Calcium-transporting ATPase 1 - Saccharomyces cerevisiae (Baker's yeast)

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Reviewed, UniProtKB/Swiss-Prot P13586 (ATC1_YEAST)

Last modified February 9, 2010. Version 107. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Calcium-transporting ATPase 1
    EC=3.6.3.8
Alternative name(s):
    Golgi Ca(2+)-ATPase
    Bypass SOD defects protein 1

Gene names

Name:	PMR1
Synonyms:	BSD1, SCC1
Ordered Locus Names:	YGL167C
ORF Names:	G1666

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	950 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium. Has a role in the secretory pathway.
Catalytic activity	ATP + H₂O + Ca²⁺(Cis) = ADP + phosphate + Ca²⁺(Trans).
Subcellular location	Golgi apparatus membrane; Multi-pass membrane protein.
Miscellaneous	Present with 6900 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the cation transport ATPase (P-type) family.

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Ontologies

Keywords
Biological process	Calcium transport Ion transport Transport
Cellular component	Golgi apparatus Membrane
Domain	Transmembrane
Ligand	ATP-binding Calcium Magnesium Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro calcium ion transport Inferred from direct assay. Source: SGD exocytosis Inferred from genetic interaction. Source: SGD manganese ion transport Inferred from direct assay. Source: SGD protein amino acid glycosylation Inferred from mutant phenotype. Source: SGD
Cellular component	Golgi membrane Inferred from direct assay. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from direct assay. Source: SGD calcium-transporting ATPase activity Inferred from direct assay. Source: SGD magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese-transporting ATPase activity Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
ERG11	P10614	1	EBI-3091,EBI-5127
ERG25	P53045	1	EBI-3091,EBI-6506
ERP1	Q05359	1	EBI-3091,EBI-6581
ERV25	P54837	1	EBI-3091,EBI-6642
PIS1	P06197	1	EBI-3091,EBI-13458
RBD2	Q12270	1	EBI-3091,EBI-31471
SAC1	P32368	1	EBI-3091,EBI-16210
SPF1	P39986	1	EBI-3091,EBI-3128
SWP1	Q02795	1	EBI-3091,EBI-12666
YOP1	Q12402	1	EBI-3091,EBI-37092

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 950

950

Calcium-transporting ATPase 1

PRO_0000046231

Regions

Topological domain

1 – 92

Cytoplasmic Potential

Transmembrane

93 – 111

Potential

Topological domain

112 – 116

Lumenal Potential

Transmembrane

117 – 133

Potential

Topological domain

134 – 288

155

Cytoplasmic Potential

Transmembrane

289 – 309

Potential

Topological domain

310 – 323

Lumenal Potential

Transmembrane

324 – 344

Potential

Topological domain

345 – 814

470

Cytoplasmic Potential

Transmembrane

815 – 835

Potential

Topological domain

836 – 844

Lumenal Potential

Transmembrane

845 – 862

Potential

Topological domain

863 – 884

Cytoplasmic Potential

Transmembrane

885 – 905

Potential

Topological domain

906 – 909

Lumenal Potential

Transmembrane

910 – 930

Potential

Topological domain

931 – 950

Cytoplasmic Potential

Sites

Active site

371

4-aspartylphosphate intermediate By similarity

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Sequences

Sequence

Length

Mass (Da)

Tools

P13586-1 [UniParc].

Last modified January 1, 1990. Version 1.
Checksum: 0A88271FBD6870B8
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FASTA

950

104,571

        10         20         30         40         50         60 
MSDNPFNASL LDEDSNRERE ILDATAEALS KPSPSLEYCT LSVDEALEKL DTDKNGGLRS 

        70         80         90        100        110        120 
SNEANNRRSL YGPNEITVED DESLFKKFLS NFIEDRMILL LIGSAVVSLF MGNIDDAVSI 

       130        140        150        160        170        180 
TLAIFIVVTV GFVQEYRSEK SLEALNKLVP AECHLMRCGQ ESHVLASTLV PGDLVHFRIG 

       190        200        210        220        230        240 
DRIPADIRII EAIDLSIDES NLTGENEPVH KTSQTIEKSS FNDQPNSIVP ISERSCIAYM 

       250        260        270        280        290        300 
GTLVKEGHGK GIVVGTGTNT SFGAVFEMMN NIEKPKTPLQ LTMDKLGKDL SLVSFIVIGM 

       310        320        330        340        350        360 
ICLVGIIQGR SWLEMFQISV SLAVAAIPEG LPIIVTVTLA LGVLRMAKRK AIVRRLPSVE 

       370        380        390        400        410        420 
TLGSVNVICS DKTGTLTSNH MTVSKLWCLD SMSNKLNVLS LDKNKKTKNS NGNLKNYLTE 

       430        440        450        460        470        480 
DVRETLTIGN LCNNASFSQE HAIFLGNPTD VALLEQLANF EMPDIRNTVQ KVQELPFNSK 

