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Protein

Cytochrome P450 4B1

Gene

CYP4B1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei315 – 3151Heme (covalent; via 1 link)By similarity
Metal bindingi453 – 4531Iron (heme axial ligand)By similarity

GO - Molecular functioni

  • aromatase activity Source: UniProtKB-EC
  • drug binding Source: Ensembl
  • fluorene oxygenase activity Source: Ensembl
  • heme binding Source: InterPro
  • iron ion binding Source: InterPro
  • oxygen binding Source: ProtInc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_13425. Miscellaneous substrates.
REACT_13645. Eicosanoids.
REACT_13814. Fatty acids.
REACT_150420. Synthesis of Leukotrienes (LT) and Eoxins (EX).

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 4B1 (EC:1.14.14.1)
Alternative name(s):
CYPIVB1
Cytochrome P450-HP
Gene namesi
Name:CYP4B1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:2644. CYP4B1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA27119.

Chemistry

DrugBankiDB00683. Midazolam.
DB01174. Phenobarbital.
DB00152. Thiamine.

Polymorphism and mutation databases

BioMutaiCYP4B1.
DMDMi48429213.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 511510Cytochrome P450 4B1PRO_0000051819Add
BLAST

Proteomic databases

PaxDbiP13584.
PRIDEiP13584.

PTM databases

PhosphoSiteiP13584.

Expressioni

Tissue specificityi

Detected in the liver and lung (at protein level).1 Publication

Inductioni

P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.

Gene expression databases

BgeeiP13584.
CleanExiHS_CYP4B1.
ExpressionAtlasiP13584. baseline and differential.
GenevisibleiP13584. HS.

Organism-specific databases

HPAiHPA004331.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000360991.

Structurei

3D structure databases

ProteinModelPortaliP13584.
SMRiP13584. Positions 46-506.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118816.
HOGENOMiHOG000233833.
HOVERGENiHBG000182.
InParanoidiP13584.
KOiK07426.
OMAiKIQNQRH.
PhylomeDBiP13584.
TreeFamiTF105088.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P13584-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVPSFLSLSF SSLGLWASGL ILVLGFLKLI HLLLRRQTLA KAMDKFPGPP
60 70 80 90 100
THWLFGHALE IQETGSLDKV VSWAHQFPYA HPLWFGQFIG FLNIYEPDYA
110 120 130 140 150
KAVYSRGDPK APDVYDFFLQ WIGRGLLVLE GPKWLQHRKL LTPGFHYDVL
160 170 180 190 200
KPYVAVFTES TRIMLDKWEE KAREGKSFDI FCDVGHMALN TLMKCTFGRG
210 220 230 240 250
DTGLGHRDSS YYLAVSDLTL LMQQRLVSFQ YHNDFIYWLT PHGRRFLRAC
260 270 280 290 300
QVAHDHTDQV IRERKAALQD EKVRKKIQNR RHLDFLDILL GARDEDDIKL
310 320 330 340 350
SDADLRAEVD TFMFEGHDTT TSGISWFLYC MALYPEHQHR CREEVREILG
360 370 380 390 400
DQDFFQWDDL GKMTYLTMCI KESFRLYPPV PQVYRQLSKP VTFVDGRSLP
410 420 430 440 450
AGSLISMHIY ALHRNSAVWP DPEVFDSLRF STENASKRHP FAFMPFSAGP
460 470 480 490 500
RNCIGQQFAM SEMKVVTAMC LLRFEFSLDP SRLPIKMPQL VLRSKNGFHL
510
HLKPLGPGSG K
Length:511
Mass (Da):58,991
Last modified:June 7, 2004 - v2
Checksum:i91FED40949507EB8
GO
Isoform 2 (identifier: P13584-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     206-206: H → HS

Show »
Length:512
Mass (Da):59,078
Checksum:i1C5FB3436D612DD7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti37 – 371Q → R in AAA35712 (PubMed:2574990).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti111 – 1111A → V.1 Publication
Corresponds to variant rs45559437 [ dbSNP | Ensembl ].
VAR_055377
Natural varianti173 – 1731R → W in allele CYP4B1*3 and allele CYP4B1*6. 2 Publications
Corresponds to variant rs4646487 [ dbSNP | Ensembl ].
VAR_018357
Natural varianti264 – 2641R → W.1 Publication
Corresponds to variant rs45446505 [ dbSNP | Ensembl ].
VAR_048453
Natural varianti274 – 2741R → Q.1 Publication
Corresponds to variant rs45578838 [ dbSNP | Ensembl ].
VAR_055378
Natural varianti322 – 3221S → G in allele CYP4B1*4. 2 Publications
Corresponds to variant rs45467195 [ dbSNP | Ensembl ].
VAR_018358
Natural varianti329 – 3291Y → S.1 Publication
Corresponds to variant rs12094024 [ dbSNP | Ensembl ].
VAR_048454
Natural varianti331 – 3311M → I in allele CYP4B1*2, allele CYP4B1*7 and allele CYP4B1*5. 2 Publications
Corresponds to variant rs2297810 [ dbSNP | Ensembl ].
VAR_018359
Natural varianti340 – 3401R → C in allele CYP4B1*2 and allele CYP4B1*7. 2 Publications
Corresponds to variant rs4646491 [ dbSNP | Ensembl ].
VAR_018360
Natural varianti345 – 3451V → I in allele CYP4B1*6.
VAR_018361
Natural varianti354 – 3541F → C.1 Publication
Corresponds to variant rs17102592 [ dbSNP | Ensembl ].
VAR_048455
Natural varianti375 – 3751R → C in allele CYP4B1*2. 2 Publications
Corresponds to variant rs2297809 [ dbSNP | Ensembl ].
VAR_018362
Natural varianti482 – 4821R → Q.1 Publication
Corresponds to variant rs45622937 [ dbSNP | Ensembl ].
VAR_048456

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei206 – 2061H → HS in isoform 2. 2 PublicationsVSP_038419

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02871 mRNA. Translation: AAA35712.1.
X16699 mRNA. Translation: CAA34672.1.
AF491285 mRNA. Translation: AAM09532.1.
AY064485 mRNA. Translation: AAL57720.1.
AY064486 mRNA. Translation: AAL57721.1.
AY151048 mRNA. Translation: AAN72311.1.
DQ518907 Genomic DNA. Translation: ABF47106.1.
AL593856, AL356793 Genomic DNA. Translation: CAI16981.1.
AL593856, AL356793 Genomic DNA. Translation: CAI16982.1.
AL356793, AL593856 Genomic DNA. Translation: CAI22557.1.
AL356793, AL593856 Genomic DNA. Translation: CAI22559.1.
BC017758 mRNA. Translation: AAH17758.1.
CCDSiCCDS41328.1. [P13584-2]
CCDS542.1. [P13584-1]
PIRiS07765. O4HUB1.
RefSeqiNP_000770.2. NM_000779.3. [P13584-1]
NP_001093242.1. NM_001099772.1. [P13584-2]
UniGeneiHs.436317.

Genome annotation databases

EnsembliENST00000271153; ENSP00000271153; ENSG00000142973.
ENST00000371923; ENSP00000360991; ENSG00000142973. [P13584-2]
GeneIDi1580.
KEGGihsa:1580.
UCSCiuc001cqm.4. human. [P13584-1]
uc001cqn.4. human. [P13584-2]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

Cytochrome P450 Allele Nomenclature Committee

CYP4B1 alleles

NIEHS-SNPs
Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02871 mRNA. Translation: AAA35712.1.
X16699 mRNA. Translation: CAA34672.1.
AF491285 mRNA. Translation: AAM09532.1.
AY064485 mRNA. Translation: AAL57720.1.
AY064486 mRNA. Translation: AAL57721.1.
AY151048 mRNA. Translation: AAN72311.1.
DQ518907 Genomic DNA. Translation: ABF47106.1.
AL593856, AL356793 Genomic DNA. Translation: CAI16981.1.
AL593856, AL356793 Genomic DNA. Translation: CAI16982.1.
AL356793, AL593856 Genomic DNA. Translation: CAI22557.1.
AL356793, AL593856 Genomic DNA. Translation: CAI22559.1.
BC017758 mRNA. Translation: AAH17758.1.
CCDSiCCDS41328.1. [P13584-2]
CCDS542.1. [P13584-1]
PIRiS07765. O4HUB1.
RefSeqiNP_000770.2. NM_000779.3. [P13584-1]
NP_001093242.1. NM_001099772.1. [P13584-2]
UniGeneiHs.436317.

