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P13574

- STE12_YEAST

UniProt

P13574 - STE12_YEAST

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Protein

Protein STE12

Gene

STE12

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Binds to the DNA sequence mediating pheromone induction (called the pheromone response element = PRE) which is found in the upstream control region of several a-, alpha- and haploid-specific genes. Involved in mating of haploids and in pseudohyphae formation in diploids.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi57 – 167111Add
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. sequence-specific DNA binding transcription factor activity Source: SGD

GO - Biological processi

  1. conjugation with cellular fusion Source: SGD
  2. invasive growth in response to glucose limitation Source: SGD
  3. negative regulation of transcription from RNA polymerase II promoter by pheromones Source: SGD
  4. positive regulation of transcription from RNA polymerase II promoter by pheromones Source: SGD
  5. pseudohyphal growth Source: SGD
  6. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Pheromone response, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31131-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein STE12
Gene namesi
Name:STE12
Ordered Locus Names:YHR084W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VIII

Organism-specific databases

CYGDiYHR084w.
SGDiS000001126. STE12.

Subcellular locationi

GO - Cellular componenti

  1. nucleoplasm Source: SGD
  2. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 688688Protein STE12PRO_0000072262Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei29 – 291Phosphothreonine2 Publications
Modified residuei214 – 2141Phosphoserine1 Publication
Modified residuei226 – 2261Phosphoserine3 Publications
Modified residuei289 – 2891Phosphothreonine1 Publication
Modified residuei400 – 4001Phosphoserine2 Publications

Post-translational modificationi

Phosphorylated by the STE7, STE11 and STE20 kinases.3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP13574.
PaxDbiP13574.
PeptideAtlasiP13574.

Expressioni

Gene expression databases

GenevestigatoriP13574.

Interactioni

Subunit structurei

Interacts with mating-type protein ALPHA1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
DIG1Q030633EBI-18264,EBI-29752
DIG2Q033733EBI-18264,EBI-34019
HMLALPHA1P013652EBI-18264,EBI-10438
KSS1P146816EBI-18264,EBI-9945
SLT2Q007722EBI-18264,EBI-17372

Protein-protein interaction databases

BioGridi36519. 94 interactions.
DIPiDIP-64N.
IntActiP13574. 23 interactions.
MINTiMINT-405050.
STRINGi4932.YHR084W.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3W3WX-ray2.20B581-649[»]
ProteinModelPortaliP13574.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The DNA-binding domain seems to be involved in the suppression of pseudohyphae formation under nitrogen-rich conditions and in haploids. This region is also involved in the regulation of budding pattern of haploids.

Sequence similaritiesi

Belongs to the STE12 transcription factor family.Curated

Phylogenomic databases

eggNOGiNOG80751.
HOGENOMiHOG000000882.
InParanoidiP13574.
KOiK11215.
OMAiCKWTSAN.
OrthoDBiEOG7J44MH.

