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Protein

Serine protease easter

Gene

ea

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the extracellular signaling pathway that establishes the dorsal-ventral pathway of the embryo. Three proteases; ndl, gd and snk process easter to create active easter. Active easter defines cell identities along the dorsal-ventral continuum by activating the spz ligand for the Tl receptor in the ventral region of the embryo.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei173Charge relay systemBy similarity1
Active sitei240Charge relay systemBy similarity1
Active sitei338Charge relay systemBy similarity1

GO - Molecular functioni

  • peptidase activity Source: FlyBase
  • serine-type endopeptidase activity Source: FlyBase
  • serine-type peptidase activity Source: FlyBase

GO - Biological processi

  • dorsal/ventral axis specification Source: FlyBase
  • maternal specification of dorsal/ventral axis, oocyte, germ-line encoded Source: FlyBase
  • proteolysis Source: FlyBase
  • Toll signaling pathway Source: FlyBase
  • zymogen activation Source: FlyBase

Keywordsi

Molecular functionDevelopmental protein, Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.201.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine protease easter (EC:3.4.21.-)
Gene namesi
Name:ea
ORF Names:CG4920
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0000533. ea.

Subcellular locationi

  • Secreted 1 Publication

GO - Cellular componenti

  • extracellular region Source: FlyBase

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi131G → E: Dominant allele EA 20N. 1 Publication1
Mutagenesisi172S → L: Recessive allele EA 111. 1 Publication1
Mutagenesisi283G → S: Dominant allele EA 84B. 1 Publication1
Mutagenesisi324C → Y: Recessive allele EA 818. 1 Publication1
Mutagenesisi325A → V: Dominant alleles EA 83I and EA 4102. 1 Publication1
Mutagenesisi335R → C: Dominant allele EA 125.3. 1 Publication1
Mutagenesisi336G → S: Dominant allele EA 12A. 1 Publication1
Mutagenesisi338S → A: Loss of activity. 1 Publication1
Mutagenesisi339G → R: Recessive allele EA 1. 1 Publication1
Mutagenesisi360V → M: Dominant allele EA 5.13. 1 Publication1
Mutagenesisi363G → E: Recessive allele EA 8. 1 Publication1
Mutagenesisi371G → R: Dominant allele EA 161.13. 1 Publication1
Mutagenesisi373P → S: Dominant allele EA 5022. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19CuratedAdd BLAST19
PropeptideiPRO_000002813320 – 127Activation peptideSequence analysisAdd BLAST108
ChainiPRO_0000028134128 – 392Serine protease easterAdd BLAST265

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi158 ↔ 174PROSITE-ProRule annotation
Disulfide bondi307 ↔ 324PROSITE-ProRule annotation
Disulfide bondi334 ↔ 367PROSITE-ProRule annotation

Post-translational modificationi

Activation peptide and active catalytic domain are associated by a disulfide bond. Processed easter is present in extremely low amounts in the early embryo as it is rapidly converted into a high molecular mass complex, which may contain a protease inhibitor. Zymogen activation is also controlled by a negative feedback loop from Dorsal.

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiP13582.
PRIDEiP13582.

Miscellaneous databases

PMAP-CutDBiP13582.

Expressioni

Developmental stagei

Expressed both maternally and zygotically.1 Publication

Gene expression databases

BgeeiFBgn0000533.
ExpressionAtlasiP13582. differential.
GenevisibleiP13582. DM.

Interactioni

Protein-protein interaction databases

BioGridi66921. 8 interactors.
DIPiDIP-18843N.
IntActiP13582. 1 interactor.
MINTiMINT-1565820.
STRINGi7227.FBpp0082539.

Structurei

3D structure databases

ProteinModelPortaliP13582.
SMRiP13582.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini128 – 391Peptidase S1PROSITE-ProRule annotationAdd BLAST264

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
InParanoidiP13582.
OrthoDBiEOG091G0DF7.
PhylomeDBiP13582.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiView protein in InterPro
IPR022700. CLIP.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
PfamiView protein in Pfam
PF12032. CLIP. 1 hit.
PF00089. Trypsin. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiView protein in SMART
SM00680. CLIP. 1 hit.
SM00020. Tryp_SPc. 1 hit.
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiView protein in PROSITE
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13582-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKPSIICLF LGILAKSSAG QFYFPNEAAQ VPNYGRCITP NRERALCIHL
60 70 80 90 100
EDCKYLYGLL TTTPLRDTDR LYLSRSQCGY TNGKVLICCP DRYRESSSET
110 120 130 140 150
TPPPKPNVTS NSLLPLPGQC GNILSNRIYG GMKTKIDEFP WMALIEYTKS
160 170 180 190 200
QGKKGHHCGG SLISTRYVIT ASHCVNGKAL PTDWRLSGVR LGEWDTNTNP
210 220 230 240 250
DCEVDVRGMK DCAPPHLDVP VERTIPHPDY IPASKNQVND IALLRLAQQV
260 270 280 290 300
EYTDFVRPIC LPLDVNLRSA TFDGITMDVA GWGKTEQLSA SNLKLKAAVE
310 320 330 340 350
GSRMDECQNV YSSQDILLED TQMCAGGKEG VDSCRGDSGG PLIGLDTNKV
360 370 380 390
NTYYFLAGVV SFGPTPCGLA GWPGVYTLVG KYVDWIQNTI ES
Length:392
Mass (Da):43,065
Last modified:November 7, 2003 - v3
Checksum:i1309C41E6FC176B6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti302S → F in AAF55170 (PubMed:10731132).Curated1
Sequence conflicti302S → F in AAQ22447 (PubMed:10731132).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03154 mRNA. Translation: AAA28496.1.
AE014297 Genomic DNA. Translation: AAF55170.1.
BT009978 mRNA. Translation: AAQ22447.1.
PIRiA30100.
RefSeqiNP_524362.2. NM_079638.3.
UniGeneiDm.2421.

Genome annotation databases

GeneIDi41858.
KEGGidme:Dmel_CG4920.
UCSCiCG4920-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03154 mRNA. Translation: AAA28496.1.
AE014297 Genomic DNA. Translation: AAF55170.1.
BT009978 mRNA. Translation: AAQ22447.1.
PIRiA30100.
RefSeqiNP_524362.2. NM_079638.3.
UniGeneiDm.2421.

3D structure databases

ProteinModelPortaliP13582.
SMRiP13582.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi66921. 8 interactors.
DIPiDIP-18843N.
IntActiP13582. 1 interactor.
MINTiMINT-1565820.
STRINGi7227.FBpp0082539.

Protein family/group databases

MEROPSiS01.201.

Proteomic databases

PaxDbiP13582.
PRIDEiP13582.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi41858.
KEGGidme:Dmel_CG4920.
UCSCiCG4920-RA. d. melanogaster.

Organism-specific databases

CTDi41858.
FlyBaseiFBgn0000533. ea.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
InParanoidiP13582.
OrthoDBiEOG091G0DF7.
PhylomeDBiP13582.

Miscellaneous databases

GenomeRNAii41858.
PMAP-CutDBiP13582.
PROiPR:P13582.

Gene expression databases

BgeeiFBgn0000533.
ExpressionAtlasiP13582. differential.
GenevisibleiP13582. DM.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiView protein in InterPro
IPR022700. CLIP.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
PfamiView protein in Pfam
PF12032. CLIP. 1 hit.
PF00089. Trypsin. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiView protein in SMART
SM00680. CLIP. 1 hit.
SM00020. Tryp_SPc. 1 hit.
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiView protein in PROSITE
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiEAST_DROME
AccessioniPrimary (citable) accession number: P13582
Secondary accession number(s): Q9VF90
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: November 7, 2003
Last modified: April 12, 2017
This is version 145 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.