3D structure databases

ProteinModelPortaliP13584.
SMRiP13584. Positions 46-506.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000360991.

Chemistry

DrugBankiDB00683. Midazolam.
DB01174. Phenobarbital.
DB00152. Thiamine.

PTM databases

PhosphoSiteiP13584.

Polymorphism and mutation databases

BioMutaiCYP4B1.
DMDMi48429213.

Proteomic databases

PaxDbiP13584.
PRIDEiP13584.

Protocols and materials databases

DNASUi1580.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000271153; ENSP00000271153; ENSG00000142973.
ENST00000371923; ENSP00000360991; ENSG00000142973. [P13584-2]
GeneIDi1580.
KEGGihsa:1580.
UCSCiuc001cqm.4. human. [P13584-1]
uc001cqn.4. human. [P13584-2]

Organism-specific databases

CTDi1580.
GeneCardsiGC01P047227.
HGNCiHGNC:2644. CYP4B1.
HPAiHPA004331.
MIMi124075. gene.
neXtProtiNX_P13584.
PharmGKBiPA27119.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118816.
HOGENOMiHOG000233833.
HOVERGENiHBG000182.
InParanoidiP13584.
KOiK07426.
OMAiKIQNQRH.
PhylomeDBiP13584.
TreeFamiTF105088.

Enzyme and pathway databases

ReactomeiREACT_13425. Miscellaneous substrates.
REACT_13645. Eicosanoids.
REACT_13814. Fatty acids.
REACT_150420. Synthesis of Leukotrienes (LT) and Eoxins (EX).

Miscellaneous databases

ChiTaRSiCYP4B1. human.
GeneWikiiCYP4B1.
GenomeRNAii1580.
NextBioi6489.
PROiP13584.
SOURCEiSearch...

Gene expression databases

BgeeiP13584.
CleanExiHS_CYP4B1.
ExpressionAtlasiP13584. baseline and differential.
GenevisibleiP13584. HS.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a new P450 expressed in human lung: complete cDNA sequence, cDNA-directed expression, and chromosome mapping."
    Nhamburo P.T., Gonzalez F.J., McBride O.W., Gelboin H.V., Kimura S.
    Biochemistry 28:8060-8066(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Lung.
  2. "cDNA cloning of cytochrome P-450 related to P-450p-2 from the cDNA library of human placenta. Gene structure and expression."
    Yokotani N., Sogawa K., Matsubara S., Gotoh O., Kusunose E., Kusunose M., Fujii-Kuriyama Y.
    Eur. J. Biochem. 187:23-29(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Placenta.
  3. "Genetic polymorphism of the human cytochrome P450 CYP4B1: evidence for a non-functional allelic variant."
    Lo-Guidice J.-M., Allorge D., Cauffiez C., Chevalier D., Lafitte J.-J., Lhermitte M., Broly F.
    Pharmacogenetics 12:367-374(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANTS TRP-173; GLY-322; ILE-331; CYS-340 AND CYS-375.
  4. "Characterization of pulmonary CYP4B2, specific catalyst of methyl oxidation of 3-methylindole."
    Carr B.A., Ramakanth S., Dannan G.A., Yost G.S.
    Mol. Pharmacol. 63:1137-1147(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY.
    Tissue: Lung.
  5. NIEHS SNPs program
    Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS VAL-111; TRP-173; TRP-264; GLN-274; GLY-322; SER-329; ILE-331; CYS-340; CYS-354; CYS-375 AND GLN-482.
  6. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Lung.

Entry informationi

Entry nameiCP4B1_HUMAN
AccessioniPrimary (citable) accession number: P13584
Secondary accession number(s): Q1HBI2
, Q8TD85, Q8WWF2, Q8WWU9, Q8WWV0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: June 7, 2004
Last modified: July 22, 2015
This is version 148 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.