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Reviewed, UniProtKB/Swiss-Prot P13568 (MDR_PLAFF)

Last modified October 13, 2009. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Multidrug resistance protein
    EC=3.6.3.44
Alternative name(s):
    Chloroquine resistance protein
Gene names
Name: MDR1
OrganismPlasmodium falciparum (isolate FC27 / Papua New Guinea)
Taxonomic identifier5837 [NCBI]
Taxonomic lineageEukaryota › Alveolata › Apicomplexa › Aconoidasida › Haemosporida › Plasmodium › Plasmodium (Laverania)

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Protein attributes

Sequence length1419 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.

Catalytic activity

ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).

Subcellular location

Membrane; Multi-pass membrane protein.

Miscellaneous

P.falciparum resistant to the drug chloroquine have multiple copies of the gene coding for MDR.

Sequence similarities

Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. [View classification]

Contains 2 ABC transmembrane type-1 domains.

Contains 2 ABC transporter domains.

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Ontologies

Keywords
   Biological processTransport
   Cellular componentMembrane
   DomainRepeat
Transmembrane
   LigandATP-binding
Nucleotide-binding
   Molecular functionHydrolase
   PTMGlycoprotein
Gene Ontology (GO)
   Biological processtransport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

xenobiotic-transporting ATPase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 14191419Multidrug resistance protein
PRO_0000093344

Regions

Topological domain1 – 5555Cytoplasmic Potential
Transmembrane56 – 8227 Potential
Transmembrane91 – 11626 Potential
Transmembrane160 – 18829 Potential
Transmembrane194 – 21219 Potential
Transmembrane279 – 29820 Potential
Transmembrane314 – 33825 Potential
Topological domain339 – 788450Cytoplasmic Potential
Transmembrane789 – 80719 Potential
Transmembrane825 – 84622 Potential
Transmembrane908 – 92821 Potential
Transmembrane1028 – 104821 Potential
Transmembrane1063 – 108321 Potential
Topological domain1084 – 1419336Cytoplasmic Potential
Domain57 – 345289ABC transmembrane type-1 1
Domain378 – 662285ABC transporter 1
Domain789 – 1089301ABC transmembrane type-1 2
Domain1126 – 1416291ABC transporter 2
Nucleotide binding413 – 4208ATP 1 Potential
Nucleotide binding1161 – 11688ATP 2 Potential
Compositional bias643 – 66119Poly-Asn

Amino acid modifications

Glycosylation2281N-linked (GlcNAc...) Potential
Glycosylation2581N-linked (GlcNAc...) Potential
Glycosylation9641N-linked (GlcNAc...) Potential

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Sequences

Sequence LengthMass (Da)Tools
P13568-1 [UniParc].

Last modified January 1, 1990. Version 1.
Checksum: 0F96C7C1850B33D0

FASTA1,419162,252
        10         20         30         40         50         60 
MGKEQKEKKD GNLSIKEEVE KELNKKSTAE LFRKIKNEKI SFFLPFKCLP AQHRKLLFIS 

        70         80         90        100        110        120 
FVCAVLSGGT LPFFISVFGV ILKNMNLGDD INPIILSLVS IGLVQFILSM ISSYCMDVIT 

       130        140        150        160        170        180 
SKILKTLKLE YLRSVFYQDG QFHDNNPGSK LRSDLDFYLE QVSSGIGTKF ITIFTYASSF 

       190        200        210        220        230        240 
LGLYIWSLIK NARLTLCITC VFPLIYVCGV ICNKKVKLNK KTSLLYNNNT MSIIEEALMG 

       250        260        270        280        290        300 
IRTVASYCGE KTILNKFNLS ETFYSKYILK ANFVEALHIG LINGLILVSY AFGFWYGTRI 

       310        320        330        340        350        360 
IINSATNQYP NNDFNGASVI SILLGVLISM FMLTIILPNI TEYMKALEAT NSLYEIINRK 

       370        380        390        400        410        420 
PLVENNDDGE TLPNIKKIEF KNVRFHYDTR KDVEIYKDLS FTLKEGKTYA FVGESGCGKS 

       430        440        450        460        470        480 
TILKLIERLY DPTEGDIIVN DSHNLKDINL KWWRSKIGVV SQDPLLFSNS IKNNIKYSLY 

       490        500        510        520        530        540 
SLKDLEAMEN YYEENTNDTY ENKNFSLISN SMTSNELLEM KKEYQTIKDS DVVDVSKKVL 

       550        560        570        580        590        600 
IHDFVSSLPD KYDTLVGSNA SKLSGGQKQR ISIARAIMRN PKILILDEAT SSLDNKSEYL 

       610        620        630        640        650        660 
VQKTINNLKG NENRITIIIA HRLSTIRYAN TIFVLSNRER SDNNNNNNND DNNNNNNNNN 

       670        680        690        700        710        720 
NKINNEGSYI IEQGTHDSLM KNKNGIYHLM INNQKISSNK SSNNGNDNGS DNKSSAYKDS 

       730        740        750        760        770        780 
DTGNDADNMN SLSIHENENI SNNRNCKNTA ENEKEEKVPF FKRMFRRKKK APNNLRIIYK 

       790        800        810        820        830        840 
EIFSYKKDVT IIFFSILVAG GLYPVFALLY ARYVSTLFDF ANLEYNSNKY SIYILLIAIA 

       850        860        870        880        890        900 
MFISETLKNY YNNKIGEKVE KTMKRRLFEN ILYQEMSFFD QDKNTPGVLS AHINRDVHLL 

       910        920        930        940        950        960 
KTGLVNNIVI FSHFIMLFLV SMVMSFYFCP IVAAVLTFIY FINMRVFAVR ARLTKSKEIE 

       970        980        990       1000       1010       1020 
KKENMSSGVF AFSSDDEMFK DPSFLIQEAF YNMHTVINYG LEDYFCNLIE KAIDYKNKGQ 

      1030       1040       1050       1060       1070       1080 
KRRIIVNAAL WGFSQSAQLF INSFAYWFGS FLIKRGTILV DDFMKSLFTF IFTGSYAGKL 

      1090       1100       1110       1120       1130       1140 
MSLKGDSENA KLSFEKYYPL MIRKSNIDVR DDGGIRINKN LIKGKVDIKD VNFRYISRPN 

      1150       1160       1170       1180       1190       1200 
VPIYKNLSFT CDSKKTTAIV GETGSGKSTF MNLLLRFYDL KNDHIILKND MTNFQDYQNN 

      1210       1220       1230       1240       1250       1260 
NNNSLVLKNV NEFSNQSGSA EDYTVFNNNG EILLDDINIC DYNLRDLRNL FSIVSQEPML 

      1270       1280       1290       1300       1310       1320 
FNMSIYENIK FGREDATLED VKRVSKFAAI DEFIESLPNK YDTNVGPYGK SLSGGQKQRI 

      1330       1340       1350       1360       1370       1380 
AIARALLREP KILLLDEATS SLDSNSEKLI EKTIVDIKDK ADKTIITIAH RIASIKRSDK 

      1390       1400       1410 
IVVFNNPDRN GTFVQSHGTH DELLSAQDGI YKKYVKLAK

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References

[1]"Amplification of the multidrug resistance gene in some chloroquine-resistant isolates of P. falciparum."
Foote S.J., Thompson J.K., Cowman A.F., Kemp D.J.
Cell 57:921-930(1989) [PubMed: 2701941] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Amplification of the multidrug resistance gene pfmdr1 in Plasmodium falciparum has arisen as multiple independent events."
Triglia T., Foote S.J., Kemp D.J., Cowman A.F.
Mol. Cell. Biol. 11:5244-5250(1991) [PubMed: 1922044] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Amplification of pfmdr 1 associated with mefloquine and halofantrine resistance in Plasmodium falciparum from Thailand."
Wilson C.M., Volkman S.K., Thaithong S., Martin R.K., Kyle D.E., Milhous W.K., Wirth D.F.
Mol. Biochem. Parasitol. 57:151-160(1993) [PubMed: 8426608] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

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Cross-references

Sequence databases

M29154 Genomic DNA. Translation: AAA29646.1.
X56851 Genomic DNA. Translation: CAA40180.1.
S53996 Genomic DNA. Translation: AAD13870.1.
PIRDVZQF. S18204.

3D structure databases

HSSPHSSP built from PDB template 1JJ7 based on UniProtKB Q03518.
ModBaseSearch...

Protein family/group databases

TCDB3.A.1.201.4. ATP-binding cassette (ABC) superfamily.

Family and domain databases

InterProIPR001140. ABC_TM_transpt.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR017940. ABC_transporter_type1.
IPR003593. ATPase_AAA+_core.
[Graphical view]
PfamPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
ProDomPD000006. ABC_transporter. 2 hits.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00382. AAA. 2 hits.
[Graphical view]
PROSITEPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameMDR_PLAFF
AccessionPrimary (citable) accession number: P13568
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: October 13, 2009
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents