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P13591 (NCAM1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 154. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (7) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Neural cell adhesion molecule 1

Short name=N-CAM-1
Short name=NCAM-1
Alternative name(s):
CD_antigen=CD56
Gene names
Name:NCAM1
Synonyms:NCAM
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length858 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.

Subcellular location

Isoform 1: Cell membrane; Single-pass type I membrane protein.

Isoform 2: Cell membrane; Single-pass type I membrane protein.

Isoform 3: Cell membrane; Lipid-anchorGPI-anchor.

Isoform 4: Cell membrane; Lipid-anchorGPI-anchor Potential.

Isoform 5: Secreted.

Isoform 6: Secreted Potential.

Sequence similarities

Contains 2 fibronectin type-III domains.

Contains 5 Ig-like C2-type (immunoglobulin-like) domains.

Sequence caution

The sequence BAD92680.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P13591-2)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P13591-1)

Also known as: N-CAM 140;

The sequence of this isoform differs from the canonical sequence as follows:
     354-363: Missing.
Isoform 3 (identifier: P13591-3)

Also known as: N-CAM 120;

The sequence of this isoform differs from the canonical sequence as follows:
     354-363: Missing.
     609-609: Q → HSPPPPASASSSTPVPLSPPDTTWPLPALATTEPAK
     712-736: NGSPTSGLSTGAIVGILIVIFVLLL → TLGGNSASYTFVSLLFSAVTLLLLC
     737-858: Missing.
Note: GPI-anchor amidated asparagine on Asn-741.
Isoform 4 (identifier: P13591-4)

The sequence of this isoform differs from the canonical sequence as follows:
     354-363: Missing.
     712-736: NGSPTSGLSTGAIVGILIVIFVLLL → TLGGNSASYTFVSLLFSAVTLLLLC
     737-858: Missing.
Note: GPI-anchor amidated asparagine on Asn-706.
Isoform 5 (identifier: P13591-5)

Also known as: C;

The sequence of this isoform differs from the canonical sequence as follows:
     609-665: QGEPSAPKLE...WKPEIRLPSG → HSPPPPASAS...QAGLHNALMK
     666-858: Missing.
Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Isoform 6 (identifier: P13591-6)

The sequence of this isoform differs from the canonical sequence as follows:
     364-364: T → V
     365-858: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 By similarity
Chain20 – 858839Neural cell adhesion molecule 1
PRO_0000015009

Regions

Topological domain20 – 718699Extracellular Potential
Transmembrane719 – 73921Helical; Potential
Topological domain740 – 858119Cytoplasmic Potential
Domain20 – 11192Ig-like C2-type 1
Domain116 – 20590Ig-like C2-type 2
Domain212 – 30190Ig-like C2-type 3
Domain308 – 413106Ig-like C2-type 4
Domain416 – 50186Ig-like C2-type 5
Domain509 – 608100Fibronectin type-III 1
Domain611 – 70696Fibronectin type-III 2

Amino acid modifications

Modified residue7841Phosphoserine By similarity
Glycosylation2221N-linked (GlcNAc...) Potential
Glycosylation3151N-linked (GlcNAc...) Potential
Glycosylation3471N-linked (GlcNAc...) Potential
Glycosylation4331N-linked (GlcNAc...) Potential
Glycosylation4591N-linked (GlcNAc...) Ref.10
Glycosylation4881N-linked (GlcNAc...) Potential
Disulfide bond41 ↔ 96 Probable
Disulfide bond139 ↔ 189 Probable
Disulfide bond235 ↔ 287 Probable
Disulfide bond329 ↔ 395 Probable
Disulfide bond436 ↔ 489 Probable

