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Protein

Serine protease easter

Gene

ea

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the extracellular signaling pathway that establishes the dorsal-ventral pathway of the embryo. Three proteases; ndl, gd and snk process easter to create active easter. Active easter defines cell identities along the dorsal-ventral continuum by activating the spz ligand for the Tl receptor in the ventral region of the embryo.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei173 – 1731Charge relay systemBy similarity
Active sitei240 – 2401Charge relay systemBy similarity
Active sitei338 – 3381Charge relay systemBy similarity

GO - Molecular functioni

  • peptidase activity Source: FlyBase
  • serine-type endopeptidase activity Source: FlyBase
  • serine-type peptidase activity Source: FlyBase

GO - Biological processi

  • defense response Source: FlyBase
  • dorsal/ventral axis specification Source: FlyBase
  • maternal specification of dorsal/ventral axis, oocyte, germ-line encoded Source: FlyBase
  • proteolysis Source: FlyBase
  • Toll signaling pathway Source: FlyBase
  • zymogen activation Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.201.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine protease easter (EC:3.4.21.-)
Gene namesi
Name:ea
ORF Names:CG4920
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0000533. ea.

Subcellular locationi

  • Secreted 1 Publication

GO - Cellular componenti

  • extracellular region Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi131 – 1311G → E: Dominant allele EA 20N. 1 Publication
Mutagenesisi172 – 1721S → L: Recessive allele EA 111. 1 Publication
Mutagenesisi283 – 2831G → S: Dominant allele EA 84B. 1 Publication
Mutagenesisi324 – 3241C → Y: Recessive allele EA 818. 1 Publication
Mutagenesisi325 – 3251A → V: Dominant alleles EA 83I and EA 4102. 1 Publication
Mutagenesisi335 – 3351R → C: Dominant allele EA 125.3. 1 Publication
Mutagenesisi336 – 3361G → S: Dominant allele EA 12A. 1 Publication
Mutagenesisi338 – 3381S → A: Loss of activity. 1 Publication
Mutagenesisi339 – 3391G → R: Recessive allele EA 1. 1 Publication
Mutagenesisi360 – 3601V → M: Dominant allele EA 5.13. 1 Publication
Mutagenesisi363 – 3631G → E: Recessive allele EA 8. 1 Publication
Mutagenesisi371 – 3711G → R: Dominant allele EA 161.13. 1 Publication
Mutagenesisi373 – 3731P → S: Dominant allele EA 5022. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919CuratedAdd
BLAST
Propeptidei20 – 127108Activation peptideSequence analysisPRO_0000028133Add
BLAST
Chaini128 – 392265Serine protease easterPRO_0000028134Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi158 ↔ 174PROSITE-ProRule annotation
Disulfide bondi307 ↔ 324PROSITE-ProRule annotation
Disulfide bondi334 ↔ 367PROSITE-ProRule annotation

Post-translational modificationi

Activation peptide and active catalytic domain are associated by a disulfide bond. Processed easter is present in extremely low amounts in the early embryo as it is rapidly converted into a high molecular mass complex, which may contain a protease inhibitor. Zymogen activation is also controlled by a negative feedback loop from Dorsal.

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiP13582.
PRIDEiP13582.

Miscellaneous databases

PMAP-CutDBP13582.

Expressioni

Developmental stagei

Expressed both maternally and zygotically.1 Publication

Gene expression databases

BgeeiFBgn0000533.
GenevisibleiP13582. DM.

Interactioni

Protein-protein interaction databases

BioGridi66921. 8 interactions.
DIPiDIP-18843N.
IntActiP13582. 1 interaction.
MINTiMINT-1565820.
STRINGi7227.FBpp0082539.

Structurei

3D structure databases

ProteinModelPortaliP13582.
SMRiP13582. Positions 37-392.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini128 – 391264Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
InParanoidiP13582.
OrthoDBiEOG091G0DF7.
PhylomeDBiP13582.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR022700. CLIP.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF12032. CLIP. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00680. CLIP. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13582-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKPSIICLF LGILAKSSAG QFYFPNEAAQ VPNYGRCITP NRERALCIHL
60 70 80 90 100
EDCKYLYGLL TTTPLRDTDR LYLSRSQCGY TNGKVLICCP DRYRESSSET
110 120 130 140 150
TPPPKPNVTS NSLLPLPGQC GNILSNRIYG GMKTKIDEFP WMALIEYTKS
160 170 180 190 200
QGKKGHHCGG SLISTRYVIT ASHCVNGKAL PTDWRLSGVR LGEWDTNTNP
210 220 230 240 250
DCEVDVRGMK DCAPPHLDVP VERTIPHPDY IPASKNQVND IALLRLAQQV
260 270 280 290 300
EYTDFVRPIC LPLDVNLRSA TFDGITMDVA GWGKTEQLSA SNLKLKAAVE
310 320 330 340 350
GSRMDECQNV YSSQDILLED TQMCAGGKEG VDSCRGDSGG PLIGLDTNKV
360 370 380 390
NTYYFLAGVV SFGPTPCGLA GWPGVYTLVG KYVDWIQNTI ES
Length:392
Mass (Da):43,065
Last modified:November 7, 2003 - v3
Checksum:i1309C41E6FC176B6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti302 – 3021S → F in AAF55170 (PubMed:10731132).Curated
Sequence conflicti302 – 3021S → F in AAQ22447 (PubMed:10731132).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03154 mRNA. Translation: AAA28496.1.
AE014297 Genomic DNA. Translation: AAF55170.1.
BT009978 mRNA. Translation: AAQ22447.1.
PIRiA30100.
RefSeqiNP_524362.2. NM_079638.3.
UniGeneiDm.2421.

Genome annotation databases

GeneIDi41858.
KEGGidme:Dmel_CG4920.
UCSCiCG4920-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03154 mRNA. Translation: AAA28496.1.
AE014297 Genomic DNA. Translation: AAF55170.1.
BT009978 mRNA. Translation: AAQ22447.1.
PIRiA30100.
RefSeqiNP_524362.2. NM_079638.3.
UniGeneiDm.2421.

3D structure databases

ProteinModelPortaliP13582.
SMRiP13582. Positions 37-392.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi66921. 8 interactions.
DIPiDIP-18843N.
IntActiP13582. 1 interaction.
MINTiMINT-1565820.
STRINGi7227.FBpp0082539.

Protein family/group databases

MEROPSiS01.201.

Proteomic databases

PaxDbiP13582.
PRIDEiP13582.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi41858.
KEGGidme:Dmel_CG4920.
UCSCiCG4920-RA. d. melanogaster.

Organism-specific databases

CTDi41858.
FlyBaseiFBgn0000533. ea.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
InParanoidiP13582.
OrthoDBiEOG091G0DF7.
PhylomeDBiP13582.

Miscellaneous databases

GenomeRNAii41858.
PMAP-CutDBP13582.
PROiP13582.

Gene expression databases

BgeeiFBgn0000533.
GenevisibleiP13582. DM.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR022700. CLIP.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF12032. CLIP. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00680. CLIP. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEAST_DROME
AccessioniPrimary (citable) accession number: P13582
Secondary accession number(s): Q9VF90
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: November 7, 2003
Last modified: September 7, 2016
This is version 138 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.