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Protein

Extracellular globin-4

Gene
N/A
Organism
Lumbricus terrestris (Common earthworm)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi101 – 1011Iron (heme proximal ligand)

GO - Molecular functioni

Complete GO annotation...

Keywords - Biological processi

Oxygen transport, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Extracellular globin-4
Alternative name(s):
Erythrocruorin
Globin A
Globin IV
OrganismiLumbricus terrestris (Common earthworm)
Taxonomic identifieri6398 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaAnnelidaClitellataOligochaetaHaplotaxidaLumbricinaLumbricidaeLumbricinaeLumbricus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 151151Extracellular globin-4PRO_0000052515Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi6 – 6Interchain (with chain III)By similarity
Disulfide bondi7 ↔ 138By similarity
Disulfide bondi129 – 129Interchain (with chain I)By similarity

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

The extracellular hemoglobin of the earthworm consists of 12 subunits that have a hexagonal bilayer structure with a molecular weight near 3.8 million. Each one-twelfth subunit is composed primarily of disulfide linked trimers (chains A, B, and C) and monomers (chain D).1 Publication

Protein-protein interaction databases

DIPiDIP-29122N.

Structurei

Secondary structure

1
151
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi9 – 2214Combined sources
Beta strandi26 – 283Combined sources
Helixi29 – 4517Combined sources
Helixi47 – 537Combined sources
Turni54 – 596Combined sources
Helixi65 – 8117Combined sources
Turni82 – 854Combined sources
Helixi87 – 10216Combined sources
Helixi109 – 12618Combined sources
Beta strandi127 – 1293Combined sources
Helixi132 – 14615Combined sources
Turni147 – 1493Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1X9FX-ray2.60A/E/I1-151[»]
2GTLX-ray3.50A/E/I1-151[»]
4V93electron microscopy8.10A2/A3/A7/AA/AE/AF/AJ/AK/AO/AP/AT/AU/AY/AZ/Ad/Ae/Ai/Aj/An/Ao/As/At/Ax/Ay5-151[»]
B1/B6/BD/BI/BN/BS/BX/Bc/Bh/Bm/Br/Bw1-151[»]
ProteinModelPortaliP13579.
SMRiP13579. Positions 5-151.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP13579.

Family & Domainsi

Sequence similaritiesi

Belongs to the globin family.PROSITE-ProRule annotation

Family and domain databases

Gene3Di1.10.490.10. 1 hit.
InterProiIPR000971. Globin.
IPR009050. Globin-like.
IPR012292. Globin/Proto.
IPR014610. Haemoglobin_extracell.
[Graphical view]
PfamiPF00042. Globin. 1 hit.
[Graphical view]
PIRSFiPIRSF036517. Ext_hemo. 1 hit.
SUPFAMiSSF46458. SSF46458. 1 hit.
PROSITEiPS01033. GLOBIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P13579-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
ADDEDCCSYE DRREIRHIWD DVWSSSFTDR RVAIVRAVFD DLFKHYPTSK
60 70 80 90 100
ALFERVKIDE PESGEFKSHL VRVANGLDLL INLLDDTLVL QSHLGHLADQ
110 120 130 140 150
HIQRKGVTKE YFRGIGEAFA RVLPQVLSCF NVDAWNRCFH RLVARIAKDL

P
Length:151
Mass (Da):17,525
Last modified:January 1, 1990 - v1
Checksum:i4A2C7421F0DCBC2F
GO

Sequence databases

PIRiA28151.

Cross-referencesi

Sequence databases

PIRiA28151.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1X9FX-ray2.60A/E/I1-151[»]
2GTLX-ray3.50A/E/I1-151[»]
4V93electron microscopy8.10A2/A3/A7/AA/AE/AF/AJ/AK/AO/AP/AT/AU/AY/AZ/Ad/Ae/Ai/Aj/An/Ao/As/At/Ax/Ay5-151[»]
B1/B6/BD/BI/BN/BS/BX/Bc/Bh/Bm/Br/Bw1-151[»]
ProteinModelPortaliP13579.
SMRiP13579. Positions 5-151.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29122N.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP13579.

Family and domain databases

Gene3Di1.10.490.10. 1 hit.
InterProiIPR000971. Globin.
IPR009050. Globin-like.
IPR012292. Globin/Proto.
IPR014610. Haemoglobin_extracell.
[Graphical view]
PfamiPF00042. Globin. 1 hit.
[Graphical view]
PIRSFiPIRSF036517. Ext_hemo. 1 hit.
SUPFAMiSSF46458. SSF46458. 1 hit.
PROSITEiPS01033. GLOBIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The amino acid sequences of chains a, b, and c that form the trimer subunit of the extracellular hemoglobin from Lumbricus terrestris."
    Fushitani K., Matsuura M.S.A., Riggs A.F.
    J. Biol. Chem. 263:6502-6517(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.
  2. "Characterization of the constituent polypeptides of the extracellular hemoglobin from Lumbricus terrestris: heterogeneity and discovery of a new linker chain L4."
    Fushitani K., Higashiyama K., Asao M., Hosokawa K.
    Biochim. Biophys. Acta 1292:273-280(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-10.
  3. "Crystal structure of the hemoglobin dodecamer from Lumbricus erythrocruorin: allosteric core of giant annelid respiratory complexes."
    Strand K., Knapp J.E., Bhyravbhatla B., Royer W.E. Jr.
    J. Mol. Biol. 344:119-134(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS) IN COMPLEX WITH HEME, SUBUNIT.

Entry informationi

Entry nameiGLB4_LUMTE
AccessioniPrimary (citable) accession number: P13579
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: October 14, 2015
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.