P13584 (CP4B1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 4B1
EC=1.14.14.1

Alternative name(s):
CYPIVB1
Cytochrome P450-HP

Gene names

Name:

CYP4B1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	511 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Tissue specificity	Detected in the liver and lung (at protein level). Ref.4
Induction	P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	biphenyl metabolic process Inferred from electronic annotation. Source: Compara exogenous drug catabolic process Inferred from electronic annotation. Source: Compara fluorene metabolic process Inferred from electronic annotation. Source: Compara small molecule metabolic process Traceable author statement. Source: Reactome xenobiotic metabolic process Traceable author statement. Source: Reactome
Cellular_component	endoplasmic reticulum membrane Traceable author statement. Source: Reactome
Molecular_function	aromatase activity Inferred from electronic annotation. Source: EC drug binding Inferred from electronic annotation. Source: Compara electron carrier activity Inferred from electronic annotation. Source: InterPro fluorene oxygenase activity Inferred from electronic annotation. Source: Compara heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro oxygen binding Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P13584-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P13584-2)

The sequence of this isoform differs from the canonical sequence as follows:
     206-206: H → HS

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 511	510	Cytochrome P450 4B1		PRO_0000051819
Sites
	Metal binding	453	1	Iron (heme axial ligand)
	Binding site	315	1	Heme (covalent; via 1 link) By similarity
Natural variations
	Alternative sequence	206	1	H → HS in isoform 2.		VSP_038419
	Natural variant	111	1	A → V. Ref.5 Corresponds to variant rs45559437 [ dbSNP | Ensembl ].		VAR_055377
	Natural variant	173	1	R → W in allele CYP4B1*3 and allele CYP4B1*6. Ref.3 Ref.5 Corresponds to variant rs4646487 [ dbSNP | Ensembl ].		VAR_018357
	Natural variant	264	1	R → W. Ref.5 Corresponds to variant rs45446505 [ dbSNP | Ensembl ].		VAR_048453
	Natural variant	274	1	R → Q. Ref.5 Corresponds to variant rs45578838 [ dbSNP | Ensembl ].		VAR_055378
	Natural variant	322	1	S → G in allele CYP4B1*4. Ref.3 Ref.5 Corresponds to variant rs45467195 [ dbSNP | Ensembl ].		VAR_018358
	Natural variant	329	1	Y → S. Ref.5 Corresponds to variant rs12094024 [ dbSNP | Ensembl ].		VAR_048454
	Natural variant	331	1	M → I in allele CYP4B1*2, allele CYP4B1*7 and allele CYP4B1*5. Ref.3 Ref.5 Corresponds to variant rs2297810 [ dbSNP | Ensembl ].		VAR_018359
	Natural variant	340	1	R → C in allele CYP4B1*2 and allele CYP4B1*7. Ref.3 Ref.5 Corresponds to variant rs4646491 [ dbSNP | Ensembl ].		VAR_018360
	Natural variant	345	1	V → I in allele CYP4B1*6.		VAR_018361
	Natural variant	354	1	F → C. Ref.5 Corresponds to variant rs17102592 [ dbSNP | Ensembl ].		VAR_048455
	Natural variant	375	1	R → C in allele CYP4B1*2. Ref.3 Ref.5 Corresponds to variant rs2297809 [ dbSNP | Ensembl ].		VAR_018362
	Natural variant	482	1	R → Q. Ref.5 Corresponds to variant rs45622937 [ dbSNP | Ensembl ].		VAR_048456
Experimental info
	Sequence conflict	37	1	Q → R in AAA35712. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 7, 2004. Version 2. Checksum: 91FED40949507EB8	FASTA	511	58,991
	10 20 30 40 50 60 MVPSFLSLSF SSLGLWASGL ILVLGFLKLI HLLLRRQTLA KAMDKFPGPP THWLFGHALE 70 80 90 100 110 120 IQETGSLDKV VSWAHQFPYA HPLWFGQFIG FLNIYEPDYA KAVYSRGDPK APDVYDFFLQ 130 140 150 160 170 180 WIGRGLLVLE GPKWLQHRKL LTPGFHYDVL KPYVAVFTES TRIMLDKWEE KAREGKSFDI 190 200 210 220 230 240 FCDVGHMALN TLMKCTFGRG DTGLGHRDSS YYLAVSDLTL LMQQRLVSFQ YHNDFIYWLT 250 260 270 280 290 300 PHGRRFLRAC QVAHDHTDQV IRERKAALQD EKVRKKIQNR RHLDFLDILL GARDEDDIKL 310 320 330 340 350 360 SDADLRAEVD TFMFEGHDTT TSGISWFLYC MALYPEHQHR CREEVREILG DQDFFQWDDL 370 380 390 400 410 420 GKMTYLTMCI KESFRLYPPV PQVYRQLSKP VTFVDGRSLP AGSLISMHIY ALHRNSAVWP 430 440 450 460 470 480 DPEVFDSLRF STENASKRHP FAFMPFSAGP RNCIGQQFAM SEMKVVTAMC LLRFEFSLDP 490 500 510 SRLPIKMPQL VLRSKNGFHL HLKPLGPGSG K
	Isoform 2 [UniParc]. Checksum: 1C5FB3436D612DD7	FASTA	512	59,078
	10 20 30 40 50 60 MVPSFLSLSF SSLGLWASGL ILVLGFLKLI HLLLRRQTLA KAMDKFPGPP THWLFGHALE 70 80 90 100 110 120 IQETGSLDKV VSWAHQFPYA HPLWFGQFIG FLNIYEPDYA KAVYSRGDPK APDVYDFFLQ 130 140 150 160 170 180 WIGRGLLVLE GPKWLQHRKL LTPGFHYDVL KPYVAVFTES TRIMLDKWEE KAREGKSFDI 190 200 210 220 230 240 FCDVGHMALN TLMKCTFGRG DTGLGHSRDS SYYLAVSDLT LLMQQRLVSF QYHNDFIYWL 250 260 270 280 290 300 TPHGRRFLRA CQVAHDHTDQ VIRERKAALQ DEKVRKKIQN RRHLDFLDIL LGARDEDDIK 310 320 330 340 350 360 LSDADLRAEV DTFMFEGHDT TTSGISWFLY CMALYPEHQH RCREEVREIL GDQDFFQWDD 370 380 390 400 410 420 LGKMTYLTMC IKESFRLYPP VPQVYRQLSK PVTFVDGRSL PAGSLISMHI YALHRNSAVW 430 440 450 460 470 480 PDPEVFDSLR FSTENASKRH PFAFMPFSAG PRNCIGQQFA MSEMKVVTAM CLLRFEFSLD 490 500 510 PSRLPIKMPQ LVLRSKNGFH LHLKPLGPGS GK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a new P450 expressed in human lung: complete cDNA sequence, cDNA-directed expression, and chromosome mapping." Nhamburo P.T., Gonzalez F.J., McBride O.W., Gelboin H.V., Kimura S. Biochemistry 28:8060-8066(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Lung.
[2]	"cDNA cloning of cytochrome P-450 related to P-450p-2 from the cDNA library of human placenta. Gene structure and expression." Yokotani N., Sogawa K., Matsubara S., Gotoh O., Kusunose E., Kusunose M., Fujii-Kuriyama Y. Eur. J. Biochem. 187:23-29(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Placenta.
[3]	"Genetic polymorphism of the human cytochrome P450 CYP4B1: evidence for a non-functional allelic variant." Lo-Guidice J.-M., Allorge D., Cauffiez C., Chevalier D., Lafitte J.-J., Lhermitte M., Broly F. Pharmacogenetics 12:367-374(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANTS TRP-173; GLY-322; ILE-331; CYS-340 AND CYS-375.
[4]	"Characterization of pulmonary CYP4B2, specific catalyst of methyl oxidation of 3-methylindole." Carr B.A., Ramakanth S., Dannan G.A., Yost G.S. Mol. Pharmacol. 63:1137-1147(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY. Tissue: Lung.
[5]	NIEHS SNPs program Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS VAL-111; TRP-173; TRP-264; GLN-274; GLY-322; SER-329; ILE-331; CYS-340; CYS-354; CYS-375 AND GLN-482.
[6]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Lung.
+	Additional computationally mapped references.

