P13541 (MYH3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Myosin-3

Alternative name(s):
Myosin heavy chain 3

Gene names

Name:	Myh3
Synonyms:	Myhse

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1940 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Muscle contraction.
Subunit structure	Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).
Subcellular location	Cytoplasm › myofibril. Note: Thick filaments of the myofibrils.
Domain	The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils. Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
Sequence similarities	Contains 1 IQ domain. Contains 1 myosin head-like domain.
Caution	Represents a conventional myosin. This protein should not be confused with the unconventional myosin-3 (MYO3).

Ontologies

Keywords
Cellular component	Cytoplasm Thick filament
Domain	Coiled coil
Ligand	ATP-binding Actin-binding Calmodulin-binding Nucleotide-binding
Molecular function	Motor protein Muscle protein Myosin
PTM	Methylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	contractile fiber Inferred from direct assay PubMed 16810681. Source: MGI myofibril Inferred from electronic annotation. Source: UniProtKB-SubCell myosin complex Inferred from direct assay PubMed 1728586. Source: MGI myosin filament Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW motor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1940	1940	Myosin-3		PRO_0000123395
Regions
	Domain	1 – 781	781	Myosin head-like
	Domain	782 – 811	30	IQ
	Nucleotide binding	179 – 186	8	ATP Potential
	Region	656 – 678	23	Actin-binding By similarity
	Region	758 – 772	15	Actin-binding By similarity
	Coiled coil	841 – 1928	1088	Potential
Amino acid modifications
	Modified residue	130	1	N6,N6,N6-trimethyllysine Potential
Experimental info
	Sequence conflict	1747	1	R → Q in AAA39791. Ref.3
	Sequence conflict	1858	1	D → A in AAA39791. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P13541 [UniParc]. Last modified January 9, 2007. Version 2. Checksum: 9CF748702D8353C9	FASTA	1,940	223,791
	10 20 30 40 50 60 MSSDTEMEVF GIAAPFLRKS EKERIEAQNQ PFDAKTYCFV VDSKEEYVKG KIKSSQDGKV 70 80 90 100 110 120 TVETEDSRTL VVKPEDVYAM NPPKFDKIED MAMLTHLNEP AVLYNLKDRY TSWMIYTYSG 130 140 150 160 170 180 LFCVTVNPYK WLPVYNPEVV DGYRGKKRQE APPHIFSISD NAYQFMLTDR ENQSILITGE 190 200 210 220 230 240 SGAGKTVNTK RVIQYFATIA ATGDLAKKKD SKMKGTLEDQ IISANPLLEA FGNAKTVRND 250 260 270 280 290 300 NSSRFGKFIR IHFGTTGKLA SADIETYLLE KSRVTFQLKA ERSYHIFYQI LSNKKPELIE 310 320 330 340 350 360 LLLITTNPYD YPFISQGEIL VASIDDAEEL LATDSAIDIL GFTPEEKSGL YKLTGAVMHY 370 380 390 400 410 420 GNMKFKQKQR EEQAEPDGTE VADKTAYLMG LNSSDLLKAL CFPRVKVGNE YVTKGQTVDQ 430 440 450 460 470 480 VHHAVNALSK SVYEKLFLWM VTRINQQLDT KLPRQHFIGV LDIAGFEIFE YNSLEQLCIN 490 500 510 520 530 540 FTNEKLQQFF NHHMFVLEQE EYKKEGIEWT FIDFGMDLAA CIELIEKPMG IFSILEEECM 550 560 570 580 590 600 FPKATDTSFK NKLYDQHLGK SNNFQKPKVV KGKAEAHFSL VHYAGTVDYS VSGWLEKNKD 610 620 630 640 650 660 PLNETVVGLY QKSSNRLLAH LYATFATTDA DGGKKKVAKK KGSSFQTVSA LFRENLNKLM 670 680 690 700 710 720 SNLRTTHPHF VRCIIPNETK TPGAMEHSLV LHQLRCNGVL EGIRICRKGF PNRILYGDFK 730 740 750 760 770 780 QRYRVLNASA IPEGQFIDSK KACEKLLASI DIDHTQYKFG HTKVFFKAGL LGTLEEMRDE 790 800 810 820 830 840 RLAKLITRTQ AVCRGFLMRV EFQKMMQRRE SIFCIQYNIR AFMNVKHWPW MKLFFKIKPL 850 860 870 880 890 900 LKSAETEKEM ATMKEEFQKT KDELAKSEAK RKELEEKLVT LVQEKNDLQL QVQAESENLL 910 920 930 940 950 960 DAEERCDQLI KAKFQLEAKI KEVTERAEDE EEINAELTAK KRKLEDECSE LKKDIDDLEL 970 980 990 1000 1010 1020 TLAKVEKEKH ATENKVKNLT EELAGLDETI AKLTREKKAL QEAHQQTLDD LQAEEDKVNS 1030 1040 1050 1060 1070 1080 LSKLKSKLEQ QVDDLESSLE QEKKLRVDLE RNKRKLEGDL KLAQESILDL ENDKQQLDER 1090 1100 1110 1120 1130 1140 LKKKDFEYSQ LQSKVEDEQT LSLQLQKKIK ELQARIEELE EEIEAERATR AKTEKQRSDY 1150 1160 1170 1180 1190 1200 ARELEELSER LEEAGGVTST QIELNKKREA EFLKLRRDLE EATLQHEATV ATLRKKHADS 1210 1220 1230 1240 1250 1260 AAELAEQIDN LQRVKQKLEK EKSEFKLEID DLSSSVESVS KSKANLEKIC RTLEDQLSEA 1270 1280 1290 1300 1310 1320 RGKNEEMQRS LSELTTQKSR LQTEAGELSR QLEEKESIVS QLSRSKQAFT QQIEELKRQL 1330 1340 1350 1360 1370 1380 EEENKAKNAL AHALQSSRHD CDLLREQYEE EQEGKAELQR ALSKANSEVA QWRTKYETDA 1390 1400 1410 1420 1430 1440 IQRTEELEEA KKKLAQRLQD SEEQVEAVNA KCASLEKTKQ RLQGEVEDLM VDVERANSLA 1450 1460 1470 1480 1490 1500 AALDKKQRNF DKVLAEWKTK CEESQAELEA ALKESRSLST ELFKLKNAYE EALDQLETVK 1510 1520 1530 1540 1550 1560 RENKNLEQEI ADLTEQIAEN GKSIHELEKS RKQMELEKAD IQMALEEAEA ALEHEEAKIL 1570 1580 1590 1600 1610 1620 RIQLELTQVK SEIDRKIAEK DEEIEQLKRN YQRTVETMQG ALDAEVRSRN EAIRLKKKME 1630 1640 1650 1660 1670 1680 GDLNEIEIQL SHANRQAAET IKHLRSVQGQ LKDTQLHLDD ALRGQEDLKE QLAIVERRAN 1690 1700 1710 1720 1730 1740 LLQAEVEELR ATLEQTERAR KLAEQELLDS NERVQLLHTQ NTSLIHTKKK LETDLTQLQS 1750 1760 1770 1780 1790 1800 EVEDACRDAR NAEEKAKKAI TDAAMMAEEL KKEQDTSAHL ERMKKNLEQT VKDLQHRLDE 1810 1820 1830 1840 1850 1860 AEQLALKGGK KQIQKLETRI RELEFELEGE QKRNTESVKG LRKYERRVKE LTYQSEEDRK 1870 1880 1890 1900 1910 1920 NVLRLQDLVD KLQVKVKSYK RQAEEADEQA NAHLTKFRKA QHELEEAEER ADIAESQVNK 1930 1940 LRAKTRDFTS SRMVVHESEE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 523-1292. Strain: C57BL/6J. Tissue: Head.
[3]	"Genes for skeletal muscle myosin heavy chains are clustered and are not located on the same mouse chromosome as a cardiac myosin heavy chain gene." Weydert A., Daubas P., Lazaridis I., Barton P., Garner I., Leader D.P., Bonhomme F., Catalan J., Simon D., Guenet J.-L., Gros F., Buckingham M.E. Proc. Natl. Acad. Sci. U.S.A. 82:7183-7187(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1747-1905.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL645988, AL596129 Genomic DNA. Translation: CAI25982.2. AL596129, AL645988 Genomic DNA. Translation: CAI24992.2. AK132623 mRNA. Translation: BAE21268.1. M11154 mRNA. Translation: AAA39791.1.
IPI	IPI00380895.
PIR	A24733.
RefSeq	NP_001093105.1. NM_001099635.1.
UniGene	Mm.340090.
3D structure databases
ProteinModelPortal	P13541.
SMR	P13541. Positions 4-964.
ModBase	Search...
PTM databases
PhosphoSite	P13541.
Proteomic databases
PaxDb	P13541.
PRIDE	P13541.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000007301; ENSMUSP00000007301; ENSMUSG00000020908. ENSMUST00000108689; ENSMUSP00000104329; ENSMUSG00000020908. ENSMUST00000165221; ENSMUSP00000131883; ENSMUSG00000020908.
GeneID	17883.
KEGG	mmu:17883.
UCSC	uc007jlx.1. mouse.
Organism-specific databases
CTD	4621.
MGI	MGI:1339709. Myh3.
Phylogenomic databases
eggNOG	COG5022.
GeneTree	ENSGT00680000099790.
HOGENOM	HOG000173959.
HOVERGEN	HBG004704.
InParanoid	P13541.
KO	K10352.
OMA	NGYRGKK.
OrthoDB	EOG43N7BR.
Gene expression databases
ArrayExpress	P13541.
Bgee	P13541.
Genevestigator	P13541.
GermOnline	ENSMUSG00000020908. Mus musculus.
Family and domain databases
Gene3D	4.10.270.10. 1 hit.
InterPro	IPR000048. IQ_motif_EF-hand-BS. IPR027401. Myosin-like_IQ_dom. IPR015650. Myosin_1/23/4/7/8/13/15. IPR001609. Myosin_head_motor_dom. IPR004009. Myosin_N. IPR002928. Myosin_tail. IPR027417. P-loop_NTPase. [Graphical view]
PANTHER	PTHR13140:SF22. PTHR13140:SF22. 1 hit.
Pfam	PF00063. Myosin_head. 1 hit. PF02736. Myosin_N. 1 hit. PF01576. Myosin_tail_1. 1 hit. [Graphical view]
PRINTS	PR00193. MYOSINHEAVY.
SMART	SM00015. IQ. 2 hits. SM00242. MYSc. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit.
PROSITE	PS50096. IQ. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	292685.
SOURCE	Search...

Entry information

Entry name

MYH3_MOUSE

Accession

Primary (citable) accession number: P13541
Secondary accession number(s): Q3V183, Q5SUD4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 9, 2007
Last modified:	May 29, 2013
This is version 96 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program