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P13538

- MYSS_CHICK

UniProt

P13538 - MYSS_CHICK

Protein

Myosin heavy chain, skeletal muscle, adult

Gene
N/A
Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 4 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Muscle contraction. Myosin is a protein that binds to F-actin and has ATPase activity that is activated by F-actin.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi180 – 1878ATPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. motor activity Source: InterPro

    Keywords - Molecular functioni

    Motor protein, Muscle protein, Myosin

    Keywords - Ligandi

    Actin-binding, ATP-binding, Calmodulin-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Myosin heavy chain, skeletal muscle, adult
    OrganismiGallus gallus (Chicken)
    Taxonomic identifieri9031 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
    ProteomesiUP000000539: Unplaced

    Subcellular locationi

    Cytoplasmmyofibril
    Note: Thick filaments of the myofibrils.

    GO - Cellular componenti

    1. myofibril Source: UniProtKB-SubCell
    2. myosin filament Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cytoplasm, Thick filament

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 19391938Myosin heavy chain, skeletal muscle, adultPRO_0000123434Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine
    Modified residuei36 – 361N6-methyllysine
    Modified residuei131 – 1311N6,N6,N6-trimethyllysine
    Modified residuei552 – 5521N6,N6,N6-trimethyllysine
    Modified residuei756 – 7561Pros-methylhistidine

    Keywords - PTMi

    Acetylation, Methylation, Phosphoprotein

    Proteomic databases

    PaxDbiP13538.
    PRIDEiP13538.

    Interactioni

    Subunit structurei

    Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).

    Structurei

    Secondary structure

    1
    1939
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi15 – 184
    Turni22 – 254
    Helixi26 – 294
    Turni35 – 373
    Beta strandi38 – 425
    Beta strandi44 – 5613
    Beta strandi58 – 636
    Beta strandi66 – 683
    Beta strandi71 – 744
    Helixi75 – 773
    Beta strandi86 – 883
    Helixi92 – 943
    Helixi100 – 10910
    Turni110 – 1145
    Beta strandi117 – 1193
    Beta strandi124 – 1274
    Helixi134 – 1363
    Helixi140 – 1434
    Turni144 – 1463
    Helixi156 – 17015
    Beta strandi174 – 1807
    Helixi186 – 20015
    Helixi219 – 23416
    Beta strandi242 – 2454
    Beta strandi247 – 2559
    Beta strandi257 – 2637
    Beta strandi266 – 2694
    Helixi273 – 2764
    Helixi287 – 2926
    Helixi298 – 3047
    Helixi310 – 3123
    Helixi314 – 3163
    Helixi328 – 34114
    Helixi346 – 36217
    Beta strandi367 – 3693
    Beta strandi376 – 3794
    Helixi383 – 3919
    Helixi395 – 4039
    Beta strandi410 – 4123
    Helixi420 – 44930
    Beta strandi458 – 4669
    Beta strandi472 – 4743
    Helixi476 – 50631
    Helixi518 – 52811
    Helixi533 – 5408
    Helixi548 – 55912
    Beta strandi563 – 5675
    Beta strandi579 – 5835
    Beta strandi588 – 5914
    Helixi596 – 6016
    Helixi606 – 6138
    Helixi618 – 6236
    Helixi651 – 66616
    Beta strandi668 – 6769
    Helixi689 – 69911
    Helixi701 – 7088
    Beta strandi715 – 7173
    Helixi718 – 7258
    Helixi726 – 7283
    Turni729 – 7313
    Helixi743 – 7486
    Beta strandi751 – 7533
    Beta strandi756 – 7627
    Beta strandi765 – 7684
    Helixi772 – 82756
    Helixi831 – 8399
    Turni840 – 8423

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1ALMmodel-A1-844[»]
    1M8Qelectron microscopy70.00A/D/G/P5-844[»]
    1MVWelectron microscopy70.00A/D/G/J/M/P5-844[»]
    1O18electron microscopy70.00A/D/G/J/M/P5-844[»]
    1O19electron microscopy70.00A/D/G/J/M/S5-844[»]
    1O1Aelectron microscopy70.00A/D/G/J/M/P5-844[»]
    1O1Belectron microscopy70.00A/D/G/J5-844[»]
    1O1Celectron microscopy70.00A/D/G/J/P5-844[»]
    1O1Delectron microscopy70.00A/D/G/J/M/P5-844[»]
    1O1Eelectron microscopy70.00A/D/G/J/M/P5-844[»]
    1O1Felectron microscopy70.00A/D/G/J5-844[»]
    1O1Gelectron microscopy70.00A/D/G/J/M/P5-844[»]
    2MYSX-ray2.80A2-844[»]
    2W4Aelectron microscopy35.00M5-844[»]
    2W4Gelectron microscopy35.00M5-844[»]
    2W4Helectron microscopy35.00M5-844[»]
    ProteinModelPortaliP13538.
    SMRiP13538. Positions 841-966.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP13538.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini87 – 781695Myosin motorAdd
    BLAST
    Domaini784 – 81330IQPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni658 – 68023Actin-bindingAdd
    BLAST
    Regioni760 – 77415Actin-bindingAdd
    BLAST
    Regioni839 – 8413Hinge

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili842 – 19391098Sequence AnalysisAdd
    BLAST

    Domaini

    The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).

    Sequence similaritiesi

    Contains 1 IQ domain.PROSITE-ProRule annotation
    Contains 1 myosin motor domain.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG5022.
    HOVERGENiHBG004704.
    InParanoidiP13538.
    KOiK10352.

    Family and domain databases

    Gene3Di4.10.270.10. 1 hit.
    InterProiIPR000048. IQ_motif_EF-hand-BS.
    IPR027401. Myosin-like_IQ_dom.
    IPR001609. Myosin_head_motor_dom.
    IPR004009. Myosin_N.
    IPR002928. Myosin_tail.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00063. Myosin_head. 1 hit.
    PF02736. Myosin_N. 1 hit.
    PF01576. Myosin_tail_1. 1 hit.
    [Graphical view]
    PRINTSiPR00193. MYOSINHEAVY.
    SMARTiSM00015. IQ. 1 hit.
    SM00242. MYSc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS50096. IQ. 1 hit.
    PS51456. MYOSIN_MOTOR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P13538-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASPDAEMAA FGEAAPYLRK SEKERIEAQN KPFDAKSSVF VVHPKESFVK     50
    GTIQSKEGGK VTVKTEGGET LTVKEDQVFS MNPPKYDKIE DMAMMTHLHE 100
    PAVLYNLKER YAAWMIYTYS GLFCVTVNPY KWLPVYNPEV VLAYRGKKRQ 150
    EAPPHIFSIS DNAYQFMLTD RENQSILITG ESGAGKTVNT KRVIQYFATI 200
    AASGEKKKEE QSGKMQGTLE DQIISANPLL EAFGNAKTVR NDNSSRFGKF 250
    IRIHFGATGK LASADIETYL LEKSRVTFQL PAERSYHIFY QIMSNKKPEL 300
    IDMLLITTNP YDYHYVSQGE ITVPSIDDQE ELMATDSAID ILGFSADEKT 350
    AIYKLTGAVM HYGNLKFKQK QREEQAEPDG TEVADKAAYL MGLNSAELLK 400
    ALCYPRVKVG NEFVTKGQTV SQVHNSVGAL AKAVYEKMFL WMVIRINQQL 450
    DTKQPRQYFI GVLDIAGFEI FDFNSFEQLC INFTNEKLQQ FFNHHMFVLE 500
    QEEYKKEGIE WEFIDFGMDL AACIELIEKP MGIFSILEEE CMFPKATDTS 550
    FKNKLYDQHL GKSNNFQKPK PAKGKAEAHF SLVHYAGTVD YNISGWLEKN 600
    KDPLNETVIG LYQKSSVKTL ALLFATYGGE AEGGGGKKGG KKKGSSFQTV 650
    SALFRENLNK LMANLRSTHP HFVRCIIPNE TKTPGAMEHE LVLHQLRCNG 700
    VLEGIRICRK GFPSRVLYAD FKQRYRVLNA SAIPEGQFMD SKKASEKLLG 750
    SIDVDHTQYR FGHTKVFFKA GLLGLLEEMR DDKLAEIITR TQARCRGFLM 800
    RVEYRRMVER RESIFCIQYN VRSFMNVKHW PWMKLFFKIK PLLKSAESEK 850
    EMANMKEEFE KTKEELAKSE AKRKELEEKM VVLLQEKNDL QLQVQAEADS 900
    LADAEERCDQ LIKTKIQLEA KIKEVTERAE DEEEINAELT AKKRKLEDEC 950
    SELKKDIDDL ELTLAKVEKE KHATENKVKN LTEEMAVLDE TIAKLTKEKK 1000
    ALQEAHQQTL DDLQVEEDKV NTLTKAKTKL EQQVDDLEGS LEQEKKLRMD 1050
    LERAKRKLEG DLKLAHDSIM DLENDKQQLD EKLKKKDFEI SQIQSKIEDE 1100
    QALGMQLQKK IKELQARIEE LEEEIEAERT SRAKAEKHRA DLSRELEEIS 1150
    ERLEEAGGAT AAQIEMNKKR EAEFQKMRRD LEEATLQHEA TAAALRKKHA 1200
    DSTAELGEQI DNLQRVKQKL EKEKSELKME IDDLASNMES VSKAKANLEK 1250
    MCRTLEDQLS EIKTKEEQNQ RMINDLNTQR ARLQTETGEY SRQAEEKDAL 1300
    ISQLSRGKQG FTQQIEELKR HLEEEIKAKN ALAHALQSAR HDCELLREQY 1350
    EEEQEAKGEL QRALSKANSE VAQWRTKYET DAIQRTEELE EAKKKLAQRL 1400
    QDAEEHVEAV NAKCASLEKT KQRLQNEVED LMVDVERSNA ACAALDKKQK 1450
    NFDKILAEWK QKYEETQTEL EASQKESRSL STELFKMKNA YEESLDHLET 1500
    LKRENKNLQQ EIADLTEQIA EGGKAVHELE KVKKHVEQEK SELQASLEEA 1550
    EASLEHEEGK ILRLQLELNQ IKSEIDRKIA EKDEEIDQLK RNHLRIVESM 1600
    QSTLDAEIRS RNEALRLKKK MEGDLNEMEI QLSHANRMAA EAQKNLRNTQ 1650
    GTLKDTQIHL DDALRTQEDL KEQVAMVERR ANLLQAEVEE LRGALEQTER 1700
    SRKVAEQELL DATERVQLLH TQNTSLINTK KKLETDIVQI QSEMEDTIQE 1750
    ARNAEEKAKK AITDAAMMAE ELKKEQDTSA HLERMKKNMD QTVKDLHVRL 1800
    DEAEQLALKG GKKQLQKLEA RVRELEGEVD SEQKRSAEAV KGVRKYERRV 1850
    KELTYQCEED RKNILRLQDL VDKLQMKVKS YKRQAEEAEE LSNVNLSKFR 1900
    KIQHELEEAE ERADIAESQV NKLRVKSREI HGKKIEEEE 1939
    Length:1,939
    Mass (Da):223,145
    Last modified:January 23, 2007 - v4
    Checksum:iF6DAD73CABD82BFD
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti908 – 9081C → Q AA sequence (PubMed:2610940)Curated
    Sequence conflicti908 – 9081C → Q AA sequence (PubMed:2775482)Curated
    Sequence conflicti981 – 9811L → F in AAB47555. (PubMed:9358064)Curated
    Sequence conflicti1344 – 13441E → D AA sequence (PubMed:1939030)Curated
    Sequence conflicti1546 – 15461S → A AA sequence (PubMed:1939030)Curated
    Sequence conflicti1797 – 17982HV → QL AA sequence (PubMed:1939030)Curated
    Sequence conflicti1831 – 18311S → A AA sequence (PubMed:1939030)Curated
    Sequence conflicti1864 – 18641I → V in AAA48970. (PubMed:3034534)Curated
    Sequence conflicti1930 – 19323IHG → FH in AAA48970. (PubMed:3034534)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U87231 mRNA. Translation: AAB47555.1.
    M16557 Genomic DNA. Translation: AAA48970.1.
    RefSeqiNP_001013415.1. NM_001013397.2.
    UniGeneiGga.51379.

    Genome annotation databases

    GeneIDi427788.
    KEGGigga:427788.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U87231 mRNA. Translation: AAB47555.1 .
    M16557 Genomic DNA. Translation: AAA48970.1 .
    RefSeqi NP_001013415.1. NM_001013397.2.
    UniGenei Gga.51379.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1ALM model - A 1-844 [» ]
    1M8Q electron microscopy 70.00 A/D/G/P 5-844 [» ]
    1MVW electron microscopy 70.00 A/D/G/J/M/P 5-844 [» ]
    1O18 electron microscopy 70.00 A/D/G/J/M/P 5-844 [» ]
    1O19 electron microscopy 70.00 A/D/G/J/M/S 5-844 [» ]
    1O1A electron microscopy 70.00 A/D/G/J/M/P 5-844 [» ]
    1O1B electron microscopy 70.00 A/D/G/J 5-844 [» ]
    1O1C electron microscopy 70.00 A/D/G/J/P 5-844 [» ]
    1O1D electron microscopy 70.00 A/D/G/J/M/P 5-844 [» ]
    1O1E electron microscopy 70.00 A/D/G/J/M/P 5-844 [» ]
    1O1F electron microscopy 70.00 A/D/G/J 5-844 [» ]
    1O1G electron microscopy 70.00 A/D/G/J/M/P 5-844 [» ]
    2MYS X-ray 2.80 A 2-844 [» ]
    2W4A electron microscopy 35.00 M 5-844 [» ]
    2W4G electron microscopy 35.00 M 5-844 [» ]
    2W4H electron microscopy 35.00 M 5-844 [» ]
    ProteinModelPortali P13538.
    SMRi P13538. Positions 841-966.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi P13538.
    PRIDEi P13538.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 427788.
    KEGGi gga:427788.

    Organism-specific databases

    CTDi 427788.

    Phylogenomic databases

    eggNOGi COG5022.
    HOVERGENi HBG004704.
    InParanoidi P13538.
    KOi K10352.

    Miscellaneous databases

    EvolutionaryTracei P13538.
    NextBioi 20828954.

    Family and domain databases

    Gene3Di 4.10.270.10. 1 hit.
    InterProi IPR000048. IQ_motif_EF-hand-BS.
    IPR027401. Myosin-like_IQ_dom.
    IPR001609. Myosin_head_motor_dom.
    IPR004009. Myosin_N.
    IPR002928. Myosin_tail.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00063. Myosin_head. 1 hit.
    PF02736. Myosin_N. 1 hit.
    PF01576. Myosin_tail_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00193. MYOSINHEAVY.
    SMARTi SM00015. IQ. 1 hit.
    SM00242. MYSc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS50096. IQ. 1 hit.
    PS51456. MYOSIN_MOTOR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, nucleotide sequence and characterization of a full-length cDNA encoding the myosin heavy chain from adult chicken pectoralis major muscle."
      Chao T.H., Bandman E.
      Gene 199:265-270(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: White leghorn.
      Tissue: Pectoralis muscle.
    2. "The primary structure of skeletal muscle myosin heavy chain: I. Sequence of the amino-terminal 23 kDa fragment."
      Hayashida M., Maita T., Matsuda G.
      J. Biochem. 110:54-59(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-206.
      Tissue: Pectoralis muscle.
    3. "The primary structure of skeletal muscle myosin heavy chain: II. Sequence of the 50 kDa fragment of subfragment-1."
      Komine Y., Maita T., Matsuda G.
      J. Biochem. 110:60-67(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 207-637.
      Tissue: Pectoralis muscle.
    4. "The primary structure of skeletal muscle myosin heavy chain: III. Sequence of the 22 kDa fragment and the alignment of the 23 kDa, 50 kDa, and 22 kDa fragments."
      Maita T., Miyanishi T., Matsuzono K., Tanioka Y., Matsuda G.
      J. Biochem. 110:68-74(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 638-838.
      Tissue: Pectoralis muscle.
    5. "The primary structure of skeletal muscle myosin heavy chain: IV. Sequence of the rod, and the complete 1,938-residue sequence of the heavy chain."
      Maita T., Yajima E., Nagata S., Miyanishi T., Nakayama S., Matsuda G.
      J. Biochem. 110:75-87(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 839-1939.
      Tissue: Pectoralis muscle.
    6. Cited for: PRELIMINARY PROTEIN SEQUENCE OF 2-809.
    7. "Complete amino-acid sequence of subfragment-2 in adult chicken skeletal muscle myosin."
      Watanabe B.
      Biol. Chem. Hoppe-Seyler 370:1027-1034(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 843-1271.
    8. "Amino-acid sequence of the short subfragment-2 in adult chicken skeletal muscle myosin."
      Watanabe B.
      Biol. Chem. Hoppe-Seyler 370:549-558(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 853-1109.
    9. "Amino-acid sequence of the hinge region in chicken myosin subfragment-2."
      Watanabe B.
      Biol. Chem. Hoppe-Seyler 370:55-61(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1146-1271.
    10. "Genomic clones encoding chicken myosin heavy-chain genes."
      Moriarity D.M., Barringer K.J., Dodgson J.B., Richter H.E., Young R.B.
      DNA 6:91-99(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1858-1939.
    11. Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 1-844.

    Entry informationi

    Entry nameiMYSS_CHICK
    AccessioniPrimary (citable) accession number: P13538
    Secondary accession number(s): O13228
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 134 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3