Protein STE12 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P13574 (STE12_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 124. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Protein STE12

Gene names

Name:	STE12
Ordered Locus Names:	YHR084W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	688 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to the DNA sequence mediating pheromone induction (called the pheromone response element = PRE) which is found in the upstream control region of several a-, alpha- and haploid-specific genes. Involved in mating of haploids and in pseudohyphae formation in diploids.
Subunit structure	Interacts with mating-type protein ALPHA1. Ref.6
Subcellular location	Nucleus.
Domain	The DNA-binding domain seems to be involved in the suppression of pseudohyphae formation under nitrogen-rich conditions and in haploids. This region is also involved in the regulation of budding pattern of haploids. Ref.5
Post-translational modification	Phosphorylated by the STE7, STE11 and STE20 kinases Probable.
Miscellaneous	Present with 1920 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the STE12 transcription factor family.

Ontologies

Keywords
Biological process	Pheromone response Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	conjugation with cellular fusion Traceable author statement PubMed 10704467. Source: SGD invasive growth in response to glucose limitation Inferred from mutant phenotype PubMed 8001818. Source: SGD negative regulation of transcription from RNA polymerase II promoter by pheromones Inferred from mutant phenotype PubMed 16940175. Source: SGD positive regulation of transcription from RNA polymerase II promoter by pheromones Inferred from mutant phenotype PubMed 7834739. Source: SGD pseudohyphal growth Inferred from mutant phenotype PubMed 8259520 PubMed 9055077. Source: SGD transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleoplasm Inferred from direct assay PubMed 20852627. Source: SGD
Molecular_function	sequence-specific DNA binding Inferred from direct assay PubMed 19111667. Source: SGD sequence-specific DNA binding transcription factor activity Inferred from physical interaction Ref.2. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
DIG1	Q03063	3	EBI-18264,EBI-29752
DIG2	Q03373	3	EBI-18264,EBI-34019
HMLALPHA1	P01365	2	EBI-18264,EBI-10438
KSS1	P14681	9	EBI-18264,EBI-9945
SLT2	Q00772	2	EBI-18264,EBI-17372

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 688

688

Protein STE12

PRO_0000072262

Regions

DNA binding

57 – 167

111

Ref.5

Amino acid modifications

Modified residue

Phosphothreonine Ref.9 Ref.12

Modified residue

218

Phosphoserine Ref.10

Modified residue

223

Phosphoserine Ref.12

Modified residue

226

Phosphoserine Ref.9 Ref.10 Ref.11 Ref.12

Modified residue

261

Phosphoserine Ref.12

Modified residue

289

Phosphothreonine Ref.11 Ref.12

Modified residue

400

Phosphoserine Ref.8 Ref.9 Ref.11 Ref.12

Sequences

Sequence

Length

Mass (Da)

Tools

P13574 [UniParc].

Last modified January 1, 1990. Version 1.
Checksum: EE7371F18BBC0337
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FASTA

688

77,867

        10         20         30         40         50         60 
MKVQITNSRT EEILKVQANN ENDEVSKATP GEVEESLRLI GDLKFFLATA PVNWQENQII 

        70         80         90        100        110        120 
RRYYLNSGQG FVSCVFWNNL YYITGTDIVK CCLYRMQKFG REVVQKKKFE EGIFSDLRNL 

       130        140        150        160        170        180 
KCGIDATLEQ PKSEFLSFLF RNMCLKTQKK QKVFFWFSVA HDKLFADALE RDLKRESLNQ 

       190        200        210        220        230        240 
PSTTKPVNEP ALSFSYDSSS DKPLYDQLLQ HLDSRRPSST TKSDNSPPKL ESENFKDNEL 

       250        260        270        280        290        300 
VTVTNQPLLG VGLMDDDAPE SPSQINDFIP QKLIIEPNTL ELNGLTEETP HDLPKNTAKG 

       310        320        330        340        350        360 
RDEEDFPLDY FPVSVEYPTE ENAFDPFPPQ AFTPAAPSMP ISYDNVNERD SMPVNSLLNR 

       370        380        390        400        410        420 
YPYQLSVAPT FPVPPSSSRQ HFMTNRDFYS SNNNKEKLVS PSDPTSYMKY DEPVMDFDES 

       430        440        450        460        470        480 
RPNENCTNAK SHNSGQQTKQ HQLYSNNFQQ SYPNGMVPGY YPKMPYNPMG GDPLLDQAFY 

       490        500        510        520        530        540 
GADDFFFPPE GCDNNMLYPQ TATSWNVLPP QAMQPAPTYV GRPYTPNYRS TPGSAMFPYM 

       550        560        570        580        590        600 
QSSNSMQWNT AVSPYSSRAP STTAKNYPPS TFYSQNINQY PRRRTVGMKS SQGNVPTGNK 

       610        620        630        640        650        660 
QSVGKSAKIS KPLHIKTSAY QKQYKINLET KARPSAGDED SAHPDKNKEI SMPTPDSNTL 

       670        680 
VVQSEEGGAH SLEVDTNRRS DKNLPDAT

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The yeast STE12 protein binds to the DNA sequence mediating pheromone induction." Dolan J.W., Kirkman C., Fields S. Proc. Natl. Acad. Sci. U.S.A. 86:5703-5707(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"STE12, a protein involved in cell-type-specific transcription and signal transduction in yeast, is part of protein-DNA complexes." Errede B., Ammerer G. Genes Dev. 3:1349-1361(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Properties of the DNA-binding domain of the Saccharomyces cerevisiae STE12 protein." Yuan Y.-L., Fields S. Mol. Cell. Biol. 11:5910-5918(1991) [PubMed] [Europe PMC] [Abstract] Cited for: DNA-BINDING DOMAIN.
[6]	"Coupling of cell identity to signal response in yeast: interaction between the alpha-1 and STE12 proteins." Yuan Y.-L.O., Stroke I., Fields S. Genes Dev. 7:1584-1597(1993) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ALPHA1.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-400, MASS SPECTROMETRY. Strain: YAL6B.
[9]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-29; SER-226 AND SER-400, MASS SPECTROMETRY. Strain: ADR376.
[10]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-218 AND SER-226, MASS SPECTROMETRY.
[11]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-226; THR-289 AND SER-400, MASS SPECTROMETRY.
[12]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-29; SER-223; SER-226; SER-261; THR-289 AND SER-400, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X16112 Genomic DNA. Translation: CAA34246.1. M24502 Genomic DNA. Translation: AAA35109.1. U10556 Genomic DNA. Translation: AAB68884.1. BK006934 Genomic DNA. Translation: DAA06779.1.
PIR	A33540.
RefSeq	NP_011952.1. NM_001179214.1.
3D structure databases
ProteinModelPortal	P13574.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-64N.
IntAct	P13574. 23 interactions.
MINT	MINT-405050.
STRING	4932.YHR084W.
Proteomic databases
PaxDb	P13574.
PeptideAtlas	P13574.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR084W; YHR084W; YHR084W.
GeneID	856484.
KEGG	sce:YHR084W.
Organism-specific databases
CYGD	YHR084w.
SGD	S000001126. STE12.
Phylogenomic databases
eggNOG	NOG80751.
HOGENOM	HOG000000882.
KO	K11215.
OrthoDB	EOG42Z7Z9.
Gene expression databases
Genevestigator	P13574.
GermOnline	YHR084W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003120. TF_STE. [Graphical view]
Pfam	PF02200. STE. 1 hit. [Graphical view]
ProDom	PD018365. TF_STE. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00424. STE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982176.

Entry information

Entry name

STE12_YEAST

Accession

Primary (citable) accession number: P13574
Secondary accession number(s): D3DL35

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	April 3, 2013
This is version 124 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents