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Protein

Neural cell adhesion molecule 1

Gene

NCAM1

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Names & Taxonomyi

Protein namesi
Recommended name:
Neural cell adhesion molecule 1
Short name:
N-CAM-1
Short name:
NCAM-1
Gene namesi
Name:NCAM1
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 711692ExtracellularSequence analysisAdd
BLAST
Transmembranei712 – 72918HelicalSequence analysisAdd
BLAST
Topological domaini730 – 1091362CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Add
BLAST
Chaini20 – 10911072Neural cell adhesion molecule 1PRO_0000015007Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi41 ↔ 96PROSITE-ProRule annotation
Disulfide bondi139 ↔ 189PROSITE-ProRule annotation
Glycosylationi222 – 2221N-linked (GlcNAc...)
Disulfide bondi235 ↔ 287Combined sources1 Publication
Glycosylationi315 – 3151N-linked (GlcNAc...)Sequence analysis
Disulfide bondi329 ↔ 385PROSITE-ProRule annotation
Glycosylationi347 – 3471N-linked (GlcNAc...)Sequence analysis
Glycosylationi423 – 4231N-linked (GlcNAc...)Sequence analysis
Disulfide bondi426 ↔ 479PROSITE-ProRule annotation
Glycosylationi449 – 4491N-linked (GlcNAc...)Sequence analysis
Glycosylationi478 – 4781N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP13590.
PRIDEiP13590.

Interactioni

Protein-protein interaction databases

IntActiP13590. 1 interaction.
STRINGi9031.ENSGALP00000012710.

Structurei

Secondary structure

1
1091
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi203 – 2064Combined sources
Beta strandi210 – 2167Combined sources
Beta strandi219 – 2246Combined sources
Beta strandi229 – 2335Combined sources
Beta strandi235 – 2417Combined sources
Beta strandi244 – 2496Combined sources
Turni256 – 2583Combined sources
Beta strandi267 – 2693Combined sources
Beta strandi272 – 2776Combined sources
Beta strandi283 – 2908Combined sources
Beta strandi295 – 30511Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1IE5NMR-A202-307[»]
ProteinModelPortaliP13590.
SMRiP13590. Positions 20-307, 496-693.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP13590.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini20 – 11394Ig-like C2-type 1Add
BLAST
Domaini116 – 20590Ig-like C2-type 2Add
BLAST
Domaini212 – 30190Ig-like C2-type 3Add
BLAST
Domaini308 – 40396Ig-like C2-type 4Add
BLAST
Domaini406 – 49590Ig-like C2-type 5Add
BLAST
Domaini499 – 598100Fibronectin type-III 1PROSITE-ProRule annotationAdd
BLAST
Domaini600 – 69697Fibronectin type-III 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni152 – 1565Heparin-bindingSequence analysis
Regioni161 – 1655Heparin-bindingSequence analysis

Sequence similaritiesi

Contains 2 fibronectin type-III domains.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IQJI. Eukaryota.
ENOG410XTGB. LUCA.
HOGENOMiHOG000074124.
HOVERGENiHBG052579.
InParanoidiP13590.
PhylomeDBiP13590.

Family and domain databases

Gene3Di2.60.40.10. 7 hits.
InterProiIPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR033019. Ncam1.
IPR009138. Neural_cell_adh.
[Graphical view]
PANTHERiPTHR10489:SF34. PTHR10489:SF34. 1 hit.
PfamiPF00041. fn3. 2 hits.
PF07679. I-set. 3 hits.
[Graphical view]
PRINTSiPR01838. NCAMFAMILY.
SMARTiSM00060. FN3. 2 hits.
SM00409. IG. 5 hits.
SM00408. IGc2. 5 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 5 hits.
SSF49265. SSF49265. 1 hit.
PROSITEiPS50853. FN3. 2 hits.
PS50835. IG_LIKE. 5 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: P13590-1) [UniParc]FASTAAdd to basket

Also known as: N-CAM 180

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLPAAALPWT LFFLGAAASL QVDIVPSQGE ISVGESKFFL CQVAGEAKYK
60 70 80 90 100
DISWFSPNGE KLTPNQQRIS VVRNDDFSST LTIYNANIDD AGIYKCVVSS
110 120 130 140 150
VEEGDSEATV NVKIFQKLMF KNAPTPQEFK EGDDAVIVCD VVSSLPPTII
160 170 180 190 200
WKHKGRDVML KKDVRFIVLS NNYLQIRGIK KTDEGTYRCE GRILARGEIN
210 220 230 240 250
FKDIQVIVNV PPSVRARQST MNATANLSQS VTLACDADGF PEPTMTWTKD
260 270 280 290 300
GEPIEQEDNE EKYSFNYDGS ELIIKKVDKS DEAEYICIAE NKAGEQDATI
310 320 330 340 350
HLKVFAKPKI TYVENKTAME LEDQITLTCE ASGDPIPSIT WKTSTRNISN
360 370 380 390 400
EEKTLDGRIV VRSHARVSSL TLKEIQYTDA GEYVCTASNT IGQDSQAMYL
410 420 430 440 450
EVQYAPKLQG PVAVYTWEGN QVNITCEVFA YPSAVISWFR DGQLLPSSNY
460 470 480 490 500
SNIKIYNTPS ASYLEVTPDS ENDFGNYNCT AVNRIGQESS EFILVQADTP
510 520 530 540 550
SSPSIDRVEP YSSTARVEFD EPEATGGVPI LKYKAEWRAL GEGEWHSRLY
560 570 580 590 600
DAKEANVEGT ITISGLKPET TYSVRLSAVN GKGVGEISLP SDFKTQPVRE
610 620 630 640 650
PSAPKLEGQM GEDGNSIKVN VIKQDDGGSP IRHYLIKYKA KHSSEWKPEI
660 670 680 690 700
RLPSGIDHVM LKSLDWNAEY EVYVIAENQQ GKSKPAHYAF RTSAQPTVIP
710 720 730 740 750
ASTSPTSGLG TAAIVGILIV IFVLLLVAVD VTCYFLNKCG LLMCIAVNLC
760 770 780 790 800
GKSGPGAKGK DMEEGKAAFS KDESKEPIVE VRTEEERTPN HDGGKHTEPN
810 820 830 840 850
ETTPLTEPEH TADTAATVED MLPSVTTGTT NSDTITETFA TAQNSPTSET
860 870 880 890 900
TTLTSSIAPP ATAIPDSNAM SPGQATPAKA GASPVSPPPP SSTPKVAPLV
910 920 930 940 950
DLSDTPSSAP ATNNLSSSVL SNQGAVLSPS TVANMAETSK AAAGNKSAAP
960 970 980 990 1000
TPANLTSPPA PSEPKQEVSS TKSPEKEAAQ PSTVKSPTET AKNPSNPKSE
1010 1020 1030 1040 1050
AASGGTTNPS QNEDFKMDEG TFKTPDIDLA KDVFAALGTT TPASVASGQA
1060 1070 1080 1090
RELASSTADS SVPAAPAKTE KTPVEDKSEV QATEIRHLQQ K
Length:1,091
Mass (Da):117,415
Last modified:July 15, 1998 - v3
Checksum:iB151367002DF88E0
GO
Isoform B (identifier: P13590-2) [UniParc]FASTAAdd to basket

Also known as: N-CAM 140

The sequence of this isoform differs from the canonical sequence as follows:
     771-809: Missing.

Show »
Length:1,052
Mass (Da):112,977
Checksum:i9177244881747480
GO
Isoform C (identifier: P13590-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     810-1070: Missing.

Show »
Length:830
Mass (Da):91,573
Checksum:iA07527ADC5CCCBD1
GO
Isoform D (identifier: P13590-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     702-726: STSPTSGLGTAAIVGILIVIFVLLL → TLGSPSTSSSFVSLLLSAVTLLLLC
     727-1091: Missing.

Show »
Length:726
Mass (Da):80,219
Checksum:iE08171FD649020C5
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei702 – 72625STSPT…FVLLL → TLGSPSTSSSFVSLLLSAVT LLLLC in isoform D. CuratedVSP_002583Add
BLAST
Alternative sequencei727 – 1091365Missing in isoform D. CuratedVSP_002584Add
BLAST
Alternative sequencei771 – 80939Missing in isoform B. CuratedVSP_002585Add
BLAST
Alternative sequencei810 – 1070261Missing in isoform C. CuratedVSP_002586Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15861
, M15860, M15922, M15923, M15924, M21178, M21179, M21180, M15929, M15930, M15931, M15932, M15933, M15934, L29437, M15935, M15937, M15938, M15939 Genomic DNA. Translation: AAB59958.1.
M15861
, M15860, M15922, M15923, M15924, M21178, M21179, M21180, M15929, M15930, M15931, M15932, M15934, L29437, M15935, M15937, M15939 Genomic DNA. Translation: AAB59959.1.
M15936
, M15860, M15922, M15923, M15924, M21178, M21179, M21180, M15929, M15930, M15931, M15932, M15934, L29437, M15935 Genomic DNA. Translation: AAB59957.1.
X04479 Genomic DNA. Translation: CAB51638.1.
X70342 Genomic DNA. Translation: CAA49807.1.
Z12128 Genomic DNA. Translation: CAA78113.1.
PIRiA43613. IJCHNL.
UniGeneiGga.35109.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15861
, M15860, M15922, M15923, M15924, M21178, M21179, M21180, M15929, M15930, M15931, M15932, M15933, M15934, L29437, M15935, M15937, M15938, M15939 Genomic DNA. Translation: AAB59958.1.
M15861
, M15860, M15922, M15923, M15924, M21178, M21179, M21180, M15929, M15930, M15931, M15932, M15934, L29437, M15935, M15937, M15939 Genomic DNA. Translation: AAB59959.1.
M15936
, M15860, M15922, M15923, M15924, M21178, M21179, M21180, M15929, M15930, M15931, M15932, M15934, L29437, M15935 Genomic DNA. Translation: AAB59957.1.
X04479 Genomic DNA. Translation: CAB51638.1.
X70342 Genomic DNA. Translation: CAA49807.1.
Z12128 Genomic DNA. Translation: CAA78113.1.
PIRiA43613. IJCHNL.
UniGeneiGga.35109.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1IE5NMR-A202-307[»]
ProteinModelPortaliP13590.
SMRiP13590. Positions 20-307, 496-693.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP13590. 1 interaction.
STRINGi9031.ENSGALP00000012710.

Proteomic databases

PaxDbiP13590.
PRIDEiP13590.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG410IQJI. Eukaryota.
ENOG410XTGB. LUCA.
HOGENOMiHOG000074124.
HOVERGENiHBG052579.
InParanoidiP13590.
PhylomeDBiP13590.

Miscellaneous databases

EvolutionaryTraceiP13590.
NextBioi20829295.
PROiP13590.

Family and domain databases

Gene3Di2.60.40.10. 7 hits.
InterProiIPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR033019. Ncam1.
IPR009138. Neural_cell_adh.
[Graphical view]
PANTHERiPTHR10489:SF34. PTHR10489:SF34. 1 hit.
PfamiPF00041. fn3. 2 hits.
PF07679. I-set. 3 hits.
[Graphical view]
PRINTSiPR01838. NCAMFAMILY.
SMARTiSM00060. FN3. 2 hits.
SM00409. IG. 5 hits.
SM00408. IGc2. 5 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 5 hits.
SSF49265. SSF49265. 1 hit.
PROSITEiPS50853. FN3. 2 hits.
PS50835. IG_LIKE. 5 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Neural cell adhesion molecule: structure, immunoglobulin-like domains, cell surface modulation, and alternative RNA splicing."
    Cunningham B.A., Hemperly J.J., Murray B.A., Prediger E.A., Brackenbury R., Edelman G.M.
    Science 236:799-806(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-175, PARTIAL PROTEIN SEQUENCE.
  2. "Sequence of a cDNA clone encoding the polysialic acid-rich and cytoplasmic domains of the neural cell adhesion molecule N-CAM."
    Hemperly J.J., Murray B.A., Edelman G.M., Cunningham B.A.
    Proc. Natl. Acad. Sci. U.S.A. 83:3037-3041(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 128-1091, PARTIAL PROTEIN SEQUENCE.
  3. "cDNA clones of the neural cell adhesion molecule (N-CAM) lacking a membrane-spanning region consistent with evidence for membrane attachment via a phosphatidylinositol intermediate."
    Hemperly J.J., Edelman G.M., Cunningham B.A.
    Proc. Natl. Acad. Sci. U.S.A. 83:9822-9826(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM D).
  4. "Cell surface modulation of the neural cell adhesion molecule resulting from alternative mRNA splicing in a tissue-specific developmental sequence."
    Murray B.A., Owens G.C., Prediger E.A., Crossin K.L., Cunningham B.A., Edelman G.M.
    J. Cell Biol. 103:1431-1439(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 810-1069.
  5. "Conserved regulatory elements in the promoter region of the N-CAM gene."
    Colwell G., Li B., Forrest D., Brackenbury R.
    Genomics 14:875-882(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-17.
  6. Sasner M., Covault J.
    Submitted (AUG-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-17.
    Strain: White leghorn.
    Tissue: Erythrocyte.
  7. "Topographic localization of the heparin-binding domain of the neural cell adhesion molecule N-CAM."
    Cole G.J., Loewy A., Cross N.V., Akeson R., Glaser L.
    J. Cell Biol. 103:1739-1744(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 20-29.
  8. "Identification of a peptide sequence involved in homophilic binding in the neural cell adhesion molecule NCAM."
    Rao Y., Wu X.F., Gariepy J., Rutishauser U., Siu C.H.
    J. Cell Biol. 118:937-949(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: CELL-BINDING.
  9. "Solution structure of the third immunoglobulin domain of the neural cell adhesion molecule N-CAM: can solution studies define the mechanism of homophilic binding?"
    Atkins A.R., Chung J., Deechongkit S., Little E.B., Edelman G.M., Wright P.E., Cunningham B.A., Dyson H.J.
    J. Mol. Biol. 311:161-172(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 202-307.

Entry informationi

Entry nameiNCAM1_CHICK
AccessioniPrimary (citable) accession number: P13590
Secondary accession number(s): Q90918, Q90919
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: July 15, 1998
Last modified: May 11, 2016
This is version 146 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.