Reviewed, UniProtKB/Swiss-Prot P13584 (CP4B1_HUMAN)

Names and origin

Protein names

Recommended name:
    Cytochrome P450 4B1
    EC=1.14.14.1
Alternative name(s):
    CYPIVB1
    P450-HP

Gene names

Name:

CYP4B1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	511 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Induction	P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Coding sequence diversity	Polymorphism
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro oxygen binding Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 511	511	Cytochrome P450 4B1		PRO_0000051819
Sites
	Metal binding	453	1	Iron (heme axial ligand)
	Binding site	315	1	Heme (covalent; via 1 link) By similarity
Natural variations
	Natural variant	111	1	A → V: dbSNP rs45559437.		VAR_055377
	Natural variant	173	1	R → W in allele CYP4B1*3 and allele CYP4B1*6. dbSNP rs4646487. Ref.3		VAR_018357
	Natural variant	264	1	R → W: dbSNP rs45446505.		VAR_048453
	Natural variant	274	1	R → Q: dbSNP rs45578838.		VAR_055378
	Natural variant	322	1	S → G in allele CYP4B1*4. dbSNP rs45467195.		VAR_018358
	Natural variant	329	1	Y → S: dbSNP rs12094024.		VAR_048454
	Natural variant	331	1	M → I in allele CYP4B1*2, allele CYP4B1*7 and allele CYP4B1*5. dbSNP rs2297810. Ref.3		VAR_018359
	Natural variant	340	1	R → C in allele CYP4B1*2 and allele CYP4B1*7. dbSNP rs4646491. Ref.3		VAR_018360
	Natural variant	345	1	V → I in allele CYP4B1*6.		VAR_018361
	Natural variant	354	1	F → C: dbSNP rs17102592.		VAR_048455
	Natural variant	375	1	R → C in allele CYP4B1*2. dbSNP rs2297809. Ref.3		VAR_018362
	Natural variant	482	1	R → Q: dbSNP rs45622937.		VAR_048456
Experimental info
	Sequence conflict	37	1	Q → R in AAA35712. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P13584-1 [UniParc]. Last modified June 7, 2004. Version 2. Checksum: 91FED40949507EB8	FASTA	511	58,991
	10 20 30 40 50 60 MVPSFLSLSF SSLGLWASGL ILVLGFLKLI HLLLRRQTLA KAMDKFPGPP THWLFGHALE 70 80 90 100 110 120 IQETGSLDKV VSWAHQFPYA HPLWFGQFIG FLNIYEPDYA KAVYSRGDPK APDVYDFFLQ 130 140 150 160 170 180 WIGRGLLVLE GPKWLQHRKL LTPGFHYDVL KPYVAVFTES TRIMLDKWEE KAREGKSFDI 190 200 210 220 230 240 FCDVGHMALN TLMKCTFGRG DTGLGHRDSS YYLAVSDLTL LMQQRLVSFQ YHNDFIYWLT 250 260 270 280 290 300 PHGRRFLRAC QVAHDHTDQV IRERKAALQD EKVRKKIQNR RHLDFLDILL GARDEDDIKL 310 320 330 340 350 360 SDADLRAEVD TFMFEGHDTT TSGISWFLYC MALYPEHQHR CREEVREILG DQDFFQWDDL 370 380 390 400 410 420 GKMTYLTMCI KESFRLYPPV PQVYRQLSKP VTFVDGRSLP AGSLISMHIY ALHRNSAVWP 430 440 450 460 470 480 DPEVFDSLRF STENASKRHP FAFMPFSAGP RNCIGQQFAM SEMKVVTAMC LLRFEFSLDP 490 500 510 SRLPIKMPQL VLRSKNGFHL HLKPLGPGSG K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a new P450 expressed in human lung: complete cDNA sequence, cDNA-directed expression, and chromosome mapping." Nhamburo P.T., Gonzalez F.J., McBride O.W., Gelboin H.V., Kimura S. Biochemistry 28:8060-8066(1989) [PubMed: 2574990] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lung.
[2]	"cDNA cloning of cytochrome P-450 related to P-450p-2 from the cDNA library of human placenta. Gene structure and expression." Yokotani N., Sogawa K., Matsubara S., Gotoh O., Kusunose E., Kusunose M., Fujii-Kuriyama Y. Eur. J. Biochem. 187:23-29(1990) [PubMed: 2298205] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Placenta.
[3]	"Genetic polymorphism of the human cytochrome P450 CYP4B1: evidence for a non-functional allelic variant." Lo-Guidice J.-M., Allorge D., Cauffiez C., Chevalier D., Lafitte J.-J., Lhermitte M., Broly F. Pharmacogenetics 12:367-374(2002) [PubMed: 12142726] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS TRP-173; GLY-322; ILE-331; CYS-340 AND CYS-375.
[4]	NIEHS SNPs program Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS VAL-111; TRP-173; TRP-264; GLN-274; GLY-322; SER-329; ILE-331; CYS-340; CYS-354; CYS-375 AND GLN-482.
[5]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
+	Additional computationally mapped references.

Web resources

Cytochrome P450 Allele Nomenclature Committee

CYP4B1 alleles

NIEHS-SNPs

Cross-references

Sequence databases
EMBLGenBankDDBJ	J02871 mRNA. Translation: AAA35712.1. X16699 mRNA. Translation: CAA34672.1. AF491285 mRNA. Translation: AAM09532.1. AY064485 mRNA. Translation: AAL57720.1. AY064486 mRNA. Translation: AAL57721.1. DQ518907 Genomic DNA. Translation: ABF47106.1. AL593856, AL356793 Genomic DNA. Translation: CAI16981.1. AL356793, AL593856 Genomic DNA. Translation: CAI22559.1.
IPI	IPI00465029.
PIR	O4HUB1. S07765.
RefSeq	NP_000770.2.
UniGene	Hs.436317
3D structure databases
HSSP	HSSP built from PDB template 1JME based on UniProtKB P14779.
ModBase	Search...
Protein-protein interaction databases
IntAct	P13584. 1 interaction.
Proteomic databases
PRIDE	P13584.
Genome annotation databases
Ensembl	ENSG00000142973. Homo sapiens. [Contig view]
GeneID	1580.
KEGG	hsa:1580.
Organism-specific databases
GeneCards	GC01P047037.
H-InvDB	HIX0000556.
HGNC	HGNC:2644. CYP4B1.
HPA	HPA004331.
MIM	124075. gene.
PharmGKB	PA27119.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	P13584.
Enzyme and pathway databases
BRENDA	1.14.14.1. 247.
Reactome	REACT_13433. Biological oxidations.
Gene expression databases
ArrayExpress	P13584.
Bgee	P13584.
CleanEx	HS_CYP4B1.
GermOnline	ENSG00000142973. Homo sapiens.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	6489.
SOURCE	Search...

Entry information

Entry name

CP4B1_HUMAN

Accession

Primary (citable) accession number: P13584
Secondary accession number(s): Q1HBI2 , Q8TD85, Q8WWU9, Q8WWV0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	June 7, 2004
Last modified:	June 16, 2009
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)