P13590 (NCAM1_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Neural cell adhesion molecule 1
Short name=N-CAM-1
Short name=NCAM-1

Gene names

Name:

NCAM1

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	1091 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
Subcellular location	Cell membrane; Single-pass membrane protein Potential.
Sequence similarities	Contains 2 fibronectin type-III domains. Contains 5 Ig-like C2-type (immunoglobulin-like) domains.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Immunoglobulin domain Repeat Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Traceable author statement. Source: Reactome
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: P13590-1)

Also known as: N-CAM 180;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform B (identifier: P13590-2)

Also known as: N-CAM 140;

The sequence of this isoform differs from the canonical sequence as follows:
     771-809: Missing.

Isoform C (identifier: P13590-3)

The sequence of this isoform differs from the canonical sequence as follows:
     810-1070: Missing.

Isoform D (identifier: P13590-4)

The sequence of this isoform differs from the canonical sequence as follows:
     702-726: STSPTSGLGTAAIVGILIVIFVLLL → TLGSPSTSSSFVSLLLSAVTLLLLC
     727-1091: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 19

19

Chain

20 – 1091

1072

Neural cell adhesion molecule 1

PRO_0000015007

Regions

Topological domain

20 – 711

692

Extracellular Potential

Transmembrane

712 – 729

18

Helical; Potential

Topological domain

730 – 1091

362

Cytoplasmic Potential

Domain

20 – 113

94

Ig-like C2-type 1

Domain

116 – 205

90

Ig-like C2-type 2

Domain

212 – 301

90

Ig-like C2-type 3

Domain

308 – 403

96

Ig-like C2-type 4

Domain

406 – 495

90

Ig-like C2-type 5

Domain

496 – 595

100

Fibronectin type-III 1

Domain

597 – 692

96

Fibronectin type-III 2

Region

152 – 156

5

Heparin-binding Potential

Region

161 – 165

5

Heparin-binding Potential

Amino acid modifications

Glycosylation

222

1

N-linked (GlcNAc...)

Glycosylation

315

1

N-linked (GlcNAc...) Potential

Glycosylation

347

1

N-linked (GlcNAc...) Potential

Glycosylation

423

1

N-linked (GlcNAc...) Potential

Glycosylation

449

1

N-linked (GlcNAc...) Potential

Glycosylation

478

1

N-linked (GlcNAc...) Potential

Disulfide bond

41 ↔ 96

By similarity

Disulfide bond

139 ↔ 189

By similarity

Disulfide bond

235 ↔ 287

Disulfide bond

329 ↔ 385

By similarity

Disulfide bond

426 ↔ 479

By similarity

Natural variations

Alternative sequence

702 – 726

25

STSPT…FVLLL → TLGSPSTSSSFVSLLLSAVT LLLLC in isoform D.

VSP_002583

Alternative sequence

727 – 1091

365

Missing in isoform D.

VSP_002584

Alternative sequence

771 – 809

39

Missing in isoform B.

VSP_002585

Alternative sequence

810 – 1070

261

Missing in isoform C.

VSP_002586

Secondary structure

1

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1091

Helix Strand Turn

Details...

Beta strand

203 – 206

4

Beta strand

210 – 216

7

Beta strand

219 – 224

6

Beta strand

229 – 233

5

Beta strand

235 – 241

7

Beta strand

244 – 249

6

Turn

256 – 258

3

Beta strand

267 – 269

3

Beta strand

272 – 277

6

Beta strand

283 – 290

8

Beta strand

295 – 305

11

Sequences

	Sequence		Length	Mass (Da)
	Isoform A (N-CAM 180) [UniParc]. Last modified July 15, 1998. Version 3. Checksum: B151367002DF88E0	FASTA	1,091	117,415
	10 20 30 40 50 60 MLPAAALPWT LFFLGAAASL QVDIVPSQGE ISVGESKFFL CQVAGEAKYK DISWFSPNGE 70 80 90 100 110 120 KLTPNQQRIS VVRNDDFSST LTIYNANIDD AGIYKCVVSS VEEGDSEATV NVKIFQKLMF 130 140 150 160 170 180 KNAPTPQEFK EGDDAVIVCD VVSSLPPTII WKHKGRDVML KKDVRFIVLS NNYLQIRGIK 190 200 210 220 230 240 KTDEGTYRCE GRILARGEIN FKDIQVIVNV PPSVRARQST MNATANLSQS VTLACDADGF 250 260 270 280 290 300 PEPTMTWTKD GEPIEQEDNE EKYSFNYDGS ELIIKKVDKS DEAEYICIAE NKAGEQDATI 310 320 330 340 350 360 HLKVFAKPKI TYVENKTAME LEDQITLTCE ASGDPIPSIT WKTSTRNISN EEKTLDGRIV 370 380 390 400 410 420 VRSHARVSSL TLKEIQYTDA GEYVCTASNT IGQDSQAMYL EVQYAPKLQG PVAVYTWEGN 430 440 450 460 470 480 QVNITCEVFA YPSAVISWFR DGQLLPSSNY SNIKIYNTPS ASYLEVTPDS ENDFGNYNCT 490 500 510 520 530 540 AVNRIGQESS EFILVQADTP SSPSIDRVEP YSSTARVEFD EPEATGGVPI LKYKAEWRAL 550 560 570 580 590 600 GEGEWHSRLY DAKEANVEGT ITISGLKPET TYSVRLSAVN GKGVGEISLP SDFKTQPVRE 610 620 630 640 650 660 PSAPKLEGQM GEDGNSIKVN VIKQDDGGSP IRHYLIKYKA KHSSEWKPEI RLPSGIDHVM 670 680 690 700 710 720 LKSLDWNAEY EVYVIAENQQ GKSKPAHYAF RTSAQPTVIP ASTSPTSGLG TAAIVGILIV 730 740 750 760 770 780 IFVLLLVAVD VTCYFLNKCG LLMCIAVNLC GKSGPGAKGK DMEEGKAAFS KDESKEPIVE 790 800 810 820 830 840 VRTEEERTPN HDGGKHTEPN ETTPLTEPEH TADTAATVED MLPSVTTGTT NSDTITETFA 850 860 870 880 890 900 TAQNSPTSET TTLTSSIAPP ATAIPDSNAM SPGQATPAKA GASPVSPPPP SSTPKVAPLV 910 920 930 940 950 960 DLSDTPSSAP ATNNLSSSVL SNQGAVLSPS TVANMAETSK AAAGNKSAAP TPANLTSPPA 970 980 990 1000 1010 1020 PSEPKQEVSS TKSPEKEAAQ PSTVKSPTET AKNPSNPKSE AASGGTTNPS QNEDFKMDEG 1030 1040 1050 1060 1070 1080 TFKTPDIDLA KDVFAALGTT TPASVASGQA RELASSTADS SVPAAPAKTE KTPVEDKSEV 1090 QATEIRHLQQ K
	Isoform B (N-CAM 140) [UniParc]. Checksum: 9177244881747480	FASTA	1,052	112,977
	10 20 30 40 50 60 MLPAAALPWT LFFLGAAASL QVDIVPSQGE ISVGESKFFL CQVAGEAKYK DISWFSPNGE 70 80 90 100 110 120 KLTPNQQRIS VVRNDDFSST LTIYNANIDD AGIYKCVVSS VEEGDSEATV NVKIFQKLMF 130 140 150 160 170 180 KNAPTPQEFK EGDDAVIVCD VVSSLPPTII WKHKGRDVML KKDVRFIVLS NNYLQIRGIK 190 200 210 220 230 240 KTDEGTYRCE GRILARGEIN FKDIQVIVNV PPSVRARQST MNATANLSQS VTLACDADGF 250 260 270 280 290 300 PEPTMTWTKD GEPIEQEDNE EKYSFNYDGS ELIIKKVDKS DEAEYICIAE NKAGEQDATI 310 320 330 340 350 360 HLKVFAKPKI TYVENKTAME LEDQITLTCE ASGDPIPSIT WKTSTRNISN EEKTLDGRIV 370 380 390 400 410 420 VRSHARVSSL TLKEIQYTDA GEYVCTASNT IGQDSQAMYL EVQYAPKLQG PVAVYTWEGN 430 440 450 460 470 480 QVNITCEVFA YPSAVISWFR DGQLLPSSNY SNIKIYNTPS ASYLEVTPDS ENDFGNYNCT 490 500 510 520 530 540 AVNRIGQESS EFILVQADTP SSPSIDRVEP YSSTARVEFD EPEATGGVPI LKYKAEWRAL 550 560 570 580 590 600 GEGEWHSRLY DAKEANVEGT ITISGLKPET TYSVRLSAVN GKGVGEISLP SDFKTQPVRE 610 620 630 640 650 660 PSAPKLEGQM GEDGNSIKVN VIKQDDGGSP IRHYLIKYKA KHSSEWKPEI RLPSGIDHVM 670 680 690 700 710 720 LKSLDWNAEY EVYVIAENQQ GKSKPAHYAF RTSAQPTVIP ASTSPTSGLG TAAIVGILIV 730 740 750 760 770 780 IFVLLLVAVD VTCYFLNKCG LLMCIAVNLC GKSGPGAKGK DMEEGKAAFS HTADTAATVE 790 800 810 820 830 840 DMLPSVTTGT TNSDTITETF ATAQNSPTSE TTTLTSSIAP PATAIPDSNA MSPGQATPAK 850 860 870 880 890 900 AGASPVSPPP PSSTPKVAPL VDLSDTPSSA PATNNLSSSV LSNQGAVLSP STVANMAETS 910 920 930 940 950 960 KAAAGNKSAA PTPANLTSPP APSEPKQEVS STKSPEKEAA QPSTVKSPTE TAKNPSNPKS 970 980 990 1000 1010 1020 EAASGGTTNP SQNEDFKMDE GTFKTPDIDL AKDVFAALGT TTPASVASGQ ARELASSTAD 1030 1040 1050 SSVPAAPAKT EKTPVEDKSE VQATEIRHLQ QK
	Isoform C [UniParc]. Checksum: A07527ADC5CCCBD1	FASTA	830	91,573
	10 20 30 40 50 60 MLPAAALPWT LFFLGAAASL QVDIVPSQGE ISVGESKFFL CQVAGEAKYK DISWFSPNGE 70 80 90 100 110 120 KLTPNQQRIS VVRNDDFSST LTIYNANIDD AGIYKCVVSS VEEGDSEATV NVKIFQKLMF 130 140 150 160 170 180 KNAPTPQEFK EGDDAVIVCD VVSSLPPTII WKHKGRDVML KKDVRFIVLS NNYLQIRGIK 190 200 210 220 230 240 KTDEGTYRCE GRILARGEIN FKDIQVIVNV PPSVRARQST MNATANLSQS VTLACDADGF 250 260 270 280 290 300 PEPTMTWTKD GEPIEQEDNE EKYSFNYDGS ELIIKKVDKS DEAEYICIAE NKAGEQDATI 310 320 330 340 350 360 HLKVFAKPKI TYVENKTAME LEDQITLTCE ASGDPIPSIT WKTSTRNISN EEKTLDGRIV 370 380 390 400 410 420 VRSHARVSSL TLKEIQYTDA GEYVCTASNT IGQDSQAMYL EVQYAPKLQG PVAVYTWEGN 430 440 450 460 470 480 QVNITCEVFA YPSAVISWFR DGQLLPSSNY SNIKIYNTPS ASYLEVTPDS ENDFGNYNCT 490 500 510 520 530 540 AVNRIGQESS EFILVQADTP SSPSIDRVEP YSSTARVEFD EPEATGGVPI LKYKAEWRAL 550 560 570 580 590 600 GEGEWHSRLY DAKEANVEGT ITISGLKPET TYSVRLSAVN GKGVGEISLP SDFKTQPVRE 610 620 630 640 650 660 PSAPKLEGQM GEDGNSIKVN VIKQDDGGSP IRHYLIKYKA KHSSEWKPEI RLPSGIDHVM 670 680 690 700 710 720 LKSLDWNAEY EVYVIAENQQ GKSKPAHYAF RTSAQPTVIP ASTSPTSGLG TAAIVGILIV 730 740 750 760 770 780 IFVLLLVAVD VTCYFLNKCG LLMCIAVNLC GKSGPGAKGK DMEEGKAAFS KDESKEPIVE 790 800 810 820 830 VRTEEERTPN HDGGKHTEPN ETTPLTEPEK TPVEDKSEVQ ATEIRHLQQK
	Isoform D [UniParc]. Checksum: E08171FD649020C5	FASTA	726	80,219
	10 20 30 40 50 60 MLPAAALPWT LFFLGAAASL QVDIVPSQGE ISVGESKFFL CQVAGEAKYK DISWFSPNGE 70 80 90 100 110 120 KLTPNQQRIS VVRNDDFSST LTIYNANIDD AGIYKCVVSS VEEGDSEATV NVKIFQKLMF 130 140 150 160 170 180 KNAPTPQEFK EGDDAVIVCD VVSSLPPTII WKHKGRDVML KKDVRFIVLS NNYLQIRGIK 190 200 210 220 230 240 KTDEGTYRCE GRILARGEIN FKDIQVIVNV PPSVRARQST MNATANLSQS VTLACDADGF 250 260 270 280 290 300 PEPTMTWTKD GEPIEQEDNE EKYSFNYDGS ELIIKKVDKS DEAEYICIAE NKAGEQDATI 310 320 330 340 350 360 HLKVFAKPKI TYVENKTAME LEDQITLTCE ASGDPIPSIT WKTSTRNISN EEKTLDGRIV 370 380 390 400 410 420 VRSHARVSSL TLKEIQYTDA GEYVCTASNT IGQDSQAMYL EVQYAPKLQG PVAVYTWEGN 430 440 450 460 470 480 QVNITCEVFA YPSAVISWFR DGQLLPSSNY SNIKIYNTPS ASYLEVTPDS ENDFGNYNCT 490 500 510 520 530 540 AVNRIGQESS EFILVQADTP SSPSIDRVEP YSSTARVEFD EPEATGGVPI LKYKAEWRAL 550 560 570 580 590 600 GEGEWHSRLY DAKEANVEGT ITISGLKPET TYSVRLSAVN GKGVGEISLP SDFKTQPVRE 610 620 630 640 650 660 PSAPKLEGQM GEDGNSIKVN VIKQDDGGSP IRHYLIKYKA KHSSEWKPEI RLPSGIDHVM 670 680 690 700 710 720 LKSLDWNAEY EVYVIAENQQ GKSKPAHYAF RTSAQPTVIP ATLGSPSTSS SFVSLLLSAV TLLLLC

References

[1]	"Neural cell adhesion molecule: structure, immunoglobulin-like domains, cell surface modulation, and alternative RNA splicing." Cunningham B.A., Hemperly J.J., Murray B.A., Prediger E.A., Brackenbury R., Edelman G.M. Science 236:799-806(1987) [PubMed: 3576199] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-175, PARTIAL PROTEIN SEQUENCE.
[2]	"Sequence of a cDNA clone encoding the polysialic acid-rich and cytoplasmic domains of the neural cell adhesion molecule N-CAM." Hemperly J.J., Murray B.A., Edelman G.M., Cunningham B.A. Proc. Natl. Acad. Sci. U.S.A. 83:3037-3041(1986) [PubMed: 3458261] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 128-1091, PARTIAL PROTEIN SEQUENCE.
[3]	"cDNA clones of the neural cell adhesion molecule (N-CAM) lacking a membrane-spanning region consistent with evidence for membrane attachment via a phosphatidylinositol intermediate." Hemperly J.J., Edelman G.M., Cunningham B.A. Proc. Natl. Acad. Sci. U.S.A. 83:9822-9826(1986) [PubMed: 3467341] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM D).
[4]	"Cell surface modulation of the neural cell adhesion molecule resulting from alternative mRNA splicing in a tissue-specific developmental sequence." Murray B.A., Owens G.C., Prediger E.A., Crossin K.L., Cunningham B.A., Edelman G.M. J. Cell Biol. 103:1431-1439(1986) [PubMed: 3771645] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 810-1069.
[5]	"Conserved regulatory elements in the promoter region of the N-CAM gene." Colwell G., Li B., Forrest D., Brackenbury R. Genomics 14:875-882(1992) [PubMed: 1478668] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-17.
[6]	Sasner M., Covault J. Submitted (AUG-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-17. Strain: White leghorn. Tissue: Erythrocyte.
[7]	"Topographic localization of the heparin-binding domain of the neural cell adhesion molecule N-CAM." Cole G.J., Loewy A., Cross N.V., Akeson R., Glaser L. J. Cell Biol. 103:1739-1744(1986) [PubMed: 2430978] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 20-29.
[8]	"Identification of a peptide sequence involved in homophilic binding in the neural cell adhesion molecule NCAM." Rao Y., Wu X.F., Gariepy J., Rutishauser U., Siu C.H. J. Cell Biol. 118:937-949(1992) [PubMed: 1380002] [Abstract] Cited for: CELL-BINDING.
[9]	"Solution structure of the third immunoglobulin domain of the neural cell adhesion molecule N-CAM: can solution studies define the mechanism of homophilic binding?" Atkins A.R., Chung J., Deechongkit S., Little E.B., Edelman G.M., Wright P.E., Cunningham B.A., Dyson H.J. J. Mol. Biol. 311:161-172(2001) [PubMed: 11469865] [Abstract] Cited for: STRUCTURE BY NMR OF 202-307.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M15861 , M15860, M15922, M15923, M15924, M21178, M21179, M21180, M15929, M15930, M15931, M15932, M15933, M15934, L29437, M15935, M15937, M15938, M15939 Genomic DNA. Translation: AAB59958.1.
M15861 , M15860, M15922, M15923, M15924, M21178, M21179, M21180, M15929, M15930, M15931, M15932, M15934, L29437, M15935, M15937, M15939 Genomic DNA. Translation: AAB59959.1.
M15936 , M15860, M15922, M15923, M15924, M21178, M21179, M21180, M15929, M15930, M15931, M15932, M15934, L29437, M15935 Genomic DNA. Translation: AAB59957.1.
X04479 Genomic DNA. Translation: CAB51638.1.
X70342 Genomic DNA. Translation: CAA49807.1.
Z12128 Genomic DNA. Translation: CAA78113.1.

IPI

IPI00591580.
IPI00591739.
IPI00595516.
IPI00680755.

PIR

IJCHNL. A43613.

RefSeq

NP_001122300.1. NM_001128828.1.

UniGene

Gga.47595.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1IE5	NMR	-	A	202-307	[»]

ProteinModelPortal

P13590.

SMR

P13590. Positions 20-307, 496-693.

ModBase

Search...

Protein-protein interaction databases

IntAct

P13590. 1 interaction.

STRING

P13590.

Proteomic databases

PRIDE

P13590.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

428253.

KEGG

gga:428253.

Organism-specific databases

CTD

255239.

Phylogenomic databases

eggNOG

veNOG05507.

GeneTree

ENSGT00600000084036.

HOGENOM

HBG444274.

HOVERGEN

HBG052579.

InParanoid

P13590.

OrthoDB

EOG40VVP1.

Enzyme and pathway databases

Reactome

REACT_115433. Developmental Biology.

Family and domain databases

InterPro

IPR003961. Fibronectin_type3.
IPR007110. Ig-like.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR009138. Neural_cell_adh.
[Graphical view]

Gene3D

G3DSA:2.60.40.10. Ig-like_fold. 7 hits.

KO

K06491.

Pfam

PF00041. fn3. 2 hits.
PF07679. I-set. 5 hits.
[Graphical view]

PRINTS

PR01838. NCAMFAMILY.

SMART

SM00060. FN3. 2 hits.
SM00409. IG. 1 hit.
SM00408. IGc2. 4 hits.
[Graphical view]

SUPFAM

SSF49265. FN_III-like. 2 hits.

PROSITE

PS50853. FN3. 2 hits.
PS50835. IG_LIKE. 5 hits.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

NCAM1_CHICK

Accession

Primary (citable) accession number: P13590
Secondary accession number(s): Q90918, Q90919

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	July 15, 1998
Last modified:	November 16, 2011
This is version 113 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program