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P13574

- STE12_YEAST

UniProt

P13574 - STE12_YEAST

Protein

Protein STE12

Gene

STE12

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 137 (01 Oct 2014)
      Sequence version 1 (01 Jan 1990)
      Previous versions | rss
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    Functioni

    Binds to the DNA sequence mediating pheromone induction (called the pheromone response element = PRE) which is found in the upstream control region of several a-, alpha- and haploid-specific genes. Involved in mating of haploids and in pseudohyphae formation in diploids.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi57 – 167111Add
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. sequence-specific DNA binding transcription factor activity Source: SGD

    GO - Biological processi

    1. conjugation with cellular fusion Source: SGD
    2. invasive growth in response to glucose limitation Source: SGD
    3. negative regulation of transcription from RNA polymerase II promoter by pheromones Source: SGD
    4. positive regulation of transcription from RNA polymerase II promoter by pheromones Source: SGD
    5. pseudohyphal growth Source: SGD
    6. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Pheromone response, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31131-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein STE12
    Gene namesi
    Name:STE12
    Ordered Locus Names:YHR084W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VIII

    Organism-specific databases

    CYGDiYHR084w.
    SGDiS000001126. STE12.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleoplasm Source: SGD
    2. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 688688Protein STE12PRO_0000072262Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei29 – 291Phosphothreonine2 Publications
    Modified residuei214 – 2141Phosphoserine1 Publication
    Modified residuei226 – 2261Phosphoserine3 Publications
    Modified residuei289 – 2891Phosphothreonine1 Publication
    Modified residuei400 – 4001Phosphoserine2 Publications

    Post-translational modificationi

    Phosphorylated by the STE7, STE11 and STE20 kinases.3 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP13574.
    PaxDbiP13574.
    PeptideAtlasiP13574.

    Expressioni

    Gene expression databases

    GenevestigatoriP13574.

    Interactioni

    Subunit structurei

    Interacts with mating-type protein ALPHA1.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    DIG1Q030633EBI-18264,EBI-29752
    DIG2Q033733EBI-18264,EBI-34019
    HMLALPHA1P013652EBI-18264,EBI-10438
    KSS1P146816EBI-18264,EBI-9945
    SLT2Q007722EBI-18264,EBI-17372

    Protein-protein interaction databases

    BioGridi36519. 94 interactions.
    DIPiDIP-64N.
    IntActiP13574. 23 interactions.
    MINTiMINT-405050.
    STRINGi4932.YHR084W.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3W3WX-ray2.20B581-649[»]
    ProteinModelPortaliP13574.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    The DNA-binding domain seems to be involved in the suppression of pseudohyphae formation under nitrogen-rich conditions and in haploids. This region is also involved in the regulation of budding pattern of haploids.

    Sequence similaritiesi

    Belongs to the STE12 transcription factor family.Curated

    Phylogenomic databases

    eggNOGiNOG80751.
    HOGENOMiHOG000000882.
    KOiK11215.
    OMAiCKWTSAN.
    OrthoDBiEOG7J44MH.

    Family and domain databases

    InterProiIPR003120. TF_STE.
    [Graphical view]
    PfamiPF02200. STE. 1 hit.
    [Graphical view]
    ProDomiPD018365. TF_STE. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00424. STE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P13574-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKVQITNSRT EEILKVQANN ENDEVSKATP GEVEESLRLI GDLKFFLATA    50
    PVNWQENQII RRYYLNSGQG FVSCVFWNNL YYITGTDIVK CCLYRMQKFG 100
    REVVQKKKFE EGIFSDLRNL KCGIDATLEQ PKSEFLSFLF RNMCLKTQKK 150
    QKVFFWFSVA HDKLFADALE RDLKRESLNQ PSTTKPVNEP ALSFSYDSSS 200
    DKPLYDQLLQ HLDSRRPSST TKSDNSPPKL ESENFKDNEL VTVTNQPLLG 250
    VGLMDDDAPE SPSQINDFIP QKLIIEPNTL ELNGLTEETP HDLPKNTAKG 300
    RDEEDFPLDY FPVSVEYPTE ENAFDPFPPQ AFTPAAPSMP ISYDNVNERD 350
    SMPVNSLLNR YPYQLSVAPT FPVPPSSSRQ HFMTNRDFYS SNNNKEKLVS 400
    PSDPTSYMKY DEPVMDFDES RPNENCTNAK SHNSGQQTKQ HQLYSNNFQQ 450
    SYPNGMVPGY YPKMPYNPMG GDPLLDQAFY GADDFFFPPE GCDNNMLYPQ 500
    TATSWNVLPP QAMQPAPTYV GRPYTPNYRS TPGSAMFPYM QSSNSMQWNT 550
    AVSPYSSRAP STTAKNYPPS TFYSQNINQY PRRRTVGMKS SQGNVPTGNK 600
    QSVGKSAKIS KPLHIKTSAY QKQYKINLET KARPSAGDED SAHPDKNKEI 650
    SMPTPDSNTL VVQSEEGGAH SLEVDTNRRS DKNLPDAT 688
    Length:688
    Mass (Da):77,867
    Last modified:January 1, 1990 - v1
    Checksum:iEE7371F18BBC0337
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X16112 Genomic DNA. Translation: CAA34246.1.
    M24502 Genomic DNA. Translation: AAA35109.1.
    U10556 Genomic DNA. Translation: AAB68884.1.
    BK006934 Genomic DNA. Translation: DAA06779.1.
    PIRiA33540.
    RefSeqiNP_011952.1. NM_001179214.1.

    Genome annotation databases

    EnsemblFungiiYHR084W; YHR084W; YHR084W.
    GeneIDi856484.
    KEGGisce:YHR084W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X16112 Genomic DNA. Translation: CAA34246.1 .
    M24502 Genomic DNA. Translation: AAA35109.1 .
    U10556 Genomic DNA. Translation: AAB68884.1 .
    BK006934 Genomic DNA. Translation: DAA06779.1 .
    PIRi A33540.
    RefSeqi NP_011952.1. NM_001179214.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3W3W X-ray 2.20 B 581-649 [» ]
    ProteinModelPortali P13574.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36519. 94 interactions.
    DIPi DIP-64N.
    IntActi P13574. 23 interactions.
    MINTi MINT-405050.
    STRINGi 4932.YHR084W.

    Proteomic databases

    MaxQBi P13574.
    PaxDbi P13574.
    PeptideAtlasi P13574.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YHR084W ; YHR084W ; YHR084W .
    GeneIDi 856484.
    KEGGi sce:YHR084W.

    Organism-specific databases

    CYGDi YHR084w.
    SGDi S000001126. STE12.

    Phylogenomic databases

    eggNOGi NOG80751.
    HOGENOMi HOG000000882.
    KOi K11215.
    OMAi CKWTSAN.
    OrthoDBi EOG7J44MH.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31131-MONOMER.

    Miscellaneous databases

    NextBioi 982176.

    Gene expression databases

    Genevestigatori P13574.

    Family and domain databases

    InterProi IPR003120. TF_STE.
    [Graphical view ]
    Pfami PF02200. STE. 1 hit.
    [Graphical view ]
    ProDomi PD018365. TF_STE. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SMARTi SM00424. STE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The yeast STE12 protein binds to the DNA sequence mediating pheromone induction."
      Dolan J.W., Kirkman C., Fields S.
      Proc. Natl. Acad. Sci. U.S.A. 86:5703-5707(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "STE12, a protein involved in cell-type-specific transcription and signal transduction in yeast, is part of protein-DNA complexes."
      Errede B., Ammerer G.
      Genes Dev. 3:1349-1361(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Properties of the DNA-binding domain of the Saccharomyces cerevisiae STE12 protein."
      Yuan Y.-L., Fields S.
      Mol. Cell. Biol. 11:5910-5918(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: DNA-BINDING DOMAIN.
    6. "Coupling of cell identity to signal response in yeast: interaction between the alpha-1 and STE12 proteins."
      Yuan Y.-L.O., Stroke I., Fields S.
      Genes Dev. 7:1584-1597(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ALPHA1.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-29; SER-226 AND SER-400, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-29; SER-226 AND THR-289, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-214; SER-226 AND SER-400, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSTE12_YEAST
    AccessioniPrimary (citable) accession number: P13574
    Secondary accession number(s): D3DL35
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: January 1, 1990
    Last modified: October 1, 2014
    This is version 137 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1920 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VIII
      Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

    External Data

    Dasty 3