Family and domain databases

InterProiIPR003120. TF_STE.
[Graphical view]
PfamiPF02200. STE. 1 hit.
[Graphical view]
ProDomiPD018365. TF_STE. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00424. STE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P13574-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKVQITNSRT EEILKVQANN ENDEVSKATP GEVEESLRLI GDLKFFLATA
60 70 80 90 100
PVNWQENQII RRYYLNSGQG FVSCVFWNNL YYITGTDIVK CCLYRMQKFG
110 120 130 140 150
REVVQKKKFE EGIFSDLRNL KCGIDATLEQ PKSEFLSFLF RNMCLKTQKK
160 170 180 190 200
QKVFFWFSVA HDKLFADALE RDLKRESLNQ PSTTKPVNEP ALSFSYDSSS
210 220 230 240 250
DKPLYDQLLQ HLDSRRPSST TKSDNSPPKL ESENFKDNEL VTVTNQPLLG
260 270 280 290 300
VGLMDDDAPE SPSQINDFIP QKLIIEPNTL ELNGLTEETP HDLPKNTAKG
310 320 330 340 350
RDEEDFPLDY FPVSVEYPTE ENAFDPFPPQ AFTPAAPSMP ISYDNVNERD
360 370 380 390 400
SMPVNSLLNR YPYQLSVAPT FPVPPSSSRQ HFMTNRDFYS SNNNKEKLVS
410 420 430 440 450
PSDPTSYMKY DEPVMDFDES RPNENCTNAK SHNSGQQTKQ HQLYSNNFQQ
460 470 480 490 500
SYPNGMVPGY YPKMPYNPMG GDPLLDQAFY GADDFFFPPE GCDNNMLYPQ
510 520 530 540 550
TATSWNVLPP QAMQPAPTYV GRPYTPNYRS TPGSAMFPYM QSSNSMQWNT
560 570 580 590 600
AVSPYSSRAP STTAKNYPPS TFYSQNINQY PRRRTVGMKS SQGNVPTGNK
610 620 630 640 650
QSVGKSAKIS KPLHIKTSAY QKQYKINLET KARPSAGDED SAHPDKNKEI
660 670 680
SMPTPDSNTL VVQSEEGGAH SLEVDTNRRS DKNLPDAT
Length:688
Mass (Da):77,867
Last modified:January 1, 1990 - v1
Checksum:iEE7371F18BBC0337
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X16112 Genomic DNA. Translation: CAA34246.1.
M24502 Genomic DNA. Translation: AAA35109.1.
U10556 Genomic DNA. Translation: AAB68884.1.
BK006934 Genomic DNA. Translation: DAA06779.1.
PIRiA33540.
RefSeqiNP_011952.1. NM_001179214.1.

Genome annotation databases

EnsemblFungiiYHR084W; YHR084W; YHR084W.
GeneIDi856484.
KEGGisce:YHR084W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X16112 Genomic DNA. Translation: CAA34246.1 .
M24502 Genomic DNA. Translation: AAA35109.1 .
U10556 Genomic DNA. Translation: AAB68884.1 .
BK006934 Genomic DNA. Translation: DAA06779.1 .
PIRi A33540.
RefSeqi NP_011952.1. NM_001179214.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3W3W X-ray 2.20 B 581-649 [» ]
ProteinModelPortali P13574.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36519. 94 interactions.
DIPi DIP-64N.
IntActi P13574. 23 interactions.
MINTi MINT-405050.
STRINGi 4932.YHR084W.

Proteomic databases

MaxQBi P13574.
PaxDbi P13574.
PeptideAtlasi P13574.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YHR084W ; YHR084W ; YHR084W .
GeneIDi 856484.
KEGGi sce:YHR084W.

Organism-specific databases

CYGDi YHR084w.
SGDi S000001126. STE12.

Phylogenomic databases

eggNOGi NOG80751.
HOGENOMi HOG000000882.
InParanoidi P13574.
KOi K11215.
OMAi CKWTSAN.
OrthoDBi EOG7J44MH.

Enzyme and pathway databases

BioCyci YEAST:G3O-31131-MONOMER.

Miscellaneous databases

NextBioi 982176.

Gene expression databases

Genevestigatori P13574.

Family and domain databases

InterProi IPR003120. TF_STE.
[Graphical view ]
Pfami PF02200. STE. 1 hit.
[Graphical view ]
ProDomi PD018365. TF_STE. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SMARTi SM00424. STE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The yeast STE12 protein binds to the DNA sequence mediating pheromone induction."
    Dolan J.W., Kirkman C., Fields S.
    Proc. Natl. Acad. Sci. U.S.A. 86:5703-5707(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "STE12, a protein involved in cell-type-specific transcription and signal transduction in yeast, is part of protein-DNA complexes."
    Errede B., Ammerer G.
    Genes Dev. 3:1349-1361(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Properties of the DNA-binding domain of the Saccharomyces cerevisiae STE12 protein."
    Yuan Y.-L., Fields S.
    Mol. Cell. Biol. 11:5910-5918(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: DNA-BINDING DOMAIN.
  6. "Coupling of cell identity to signal response in yeast: interaction between the alpha-1 and STE12 proteins."
    Yuan Y.-L.O., Stroke I., Fields S.
    Genes Dev. 7:1584-1597(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ALPHA1.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-29; SER-226 AND SER-400, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-29; SER-226 AND THR-289, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-214; SER-226 AND SER-400, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSTE12_YEAST
AccessioniPrimary (citable) accession number: P13574
Secondary accession number(s): D3DL35
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: October 29, 2014
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1920 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3