Web resources

Cytochrome P450 Allele Nomenclature Committee

CYP4B1 alleles

NIEHS-SNPs

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases
EMBL GenBank DDBJ	J02871 mRNA. Translation: AAA35712.1. X16699 mRNA. Translation: CAA34672.1. AF491285 mRNA. Translation: AAM09532.1. AY064485 mRNA. Translation: AAL57720.1. AY064486 mRNA. Translation: AAL57721.1. AY151048 mRNA. Translation: AAN72311.1. DQ518907 Genomic DNA. Translation: ABF47106.1. AL593856, AL356793 Genomic DNA. Translation: CAI16981.1. AL593856, AL356793 Genomic DNA. Translation: CAI16982.1. AL356793, AL593856 Genomic DNA. Translation: CAI22557.1. AL356793, AL593856 Genomic DNA. Translation: CAI22559.1. BC017758 mRNA. Translation: AAH17758.1.
IPI	IPI00465029. IPI00644258.
PIR	O4HUB1. S07765.
RefSeq	NP_000770.2. NM_000779.3. NP_001093242.1. NM_001099772.1.
UniGene	Hs.436317.
3D structure databases
ProteinModelPortal	P13584.
ModBase	Search...
PTM databases
PhosphoSite	P13584.
Polymorphism databases
DMDM	48429213.
Proteomic databases
PaxDb	P13584.
PRIDE	P13584.
Protocols and materials databases
DNASU	1580.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000271153; ENSP00000271153; ENSG00000142973. ENST00000371923; ENSP00000360991; ENSG00000142973.
GeneID	1580.
KEGG	hsa:1580.
UCSC	uc001cqm.4. human. uc001cqn.4. human.
Organism-specific databases
CTD	1580.
GeneCards	GC01P047264.
HGNC	HGNC:2644. CYP4B1.
HPA	HPA004331.
MIM	124075. gene.
neXtProt	NX_P13584.
PharmGKB	PA27119.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG2124.
HOGENOM	HOG000233833.
HOVERGEN	HBG000182.
KO	K07426.
OMA	WLGQFLG.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism.
Gene expression databases
ArrayExpress	P13584.
Bgee	P13584.
CleanEx	HS_CYP4B1.
Genevestigator	P13584.
GermOnline	ENSG00000142973. Homo sapiens.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CYP4B1. human.
GenomeRNAi	1580.
NextBio	6489.
SOURCE	Search...

Entry information

Entry name

CP4B1_HUMAN

Accession

Primary (citable) accession number: P13584
Secondary accession number(s): Q1HBI2 , Q8TD85, Q8WWF2, Q8WWU9, Q8WWV0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	June 7, 2004
Last modified:	May 29, 2013
This is version 130 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.