P13579 (GLB4_LUMTE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Extracellular globin-4 Alternative name(s): Erythrocruorin Globin A Globin IV
Organism	Lumbricus terrestris (Common earthworm)
Taxonomic identifier	6398 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Lophotrochozoa › Annelida › Clitellata › Oligochaeta › Haplotaxida › Lumbricina › Lumbricidae › Lumbricinae › Lumbricus › Lumbricus terrestris complex

Protein attributes

Sequence length	151 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	The extracellular hemoglobin of the earthworm consists of 12 subunits that have a hexagonal bilayer structure with a molecular weight near 3.8 million. Each one-twelfth subunit is composed primarily of disulfide linked trimers (chains A, B, and C) and monomers (chain D). Ref.3
Subcellular location	Secreted.
Sequence similarities	Belongs to the globin family.

Ontologies

Keywords
Biological process	Oxygen transport Transport
Cellular component	Secreted
Ligand	Heme Iron Metal-binding
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell hemoglobin complex Inferred from electronic annotation. Source: InterPro
Molecular_function	heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro oxygen binding Inferred from electronic annotation. Source: InterPro oxygen transporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 151

151

Extracellular globin-4

PRO_0000052515

Sites

Metal binding

101

1

Iron (heme proximal ligand)

Amino acid modifications

Disulfide bond

6

Interchain (with chain III) By similarity

Disulfide bond

7 ↔ 138

By similarity

Disulfide bond

129

Interchain (with chain I) By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

151

Helix Strand Turn

Details...

Helix

9 – 22

14

Beta strand

26 – 28

3

Helix

29 – 45

17

Helix

47 – 53

7

Turn

54 – 59

6

Helix

65 – 81

17

Turn

82 – 85

4

Helix

87 – 102

16

Helix

109 – 126

18

Beta strand

127 – 129

3

Helix

132 – 146

15

Turn

147 – 149

3

Sequences

	Sequence		Length	Mass (Da)
	P13579 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: 4A2C7421F0DCBC2F	FASTA	151	17,525
	10 20 30 40 50 60 ADDEDCCSYE DRREIRHIWD DVWSSSFTDR RVAIVRAVFD DLFKHYPTSK ALFERVKIDE 70 80 90 100 110 120 PESGEFKSHL VRVANGLDLL INLLDDTLVL QSHLGHLADQ HIQRKGVTKE YFRGIGEAFA 130 140 150 RVLPQVLSCF NVDAWNRCFH RLVARIAKDL P

References

[1]	"The amino acid sequences of chains a, b, and c that form the trimer subunit of the extracellular hemoglobin from Lumbricus terrestris." Fushitani K., Matsuura M.S.A., Riggs A.F. J. Biol. Chem. 263:6502-6517(1988) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE.
[2]	"Characterization of the constituent polypeptides of the extracellular hemoglobin from Lumbricus terrestris: heterogeneity and discovery of a new linker chain L4." Fushitani K., Higashiyama K., Asao M., Hosokawa K. Biochim. Biophys. Acta 1292:273-280(1996) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-10.
[3]	"Crystal structure of the hemoglobin dodecamer from Lumbricus erythrocruorin: allosteric core of giant annelid respiratory complexes." Strand K., Knapp J.E., Bhyravbhatla B., Royer W.E. Jr. J. Mol. Biol. 344:119-134(2004) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS) IN COMPLEX WITH HEME, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

PIR

A28151.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1X9F	X-ray	2.60	A/E/I	1-151	[»]
2GTL	X-ray	3.50	A/E/I	1-151	[»]

ProteinModelPortal

P13579.

SMR

P13579. Positions 5-151.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-29122N.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

1.10.490.10. 1 hit.

InterPro

IPR000971. Globin.
IPR009050. Globin-like.
IPR012292. Globin_dom.
IPR014610. Haemoglobin_extracell.
[Graphical view]

Pfam

PF00042. Globin. 1 hit.
[Graphical view]

PIRSF

PIRSF036517. Ext_hemo. 1 hit.

SUPFAM

SSF46458. Globin_like. 1 hit.

PROSITE

PS01033. GLOBIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P13579.

Entry information

Entry name

GLB4_LUMTE

Accession

Primary (citable) accession number: P13579

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	May 1, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)