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P13542

- MYH8_MOUSE

UniProt

P13542 - MYH8_MOUSE

Protein

Myosin-8

Gene

Myh8

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 2 (30 Aug 2005)
      Previous versions | rss
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    Functioni

    Muscle contraction.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi181 – 1888ATP

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. motor activity Source: InterPro
    3. protein binding Source: MGI

    Keywords - Molecular functioni

    Motor protein, Muscle protein, Myosin

    Keywords - Ligandi

    Actin-binding, ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Myosin-8
    Alternative name(s):
    Myosin heavy chain 8
    Myosin heavy chain, skeletal muscle, perinatal
    Short name:
    MyHC-perinatal
    Gene namesi
    Name:Myh8
    Synonyms:Myhsp
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:1339712. Myh8.

    Subcellular locationi

    Cytoplasmmyofibril
    Note: Thick filaments of the myofibrils.

    GO - Cellular componenti

    1. focal adhesion Source: Ensembl
    2. myofibril Source: UniProtKB-SubCell
    3. myosin complex Source: MGI
    4. myosin filament Source: UniProtKB-KW
    5. nucleolus Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm, Thick filament

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 19371937Myosin-8PRO_0000123414Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei132 – 1321N6,N6,N6-trimethyllysineSequence Analysis

    Keywords - PTMi

    Methylation

    Proteomic databases

    MaxQBiP13542.
    PRIDEiP13542.

    PTM databases

    PhosphoSiteiP13542.

    Expressioni

    Gene expression databases

    BgeeiP13542.
    GenevestigatoriP13542.

    Interactioni

    Subunit structurei

    Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).

    Protein-protein interaction databases

    BioGridi201649. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP13542.
    SMRiP13542. Positions 6-966.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini88 – 781694Myosin motorAdd
    BLAST
    Domaini781 – 81333IQPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni658 – 68023Actin-bindingAdd
    BLAST
    Regioni760 – 77415Actin-bindingAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili842 – 19371096Sequence AnalysisAdd
    BLAST

    Domaini

    The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).

    Sequence similaritiesi

    Contains 1 IQ domain.PROSITE-ProRule annotation
    Contains 1 myosin motor domain.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    GeneTreeiENSGT00720000108596.
    HOGENOMiHOG000173959.
    HOVERGENiHBG004704.
    InParanoidiP13542.
    KOiK10352.
    OMAiQLKRNHT.
    OrthoDBiEOG7RBZ7G.
    PhylomeDBiP13542.
    TreeFamiTF314375.

