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P13542 (MYH8_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Myosin-8
Alternative name(s):
Myosin heavy chain 8
Myosin heavy chain, skeletal muscle, perinatal
Short name=MyHC-perinatal
Gene names
Name:Myh8
Synonyms:Myhsp
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length1937 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Muscle contraction.

Subunit structure

Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).

Subcellular location

Cytoplasm › myofibril. Note: Thick filaments of the myofibrils.

Domain

The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.

Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).

Sequence similarities

Contains 1 IQ domain.

Contains 1 myosin head-like domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 19371937Myosin-8
PRO_0000123414

Regions

Domain1 – 780780Myosin head-like
Domain781 – 81333IQ
Nucleotide binding181 – 1888ATP
Region658 – 68023Actin-binding
Region760 – 77415Actin-binding
Coiled coil842 – 19371096 Potential

Amino acid modifications

Modified residue1321N6,N6,N6-trimethyllysine Potential
Modified residue9511Phosphoserine Ref.3
Modified residue12021Phosphoserine Ref.3

Sequences

Sequence LengthMass (Da)Tools
P13542 [UniParc].

Last modified August 30, 2005. Version 2.
Checksum: 5C215BC2757B64A3

FASTA1,937222,708
        10         20         30         40         50         60 
MSAGSDAEMA IFGEAAPYLR KSEKERIEAQ NKPFDAKTSV FVAEPKESYV KSVIQSKDGG 

        70         80         90        100        110        120 
KVTVKTESGA TLTVKEDQVF PMNPPKYDKI EDMAMMTHLH EPGVLYNLKE RYAAWMIYTY 

       130        140        150        160        170        180 
SGLFCVTVNP YKWLPVYNPE VVAAYRGKKR QEAPPHIFSI SDNAYQFMLT DRENQSILIT 

       190        200        210        220        230        240 
GESGAGKTVN TKRVIQYFAT IAVTGDKKKE ESGKMQGTLE DQIISANPLL EAFGNAKTVR 

       250        260        270        280        290        300 
NDNSSRFGKF IRIHFGTTGK LASADIETYL LEKSRVTFQL KAERSYHIFY QITSNKKPEL 

       310        320        330        340        350        360 
IEMLLITTNP YDYAFVSQGE ITVPSIDDQE ELMATDSAID ILGFSPEEKV SIYKLTGAVM 

       370        380        390        400        410        420 
HYGNMKFKQK QREEQAEPDG TEVADKAAYL QCLNSADLLK ALCYPRVKVG NEYVTKGQTV 

       430        440        450        460        470        480 
QQVYNAVGAL AKAVYEKMFL WMVTRINQQL DTKQPRQYFI GVLDIAGFEI FDFNSLEQLC 

       490        500        510        520        530        540 
INFTNEKLQQ FFNHHMFVLE QEEYKKEGIE WTFIDFGMDL AACIELIEKP LGIFSILEEE 

       550        560        570        580        590        600 
CMFPKATDTS FKNKLYDQHL GKSNNFQKPK PTKGKAEAHF SLVHYAGTVD YNITGWLDKN 

       610        620        630        640        650        660 
KDPLNDTVVG LYQKSAMKTL ASLFSTYASA EADGGAKKGA KKKGSSFQTV SALFRENLNK 

       670        680        690        700        710        720 
LMTNLRSTHP HFVRCIIPNE TKTPGAMEHE LVLHQLRCNG VLEGIRICRK GFPSRILYGD 

       730        740        750        760        770        780 
FKQRYKVLNA SAIPEGQFID SKKASEKLLG SIDIDHTQYK FGHTKVFFKA GLLGLLEEMR 

       790        800        810        820        830        840 
DEKLAQIITR TQAVCRGYLM RVEYQKMLLR RESIFCIQYN VRAFMNVKHW PWMKLFFKIK 

       850        860        870        880        890        900 
PLLKSAETEK EMATMKEEFQ KTKDELAKSE AKRKELEEKM VTLLKEKNDL QLQVQSEADS 

       910        920        930        940        950        960 
LADAEERCEQ LIKNKIQLEA KIKEVTERAE DEEEINAELT AKKRKLEDEC SELKKDIDDL 

       970        980        990       1000       1010       1020 
ELTLAKVEKE KHATENKVKN LTEEMAGLDE NIAKLTKEKK ALQEAHQQTL DDLQAEEDKV 

      1030       1040       1050       1060       1070       1080 
NTLTKAKTKL EQQVDDLEGS LEQEKKLRMD LERAKRKLEG DLKLAQESTM DIENDKQQLD 

      1090       1100       1110       1120       1130       1140 
EKLKKKEFEI SNLISKIEDE QAVEIQLQKK IKELQARIEE LEEEIEAERA SRAKAEKQRS 

      1150       1160       1170       1180       1190       1200 
DLSRELEEIS ERLEEAGGAT SAQVEMNKKR ETEFQKLRRD LEEATLQHEA TAAALRKKHA 

