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P13559

- VLYS_BPPRD

UniProt

P13559 - VLYS_BPPRD

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Protein

Lysozyme

Gene

XV

Organism
Enterobacteria phage PRD1 (Bacteriophage PRD1)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Endolysin involved in host peptidoglycan digestion after permeabilization of the cytoplasmic membrane by holin. Involved in host cell lysis and liberation of progeny phages. Displays strong lytic activity against chloroform-treated Gram-negative cells.2 Publications

Catalytic activityi

Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.

pH dependencei

Optimum pH is 7.0-8.0.

Temperature dependencei

Inactivation temperature above 4 degrees Celsius.

GO - Molecular functioni

  1. lysozyme activity Source: CACAO

GO - Biological processi

  1. catabolism by organism of cell wall peptidoglycan in other organism Source: CACAO
  2. cytolysis Source: UniProtKB-KW
  3. defense response to bacterium Source: UniProtKB-KW
  4. viral release from host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Antimicrobial, Bacteriolytic enzyme, Glycosidase, Hydrolase

Keywords - Biological processi

Cytolysis, Host cell lysis by virus, Virus exit from host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Lysozyme (EC:3.2.1.17)
Alternative name(s):
Lytic enzyme
Muramidase
Protein P15
Gene namesi
Name:XV
OrganismiEnterobacteria phage PRD1 (Bacteriophage PRD1)
Taxonomic identifieri10658 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageTectiviridaeTectivirus
Virus hostiAcinetobacter calcoaceticus [TaxID: 471]
Escherichia coli [TaxID: 562]
Proteus mirabilis [TaxID: 584]
Pseudomonas aeruginosa [TaxID: 287]
Pseudomonas fluorescens [TaxID: 294]
Pseudomonas putida (Arthrobacter siderocapsulatus) [TaxID: 303]
Salmonella typhimurium [TaxID: 90371]
Vibrio cholerae [TaxID: 666]
ProteomesiUP000002143: Genome

Subcellular locationi

Virion membrane 1 Publication; Peripheral membrane protein 1 Publication
Note: May be associated with the viral membrane via P20 and P22.

GO - Cellular componenti

  1. virion membrane Source: CACAO
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 149149LysozymePRO_0000165348Add
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 24 family.Curated

Family and domain databases

InterProiIPR024408. DUF3380.
IPR016284. Phage_PRD1_P15_lysozyme.
[Graphical view]
PfamiPF11860. DUF3380. 1 hit.
[Graphical view]
PIRSFiPIRSF001069. Lytic_enz_p15. 1 hit.

Sequencei

Sequence statusi: Complete.

P13559-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQYTLWDIIS RVESNGNLKA LRFEPEYYQR RMERGDWDNS IIQNIRAANK
60 70 80 90 100
CSLGTARMIY CSSWGAVQIM GFNLYLNGAF NLSVAHFMEN EAYQVNEFRR
110 120 130 140
FLLKNGLTEY TPERLASDKA ARVKFAKVYN GAESYADLIL QACQFYGVK
Length:149
Mass (Da):17,269
Last modified:February 1, 1991 - v2
Checksum:i50ABB718032939B5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14980 Genomic DNA. Translation: CAA33104.1.
M22161 Genomic DNA. Translation: AAA32451.1.
AY848689 Genomic DNA. Translation: AAX45908.1.
PIRiS03568. LYBPD1.
RefSeqiNP_040683.1. NC_001421.2.

Genome annotation databases

GeneIDi1260935.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14980 Genomic DNA. Translation: CAA33104.1 .
M22161 Genomic DNA. Translation: AAA32451.1 .
AY848689 Genomic DNA. Translation: AAX45908.1 .
PIRi S03568. LYBPD1.
RefSeqi NP_040683.1. NC_001421.2.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1260935.

Family and domain databases

InterProi IPR024408. DUF3380.
IPR016284. Phage_PRD1_P15_lysozyme.
[Graphical view ]
Pfami PF11860. DUF3380. 1 hit.
[Graphical view ]
PIRSFi PIRSF001069. Lytic_enz_p15. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Comparison of the amino acid sequence of the lytic enzyme from broad-host-range bacteriophage PRD1 with sequences of other cell-wall-peptidoglycan lytic enzymes."
    Pakula T.M., Savilahti H., Bamford D.H.
    Eur. J. Biochem. 180:149-152(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The complete nucleotide sequence of the left very early region of Escherichia coli bacteriophage PRD1 coding for the terminal protein and the DNA polymerase."
    Savilahti H., Bamford D.H.
    Gene 57:121-130(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-98.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "A snapshot of viral evolution from genome analysis of the tectiviridae family."
    Saren A.M., Ravantti J.J., Benson S.D., Burnett R.M., Paulin L., Bamford D.H., Bamford J.K.H.
    J. Mol. Biol. 350:427-440(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. "Gene XV of bacteriophage PRD1 encodes a lytic enzyme with muramidase activity."
    Caldentey J., Hanninen A.L., Bamford D.H.
    Eur. J. Biochem. 225:341-346(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "The lytic enzyme of bacteriophage PRD1 is associated with the viral membrane."
    Rydman P.S., Bamford D.H.
    J. Bacteriol. 184:104-110(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiVLYS_BPPRD
AccessioniPrimary (citable) accession number: P13559
Secondary accession number(s): Q3T4P3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: February 1, 1991
Last modified: October 1, 2014
This is version 67 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3