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P13587

- ATN1_YEAST

UniProt

P13587 - ATN1_YEAST

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Protein
Sodium transport ATPase 1
Gene
ENA1, HOR6, PMR2, PMR2A, YDR040C, YD6888.02C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the sodium or lithium ions to allow salt tolerance. Is negatively modulated by SIS2/HAL3.2 Publications

Catalytic activityi

ATP + H2O + Na+(In) = ADP + phosphate + Na+(Out).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei369 – 36914-aspartylphosphate intermediate By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: InterPro
  3. potassium-transporting ATPase activity Source: SGD
  4. sodium-exporting ATPase activity, phosphorylative mechanism Source: SGD
Complete GO annotation...

GO - Biological processi

  1. cellular response to glucose starvation Source: SGD
  2. hyperosmotic response Source: SGD
  3. potassium ion transmembrane transport Source: GOC
  4. potassium ion transport Source: SGD
  5. response to pH Source: SGD
  6. response to salt stress Source: UniProtKB
  7. sodium ion transmembrane transport Source: GOC
  8. sodium ion transport Source: SGD
  9. transmembrane transport Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Ion transport, Sodium transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding, Sodium

Enzyme and pathway databases

BioCyciYEAST:G3O-29654-MONOMER.

Protein family/group databases

TCDBi3.A.3.9.1. the p-type atpase (p-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium transport ATPase 1 (EC:3.6.3.7)
Gene namesi
Name:ENA1
Synonyms:HOR6, PMR2, PMR2A
Ordered Locus Names:YDR040C
ORF Names:YD6888.02C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

CYGDiYDR040c.
SGDiS000002447. ENA1.

Subcellular locationi

Cell membrane; Multi-pass membrane protein 3 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6363Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei64 – 8421Helical; Reviewed prediction
Add
BLAST
Topological domaini85 – 906Extracellular Reviewed prediction
Transmembranei91 – 11121Helical; Reviewed prediction
Add
BLAST
Topological domaini112 – 282171Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei283 – 30321Helical; Reviewed prediction
Add
BLAST
Topological domaini304 – 3129Extracellular Reviewed prediction
Transmembranei313 – 33321Helical; Reviewed prediction
Add
BLAST
Topological domaini334 – 815482Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei816 – 83621Helical; Reviewed prediction
Add
BLAST
Topological domaini837 – 84812Extracellular Reviewed prediction
Add
BLAST
Transmembranei849 – 86921Helical; Reviewed prediction
Add
BLAST
Topological domaini870 – 88516Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei886 – 90621Helical; Reviewed prediction
Add
BLAST
Topological domaini907 – 94337Extracellular Reviewed prediction
Add
BLAST
Transmembranei944 – 96421Helical; Reviewed prediction
Add
BLAST
Topological domaini965 – 99127Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei992 – 101221Helical; Reviewed prediction
Add
BLAST
Topological domaini1013 – 10219Extracellular Reviewed prediction
Transmembranei1022 – 104221Helical; Reviewed prediction
Add
BLAST
Topological domaini1043 – 109149Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10911091Sodium transport ATPase 1
PRO_0000046242Add
BLAST

Proteomic databases

MaxQBiP13587.
PeptideAtlasiP13587.

Expressioni

Inductioni

By sodium ions. Induction at low salt concentrations (0.3 M) is mediated by the high-osmolarity glycerol (HOG)-MAP kinase pathway, a system activated by non-specific osmotic stress, and by the protein kinase A pathway. At high salt concentrations (0.8 M) is mediated by the protein phosphatase calcineurin, which is specifically activated by sodium ions.2 Publications

Gene expression databases

GenevestigatoriP13587.

Interactioni

Protein-protein interaction databases

BioGridi32095. 35 interactions.
DIPiDIP-4493N.
MINTiMINT-524960.
STRINGi4932.YDR040C.

