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P13541 (MYH3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Myosin-3
Alternative name(s):
Myosin heavy chain 3
Gene names
Name:Myh3
Synonyms:Myhse
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1940 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Muscle contraction.

Subunit structure

Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).

Subcellular location

Cytoplasmmyofibril. Note: Thick filaments of the myofibrils.

Domain

The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.

Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).

Sequence similarities

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.

Contains 1 IQ domain.

Contains 1 myosin motor domain.

Caution

Represents a conventional myosin. This protein should not be confused with the unconventional myosin-3 (MYO3).

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 19401940Myosin-3
PRO_0000123395

Regions

Domain86 – 779694Myosin motor
Domain782 – 81130IQ
Nucleotide binding179 – 1868ATP Potential
Region656 – 67823Actin-binding By similarity
Region758 – 77215Actin-binding By similarity
Coiled coil841 – 19281088 Potential

Amino acid modifications

Modified residue1301N6,N6,N6-trimethyllysine Potential

Experimental info

Sequence conflict17471R → Q in AAA39791. Ref.3
Sequence conflict18581D → A in AAA39791. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P13541 [UniParc].

Last modified January 9, 2007. Version 2.
Checksum: 9CF748702D8353C9

FASTA1,940223,791
        10         20         30         40         50         60 
MSSDTEMEVF GIAAPFLRKS EKERIEAQNQ PFDAKTYCFV VDSKEEYVKG KIKSSQDGKV 

        70         80         90        100        110        120 
TVETEDSRTL VVKPEDVYAM NPPKFDKIED MAMLTHLNEP AVLYNLKDRY TSWMIYTYSG 

       130        140        150        160        170        180 
LFCVTVNPYK WLPVYNPEVV DGYRGKKRQE APPHIFSISD NAYQFMLTDR ENQSILITGE 

       190        200        210        220        230        240 
SGAGKTVNTK RVIQYFATIA ATGDLAKKKD SKMKGTLEDQ IISANPLLEA FGNAKTVRND 

       250        260        270        280        290        300 
NSSRFGKFIR IHFGTTGKLA SADIETYLLE KSRVTFQLKA ERSYHIFYQI LSNKKPELIE 

       310        320        330        340        350        360 
LLLITTNPYD YPFISQGEIL VASIDDAEEL LATDSAIDIL GFTPEEKSGL YKLTGAVMHY 

       370        380        390        400        410        420 
GNMKFKQKQR EEQAEPDGTE VADKTAYLMG LNSSDLLKAL CFPRVKVGNE YVTKGQTVDQ 

       430        440        450        460        470        480 
VHHAVNALSK SVYEKLFLWM VTRINQQLDT KLPRQHFIGV LDIAGFEIFE YNSLEQLCIN 

       490        500        510        520        530        540 
FTNEKLQQFF NHHMFVLEQE EYKKEGIEWT FIDFGMDLAA CIELIEKPMG IFSILEEECM 

       550        560        570        580        590        600 
FPKATDTSFK NKLYDQHLGK SNNFQKPKVV KGKAEAHFSL VHYAGTVDYS VSGWLEKNKD 

       610        620        630        640        650        660 
PLNETVVGLY QKSSNRLLAH LYATFATTDA DGGKKKVAKK KGSSFQTVSA LFRENLNKLM 

       670        680        690        700        710        720 
SNLRTTHPHF VRCIIPNETK TPGAMEHSLV LHQLRCNGVL EGIRICRKGF PNRILYGDFK 

       730        740        750        760        770        780 
QRYRVLNASA IPEGQFIDSK KACEKLLASI DIDHTQYKFG HTKVFFKAGL LGTLEEMRDE 

       790        800        810        820        830        840 
RLAKLITRTQ AVCRGFLMRV EFQKMMQRRE SIFCIQYNIR AFMNVKHWPW MKLFFKIKPL 

       850        860        870        880        890        900 
LKSAETEKEM ATMKEEFQKT KDELAKSEAK RKELEEKLVT LVQEKNDLQL QVQAESENLL 

       910        920        930        940        950        960 
DAEERCDQLI KAKFQLEAKI KEVTERAEDE EEINAELTAK KRKLEDECSE LKKDIDDLEL 

       970        980        990       1000       1010       1020 
TLAKVEKEKH ATENKVKNLT EELAGLDETI AKLTREKKAL QEAHQQTLDD LQAEEDKVNS 

      1030       1040       1050       1060       1070       1080 
LSKLKSKLEQ QVDDLESSLE QEKKLRVDLE RNKRKLEGDL KLAQESILDL ENDKQQLDER 

      1090       1100       1110       1120       1130       1140 
LKKKDFEYSQ LQSKVEDEQT LSLQLQKKIK ELQARIEELE EEIEAERATR AKTEKQRSDY 

      1150       1160       1170       1180       1190       1200 
ARELEELSER LEEAGGVTST QIELNKKREA EFLKLRRDLE EATLQHEATV ATLRKKHADS 

      1210       1220       1230       1240       1250       1260 
AAELAEQIDN LQRVKQKLEK EKSEFKLEID DLSSSVESVS KSKANLEKIC RTLEDQLSEA 

      1270       1280       1290       1300       1310       1320 
RGKNEEMQRS LSELTTQKSR LQTEAGELSR QLEEKESIVS QLSRSKQAFT QQIEELKRQL 

      1330       1340       1350       1360       1370       1380 
EEENKAKNAL AHALQSSRHD CDLLREQYEE EQEGKAELQR ALSKANSEVA QWRTKYETDA 

      1390       1400       1410       1420       1430       1440 
IQRTEELEEA KKKLAQRLQD SEEQVEAVNA KCASLEKTKQ RLQGEVEDLM VDVERANSLA 

      1450       1460       1470       1480       1490       1500 
AALDKKQRNF DKVLAEWKTK CEESQAELEA ALKESRSLST ELFKLKNAYE EALDQLETVK 

      1510       1520       1530       1540       1550       1560 
RENKNLEQEI ADLTEQIAEN GKSIHELEKS RKQMELEKAD IQMALEEAEA ALEHEEAKIL 

      1570       1580       1590       1600       1610       1620 
RIQLELTQVK SEIDRKIAEK DEEIEQLKRN YQRTVETMQG ALDAEVRSRN EAIRLKKKME 

      1630       1640       1650       1660       1670       1680 
GDLNEIEIQL SHANRQAAET IKHLRSVQGQ LKDTQLHLDD ALRGQEDLKE QLAIVERRAN 

      1690       1700       1710       1720       1730       1740 
LLQAEVEELR ATLEQTERAR KLAEQELLDS NERVQLLHTQ NTSLIHTKKK LETDLTQLQS 

      1750       1760       1770       1780       1790       1800 
EVEDACRDAR NAEEKAKKAI TDAAMMAEEL KKEQDTSAHL ERMKKNLEQT VKDLQHRLDE 

      1810       1820       1830       1840       1850       1860 
AEQLALKGGK KQIQKLETRI RELEFELEGE QKRNTESVKG LRKYERRVKE LTYQSEEDRK 

      1870       1880       1890       1900       1910       1920 
NVLRLQDLVD KLQVKVKSYK RQAEEADEQA NAHLTKFRKA QHELEEAEER ADIAESQVNK 

      1930       1940 
LRAKTRDFTS SRMVVHESEE 

« Hide

References

« Hide 'large scale' references
[1]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 523-1292.
Strain: C57BL/6J.
Tissue: Head.
[3]"Genes for skeletal muscle myosin heavy chains are clustered and are not located on the same mouse chromosome as a cardiac myosin heavy chain gene."
Weydert A., Daubas P., Lazaridis I., Barton P., Garner I., Leader D.P., Bonhomme F., Catalan J., Simon D., Guenet J.-L., Gros F., Buckingham M.E.
Proc. Natl. Acad. Sci. U.S.A. 82:7183-7187(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1747-1905.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL645988, AL596129 Genomic DNA. Translation: CAI25982.2.
AL596129, AL645988 Genomic DNA. Translation: CAI24992.2.
AK132623 mRNA. Translation: BAE21268.1.
M11154 mRNA. Translation: AAA39791.1.
CCDSCCDS36184.1.
PIRA24733.
RefSeqNP_001093105.1. NM_001099635.1.
XP_006532475.1. XM_006532412.1.
UniGeneMm.340090.

3D structure databases

ProteinModelPortalP13541.
SMRP13541. Positions 4-964.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid201647. 5 interactions.

PTM databases

PhosphoSiteP13541.

Proteomic databases

MaxQBP13541.
PaxDbP13541.
PRIDEP13541.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000007301; ENSMUSP00000007301; ENSMUSG00000020908.
ENSMUST00000108689; ENSMUSP00000104329; ENSMUSG00000020908.
ENSMUST00000165221; ENSMUSP00000131883; ENSMUSG00000020908.
GeneID17883.
KEGGmmu:17883.
UCSCuc007jlx.1. mouse.

Organism-specific databases

CTD4621.
MGIMGI:1339709. Myh3.

Phylogenomic databases

eggNOGCOG5022.
GeneTreeENSGT00720000108596.
HOGENOMHOG000173959.
HOVERGENHBG004704.
InParanoidP13541.
KOK10352.
OMADEQANAH.
OrthoDBEOG7RBZ7G.
PhylomeDBP13541.
TreeFamTF314375.

Gene expression databases

BgeeP13541.
GenevestigatorP13541.

Family and domain databases

Gene3D4.10.270.10. 1 hit.
InterProIPR000048. IQ_motif_EF-hand-BS.
IPR027401. Myosin-like_IQ_dom.
IPR001609. Myosin_head_motor_dom.
IPR004009. Myosin_N.
IPR002928. Myosin_tail.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00063. Myosin_head. 1 hit.
PF02736. Myosin_N. 1 hit.
PF01576. Myosin_tail_1. 1 hit.
[Graphical view]
PRINTSPR00193. MYOSINHEAVY.
SMARTSM00015. IQ. 2 hits.
SM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS50096. IQ. 1 hit.
PS51456. MYOSIN_MOTOR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio292685.
PROP13541.
SOURCESearch...

Entry information

Entry nameMYH3_MOUSE
AccessionPrimary (citable) accession number: P13541
Secondary accession number(s): Q3V183, Q5SUD4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 9, 2007
Last modified: July 9, 2014
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot