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P13587

- ATN1_YEAST

UniProt

P13587 - ATN1_YEAST

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Protein

Sodium transport ATPase 1

Gene

ENA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the sodium or lithium ions to allow salt tolerance. Is negatively modulated by SIS2/HAL3.2 Publications

Catalytic activityi

ATP + H2O + Na+(In) = ADP + phosphate + Na+(Out).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei369 – 36914-aspartylphosphate intermediateBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: InterPro
  3. potassium-transporting ATPase activity Source: SGD
  4. sodium-exporting ATPase activity, phosphorylative mechanism Source: SGD

GO - Biological processi

  1. cellular response to glucose starvation Source: SGD
  2. hyperosmotic response Source: SGD
  3. potassium ion transmembrane transport Source: GOC
  4. potassium ion transport Source: SGD
  5. response to pH Source: SGD
  6. response to salt stress Source: UniProtKB
  7. sodium ion transmembrane transport Source: GOC
  8. sodium ion transport Source: SGD
  9. transmembrane transport Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Ion transport, Sodium transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding, Sodium

Enzyme and pathway databases

BioCyciYEAST:G3O-29654-MONOMER.

Protein family/group databases

TCDBi3.A.3.9.1. the p-type atpase (p-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium transport ATPase 1 (EC:3.6.3.7)
Gene namesi
Name:ENA1
Synonyms:HOR6, PMR2, PMR2A
Ordered Locus Names:YDR040C
ORF Names:YD6888.02C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

CYGDiYDR040c.
SGDiS000002447. ENA1.

Subcellular locationi

Cell membrane 3 Publications; Multi-pass membrane protein 3 Publications

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10911091Sodium transport ATPase 1PRO_0000046242Add
BLAST

Proteomic databases

MaxQBiP13587.
PeptideAtlasiP13587.

Expressioni

Inductioni

By sodium ions. Induction at low salt concentrations (0.3 M) is mediated by the high-osmolarity glycerol (HOG)-MAP kinase pathway, a system activated by non-specific osmotic stress, and by the protein kinase A pathway. At high salt concentrations (0.8 M) is mediated by the protein phosphatase calcineurin, which is specifically activated by sodium ions.2 Publications

Gene expression databases

GenevestigatoriP13587.

Interactioni

Protein-protein interaction databases

BioGridi32095. 35 interactions.
DIPiDIP-4493N.
MINTiMINT-524960.
STRINGi4932.YDR040C.

Structurei

3D structure databases

ProteinModelPortaliP13587.
SMRiP13587. Positions 16-1006.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6363CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini85 – 906ExtracellularSequence Analysis
Topological domaini112 – 282171CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini304 – 3129ExtracellularSequence Analysis
Topological domaini334 – 815482CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini837 – 84812ExtracellularSequence AnalysisAdd
BLAST
Topological domaini870 – 88516CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini907 – 94337ExtracellularSequence AnalysisAdd
BLAST
Topological domaini965 – 99127CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini1013 – 10219ExtracellularSequence Analysis
Topological domaini1043 – 109149CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei64 – 8421HelicalSequence AnalysisAdd
BLAST
Transmembranei91 – 11121HelicalSequence AnalysisAdd
BLAST
Transmembranei283 – 30321HelicalSequence AnalysisAdd
BLAST
Transmembranei313 – 33321HelicalSequence AnalysisAdd
BLAST
Transmembranei816 – 83621HelicalSequence AnalysisAdd
BLAST
Transmembranei849 – 86921HelicalSequence AnalysisAdd
BLAST
Transmembranei886 – 90621HelicalSequence AnalysisAdd
BLAST
Transmembranei944 – 96421HelicalSequence AnalysisAdd
BLAST
Transmembranei992 – 101221HelicalSequence AnalysisAdd
BLAST
Transmembranei1022 – 104221HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00760000119767.
HOGENOMiHOG000265621.
InParanoidiP13587.
OMAiYENESCE.
OrthoDBiEOG74BK1C.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 2 hits.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR006414. ATPase_P-typ_Na/Ca.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 2 hits.
TIGRFAMsiTIGR01523. ATPase-IID_K-Na. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P13587-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGEGTTKENN NAEFNAYHTL TAEEAAEFIG TSLTEGLTQD EFVHRLKTVG
60 70 80 90 100
ENTLGDDTKI DYKAMVLHQV CNAMIMVLLI SMIISFAMHD WITGGVISFV
110 120 130 140 150
IAVNVLIGLV QEYKATKTMN SLKNLSSPNA HVIRNGKSET INSKDVVPGD
160 170 180 190 200
ICLVKVGDTI PADLRLIETK NFDTDESLLT GESLPVSKDA NLVFGKEEET
210 220 230 240 250
SVGDRLNLAF SSSAVVKGRA KGIVIKTALN SEIGKIAKSL QGDSGLISRD
260 270 280 290 300
PSKSWLQNTW ISTKKVTGAF LGTNVGTPLH RKLSKLAVLL FWIAVLFAII
310 320 330 340 350
VMASQKFDVD KRVAIYAICV ALSMIPSSLV VVLTITMSVG AAVMVSRNVI
360 370 380 390 400
VRKLDSLEAL GAVNDICSDK TGTLTQGKML ARQIWIPRFG TITISNSDDP
410 420 430 440 450
FNPNEGNVSL IPRFSPYEYS HNEDGDVGIL QNFKDRLYEK DLPEDIDMDL
460 470 480 490 500
FQKWLETATL ANIATVFKDD ATDCWKAHGD PTEIAIQVFA TKMDLPHNAL
510 520 530 540 550
TGEKSTNQSN ENDQSSLSQH NEKPGSAQFE HIAEFPFDST VKRMSSVYYN
560 570 580 590 600
NHNETYNIYG KGAFESIISC CSSWYGKDGV KITPLTDCDV ETIRKNVYSL
610 620 630 640 650
SNEGLRVLGF ASKSFTKDQV NDDQLKNITS NRATAESDLV FLGLIGIYDP
660 670 680 690 700
PRNETAGAVK KFHQAGINVH MLTGDFVGTA KAIAQEVGIL PTNLYHYSQE
710 720 730 740 750
IVDSMVMTGS QFDGLSEEEV DDLPVLPLVI ARCSPQTKVR MIEALHRRKK
760 770 780 790 800
FCTMTGDGVN DSPSLKMANV GIAMGINGSD VSKEASDIVL SDDNFASILN
810 820 830 840 850
AVEEGRRMTD NIQKFVLQLL AENVAQALYL IIGLVFRDEN GKSVFPLSPV
860 870 880 890 900
EVLWIIVVTS CFPAMGLGLE KAAPDLMDRP PHDSEVGIFT WEVIIDTFAY
910 920 930 940 950
GIIMTGSCMA SFTGSLYGIN SGRLGHDCDG TYNSSCRDVY RSRSAAFATM
960 970 980 990 1000
TWCALILAWE VVDMRRSFFR MHPDTDSPVK EFFRSIWGNQ FLFWSIIFGF
1010 1020 1030 1040 1050
VSAFPVVYIP VINDKVFLHK PIGAEWGLAI AFTIAFWIGA ELYKCGKRRY
1060 1070 1080 1090
FKTQRAHNPE NDLESNNKRD PFEAYSTSTT IHTEVNIGIK Q
Length:1,091
Mass (Da):120,357
Last modified:January 1, 1990 - v1
Checksum:i6480DBCD92B555E6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U24069 Genomic DNA. Translation: AAA65600.1.
Z54075 Genomic DNA. Translation: CAA90779.1.
Z74336 Genomic DNA. Translation: CAA98867.1.
X58626 Genomic DNA. Translation: CAA41479.1.
J04186 Genomic DNA. No translation available.
BK006938 Genomic DNA. Translation: DAA11888.1.
PIRiS05788. PWBYR2.
RefSeqiNP_010325.1. NM_001180348.1.

Genome annotation databases

EnsemblFungiiYDR040C; YDR040C; YDR040C.
GeneIDi851610.
KEGGisce:YDR040C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U24069 Genomic DNA. Translation: AAA65600.1 .
Z54075 Genomic DNA. Translation: CAA90779.1 .
Z74336 Genomic DNA. Translation: CAA98867.1 .
X58626 Genomic DNA. Translation: CAA41479.1 .
J04186 Genomic DNA. No translation available.
BK006938 Genomic DNA. Translation: DAA11888.1 .
PIRi S05788. PWBYR2.
RefSeqi NP_010325.1. NM_001180348.1.

3D structure databases

ProteinModelPortali P13587.
SMRi P13587. Positions 16-1006.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32095. 35 interactions.
DIPi DIP-4493N.
MINTi MINT-524960.
STRINGi 4932.YDR040C.

Protein family/group databases

TCDBi 3.A.3.9.1. the p-type atpase (p-atpase) superfamily.

Proteomic databases

MaxQBi P13587.
PeptideAtlasi P13587.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDR040C ; YDR040C ; YDR040C .
GeneIDi 851610.
KEGGi sce:YDR040C.

Organism-specific databases

CYGDi YDR040c.
SGDi S000002447. ENA1.

Phylogenomic databases

GeneTreei ENSGT00760000119767.
HOGENOMi HOG000265621.
InParanoidi P13587.
OMAi YENESCE.
OrthoDBi EOG74BK1C.

Enzyme and pathway databases

BioCyci YEAST:G3O-29654-MONOMER.

Miscellaneous databases

NextBioi 969124.

Gene expression databases

Genevestigatori P13587.

Family and domain databases

Gene3Di 1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 2 hits.
InterProi IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR006414. ATPase_P-typ_Na/Ca.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view ]
Pfami PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view ]
PRINTSi PR00119. CATATPASE.
SMARTi SM00831. Cation_ATPase_N. 1 hit.
[Graphical view ]
SUPFAMi SSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 2 hits.
TIGRFAMsi TIGR01523. ATPase-IID_K-Na. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The yeast secretory pathway is perturbed by mutations in PMR1, a member of a Ca2+ ATPase family."
    Rudolph H.K., Antebi A., Fink G.R., Buckley C.M., Dorman T.E., Levitre J., Davidow L.S., Mao J.-I., Moir D.T.
    Cell 58:133-145(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The PMR2 gene cluster encodes functionally distinct isoforms of a putative Na+ pump in the yeast plasma membrane."
    Wieland J., Nitsche A.M., Strayle J., Steiner H., Rudolph H.K.
    EMBO J. 14:3870-3882(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, INDUCTION.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "A PMR2 tandem repeat with a modified C-terminus is located downstream from the KRS1 gene encoding lysyl-tRNA synthetase in Saccharomyces cerevisiae."
    Martinez R., Latreille M.-T., Mirande M.
    Mol. Gen. Genet. 227:149-154(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 534-1091.
    Strain: 7305B.
  6. "The yeast lysyl-tRNA synthetase gene. Evidence for general amino acid control of its expression and domain structure of the encoded protein."
    Mirande M., Waller J.-P.
    J. Biol. Chem. 263:18443-18451(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 534-1091.
    Strain: ATCC 26109 / X2180.
  7. "Multiple transduction pathways regulate the sodium-extrusion gene PMR2/ENA1 during salt stress in yeast."
    Marquez J.A., Serrano R.
    FEBS Lett. 382:89-92(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  8. "Overexpression of the sodium ATPase of Saccharomyces cerevisiae: conditions for phosphorylation from ATP and Pi."
    Benito B., Quintero F.J., Rodriguez-Navarro A.
    Biochim. Biophys. Acta 1328:214-226(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  11. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.

Entry informationi

Entry nameiATN1_YEAST
AccessioniPrimary (citable) accession number: P13587
Secondary accession number(s): D6VS28
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: October 29, 2014
This is version 151 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 688 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3