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P13559

- VLYS_BPPRD

UniProt

P13559 - VLYS_BPPRD

Protein

Lysozyme

Gene

XV

Organism
Enterobacteria phage PRD1 (Bacteriophage PRD1)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Endolysin involved in host peptidoglycan digestion after permeabilization of the cytoplasmic membrane by holin. Involved in host cell lysis and liberation of progeny phages. Displays strong lytic activity against chloroform-treated Gram-negative cells.2 Publications

    Catalytic activityi

    Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.

    pH dependencei

    Optimum pH is 7.0-8.0.

    Temperature dependencei

    Inactivation temperature above 4 degrees Celsius.

    GO - Molecular functioni

    1. lysozyme activity Source: CACAO

    GO - Biological processi

    1. catabolism by organism of cell wall peptidoglycan in other organism Source: CACAO
    2. cytolysis Source: UniProtKB-KW
    3. defense response to bacterium Source: UniProtKB-KW
    4. viral release from host cell Source: UniProtKB-KW

    Keywords - Molecular functioni

    Antimicrobial, Bacteriolytic enzyme, Glycosidase, Hydrolase

    Keywords - Biological processi

    Cytolysis, Host cell lysis by virus, Virus exit from host cell

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lysozyme (EC:3.2.1.17)
    Alternative name(s):
    Lytic enzyme
    Muramidase
    Protein P15
    Gene namesi
    Name:XV
    OrganismiEnterobacteria phage PRD1 (Bacteriophage PRD1)
    Taxonomic identifieri10658 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageTectiviridaeTectivirus
    Virus hostiAcinetobacter calcoaceticus [TaxID: 471]
    Escherichia coli [TaxID: 562]
    Proteus mirabilis [TaxID: 584]
    Pseudomonas aeruginosa [TaxID: 287]
    Pseudomonas fluorescens [TaxID: 294]
    Pseudomonas putida (Arthrobacter siderocapsulatus) [TaxID: 303]
    Salmonella typhimurium [TaxID: 90371]
    Vibrio cholerae [TaxID: 666]
    ProteomesiUP000002143: Genome

    Subcellular locationi

    Virion membrane 1 Publication; Peripheral membrane protein 1 Publication
    Note: May be associated with the viral membrane via P20 and P22.

    GO - Cellular componenti

    1. virion membrane Source: CACAO

    Keywords - Cellular componenti

    Membrane, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 149149LysozymePRO_0000165348Add
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 24 family.Curated

    Family and domain databases

    InterProiIPR024408. DUF3380.
    IPR016284. Phage_PRD1_P15_lysozyme.
    [Graphical view]
    PfamiPF11860. DUF3380. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001069. Lytic_enz_p15. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P13559-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQYTLWDIIS RVESNGNLKA LRFEPEYYQR RMERGDWDNS IIQNIRAANK    50
    CSLGTARMIY CSSWGAVQIM GFNLYLNGAF NLSVAHFMEN EAYQVNEFRR 100
    FLLKNGLTEY TPERLASDKA ARVKFAKVYN GAESYADLIL QACQFYGVK 149
    Length:149
    Mass (Da):17,269
    Last modified:February 1, 1991 - v2
    Checksum:i50ABB718032939B5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14980 Genomic DNA. Translation: CAA33104.1.
    M22161 Genomic DNA. Translation: AAA32451.1.
    AY848689 Genomic DNA. Translation: AAX45908.1.
    PIRiS03568. LYBPD1.
    RefSeqiNP_040683.1. NC_001421.2.

    Genome annotation databases

    GeneIDi1260935.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14980 Genomic DNA. Translation: CAA33104.1 .
    M22161 Genomic DNA. Translation: AAA32451.1 .
    AY848689 Genomic DNA. Translation: AAX45908.1 .
    PIRi S03568. LYBPD1.
    RefSeqi NP_040683.1. NC_001421.2.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1260935.

    Family and domain databases

    InterProi IPR024408. DUF3380.
    IPR016284. Phage_PRD1_P15_lysozyme.
    [Graphical view ]
    Pfami PF11860. DUF3380. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001069. Lytic_enz_p15. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Comparison of the amino acid sequence of the lytic enzyme from broad-host-range bacteriophage PRD1 with sequences of other cell-wall-peptidoglycan lytic enzymes."
      Pakula T.M., Savilahti H., Bamford D.H.
      Eur. J. Biochem. 180:149-152(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The complete nucleotide sequence of the left very early region of Escherichia coli bacteriophage PRD1 coding for the terminal protein and the DNA polymerase."
      Savilahti H., Bamford D.H.
      Gene 57:121-130(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-98.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "A snapshot of viral evolution from genome analysis of the tectiviridae family."
      Saren A.M., Ravantti J.J., Benson S.D., Burnett R.M., Paulin L., Bamford D.H., Bamford J.K.H.
      J. Mol. Biol. 350:427-440(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    5. "Gene XV of bacteriophage PRD1 encodes a lytic enzyme with muramidase activity."
      Caldentey J., Hanninen A.L., Bamford D.H.
      Eur. J. Biochem. 225:341-346(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "The lytic enzyme of bacteriophage PRD1 is associated with the viral membrane."
      Rydman P.S., Bamford D.H.
      J. Bacteriol. 184:104-110(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiVLYS_BPPRD
    AccessioniPrimary (citable) accession number: P13559
    Secondary accession number(s): Q3T4P3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: February 1, 1991
    Last modified: October 1, 2014
    This is version 67 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3