      1210       1220       1230       1240       1250       1260 
DSVAELGEQI DNLQRVKQKL EKEKSELKME IDDLSSNAEA IAKAKGNLEK MCRTLEDQVS 

      1270       1280       1290       1300       1310       1320 
ELKSKEEEQQ RLINELTAQR ARLQTEAGEY SRQLDEKDAL VSQLSRSKQA STQQIEELKR 

      1330       1340       1350       1360       1370       1380 
QLEEETKAKN ALAHALQSSR HDCDLLREQY EEEQEGKAEL QRALSKANSE VAQWRTKYET 

      1390       1400       1410       1420       1430       1440 
DAIQRTEELE EAKKKLAQRL QAAEEHVEAV NAKCASLEKT KQRLQNEVED LMIDVERTNA 

      1450       1460       1470       1480       1490       1500 
ACAALDKKQR NFDKVLSEWR QKYEETQAEL ESCQKESRTL STELFKVKNA YEESLDHLET 

      1510       1520       1530       1540       1550       1560 
LRRENKNLQQ EISDLTEQIA EGGKHIHELE KIKKQVEQEK CEIQAALEEA EASLEHEEGK 

      1570       1580       1590       1600       1610       1620 
ILRIQLELNQ VKSEIDRKIA EKDEEIDQLK RNHVRVVETM QSTLDAEIRS RNDALRVKKK 

      1630       1640       1650       1660       1670       1680 
MEGDLNEMEI QLNHANRLAA ESLRNYRNTQ GILKDTQLHL DDALRGQEDL KEQLAIVERR 

      1690       1700       1710       1720       1730       1740 
ANLLQAEIEE LRATLEQTER SRKIAEQELL DASERVQLLH TQNTSLINTK KKLENDVSQL 

      1750       1760       1770       1780       1790       1800 
QSEVEEVIQE SRNAEEKAKK AITDAAMMAE ELKKEQDTSA HLERMKKNME QTVKDLQHRL 

      1810       1820       1830       1840       1850       1860 
DEAEQLALKG GKKQIQKLEA RVRELEGEVE NEQKRNAEAV KGLRKHERRV KELTYQTEED 

      1870       1880       1890       1900       1910       1920 
RKNVLRLQDL VDKLQAKVKS YKRQAEEAEE QSNANLAKFR KLQHELEEAE ERADIAESQV 

      1930 
NKLRVKSREV HTKISAE

« Hide

References

« Hide 'large scale' references
[1]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[2]"Genes for skeletal muscle myosin heavy chains are clustered and are not located on the same mouse chromosome as a cardiac myosin heavy chain gene."
Weydert A., Daubas P., Lazaridis I., Barton P., Garner I., Leader D.P., Bonhomme F., Catalan J., Simon D., Guenet J.-L., Gros F., Buckingham M.E.
Proc. Natl. Acad. Sci. U.S.A. 82:7183-7187(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1824-1937.
[3]"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry."
Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M.
J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-951 AND SER-1202, MASS SPECTROMETRY.
Tissue: Melanoma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AL596129 Genomic DNA. Translation: CAI24991.1.
M12289 mRNA. Translation: AAA39795.1.
IPIIPI00265380.
PIRB24733.
RefSeqNP_796343.2. NM_177369.3.
UniGeneMm.340163.

3D structure databases

ProteinModelPortalP13542.
SMRP13542. Positions 6-966.
ModBaseSearch...

PTM databases

PhosphoSiteP13542.

Proteomic databases

PRIDEP13542.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000019625; ENSMUSP00000019625; ENSMUSG00000055775.
GeneID17885.
KEGGmmu:17885.
UCSCuc007jmn.1. mouse.

Organism-specific databases

CTD4626.
MGIMGI:1339712. Myh8.

Phylogenomic databases

GeneTreeENSGT00680000099790.
HOGENOMHOG000173959.
HOVERGENHBG004704.
InParanoidP13542.
KOK10352.
OMAQLKRNHT.
OrthoDBEOG43N7BR.

Gene expression databases

ArrayExpressP13542.
BgeeP13542.
GenevestigatorP13542.

Family and domain databases

Gene3D4.10.270.10. 1 hit.
InterProIPR000048. IQ_motif_EF-hand-BS.
IPR027401. Myosin-like_IQ_dom.
IPR015650. Myosin_1/23/4/7/8/13/15.
IPR001609. Myosin_head_motor_dom.
IPR004009. Myosin_N.
IPR002928. Myosin_tail.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERPTHR13140:SF22. PTHR13140:SF22. 1 hit.
PfamPF00063. Myosin_head. 1 hit.
PF02736. Myosin_N. 1 hit.
PF01576. Myosin_tail_1. 1 hit.
[Graphical view]
PRINTSPR00193. MYOSINHEAVY.
SMARTSM00015. IQ. 1 hit.
SM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS50096. IQ. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio292697.
SOURCESearch...

Entry information

Entry nameMYH8_MOUSE
AccessionPrimary (citable) accession number: P13542
Secondary accession number(s): Q5SX36
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: August 30, 2005
Last modified: May 29, 2013
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents