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Protein

Pituitary adenylate cyclase-activating polypeptide

Gene

Adcyap1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binding to its receptor activates G proteins and stimulates adenylate cyclase in pituitary cells (By similarity). Promotes neuron projection development through the RAPGEF2/Rap1/B-Raf/ERK pathway. In chromaffin cells, induces long-lasting increase of intracellular calcium concentrations and neuroendocrine secretion (PubMed:18198219). Involved in the control of glucose homeostasis, induces insulin secretion by pancreatic beta cells (By similarity).By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

  • activation of adenylate cyclase activity Source: Reactome
  • ATP metabolic process Source: RGD
  • behavioral fear response Source: RGD
  • cAMP-mediated signaling Source: UniProtKB
  • cellular response to glucocorticoid stimulus Source: RGD
  • histamine secretion Source: RGD
  • insulin secretion Source: UniProtKB
  • negative regulation of acute inflammatory response to antigenic stimulus Source: RGD
  • negative regulation of acute inflammatory response to non-antigenic stimulus Source: RGD
  • negative regulation of cell cycle Source: RGD
  • negative regulation of glial cell proliferation Source: RGD
  • negative regulation of GTPase activity Source: RGD
  • negative regulation of muscle cell apoptotic process Source: RGD
  • negative regulation of potassium ion transport Source: RGD
  • neuron projection development Source: UniProtKB
  • neuropeptide signaling pathway Source: UniProtKB
  • ovarian follicle development Source: RGD
  • pituitary gland development Source: RGD
  • positive regulation of adenylate cyclase activity Source: ParkinsonsUK-UCL
  • positive regulation of adenylate cyclase activity involved in G-protein coupled receptor signaling pathway Source: BHF-UCL
  • positive regulation of blood vessel diameter Source: RGD
  • positive regulation of cAMP biosynthetic process Source: RGD
  • positive regulation of cell proliferation Source: RGD
  • positive regulation of chemokine (C-C motif) ligand 5 production Source: RGD
  • positive regulation of cytosolic calcium ion concentration Source: RGD
  • positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
  • positive regulation of gene expression Source: RGD
  • positive regulation of growth hormone secretion Source: RGD
  • positive regulation of GTPase activity Source: UniProtKB
  • positive regulation of interleukin-6 production Source: RGD
  • positive regulation of neuron projection development Source: RGD
  • positive regulation of protein kinase activity Source: UniProtKB
  • positive regulation of somatostatin secretion Source: RGD
  • positive regulation of synaptic transmission, glutamatergic Source: RGD
  • positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • regulation of G-protein coupled receptor protein signaling pathway Source: UniProtKB
  • regulation of oligodendrocyte progenitor proliferation Source: RGD
  • regulation of postsynaptic membrane potential Source: RGD
  • regulation of protein localization Source: BHF-UCL
  • regulation of protein phosphorylation Source: ParkinsonsUK-UCL
  • response to ethanol Source: RGD
  • response to starvation Source: RGD
  • sensory perception of pain Source: RGD

Keywordsi

Molecular functionHormone
Biological processNeurogenesis

Enzyme and pathway databases

ReactomeiR-RNO-418555. G alpha (s) signalling events.
R-RNO-420092. Glucagon-type ligand receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Pituitary adenylate cyclase-activating polypeptide
Short name:
PACAP
Cleaved into the following 3 chains:
Alternative name(s):
PRP-48
Pituitary adenylate cyclase-activating polypeptide 27
Short name:
PACAP-27
Short name:
PACAP27
Pituitary adenylate cyclase-activating polypeptide 38
Short name:
PACAP-38
Short name:
PACAP38
Gene namesi
Name:Adcyap1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 9

Organism-specific databases

RGDi2037. Adcyap1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
PropeptideiPRO_000001150125 – 78Add BLAST54
PeptideiPRO_000001150281 – 128PACAP-related peptideAdd BLAST48
PeptideiPRO_0000011503131 – 168Pituitary adenylate cyclase-activating polypeptide 38Add BLAST38
PeptideiPRO_0000011504131 – 157Pituitary adenylate cyclase-activating polypeptide 27Add BLAST27
PropeptideiPRO_0000011505172 – 1754

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei157Leucine amideBy similarity1
Modified residuei168Lysine amide1 Publication1

Keywords - PTMi

Amidation, Cleavage on pair of basic residues

Proteomic databases

PaxDbiP13589.

Expressioni

Gene expression databases

BgeeiENSRNOG00000049882.
ExpressionAtlasiP13589. baseline and differential.
GenevisibleiP13589. RN.

Interactioni

Subunit structurei

Interacts with ADCYAP1R1 (via N-terminal extracellular domain).By similarity

GO - Molecular functioni

  • neuropeptide hormone activity Source: UniProtKB
  • peptide hormone receptor binding Source: UniProtKB
  • pituitary adenylate cyclase activating polypeptide activity Source: BHF-UCL
  • pituitary adenylate cyclase-activating polypeptide receptor binding Source: RGD
  • receptor binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000063920.

Chemistry databases

BindingDBiP13589.

Structurei

3D structure databases

ProteinModelPortaliP13589.
SMRiP13589.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni149 – 157Important for receptor bindingBy similarity9

Sequence similaritiesi

Belongs to the glucagon family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IWIZ. Eukaryota.
ENOG4111FZG. LUCA.
GeneTreeiENSGT00530000063592.
HOVERGENiHBG018069.
InParanoidiP13589.
KOiK05262.
OMAiRYRQRIR.
PhylomeDBiP13589.

Family and domain databases

InterProiView protein in InterPro
IPR000532. Glucagon_GIP_secretin_VIP.
PfamiView protein in Pfam
PF00123. Hormone_2. 2 hits.
PRINTSiPR00275. GLUCAGON.
SMARTiView protein in SMART
SM00070. GLUCA. 2 hits.
PROSITEiView protein in PROSITE
PS00260. GLUCAGON. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13589-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTMCSGARLA LLVYGIIMHN SVSCSPAAGL SFPGIRPEEE AYDQDGNPLQ
60 70 80 90 100
DFYDWDPPGA GSPASALRDA YALYYPADRR DVAHEILNEA YRKVLDQLSA
110 120 130 140 150
RKYLQSMVAR GMGENLAAAA VDDRAPLTKR HSDGIFTDSY SRYRKQMAVK
160 170
KYLAAVLGKR YKQRVKNKGR RIAYL
Length:175
Mass (Da):19,557
Last modified:March 1, 1992 - v2
Checksum:i0398946896602B04
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7A → R in CAA56564 (PubMed:7835287).Curated1
Sequence conflicti26P → L in CAA56564 (PubMed:7835287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63006 mRNA. Translation: AAA41791.1.
X80290 mRNA. Translation: CAA56564.1.
PIRiA37786.
RefSeqiNP_058685.1. NM_016989.2.
XP_006245734.1. XM_006245672.3.
XP_006245735.1. XM_006245673.3.
XP_006245736.1. XM_006245674.3.
XP_008765640.1. XM_008767418.2.
UniGeneiRn.202559.
Rn.37400.

Genome annotation databases

EnsembliENSRNOT00000087405; ENSRNOP00000070007; ENSRNOG00000049882.
GeneIDi24166.
KEGGirno:24166.

Similar proteinsi

Entry informationi

Entry nameiPACA_RAT
AccessioniPrimary (citable) accession number: P13589
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: March 1, 1992
Last modified: November 22, 2017
This is version 119 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families