – 302Ig-like C2-type 3Add BLAST91
Domaini309 – 402Ig-like C2-type 4Add BLAST94
Domaini407 – 492Ig-like C2-type 5Add BLAST86
Domaini500 – 599Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST100
Domaini601 – 696Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST96

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni152 – 156Heparin-bindingSequence analysis5
Regioni161 – 165Heparin-bindingSequence analysis5

Sequence similaritiesi

Contains 2 fibronectin type-III domains.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IQJI. Eukaryota.
ENOG410XTGB. LUCA.
HOVERGENiHBG052579.
InParanoidiP13595.
KOiK06491.
PhylomeDBiP13595.

Family and domain databases

CDDicd00063. FN3. 2 hits.
Gene3Di2.60.40.10. 7 hits.
InterProiIPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013106. Ig_V-set.
IPR033019. Ncam1.
IPR009138. Neural_cell_adh.
[Graphical view]
PANTHERiPTHR10489:SF34. PTHR10489:SF34. 2 hits.
PfamiPF00041. fn3. 2 hits.
PF07679. I-set. 3 hits.
[Graphical view]
PRINTSiPR01838. NCAMFAMILY.
SMARTiSM00060. FN3. 2 hits.
SM00409. IG. 5 hits.
SM00408. IGc2. 5 hits.
SM00406. IGv. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 5 hits.
SSF49265. SSF49265. 1 hit.
PROSITEiPS50853. FN3. 2 hits.
PS50835. IG_LIKE. 5 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P13595-1) [UniParc]FASTAAdd to basket
Also known as: N-CAM 180

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLRTKDLIWT LFFLGTAVSL QVDIVPSQGE ISVGESKFFL CQVAGDAKDK
60 70 80 90 100
DISWFSPNGE KLSPNQQRIS VVWNDDDSST LTIYNANIDD AGIYKCVVTA
110 120 130 140 150
EDGTQSEATV NVKIFQKLMF KNAPTPQEFK EGEDAVIVCD VVSSLPPTII
160 170 180 190 200
WKHKGRDVIL KKDVRFIVLS NNYLQIRGIK KTDEGTYRCE GRILARGEIN
210 220 230 240 250
FKDIQVIVNV PPTVQARQSI VNATANLGQS VTLVCDADGF PEPTMSWTKD
260 270 280 290 300
GEPIENEEED DEKHIFSDDS SELTIRNVDK NDEAEYVCIA ENKAGEQDAS
310 320 330 340 350
IHLKVFAKPK ITYVENQTAM ELEEQVTLTC EASGDPIPSI TWRTSTRNIS
360 370 380 390 400
SEEKTLDGHM VVRSHARVSS LTLKSIQYTD AGEYICTASN TIGQDSQSMY
410 420 430 440 450
LEFQYAPKLQ GPVAVYTWEG NQVNITCEVF AYPSATISWF RDGQLLPSSN
460 470 480 490 500
YSNIKIYNTP SASYLEVTPD SENDFGNYNC TAVNRIGQES LEFILVQADT
510 520 530 540 550
PSSPSIDRVE PYSSTAQVQF DEPEATGGVP ILKYKAEWKS LGEESWHFKW
560 570 580 590 600
YDAKEANMEG IVTIMGLKPE TRYSVRLAAL NGKGLGEISA ATEFKTQPVR
610 620 630 640 650
EPSAPKLEGQ MGEDGNSIKV NLIKQDDGGS PIRHYLVKYR ALASEWKPEI
660 670 680 690 700
RLPSGSDHVM LKSLDWNAEY EVYVVAENQQ GKSKAAHFVF RTSAQPTAIP
710 720 730 740 750
ANGSPTAGLS TGAIVGILIV IFVLLLVVMD ITCYFLNKCG LLMCIAVNLC
760 770 780 790 800
GKAGPGAKGK DMEEGKAAFS KDESKEPIVE VRTEEERTPN HDGGKHTEPN
810 820 830 840 850
ETTPLTEPEL PADTTATVED MLPSVTTVTT NSDTITETFA TAQNSPTSET
860 870 880 890 900
TTLTSSIAPP ATTVPDSNSV PAGQATPSKG VTASSSSPAS APKVAPLVDL
910 920 930 940 950
SDTPTSAPSA SNLSSTVLAN QGAVLSPSTP ASAGETSKAP PASKASPAPT
960 970 980 990 1000
PTPAGAASPL AAVAAPATDA PQAKQEAPST KGPDPEPTQP GTVKNPPEAA
1010 1020 1030 1040 1050
TAPASPKSKA ATTNPSQGED LKMDEGNFKT PDIDLAKDVF AALGSPRPAT
1060 1070 1080 1090 1100
GASGQASELA PSPADSAVPP APAKTEKGPV ETKSEPPESE AKPAPTEVKT
1110
VPNDATQTKE NESKA
Length:1,115
Mass (Da):119,427
Last modified:July 22, 2008 - v3
Checksum:i78AF831BABD23918
GO
Isoform 2 (identifier: P13595-2) [UniParc]FASTAAdd to basket
Also known as: N-CAM 140

The sequence of this isoform differs from the canonical sequence as follows:
     810-1076: Missing.

Show »
Length:848
Mass (Da):93,540
Checksum:i4D77D553F474AD8E
GO
Isoform 3 (identifier: P13595-3) [UniParc] [UniParc]FASTAAdd to basket
Also known as: N-CAM 120

The sequence of this isoform differs from the canonical sequence as follows:
     702-725: NGSPTAGLSTGAIVGILIVIFVLL → TLGGSSTSYTLVSLLFSAVTLLLL
     726-1115: Missing.

Note: GPI-anchor amidated serine at position Ser-706.
Show »
Length:725
Mass (Da):80,406
Checksum:i97867C2A61594CC4
GO
Isoform 4 (identifier: P13595-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     601-605: EPSAP → KSSLF
     606-1115: Missing.

Show »
Length:605
Mass (Da):67,353
Checksum:iD497D834C34EAEC0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti20L → M in CAA33148 (PubMed:2721486).Curated1
Sequence conflicti158V → F in AAH11310 (PubMed:15489334).Curated1
Sequence conflicti261 – 268DEKHIFSD → ERSRSSVS in CAA68263 (PubMed:3595563).Curated8
Sequence conflicti273L → V in CAA68263 (PubMed:3595563).Curated1
Sequence conflicti354 – 355KT → QD in CAA68263 (PubMed:3595563).Curated2
Sequence conflicti379T → R in CAA68263 (PubMed:3595563).Curated1
Sequence conflicti379T → R in CAA33148 (PubMed:2721486).Curated1
Sequence conflicti385I → M in CAA68263 (PubMed:3595563).Curated1
Sequence conflicti385I → M in CAA33148 (PubMed:2721486).Curated1
Sequence conflicti399 – 400MY → ID in CAA68263 (PubMed:3595563).Curated2
Sequence conflicti399 – 400MY → ID in CAA33148 (PubMed:2721486).Curated2
Sequence conflicti403F → V in AAH11310 (PubMed:15489334).Curated1
Sequence conflicti403F → V in BAC34554 (PubMed:16141072).Curated1
Sequence conflicti403F → V in BAC38551 (PubMed:16141072).Curated1
Sequence conflicti549K → T in CAA68263 (PubMed:3595563).Curated1
Sequence conflicti572R → T in CAA68263 (PubMed:3595563).Curated1
Sequence conflicti575V → D in CAA68263 (PubMed:3595563).Curated1
Sequence conflicti589 – 594SAATEF → MQPSES in CAA68263 (PubMed:3595563).Curated6
Sequence conflicti600 – 602REP → PEL in CAA68263 (PubMed:3595563).Curated3
Sequence conflicti657D → H in CAA68263 (PubMed:3595563).Curated1
Sequence conflicti733C → W in BAC34554 (PubMed:16141072).Curated1
Sequence conflicti1082T → A in BAC34554 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_034826601 – 605EPSAP → KSSLF in isoform 4. 1 Publication5
Alternative sequenceiVSP_034827606 – 1115Missing in isoform 4. 1 PublicationAdd BLAST510
Alternative sequenceiVSP_034828702 – 725NGSPT…IFVLL → TLGGSSTSYTLVSLLFSAVT LLLL in isoform 3. 1 PublicationAdd BLAST24
Alternative sequenceiVSP_034829726 – 1115Missing in isoform 3. 1 PublicationAdd BLAST390
Alternative sequenceiVSP_002588810 – 1076Missing in isoform 2. 3 PublicationsAdd BLAST267

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00051 mRNA. Translation: CAA68263.1.
BC011310 mRNA. Translation: AAH11310.1.
AK051197 mRNA. Translation: BAC34554.2.
AK082621 mRNA. Translation: BAC38551.2.
X15049 mRNA. Translation: CAA33148.1.
X15051 mRNA. Translation: CAA33150.1.
X15052 mRNA. Translation: CAA33151.1.
X06328 mRNA. Translation: CAA29641.1.
X07195 Genomic DNA. Translation: CAA30173.1.
X07197 Genomic DNA. Translation: CAA30175.1.
X07198 Genomic DNA. Translation: CAB40820.1.
X07200 Genomic DNA. Translation: CAA30177.1.
X07244 mRNA. Translation: CAA30230.1.
CCDSiCCDS40617.1. [P13595-2]
CCDS40618.1. [P13595-3]
PIRiA29673. IJMSNL.
RefSeqiNP_001074914.1. NM_001081445.1.
NP_001106675.1. NM_001113204.1.
NP_001297994.1. NM_001311065.1.
NP_035005.2. NM_010875.3.
UniGeneiMm.439182.
Mm.4974.

Genome annotation databases

GeneIDi17967.
KEGGimmu:17967.
UCSCiuc009pje.2. mouse. [P13595-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00051 mRNA. Translation: CAA68263.1.
BC011310 mRNA. Translation: AAH11310.1.
AK051197 mRNA. Translation: BAC34554.2.
AK082621 mRNA. Translation: BAC38551.2.
X15049 mRNA. Translation: CAA33148.1.
X15051 mRNA. Translation: CAA33150.1.
X15052 mRNA. Translation: CAA33151.1.
X06328 mRNA. Translation: CAA29641.1.
X07195 Genomic DNA. Translation: CAA30173.1.
X07197 Genomic DNA. Translation: CAA30175.1.
X07198 Genomic DNA. Translation: CAB40820.1.
X07200 Genomic DNA. Translation: CAA30177.1.
X07244 mRNA. Translation: CAA30230.1.
CCDSiCCDS40617.1. [P13595-2]
CCDS40618.1. [P13595-3]
PIRiA29673. IJMSNL.
RefSeqiNP_001074914.1. NM_001081445.1.
NP_001106675.1. NM_001113204.1.
NP_001297994.1. NM_001311065.1.
NP_035005.2. NM_010875.3.
UniGeneiMm.439182.
Mm.4974.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2NCMNMR-A20-116[»]
3NCMNMR-A119-208[»]
ProteinModelPortaliP13595.
SMRiP13595.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201699. 3 interactors.
IntActiP13595. 6 interactors.
MINTiMINT-1176881.
STRINGi10090.ENSMUSP00000130668.

PTM databases

iPTMnetiP13595.
PhosphoSitePlusiP13595.
SwissPalmiP13595.

Proteomic databases

MaxQBiP13595.
PaxDbiP13595.
PeptideAtlasiP13595.
PRIDEiP13595.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi17967.
KEGGimmu:17967.
UCSCiuc009pje.2. mouse. [P13595-1]

Organism-specific databases

CTDi4684.
MGIiMGI:97281. Ncam1.

Phylogenomic databases

eggNOGiENOG410IQJI. Eukaryota.
ENOG410XTGB. LUCA.
HOVERGENiHBG052579.
InParanoidiP13595.
KOiK06491.
PhylomeDBiP13595.

Enzyme and pathway databases

ReactomeiR-MMU-375165. NCAM signaling for neurite out-growth.
R-MMU-419037. NCAM1 interactions.

Miscellaneous databases

ChiTaRSiNcam1. mouse.
EvolutionaryTraceiP13595.
PROiP13595.
SOURCEiSearch...

Family and domain databases

CDDicd00063. FN3. 2 hits.
Gene3Di2.60.40.10. 7 hits.
InterProiIPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013106. Ig_V-set.
IPR033019. Ncam1.
IPR009138. Neural_cell_adh.
[Graphical view]
PANTHERiPTHR10489:SF34. PTHR10489:SF34. 2 hits.
PfamiPF00041. fn3. 2 hits.
PF07679. I-set. 3 hits.
[Graphical view]
PRINTSiPR01838. NCAMFAMILY.
SMARTiSM00060. FN3. 2 hits.
SM00409. IG. 5 hits.
SM00408. IGc2. 5 hits.
SM00406. IGv. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 5 hits.
SSF49265. SSF49265. 1 hit.
PROSITEiPS50853. FN3. 2 hits.
PS50835. IG_LIKE. 5 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNCAM1_MOUSE
AccessioniPrimary (citable) accession number: P13595
Secondary accession number(s): P13594
, Q61949, Q61950, Q6LBU8, Q8BQ96, Q8C4B2, Q921P2, Q9R2A7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: July 22, 2008
Last modified: November 30, 2016
This is version 172 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.