Structurei

3D structure databases

ProteinModelPortaliP13587.
SMRiP13587. Positions 16-1006.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00560000076866.
HOGENOMiHOG000265621.
OMAiYENESCE.
OrthoDBiEOG74BK1C.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 2 hits.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR006414. ATPase_P-typ_Na/Ca.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 2 hits.
TIGRFAMsiTIGR01523. ATPase-IID_K-Na. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P13587-1 [UniParc]FASTAAdd to Basket

« Hide

MGEGTTKENN NAEFNAYHTL TAEEAAEFIG TSLTEGLTQD EFVHRLKTVG     50
ENTLGDDTKI DYKAMVLHQV CNAMIMVLLI SMIISFAMHD WITGGVISFV 100
IAVNVLIGLV QEYKATKTMN SLKNLSSPNA HVIRNGKSET INSKDVVPGD 150
ICLVKVGDTI PADLRLIETK NFDTDESLLT GESLPVSKDA NLVFGKEEET 200
SVGDRLNLAF SSSAVVKGRA KGIVIKTALN SEIGKIAKSL QGDSGLISRD 250
PSKSWLQNTW ISTKKVTGAF LGTNVGTPLH RKLSKLAVLL FWIAVLFAII 300
VMASQKFDVD KRVAIYAICV ALSMIPSSLV VVLTITMSVG AAVMVSRNVI 350
VRKLDSLEAL GAVNDICSDK TGTLTQGKML ARQIWIPRFG TITISNSDDP 400
FNPNEGNVSL IPRFSPYEYS HNEDGDVGIL QNFKDRLYEK DLPEDIDMDL 450
FQKWLETATL ANIATVFKDD ATDCWKAHGD PTEIAIQVFA TKMDLPHNAL 500
TGEKSTNQSN ENDQSSLSQH NEKPGSAQFE HIAEFPFDST VKRMSSVYYN 550
NHNETYNIYG KGAFESIISC CSSWYGKDGV KITPLTDCDV ETIRKNVYSL 600
SNEGLRVLGF ASKSFTKDQV NDDQLKNITS NRATAESDLV FLGLIGIYDP 650
PRNETAGAVK KFHQAGINVH MLTGDFVGTA KAIAQEVGIL PTNLYHYSQE 700
IVDSMVMTGS QFDGLSEEEV DDLPVLPLVI ARCSPQTKVR MIEALHRRKK 750
FCTMTGDGVN DSPSLKMANV GIAMGINGSD VSKEASDIVL SDDNFASILN 800
AVEEGRRMTD NIQKFVLQLL AENVAQALYL IIGLVFRDEN GKSVFPLSPV 850
EVLWIIVVTS CFPAMGLGLE KAAPDLMDRP PHDSEVGIFT WEVIIDTFAY 900
GIIMTGSCMA SFTGSLYGIN SGRLGHDCDG TYNSSCRDVY RSRSAAFATM 950
TWCALILAWE VVDMRRSFFR MHPDTDSPVK EFFRSIWGNQ FLFWSIIFGF 1000
VSAFPVVYIP VINDKVFLHK PIGAEWGLAI AFTIAFWIGA ELYKCGKRRY 1050
FKTQRAHNPE NDLESNNKRD PFEAYSTSTT IHTEVNIGIK Q 1091
Length:1,091
Mass (Da):120,357
Last modified:January 1, 1990 - v1
Checksum:i6480DBCD92B555E6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U24069 Genomic DNA. Translation: AAA65600.1.
Z54075 Genomic DNA. Translation: CAA90779.1.
Z74336 Genomic DNA. Translation: CAA98867.1.
X58626 Genomic DNA. Translation: CAA41479.1.
J04186 Genomic DNA. No translation available.
BK006938 Genomic DNA. Translation: DAA11888.1.
PIRiS05788. PWBYR2.
RefSeqiNP_010325.1. NM_001180348.1.