Natural variations

Alternative sequence354 – 36310Missing in isoform 2, isoform 3 and isoform 4.
VSP_034818
Alternative sequence3641T → V in isoform 6.
VSP_034819
Alternative sequence365 – 858494Missing in isoform 6.
VSP_034820
Alternative sequence609 – 66557QGEPS…RLPSG → HSPPPPASASSSTPVPLSPP DTTWPLPALATTEPAKNIAQ NHCCNMFQAGLHNALMK in isoform 5.
VSP_034821
Alternative sequence6091Q → HSPPPPASASSSTPVPLSPP DTTWPLPALATTEPAK in isoform 3.
VSP_034822
Alternative sequence666 – 858193Missing in isoform 5.
VSP_034823
Alternative sequence712 – 73625NGSPT…FVLLL → TLGGNSASYTFVSLLFSAVT LLLLC in isoform 3 and isoform 4.
VSP_034824
Alternative sequence737 – 858122Missing in isoform 3 and isoform 4.
VSP_034825
Natural variant71L → F.
Corresponds to variant rs7105734 [ dbSNP | Ensembl ].
VAR_061331
Natural variant2601D → N.
Corresponds to variant rs17115160 [ dbSNP | Ensembl ].
VAR_049960
Natural variant6791E → D. Ref.6
Corresponds to variant rs17115280 [ dbSNP | Ensembl ].
VAR_049961
Natural variant8341T → M.
Corresponds to variant rs17174409 [ dbSNP | Ensembl ].
VAR_049962

Experimental info

Sequence conflict2151Q → R in AAB04558. Ref.2
Sequence conflict2391G → R in AAB04558. Ref.2
Sequence conflict5001L → F in AAB04558. Ref.2
Sequence conflict5601Y → H in BAF85142. Ref.4
Sequence conflict609 – 6102QG → R in AAA59913. Ref.8
Sequence conflict730 – 7312Missing in AAA59913. Ref.8
Sequence conflict8211G → A in AAA59913. Ref.8
Sequence conflict8561S → N in AAH47244. Ref.6

Secondary structure

................................................... 858
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 22, 2008. Version 3.
Checksum: FD3B9DE80D802554

FASTA85894,574
        10         20         30         40         50         60 
MLQTKDLIWT LFFLGTAVSL QVDIVPSQGE ISVGESKFFL CQVAGDAKDK DISWFSPNGE 

        70         80         90        100        110        120 
KLTPNQQRIS VVWNDDSSST LTIYNANIDD AGIYKCVVTG EDGSESEATV NVKIFQKLMF 

       130        140        150        160        170        180 
KNAPTPQEFR EGEDAVIVCD VVSSLPPTII WKHKGRDVIL KKDVRFIVLS NNYLQIRGIK 

       190        200        210        220        230        240 
KTDEGTYRCE GRILARGEIN FKDIQVIVNV PPTIQARQNI VNATANLGQS VTLVCDAEGF 

       250        260        270        280        290        300 
PEPTMSWTKD GEQIEQEEDD EKYIFSDDSS QLTIKKVDKN DEAEYICIAE NKAGEQDATI 

       310        320        330        340        350        360 
HLKVFAKPKI TYVENQTAME LEEQVTLTCE ASGDPIPSIT WRTSTRNISS EEKASWTRPE 

       370        380        390        400        410        420 
KQETLDGHMV VRSHARVSSL TLKSIQYTDA GEYICTASNT IGQDSQSMYL EVQYAPKLQG 

       430        440        450        460        470        480 
PVAVYTWEGN QVNITCEVFA YPSATISWFR DGQLLPSSNY SNIKIYNTPS ASYLEVTPDS 

       490        500        510        520        530        540 
ENDFGNYNCT AVNRIGQESL EFILVQADTP SSPSIDQVEP YSSTAQVQFD EPEATGGVPI 

       550        560        570        580        590        600 
LKYKAEWRAV GEEVWHSKWY DAKEASMEGI VTIVGLKPET TYAVRLAALN GKGLGEISAA 

       610        620        630        640        650        660 
SEFKTQPVQG EPSAPKLEGQ MGEDGNSIKV NLIKQDDGGS PIRHYLVRYR ALSSEWKPEI 

       670        680        690        700        710        720 
RLPSGSDHVM LKSLDWNAEY EVYVVAENQQ GKSKAAHFVF RTSAQPTAIP ANGSPTSGLS 

       730        740        750        760        770        780 
TGAIVGILIV IFVLLLVVVD ITCYFLNKCG LFMCIAVNLC GKAGPGAKGK DMEEGKAAFS 

       790        800        810        820        830        840 
KDESKEPIVE VRTEEERTPN HDGGKHTEPN ETTPLTEPEK GPVEAKPECQ ETETKPAPAE 

       850 
VKTVPNDATQ TKENESKA 

« Hide

Isoform 2 (N-CAM 140) [UniParc].

