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Protein

Glutamine synthetase leaf isozyme, chloroplastic

Gene
N/A
Organism
Hordeum vulgare (Barley)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

The light-modulated chloroplast enzyme, encoded by a nuclear gene and expressed primarily in leaves, is responsible for the reassimilation of the ammonia generated by photorespiration.

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.3.1.2. 2687.
SABIO-RKP13564.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase leaf isozyme, chloroplastic (EC:6.3.1.2)
Alternative name(s):
GS2
Glutamate--ammonia ligase
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaeTriticodaeTriticeaeHordeinaeHordeum

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 54ChloroplastAdd BLAST54
ChainiPRO_000001117855 – 434Glutamine synthetase leaf isozyme, chloroplasticAdd BLAST380

Proteomic databases

PRIDEiP13564.

Interactioni

Subunit structurei

Homooctamer.

Structurei

3D structure databases

ProteinModelPortaliP13564.
SMRiP13564.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SMARTiSM01230. Gln-synt_C. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P13564-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQVRRDDDGA GGCAGDAVPG GGEGQDGVPA RQPAGRVWGV SRAARATSGF
60 70 80 90 100
KVLALGPETT GVIQRMQQLL DMDTTPFTDK IIAEYIWVGG SGIDLRSKSR
110 120 130 140 150
TISKPVEDPS ELPKWNYDGS STGQAPGEDS EVILYPQAIF KDPFRGGNNI
160 170 180 190 200
LVICDTYTPQ GEPIPTNKRH MAAQIFSDPK VTSQVPWFGI EQEYTLMQRD
210 220 230 240 250
VNWPLGWPVG GYPGPQGPYY CAVGSDKSFG RDISDAHYKA CLYAGIEISG
260 270 280 290 300
TNGEVMPGQW EYQVGPSVGI DAGDHIWASR YILERITEQA GVVLTLDPKP
310 320 330 340 350
IQGDWNGAGC HTNYSTLSMR EDGGFDVIKK AILNLSLRHD LHIAAYGEGN
360 370 380 390 400
ERRLTGLHET ASISDFSWGV ANRGCSIRVG RDTEAKGKGY LEDRRPASNM
410 420 430
DPYTVTALLA ETTILWEPTL EAEALAAKKL ALKV
Length:434
Mass (Da):47,094
Last modified:November 1, 1990 - v2
Checksum:iFC47F5685EFC0D1E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti9 – 27GAGGC…EGQDG → AQAVVQAMQCQVGVRGRTA in CAA34131 (PubMed:1983286).CuratedAdd BLAST19
Sequence conflicti41S → R in CAA34131 (PubMed:1983286).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53580 mRNA. Translation: CAA37643.1.
X16000 mRNA. Translation: CAA34131.1.
PIRiS11865. AJBHQ.
UniGeneiHv.733.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53580 mRNA. Translation: CAA37643.1.
X16000 mRNA. Translation: CAA34131.1.
PIRiS11865. AJBHQ.
UniGeneiHv.733.

3D structure databases

ProteinModelPortaliP13564.
SMRiP13564.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP13564.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi6.3.1.2. 2687.
SABIO-RKP13564.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SMARTiSM01230. Gln-synt_C. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLNA2_HORVU
AccessioniPrimary (citable) accession number: P13564
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: November 1, 1990
Last modified: November 30, 2016
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

In barley, there are distinct isozymes in the chloroplast, and cytoplasm.

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.