    Family and domain databases

    Gene3Di4.10.270.10. 1 hit.
    InterProiIPR000048. IQ_motif_EF-hand-BS.
    IPR027401. Myosin-like_IQ_dom.
    IPR001609. Myosin_head_motor_dom.
    IPR004009. Myosin_N.
    IPR002928. Myosin_tail.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00063. Myosin_head. 1 hit.
    PF02736. Myosin_N. 1 hit.
    PF01576. Myosin_tail_1. 1 hit.
    [Graphical view]
    PRINTSiPR00193. MYOSINHEAVY.
    SMARTiSM00015. IQ. 1 hit.
    SM00242. MYSc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS50096. IQ. 1 hit.
    PS51456. MYOSIN_MOTOR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P13542-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSAGSDAEMA IFGEAAPYLR KSEKERIEAQ NKPFDAKTSV FVAEPKESYV     50
    KSVIQSKDGG KVTVKTESGA TLTVKEDQVF PMNPPKYDKI EDMAMMTHLH 100
    EPGVLYNLKE RYAAWMIYTY SGLFCVTVNP YKWLPVYNPE VVAAYRGKKR 150
    QEAPPHIFSI SDNAYQFMLT DRENQSILIT GESGAGKTVN TKRVIQYFAT 200
    IAVTGDKKKE ESGKMQGTLE DQIISANPLL EAFGNAKTVR NDNSSRFGKF 250
    IRIHFGTTGK LASADIETYL LEKSRVTFQL KAERSYHIFY QITSNKKPEL 300
    IEMLLITTNP YDYAFVSQGE ITVPSIDDQE ELMATDSAID ILGFSPEEKV 350
    SIYKLTGAVM HYGNMKFKQK QREEQAEPDG TEVADKAAYL QCLNSADLLK 400
    ALCYPRVKVG NEYVTKGQTV QQVYNAVGAL AKAVYEKMFL WMVTRINQQL 450
    DTKQPRQYFI GVLDIAGFEI FDFNSLEQLC INFTNEKLQQ FFNHHMFVLE 500
    QEEYKKEGIE WTFIDFGMDL AACIELIEKP LGIFSILEEE CMFPKATDTS 550
    FKNKLYDQHL GKSNNFQKPK PTKGKAEAHF SLVHYAGTVD YNITGWLDKN 600
    KDPLNDTVVG LYQKSAMKTL ASLFSTYASA EADGGAKKGA KKKGSSFQTV 650
    SALFRENLNK LMTNLRSTHP HFVRCIIPNE TKTPGAMEHE LVLHQLRCNG 700
    VLEGIRICRK GFPSRILYGD FKQRYKVLNA SAIPEGQFID SKKASEKLLG 750
    SIDIDHTQYK FGHTKVFFKA GLLGLLEEMR DEKLAQIITR TQAVCRGYLM 800
    RVEYQKMLLR RESIFCIQYN VRAFMNVKHW PWMKLFFKIK PLLKSAETEK 850
    EMATMKEEFQ KTKDELAKSE AKRKELEEKM VTLLKEKNDL QLQVQSEADS 900
    LADAEERCEQ LIKNKIQLEA KIKEVTERAE DEEEINAELT AKKRKLEDEC 950
    SELKKDIDDL ELTLAKVEKE KHATENKVKN LTEEMAGLDE NIAKLTKEKK 1000
    ALQEAHQQTL DDLQAEEDKV NTLTKAKTKL EQQVDDLEGS LEQEKKLRMD 1050
    LERAKRKLEG DLKLAQESTM DIENDKQQLD EKLKKKEFEI SNLISKIEDE 1100
    QAVEIQLQKK IKELQARIEE LEEEIEAERA SRAKAEKQRS DLSRELEEIS 1150
    ERLEEAGGAT SAQVEMNKKR ETEFQKLRRD LEEATLQHEA TAAALRKKHA 1200
    DSVAELGEQI DNLQRVKQKL EKEKSELKME IDDLSSNAEA IAKAKGNLEK 1250
    MCRTLEDQVS ELKSKEEEQQ RLINELTAQR ARLQTEAGEY SRQLDEKDAL 1300
    VSQLSRSKQA STQQIEELKR QLEEETKAKN ALAHALQSSR HDCDLLREQY 1350
    EEEQEGKAEL QRALSKANSE VAQWRTKYET DAIQRTEELE EAKKKLAQRL 1400
    QAAEEHVEAV NAKCASLEKT KQRLQNEVED LMIDVERTNA ACAALDKKQR 1450
    NFDKVLSEWR QKYEETQAEL ESCQKESRTL STELFKVKNA YEESLDHLET 1500
    LRRENKNLQQ EISDLTEQIA EGGKHIHELE KIKKQVEQEK CEIQAALEEA 1550
    EASLEHEEGK ILRIQLELNQ VKSEIDRKIA EKDEEIDQLK RNHVRVVETM 1600
    QSTLDAEIRS RNDALRVKKK MEGDLNEMEI QLNHANRLAA ESLRNYRNTQ 1650
    GILKDTQLHL DDALRGQEDL KEQLAIVERR ANLLQAEIEE LRATLEQTER 1700
    SRKIAEQELL DASERVQLLH TQNTSLINTK KKLENDVSQL QSEVEEVIQE 1750
    SRNAEEKAKK AITDAAMMAE ELKKEQDTSA HLERMKKNME QTVKDLQHRL 1800
    DEAEQLALKG GKKQIQKLEA RVRELEGEVE NEQKRNAEAV KGLRKHERRV 1850
    KELTYQTEED RKNVLRLQDL VDKLQAKVKS YKRQAEEAEE QSNANLAKFR 1900
    KLQHELEEAE ERADIAESQV NKLRVKSREV HTKISAE 1937
    Length:1,937
    Mass (Da):222,708
    Last modified:August 30, 2005 - v2
    Checksum:i5C215BC2757B64A3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL596129 Genomic DNA. Translation: CAI24991.1.
    M12289 mRNA. Translation: AAA39795.1.
    CCDSiCCDS24857.1.
    PIRiB24733.
    RefSeqiNP_796343.2. NM_177369.3.
    UniGeneiMm.340163.

    Genome annotation databases

    EnsembliENSMUST00000019625; ENSMUSP00000019625; ENSMUSG00000055775.
    GeneIDi17885.
    KEGGimmu:17885.
    UCSCiuc007jmn.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL596129 Genomic DNA. Translation: CAI24991.1 .
    M12289 mRNA. Translation: AAA39795.1 .
    CCDSi CCDS24857.1.
    PIRi B24733.
    RefSeqi NP_796343.2. NM_177369.3.
    UniGenei Mm.340163.

    3D structure databases

    ProteinModelPortali P13542.
    SMRi P13542. Positions 6-966.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201649. 1 interaction.

    PTM databases

    PhosphoSitei P13542.

    Proteomic databases

    MaxQBi P13542.
    PRIDEi P13542.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000019625 ; ENSMUSP00000019625 ; ENSMUSG00000055775 .
    GeneIDi 17885.
    KEGGi mmu:17885.
    UCSCi uc007jmn.1. mouse.

    Organism-specific databases

    CTDi 4626.
    MGIi MGI:1339712. Myh8.

    Phylogenomic databases

    GeneTreei ENSGT00720000108596.
    HOGENOMi HOG000173959.
    HOVERGENi HBG004704.
    InParanoidi P13542.
    KOi K10352.
    OMAi QLKRNHT.
    OrthoDBi EOG7RBZ7G.
    PhylomeDBi P13542.
    TreeFami TF314375.

    Miscellaneous databases

    NextBioi 292697.
    PROi P13542.
    SOURCEi Search...

    Gene expression databases

    Bgeei P13542.
    Genevestigatori P13542.

    Family and domain databases

    Gene3Di 4.10.270.10. 1 hit.
    InterProi IPR000048. IQ_motif_EF-hand-BS.
    IPR027401. Myosin-like_IQ_dom.
    IPR001609. Myosin_head_motor_dom.
    IPR004009. Myosin_N.
    IPR002928. Myosin_tail.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00063. Myosin_head. 1 hit.
    PF02736. Myosin_N. 1 hit.
    PF01576. Myosin_tail_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00193. MYOSINHEAVY.
    SMARTi SM00015. IQ. 1 hit.
    SM00242. MYSc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS50096. IQ. 1 hit.
    PS51456. MYOSIN_MOTOR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    2. "Genes for skeletal muscle myosin heavy chains are clustered and are not located on the same mouse chromosome as a cardiac myosin heavy chain gene."
      Weydert A., Daubas P., Lazaridis I., Barton P., Garner I., Leader D.P., Bonhomme F., Catalan J., Simon D., Guenet J.-L., Gros F., Buckingham M.E.
      Proc. Natl. Acad. Sci. U.S.A. 82:7183-7187(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1824-1937.

    Entry informationi

    Entry nameiMYH8_MOUSE
    AccessioniPrimary (citable) accession number: P13542
    Secondary accession number(s): Q5SX36
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: August 30, 2005
    Last modified: October 1, 2014
    This is version 110 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3