Genome annotation databases

EnsemblFungiiYDR040C; YDR040C; YDR040C.
GeneIDi851610.
KEGGisce:YDR040C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U24069 Genomic DNA. Translation: AAA65600.1 .
Z54075 Genomic DNA. Translation: CAA90779.1 .
Z74336 Genomic DNA. Translation: CAA98867.1 .
X58626 Genomic DNA. Translation: CAA41479.1 .
J04186 Genomic DNA. No translation available.
BK006938 Genomic DNA. Translation: DAA11888.1 .
PIRi S05788. PWBYR2.
RefSeqi NP_010325.1. NM_001180348.1.

3D structure databases

ProteinModelPortali P13587.
SMRi P13587. Positions 16-1006.
ModBasei Search...

Protein-protein interaction databases

BioGridi 32095. 35 interactions.
DIPi DIP-4493N.
MINTi MINT-524960.
STRINGi 4932.YDR040C.

Protein family/group databases

TCDBi 3.A.3.9.1. the p-type atpase (p-atpase) superfamily.

Proteomic databases

MaxQBi P13587.
PeptideAtlasi P13587.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDR040C ; YDR040C ; YDR040C .
GeneIDi 851610.
KEGGi sce:YDR040C.

Organism-specific databases

CYGDi YDR040c.
SGDi S000002447. ENA1.

Phylogenomic databases

GeneTreei ENSGT00560000076866.
HOGENOMi HOG000265621.
OMAi YENESCE.
OrthoDBi EOG74BK1C.

Enzyme and pathway databases

BioCyci YEAST:G3O-29654-MONOMER.

Miscellaneous databases

NextBioi 969124.

Gene expression databases

Genevestigatori P13587.

Family and domain databases

Gene3Di 1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 2 hits.
InterProi IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR006414. ATPase_P-typ_Na/Ca.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view ]
Pfami PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view ]
PRINTSi PR00119. CATATPASE.
SMARTi SM00831. Cation_ATPase_N. 1 hit.
[Graphical view ]
SUPFAMi SSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 2 hits.
TIGRFAMsi TIGR01523. ATPase-IID_K-Na. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The yeast secretory pathway is perturbed by mutations in PMR1, a member of a Ca2+ ATPase family."
    Rudolph H.K., Antebi A., Fink G.R., Buckley C.M., Dorman T.E., Levitre J., Davidow L.S., Mao J.-I., Moir D.T.
    Cell 58:133-145(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The PMR2 gene cluster encodes functionally distinct isoforms of a putative Na+ pump in the yeast plasma membrane."
    Wieland J., Nitsche A.M., Strayle J., Steiner H., Rudolph H.K.
    EMBO J. 14:3870-3882(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, INDUCTION.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "A PMR2 tandem repeat with a modified C-terminus is located downstream from the KRS1 gene encoding lysyl-tRNA synthetase in Saccharomyces cerevisiae."
    Martinez R., Latreille M.-T., Mirande M.
    Mol. Gen. Genet. 227:149-154(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 534-1091.
    Strain: 7305B.
  6. "The yeast lysyl-tRNA synthetase gene. Evidence for general amino acid control of its expression and domain structure of the encoded protein."
    Mirande M., Waller J.-P.
    J. Biol. Chem. 263:18443-18451(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 534-1091.
    Strain: ATCC 26109 / X2180.
  7. "Multiple transduction pathways regulate the sodium-extrusion gene PMR2/ENA1 during salt stress in yeast."
    Marquez J.A., Serrano R.
    FEBS Lett. 382:89-92(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  8. "Overexpression of the sodium ATPase of Saccharomyces cerevisiae: conditions for phosphorylation from ATP and Pi."
    Benito B., Quintero F.J., Rodriguez-Navarro A.
    Biochim. Biophys. Acta 1328:214-226(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  11. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.

Entry informationi

Entry nameiATN1_YEAST
AccessioniPrimary (citable) accession number: P13587
Secondary accession number(s): D6VS28
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: June 11, 2014
This is version 149 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 688 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

Similar proteinsi