       490        500        510        520        530        540 
RKLMATKILN PVDNKCTVYV KGAFERILEY STSYLKSKGK KTEKLTEAQK ATINECANSM 

       550        560        570        580        590        600 
ASEGLRVFGF AKLTLSDSST PLTEDLIKDL TFTGLIGMND PPRPNVKFAI EQLLQGGVHI 

       610        620        630        640        650        660 
IMITGDSENT AVNIAKQIGI PVIDPKLSVL SGDKLDEMSD DQLANVIDHV NIFARATPEH 

       670        680        690        700        710        720 
KLNIVRALRK RGDVVAMTGD GVNDAPALKL SDIGVSMGRI GTDVAKEASD MVLTDDDFST 

       730        740        750        760        770        780 
ILTAIEEGKG IFNNIQNFLT FQLSTSVAAL SLVALSTAFK LPNPLNAMQI LWINILMDGP 

       790        800        810        820        830        840 
PAQSLGVEPV DHEVMKKPPR KRTDKILTHD VMKRLLTTAA CIIVGTVYIF VKEMAEDGKV 

       850        860        870        880        890        900 
TARDTTMTFT CFVFFDMFNA LACRHNTKSI FEIGFFTNKM FNYAVGLSLL GQMCAIYIPF 

       910        920        930        940        950 
FQSIFKTEKL GISDILLLLL ISSSVFIVDE LRKLWTRKKN EEDSTYFSNV

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The yeast secretory pathway is perturbed by mutations in PMR1, a member of a Ca2+ ATPase family." Rudolph H.K., Antebi A., Fink G.R., Buckley C.M., Dorman T.E., Levitre J., Davidow L.S., Mao J.-I., Moir D.T. Cell 58:133-145(1989) [PubMed: 2526682] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A putative helicase, the SUA5, PMR1, tRNALys1 genes and four open reading frames have been detected in the DNA sequence of an 8.8 kb fragment of the left arm of chromosome VII of Saccharomyces cerevisiae." Klima R., Coglievina M., Zaccaria P., Bertani I., Bruschi C.V. Yeast 12:1033-1040(1996) [PubMed: 8896267] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"Isolation and characterization of SUA5, a novel gene required for normal growth in Saccharomyces cerevisiae." Na J.G., Pinto I., Hampsey M. Genetics 131:791-801(1992) [PubMed: 1325384] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 863-950.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

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Cross-references

Sequence databases
EMBL GenBank DDBJ	M25488 Genomic DNA. Translation: AAA34884.1. X85757 Genomic DNA. Translation: CAA59762.1. Z72690 Genomic DNA. Translation: CAA96880.1. X64319 Genomic DNA. Translation: CAA45599.1.
PIR	PWBYR1. A30990.
RefSeq	NP_011348.1.
3D structure databases
SMR	P13586. Positions 37-939.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-7899N.
IntAct	P13586. 34 interactions.
STRING	P13586.
Protein family/group databases
TCDB	3.A.3.2.3. P-type ATPase (P-ATPase) superfamily.
Proteomic databases
PeptideAtlas	P13586.
Genome annotation databases
Ensembl	YGL167C; YGL167C; YGL167C; Saccharomyces cerevisiae. [Genome view]
GeneID	852709.
KEGG	sce:YGL167C.
NMPDR	fig\|4932.3.peg.2452.
Organism-specific databases
CYGD	YGL167c.
SGD	S000003135. PMR1.
Phylogenomic databases
eggNOG	fuNOG04517.
HOGENOM	HBG456486.
OMA	LMPPECH.
OrthoDB	EOG9PK3RD.
PhylomeDB	P13586.
Enzyme and pathway databases
BRENDA	3.6.3.8. 250.
Gene expression databases
ArrayExpress	P13586.
Genevestigator	P13586.
GermOnline	YGL167C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR008250. ATPase_P-typ_ATPase-assoc-dom. IPR006413. ATPase_P-typ_Ca-transp_PMR1. IPR006068. ATPase_P-typ_cation-transptr_C. IPR004014. ATPase_P-typ_cation-transptr_N. IPR000695. ATPase_P-typ_H-transp. IPR001757. ATPase_P-typ_ion-transptr. IPR018303. ATPase_P-typ_P_site. IPR005834. Dehalogen-like_hydro. [Graphical view]
PANTHER	PTHR11939. ATPase_P. 1 hit.
Pfam	PF00689. Cation_ATPase_C. 1 hit. PF00690. Cation_ATPase_N. 1 hit. PF00122. E1-E2_ATPase. 1 hit. PF00702. Hydrolase. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE. PR00120. HATPASE.
SMART	SM00831. Cation_ATPase_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01522. ATPase-IIA2_Ca. 1 hit. TIGR01494. ATPase_P-type. 3 hits.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	972070.

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Entry information

Entry name

ATC1_YEAST

Accession

Primary (citable) accession number: P13586

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	February 9, 2010
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents