P13563 (HAS1_XENLA) Reviewed, UniProtKB/Swiss-Prot
Last modified
November 16, 2011.
Version 74.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Hyaluronan synthase 1 EC=2.4.1.212 Alternative name(s): DG42 protein Hyaluronate synthase 1 Hyaluronic acid synthase 1 Short name=HA synthase 1 Short name=xHAS1 | ||
| Gene names |
| ||
| Organism | Xenopus laevis (African clawed frog) | ||
| Taxonomic identifier | 8355 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus |
Protein attributes
| Sequence length | 588 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Plays a role in hyaluronan/hyaluronic acid (HA) synthesis. May play a role in signaling or pattern formation in embryonic development. Ref.1 Ref.2 |
| Catalytic activity | UDP-alpha-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan) = UDP + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan). Ref.2 UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan) = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan). Ref.2 |
| Cofactor | Magnesium. |
| Pathway | |
| Subcellular location | Membrane; Multi-pass membrane protein Probable. |
| Tissue specificity | Expression moves as a gradient through the embryo. The mRNA is first expressed in the animal region of the blastula, and by early gastrula is found everywhere except in the outer layer of the dorsal blastopore lip. By mid-gastrula, protein is present in the inner ectodermal layer and the endoderm, then disappears from dorsal ectoderm as the neural plate is induced and later decays in a dorsoventral direction. Last expressed in ventral regions of the gut at the tailbud stage (at protein level). Ref.1 |
| Developmental stage | Expressed during a short window of embryogenesis. Expression of mRNA begins just after mid-blastula, peaks at late gastrula, and declines by the end of neurulation. Protein expression follows that of the mRNA with a time lag of approximately 2 hours: accumulates through gastrula and early neurula stages and peaks at about stage 18 (mid-neurula), then decays in a non-uniform manner, persisting about 12 to 18 hr longer than the RNA (at protein level). Ref.1 Ref.2 |
| Sequence similarities | Belongs to the nodC/HAS family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Membrane |
| Domain | Transmembrane Transmembrane helix |
| Molecular function | Glycosyltransferase Transferase |
| Gene Ontology (GO) | |
| Cellular component | integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | hyaluronan synthase activity Inferred from direct assay Ref.2. Source: UniProtKB |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 588 | 588 | Hyaluronan synthase 1 | PRO_0000197171 | |||||
Regions | |||||||||
| Topological domain | 1 – 28 | 28 | Cytoplasmic Potential | ||||||
| Transmembrane | 29 – 49 | 21 | Helical; Name=1; Potential | ||||||
| Topological domain | 50 – 61 | 12 | Extracellular Potential | ||||||
| Transmembrane | 62 – 82 | 21 | Helical; Name=2; Potential | ||||||
| Topological domain | 83 – 411 | 329 | Cytoplasmic Potential | ||||||
| Transmembrane | 412 – 432 | 21 | Helical; Name=3; Potential | ||||||
| Topological domain | 433 | 1 | Extracellular Potential | ||||||
| Transmembrane | 434 – 454 | 21 | Helical; Name=4; Potential | ||||||
| Topological domain | 455 – 456 | 2 | Cytoplasmic Potential | ||||||
| Transmembrane | 457 – 477 | 21 | Helical; Name=5; Potential | ||||||
| Topological domain | 478 – 505 | 28 | Extracellular Potential | ||||||
| Transmembrane | 506 – 526 | 21 | Helical; Name=6; Potential | ||||||
| Topological domain | 527 – 543 | 17 | Cytoplasmic Potential | ||||||
| Transmembrane | 544 – 564 | 21 | Helical; Name=7; Potential | ||||||
| Topological domain | 565 – 588 | 24 | Extracellular Potential | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Accumulation and decay of DG42 gene products follow a gradient pattern during Xenopus embryogenesis." Rosa F., Sargent T.D., Rebbert M.L., Michaels G.S., Jamrich M., Grunz H., Jonas E., Winkles J.A., Dawid I.B. Dev. Biol. 129:114-123(1988) [PubMed: 3410156] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Tissue: Gastrula. |
| [2] | "Characterization and molecular evolution of a vertebrate hyaluronan synthase gene family." Spicer A.P., McDonald J.A. J. Biol. Chem. 273:1923-1932(1998) [PubMed: 9442026] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, DEVELOPMENTAL STAGE. |
| [3] | NIH - Xenopus Gene Collection (XGC) project Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Neurula. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | M22249 mRNA. Translation: AAA49699.1. BC047963 mRNA. Translation: AAH47963.1. BC108480 mRNA. Translation: AAI08481.1. |
| PIR | A43740. |
| RefSeq | NP_001079696.1. NM_001086227.1. |
| UniGene | Xl.4517. |
3D structure databases | |
| ProteinModelPortal | P13563. |
| ModBase | Search... |
Protein family/group databases | |
| CAZy | GT2. Glycosyltransferase Family 2. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 379383. |
| KEGG | xla:379383. |
Organism-specific databases | |
| CTD | 3036. |
| Xenbase | XB-GENE-6256069. has1. |
Phylogenomic databases | |
| HOVERGEN | HBG000189. |
Family and domain databases | |
| InterPro | IPR004835. Chitin_synth_fng. [Graphical view] |
| KO | K00752. |
| Pfam | PF03142. Chitin_synth_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | HAS1_XENLA | ||||||||
| Accession | Primary (citable) accession number: P13563 Secondary accession number(s): Q5D0A1 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with