P13543 (MLR_AEQIR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Myosin regulatory light chain, striated adductor muscle Short name=R-LC
Organism	Aequipecten irradians (Bay scallop) (Argopecten irradians)
Taxonomic identifier	31199 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Mollusca › Bivalvia › Pteriomorphia › Pectinoida › Pectinoidea › Pectinidae › Argopecten

Protein attributes

Sequence length	157 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	In molluscan muscle, calcium regulation is associated with myosin rather than with actin. Muscle myosin contains two types of light chains: the catalytic light chain, essential for ATPase activity, and the regulatory light chain, a calcium-binding protein responsible for Ca2+ dependent binding and Ca2+ dependent Mg-ATPase activity.
Miscellaneous	This chain binds calcium.
Sequence similarities	Contains 2 EF-hand domains.

Ontologies

Keywords
Domain	Repeat
Ligand	Calcium
Molecular function	Motor protein Muscle protein Myosin
Technical term	3D-structure
Gene Ontology (GO)
Cellular component	myosin complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed

Chain

2 – 157

156

Myosin regulatory light chain, striated adductor muscle

PRO_0000198751

Regions

Domain

16 – 51

36

EF-hand 1

Domain

85 – 120

36

EF-hand 2

Calcium binding

29 – 40

12

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

157

Helix Strand Turn

Details...

Helix

16 – 28

13

Helix

38 – 48

11

Helix

54 – 62

9

Beta strand

64 – 66

3

Helix

70 – 78

9

Helix

87 – 95

9

Helix

107 – 116

10

Beta strand

117 – 119

3

Helix

123 – 130

8

Helix

142 – 149

8

Sequences

	Sequence		Length	Mass (Da)
	P13543 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 243CE69DB02A7772	FASTA	157	17,671
	10 20 30 40 50 60 MADKAASGVL TKLPQKQIQE MKEAFSMIDV DRDGFVSKED IKAISEQLGR APDDKELTAM 70 80 90 100 110 120 LKEAPGPLNF TMFLSIFSDK LSGTDSEETI RNAFAMFDEQ ETKKLNIEYI KDLLENMGDN 130 140 150 FNKDEMRMTF KEAPVEGGKF DYVKFTAMIK GSGEEEA

References

[1]	"Cloning and characterization of the scallop essential and regulatory myosin light chain cDNAs." Goodwin E.B., Szent-Gyorgyi A.G., Leinwand L.A. J. Biol. Chem. 262:11052-11056(1987) [PubMed: 2440882] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Regulation of scallop myosin by mutant regulatory light chains." Goodwin E.B., Leinwand L.A., Szent-Gyorgyi A.G. J. Mol. Biol. 216:85-93(1990) [PubMed: 2146399] [Abstract] Cited for: SEQUENCE REVISION, MUTAGENESIS.
[3]	"Structure of the regulatory domain of scallop myosin at 2.8-A resolution." Xie X., Harrison D.H., Schlichting I., Sweet R.M., Kalabokis V.N., Szent-Gyorgyi A.G., Cohen C. Nature 368:306-312(1994) [PubMed: 8127365] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
[4]	"Structure of the regulatory domain of scallop myosin at 2-A resolution: implications for regulation." Houdusse A., Cohen C. Structure 4:21-32(1996) [PubMed: 8805510] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M17208 mRNA. Translation: AAA27715.1. Sequence problems.

PIR

B29786.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1B7T	X-ray	2.50	Y	2-157	[»]
1DFK	X-ray	4.20	Y	13-151	[»]
1DFL	X-ray	4.20	W/Y	13-151	[»]
1KK7	X-ray	3.20	Y	2-156	[»]
1KK8	X-ray	2.30	B	14-151	[»]
1KQM	X-ray	3.00	B	2-156	[»]
1KWO	X-ray	3.80	B	2-156	[»]
1L2O	X-ray	2.80	B	2-156	[»]
1QVI	X-ray	2.54	Y	2-156	[»]
1S5G	X-ray	3.10	Y	2-156	[»]
1SCM	X-ray	2.80	B	13-157	[»]
1SR6	X-ray	2.75	B	2-156	[»]
1WDC	X-ray	2.00	B	2-157	[»]
2W4T	electron microscopy	35.00	Y	16-151	[»]
2W4V	electron microscopy	35.00	Y	16-151	[»]
2W4W	electron microscopy	35.00	Y	16-151	[»]
3JTD	X-ray	2.57	B	2-157	[»]
3JVT	X-ray	2.10	B	2-157	[»]

ProteinModelPortal

P13543.

SMR

P13543. Positions 12-155.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

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Family and domain databases

InterPro

IPR011992. EF-hand-like_dom.
IPR018247. EF_Hand_1_Ca_BS.
IPR018249. EF_HAND_2.
IPR002048. EF_hand_Ca-bd.
[Graphical view]

Gene3D

G3DSA:1.10.238.10. EF-Hand_type. 2 hits.

SMART

SM00054. EFh. 2 hits.
[Graphical view]

PROSITE

PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 2 hits.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

MLR_AEQIR

Accession

Primary (citable) accession number: P13543

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 23, 2007
Last modified:	July 27, 2011
This is version 83 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)