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P13574 (STE12_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 136. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein STE12
Gene names
Name:STE12
Ordered Locus Names:YHR084W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length688 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds to the DNA sequence mediating pheromone induction (called the pheromone response element = PRE) which is found in the upstream control region of several a-, alpha- and haploid-specific genes. Involved in mating of haploids and in pseudohyphae formation in diploids.

Subunit structure

Interacts with mating-type protein ALPHA1. Ref.6

Subcellular location

Nucleus.

Domain

The DNA-binding domain seems to be involved in the suppression of pseudohyphae formation under nitrogen-rich conditions and in haploids. This region is also involved in the regulation of budding pattern of haploids. Ref.5

Post-translational modification

Phosphorylated by the STE7, STE11 and STE20 kinases Probable.

Miscellaneous

Present with 1920 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the STE12 transcription factor family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 688688Protein STE12
PRO_0000072262

Regions

DNA binding57 – 167111 Ref.5

Amino acid modifications

Modified residue291Phosphothreonine Ref.8 Ref.9
Modified residue2141Phosphoserine Ref.10
Modified residue2261Phosphoserine Ref.8 Ref.9 Ref.10
Modified residue2891Phosphothreonine Ref.9
Modified residue4001Phosphoserine Ref.8 Ref.10

Sequences

Sequence LengthMass (Da)Tools
P13574 [UniParc].

Last modified January 1, 1990. Version 1.
Checksum: EE7371F18BBC0337

FASTA68877,867
        10         20         30         40         50         60 
MKVQITNSRT EEILKVQANN ENDEVSKATP GEVEESLRLI GDLKFFLATA PVNWQENQII 

        70         80         90        100        110        120 
RRYYLNSGQG FVSCVFWNNL YYITGTDIVK CCLYRMQKFG REVVQKKKFE EGIFSDLRNL 

       130        140        150        160        170        180 
KCGIDATLEQ PKSEFLSFLF RNMCLKTQKK QKVFFWFSVA HDKLFADALE RDLKRESLNQ 

       190        200        210        220        230        240 
PSTTKPVNEP ALSFSYDSSS DKPLYDQLLQ HLDSRRPSST TKSDNSPPKL ESENFKDNEL 

       250        260        270        280        290        300 
VTVTNQPLLG VGLMDDDAPE SPSQINDFIP QKLIIEPNTL ELNGLTEETP HDLPKNTAKG 

       310        320        330        340        350        360 
RDEEDFPLDY FPVSVEYPTE ENAFDPFPPQ AFTPAAPSMP ISYDNVNERD SMPVNSLLNR 

       370        380        390        400        410        420 
YPYQLSVAPT FPVPPSSSRQ HFMTNRDFYS SNNNKEKLVS PSDPTSYMKY DEPVMDFDES 

       430        440        450        460        470        480 
RPNENCTNAK SHNSGQQTKQ HQLYSNNFQQ SYPNGMVPGY YPKMPYNPMG GDPLLDQAFY 

       490        500        510        520        530        540 
GADDFFFPPE GCDNNMLYPQ TATSWNVLPP QAMQPAPTYV GRPYTPNYRS TPGSAMFPYM 

       550        560        570        580        590        600 
QSSNSMQWNT AVSPYSSRAP STTAKNYPPS TFYSQNINQY PRRRTVGMKS SQGNVPTGNK 

       610        620        630        640        650        660 
QSVGKSAKIS KPLHIKTSAY QKQYKINLET KARPSAGDED SAHPDKNKEI SMPTPDSNTL 

       670        680 
VVQSEEGGAH SLEVDTNRRS DKNLPDAT 

« Hide

References

« Hide 'large scale' references
[1]"The yeast STE12 protein binds to the DNA sequence mediating pheromone induction."
Dolan J.W., Kirkman C., Fields S.
Proc. Natl. Acad. Sci. U.S.A. 86:5703-5707(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"STE12, a protein involved in cell-type-specific transcription and signal transduction in yeast, is part of protein-DNA complexes."
Errede B., Ammerer G.
Genes Dev. 3:1349-1361(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII."
Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. expand/collapse author list , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M.
Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Properties of the DNA-binding domain of the Saccharomyces cerevisiae STE12 protein."
Yuan Y.-L., Fields S.
Mol. Cell. Biol. 11:5910-5918(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: DNA-BINDING DOMAIN.
[6]"Coupling of cell identity to signal response in yeast: interaction between the alpha-1 and STE12 proteins."
Yuan Y.-L.O., Stroke I., Fields S.
Genes Dev. 7:1584-1597(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ALPHA1.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-29; SER-226 AND SER-400, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-29; SER-226 AND THR-289, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-214; SER-226 AND SER-400, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X16112 Genomic DNA. Translation: CAA34246.1.
M24502 Genomic DNA. Translation: AAA35109.1.
U10556 Genomic DNA. Translation: AAB68884.1.
BK006934 Genomic DNA. Translation: DAA06779.1.
PIRA33540.
RefSeqNP_011952.1. NM_001179214.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3W3WX-ray2.20B581-649[»]
ProteinModelPortalP13574.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36519. 94 interactions.
DIPDIP-64N.
IntActP13574. 23 interactions.
MINTMINT-405050.
STRING4932.YHR084W.

Proteomic databases

MaxQBP13574.
PaxDbP13574.
PeptideAtlasP13574.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYHR084W; YHR084W; YHR084W.
GeneID856484.
KEGGsce:YHR084W.

Organism-specific databases

CYGDYHR084w.
SGDS000001126. STE12.

Phylogenomic databases

eggNOGNOG80751.
HOGENOMHOG000000882.
KOK11215.
OMACKWTSAN.
OrthoDBEOG7J44MH.

Enzyme and pathway databases

BioCycYEAST:G3O-31131-MONOMER.

Gene expression databases

GenevestigatorP13574.

Family and domain databases

InterProIPR003120. TF_STE.
[Graphical view]
PfamPF02200. STE. 1 hit.
[Graphical view]
ProDomPD018365. TF_STE. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00424. STE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio982176.

Entry information

Entry nameSTE12_YEAST
AccessionPrimary (citable) accession number: P13574
Secondary accession number(s): D3DL35
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: July 9, 2014
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references