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Protein

Hyaluronan synthase 1

Gene

has1

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer. Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. Also able to catalyze the synthesis of chito-oligosaccharide depending on the substrate.2 Publications

Catalytic activityi

UDP-alpha-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan) = UDP + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan).1 Publication
UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan) = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan).1 Publication

Cofactori

Pathwayi: hyaluronan biosynthesis

This protein is involved in the pathway hyaluronan biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway hyaluronan biosynthesis and in Glycan biosynthesis.

GO - Molecular functioni

  • hyaluronan synthase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00341.

Protein family/group databases

CAZyiGT2. Glycosyltransferase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Hyaluronan synthase 1 (EC:2.4.1.212)
Alternative name(s):
DG42 protein
Hyaluronate synthase 1
Hyaluronic acid synthase 1
Short name:
HA synthase 1
Short name:
xHAS1
Gene namesi
Name:has1
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-6256069. has1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2828CytoplasmicSequence analysisAdd
BLAST
Transmembranei29 – 4921Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini50 – 6112ExtracellularSequence analysisAdd
BLAST
Transmembranei62 – 8221Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini83 – 411329CytoplasmicSequence analysisAdd
BLAST
Transmembranei412 – 43221Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini433 – 4331ExtracellularSequence analysis
Transmembranei434 – 45421Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini455 – 4562CytoplasmicSequence analysis
Transmembranei457 – 47721Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini478 – 50528ExtracellularSequence analysisAdd
BLAST
Transmembranei506 – 52621Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini527 – 54317CytoplasmicSequence analysisAdd
BLAST
Transmembranei544 – 56421Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini565 – 58824ExtracellularSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 588588Hyaluronan synthase 1PRO_0000197171Add
BLAST

Proteomic databases

PRIDEiP13563.

Expressioni

Tissue specificityi

Expression moves as a gradient through the embryo. The mRNA is first expressed in the animal region of the blastula, and by early gastrula is found everywhere except in the outer layer of the dorsal blastopore lip. By mid-gastrula, protein is present in the inner ectodermal layer and the endoderm, then disappears from dorsal ectoderm as the neural plate is induced and later decays in a dorsoventral direction. Last expressed in ventral regions of the gut at the tailbud stage (at protein level).1 Publication

Developmental stagei

Expressed during a short window of embryogenesis. Expression of mRNA begins just after mid-blastula, peaks at late gastrula, and declines by the end of neurulation. Protein expression follows that of the mRNA with a time lag of approximately 2 hours: accumulates through gastrula and early neurula stages and peaks at about stage 18 (mid-neurula), then decays in a non-uniform manner, persisting about 12 to 18 hr longer than the RNA (at protein level).2 Publications

Structurei

3D structure databases

ProteinModelPortaliP13563.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NodC/HAS family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG000189.
KOiK00752.

Family and domain databases

Gene3Di3.90.550.10. 2 hits.
InterProiIPR026107. HAS/NodC.
IPR028385. HAS1.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR22913. PTHR22913. 1 hit.
PTHR22913:SF4. PTHR22913:SF4. 1 hit.
SUPFAMiSSF53448. SSF53448. 2 hits.

Sequencei

Sequence statusi: Complete.

P13563-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKEKAAETME IPEGIPKDLE PKHPTLWRII YYSFGVVLLA TITAAYVAEF
60 70 80 90 100
QVLKHEAILF SLGLYGLAML LHLMMQSLFA FLEIRRVNKS ELPCSFKKTV
110 120 130 140 150
ALTIAGYQEN PEYLIKCLES CKYVKYPKDK LKIILVIDGN TEDDAYMMEM
160 170 180 190 200
FKDVFHGEDV GTYVWKGNYH TVKKPEETNK GSCPEVSKPL NEDEGINMVE
210 220 230 240 250
ELVRNKRCVC IMQQWGGKRE VMYTAFQAIG TSVDYVQVCD SDTKLDELAT
260 270 280 290 300
VEMVKVLESN DMYGAVGGDV RILNPYDSFI SFMSSLRYWM AFNVERACQS
310 320 330 340 350
YFDCVSCISG PLGMYRNNIL QVFLEAWYRQ KFLGTYCTLG DDRHLTNRVL
360 370 380 390 400
SMGYRTKYTH KSRAFSETPS LYLRWLNQQT RWTKSYFREW LYNAQWWHKH
410 420 430 440 450
HIWMTYESVV SFIFPFFITA TVIRLIYAGT IWNVVWLLLC IQIMSLFKSI
460 470 480 490 500
YACWLRGNFI MLLMSLYSML YMTGLLPSKY FALLTLNKTG WGTSGRKKIV
510 520 530 540 550
GNYMPILPLS IWAAVLCGGV GYSIYMDCQN DWSTPEKQKE MYHLLYGCVG
560 570 580
YVMYWVIMAV MYWVWVKRCC RKRSQTVTLV HDIPDMCV
Length:588
Mass (Da):68,522
Last modified:January 1, 1990 - v1
Checksum:i33DA3B8E331F4CE9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22249 mRNA. Translation: AAA49699.1.
BC047963 mRNA. Translation: AAH47963.1.
BC108480 mRNA. Translation: AAI08481.1.
PIRiA43740.
RefSeqiNP_001079696.1. NM_001086227.1.
UniGeneiXl.4517.

Genome annotation databases

GeneIDi379383.
KEGGixla:379383.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22249 mRNA. Translation: AAA49699.1.
BC047963 mRNA. Translation: AAH47963.1.
BC108480 mRNA. Translation: AAI08481.1.
PIRiA43740.
RefSeqiNP_001079696.1. NM_001086227.1.
UniGeneiXl.4517.

3D structure databases

ProteinModelPortaliP13563.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT2. Glycosyltransferase Family 2.

Proteomic databases

PRIDEiP13563.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi379383.
KEGGixla:379383.

Organism-specific databases

CTDi3036.
XenbaseiXB-GENE-6256069. has1.

Phylogenomic databases

HOVERGENiHBG000189.
KOiK00752.

Enzyme and pathway databases

UniPathwayiUPA00341.

Family and domain databases

Gene3Di3.90.550.10. 2 hits.
InterProiIPR026107. HAS/NodC.
IPR028385. HAS1.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR22913. PTHR22913. 1 hit.
PTHR22913:SF4. PTHR22913:SF4. 1 hit.
SUPFAMiSSF53448. SSF53448. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiHYAS1_XENLA
AccessioniPrimary (citable) accession number: P13563
Secondary accession number(s): Q5D0A1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: November 26, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.