P13559 (VLYS_BPPRD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lysozyme
EC=3.2.1.17

Alternative name(s):
Lytic enzyme
Muramidase
Protein P15

Gene names

Name:

XV

Organism

Enterobacteria phage PRD1 (Bacteriophage PRD1) [Reference proteome]

Taxonomic identifier

10658 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Tectiviridae › Tectivirus

Virus host

Acinetobacter calcoaceticus [TaxID: 471]
Escherichia coli [TaxID: 562]
Proteus mirabilis [TaxID: 584]
Pseudomonas aeruginosa [TaxID: 287]
Pseudomonas fluorescens [TaxID: 294]
Pseudomonas putida (Arthrobacter siderocapsulatus) [TaxID: 303]
Salmonella typhimurium [TaxID: 90371]
Vibrio cholerae [TaxID: 666]

Protein attributes

Sequence length	149 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Endolysin involved in host peptidoglycan digestion after permeabilization of the cytoplasmic membrane by holin. Involved in host cell lysis and liberation of progeny phages. Displays strong lytic activity against chloroform-treated Gram-negative cells. Ref.5 Ref.6
Catalytic activity	Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.
Subcellular location	Virion membrane; Peripheral membrane protein. Note: May be associated with the viral membrane via P20 and P22. Ref.6
Sequence similarities	Belongs to the glycosyl hydrolase 24 family.
Biophysicochemical properties	pH dependence: Optimum pH is 7.0-8.0. Temperature dependence: Inactivation temperature above 4 degrees Celsius.

Ontologies

Keywords
Cellular component	Membrane Virion
Molecular function	Antimicrobial Bacteriolytic enzyme Glycosidase Hydrolase Phage lysis protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: UniProtKB-KW cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	virion membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	lysozyme activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 149	149	Lysozyme		PRO_0000165348

Sequences

	Sequence		Length	Mass (Da)
	P13559 [UniParc]. Last modified February 1, 1991. Version 2. Checksum: 50ABB718032939B5	FASTA	149	17,269
	10 20 30 40 50 60 MQYTLWDIIS RVESNGNLKA LRFEPEYYQR RMERGDWDNS IIQNIRAANK CSLGTARMIY 70 80 90 100 110 120 CSSWGAVQIM GFNLYLNGAF NLSVAHFMEN EAYQVNEFRR FLLKNGLTEY TPERLASDKA 130 140 ARVKFAKVYN GAESYADLIL QACQFYGVK

References

[1]	"Comparison of the amino acid sequence of the lytic enzyme from broad-host-range bacteriophage PRD1 with sequences of other cell-wall-peptidoglycan lytic enzymes." Pakula T.M., Savilahti H., Bamford D.H. Eur. J. Biochem. 180:149-152(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete nucleotide sequence of the left very early region of Escherichia coli bacteriophage PRD1 coding for the terminal protein and the DNA polymerase." Savilahti H., Bamford D.H. Gene 57:121-130(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-98.
[3]	"Genome organization of membrane-containing bacteriophage PRD1." Bamford J.K.H., Haenninen A.-L., Pakula T.M., Ojala P.M., Kalkkinen N., Frilander M., Bamford D.H. Virology 183:658-676(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"A snapshot of viral evolution from genome analysis of the tectiviridae family." Saren A.M., Ravantti J.J., Benson S.D., Burnett R.M., Paulin L., Bamford D.H., Bamford J.K.H. J. Mol. Biol. 350:427-440(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"Gene XV of bacteriophage PRD1 encodes a lytic enzyme with muramidase activity." Caldentey J., Hanninen A.L., Bamford D.H. Eur. J. Biochem. 225:341-346(1994) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"The lytic enzyme of bacteriophage PRD1 is associated with the viral membrane." Rydman P.S., Bamford D.H. J. Bacteriol. 184:104-110(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X14980 Genomic DNA. Translation: CAA33104.1. M22161 Genomic DNA. Translation: AAA32451.1. AY848689 Genomic DNA. Translation: AAX45908.1.
PIR	LYBPD1. S03568.
RefSeq	NP_040683.1. NC_001421.2.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1260935.
Family and domain databases
InterPro	IPR024408. DUF3380. IPR016284. Phage_PRD1_P15_lysozyme. [Graphical view]
Pfam	PF11860. DUF3380. 1 hit. [Graphical view]
PIRSF	PIRSF001069. Lytic_enz_p15. 1 hit.
ProtoNet	Search...

Entry information

Entry name

VLYS_BPPRD

Accession

Primary (citable) accession number: P13559
Secondary accession number(s): Q3T4P3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	February 1, 1991
Last modified:	May 1, 2013
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program