Checksum: 68D2F0C0E6C1C2AD
Show »

FASTA84893,361
Isoform 3 (N-CAM 120) [UniParc] [UniParc].

Checksum: F0CAD3292D7AB67E
Show »

FASTA76183,770
Isoform 4 [UniParc].

Checksum: 1B6D55D0BF65200F
Show »

FASTA72680,312
Isoform 5 (C) [UniParc] [UniParc].

Checksum: E496F232C162C614
Show »

FASTA66573,473
Isoform 6 [UniParc].

Checksum: AC697008755FE6FD
Show »

FASTA36440,760

References

« Hide 'large scale' references
[1]"Complete sequence and in vitro expression of a tissue-specific phosphatidylinositol-linked N-CAM isoform from skeletal muscle."
Barton C.H., Dickson G., Gower H.J., Rowett L.H., Putt W., Elsom V., Moore S.E., Goridis C., Walsh F.S.
Development 104:165-173(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
Tissue: Skeletal muscle.
[2]"Molecular and functional analysis of human natural killer cell-associated neural cell adhesion molecule (N-CAM/CD56)."
Lanier L.L., Chang C., Azuma M., Ruitenberg J.J., Hemperly J.J., Phillips J.H.
J. Immunol. 146:4421-4426(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[3]"Complementary DNA sequence encoding the major neural cell adhesion molecule isoform in a human small cell lung cancer cell line."
Saito S., Tanio Y., Tachibana I., Hayashi S., Kishimoto T., Kawase I.
Lung Cancer 10:307-318(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Testis.
[5]Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F.
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
Tissue: Brain.
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 6), VARIANT ASP-679.
Tissue: Brain, Kidney and Ovary.
[7]"Splicing of the VASE exon of neural cell adhesion molecule (NCAM) in human small-cell lung carcinoma (SCLC)."
van Duijnhoven H.L., Helfrich W., de Leij L., Roebroek A.J., van de Ven W.J., Healey K., Culverwell A., Rossell R.J., Kemshead J.T., Patel K.
Int. J. Cancer 50:118-123(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 347-369.
[8]"Human muscle neural cell adhesion molecule (N-CAM): identification of a muscle-specific sequence in the extracellular domain."
Dickson G., Gower H.J., Barton C.H., Prentice H.M., Elsom V.L., Moore S.E., Cox R.D., Quinn C., Putt W., Walsh F.S.
Cell 50:1119-1130(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 501-858 (ISOFORMS 1/2 AND 3).
Tissue: Skeletal muscle.
[9]"Alternative splicing generates a secreted form of N-CAM in muscle and brain."
Gower H.J., Barton C.H., Elsom V.L., Thompson J., Moore S.E., Dickson G., Walsh F.S.
Cell 55:955-964(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 501-858 (ISOFORM 5).
[10]"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."
Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D.
J. Proteome Res. 4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-459.
Tissue: Plasma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X16841 mRNA. Translation: CAA34739.1.
U63041 mRNA. Translation: AAB04558.1.
S71824 mRNA. Translation: AAB31836.1.
AK292453 mRNA. Translation: BAF85142.1.
AB209443 mRNA. Translation: BAD92680.1. Different initiation.
BC014205 mRNA. Translation: AAH14205.2.
BC029119 mRNA. Translation: AAH29119.1.
BC047244 mRNA. Translation: AAH47244.1.
S73101 mRNA. Translation: AAB20698.1.
M17410 mRNA. Translation: AAA59913.1.
M17409 mRNA. Translation: AAA59912.1.
M22094 mRNA. Translation: AAA59910.1.
M22092, M22091 Genomic DNA. Translation: AAA59911.1.
PIRA31635.
I54773.
IJHUNG. S07784.
RefSeqNP_000606.3. NM_000615.6.
NP_001070150.1. NM_001076682.3.
NP_001229537.1. NM_001242608.1.
NP_851996.2. NM_181351.4.
UniGeneHs.503878.
Hs.711235.
Hs.733031.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2E3VX-ray1.95A/B/C510-608[»]
2HAZX-ray1.70A508-608[»]
2VKWX-ray2.30A/B506-702[»]
2VKXX-ray2.70A/B/C/D/E/F506-702[»]
3MTRX-ray1.80A/B414-611[»]
ProteinModelPortalP13591.
SMRP13591. Positions 20-706.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid110764. 12 interactions.
DIPDIP-59530N.
IntActP13591. 7 interactions.
MINTMINT-4103137.

PTM databases

PhosphoSiteP13591.

Polymorphism databases

DMDM205830665.

Proteomic databases

PaxDbP13591.
PRIDEP13591.

Protocols and materials databases

DNASU4684.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000531817; ENSP00000475074; ENSG00000149294.
ENST00000576604; ENSP00000459932; ENSG00000262279. [P13591-2]
ENST00000590031; ENSP00000466544; ENSG00000262279. [P13591-1]
ENST00000591218; ENSP00000465154; ENSG00000262279. [P13591-6]
ENST00000592004; ENSP00000467650; ENSG00000262279.
ENST00000592855; ENSP00000467282; ENSG00000262279. [P13591-4]
ENST00000593059; ENSP00000466769; ENSG00000262279. [P13591-3]
GeneID4684.
KEGGhsa:4684.
UCSCuc001pno.3. human. [P13591-6]
uc001pnp.3. human. [P13591-3]
uc001pnq.3. human. [P13591-2]
uc001pnr.3. human. [P13591-1]
uc021qqo.1. human. [P13591-4]

Organism-specific databases

CTD4684.
GeneCardsGC11P112831.
HGNCHGNC:7656. NCAM1.
HPACAB000142.
CAB018071.
HPA039835.
MIM116930. gene.
neXtProtNX_P13591.
PharmGKBPA31459.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG308439.
HOVERGENHBG052579.
InParanoidP13591.
KOK06491.
TreeFamTF326195.

Enzyme and pathway databases

ReactomeREACT_111045. Developmental Biology.
REACT_118779. Extracellular matrix organization.
REACT_6900. Immune System.

Gene expression databases

ArrayExpressP13591.
BgeeP13591.
GenevestigatorP13591.

Family and domain databases

Gene3D2.60.40.10. 7 hits.
InterProIPR003961. Fibronectin_type3.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003598. Ig_sub2.
IPR009138. Neural_cell_adh.
[Graphical view]
PfamPF00041. fn3. 2 hits.
PF07679. I-set. 5 hits.
[Graphical view]
PRINTSPR01838. NCAMFAMILY.
SMARTSM00060. FN3. 2 hits.
SM00408. IGc2. 5 hits.
[Graphical view]
SUPFAMSSF49265. SSF49265. 1 hit.
PROSITEPS50853. FN3. 2 hits.
PS50835. IG_LIKE. 5 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSNCAM1. human.
EvolutionaryTraceP13591.
GeneWikiNeural_cell_adhesion_molecule.
GenomeRNAi4684.
NextBio18060.
PROP13591.
SOURCESearch...

Entry information

Entry nameNCAM1_HUMAN
AccessionPrimary (citable) accession number: P13591
Secondary accession number(s): A8K8T8 expand/collapse secondary AC list , P13592, P13593, Q05C58, Q15829, Q16180, Q16209, Q59FL7, Q86X47, Q96CJ3
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: July 22, 2008
Last modified: March 19, 2014
This is version 154 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM

Human cell differentiation molecules

CD nomenclature of surface proteins of human leucocytes and list of entries