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P13538 (MYSS_CHICK) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Myosin heavy chain, skeletal muscle, adult
OrganismGallus gallus (Chicken) [Reference proteome]
Taxonomic identifier9031 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus

Protein attributes

Sequence length1939 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Muscle contraction. Myosin is a protein that binds to F-actin and has ATPase activity that is activated by F-actin.

Subunit structure

Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).

Subcellular location

Cytoplasmmyofibril. Note: Thick filaments of the myofibrils.

Domain

The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.

Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).

Sequence similarities

Contains 1 IQ domain.

Contains 1 myosin head-like domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.2 Ref.6
Chain2 – 19391938Myosin heavy chain, skeletal muscle, adult
PRO_0000123434

Regions

Domain2 – 783782Myosin head-like
Domain784 – 81330IQ
Nucleotide binding180 – 1878ATP Potential
Region658 – 68023Actin-binding
Region760 – 77415Actin-binding
Region839 – 8413Hinge
Coiled coil842 – 19391098 Potential

Amino acid modifications

Modified residue21N-acetylalanine
Modified residue361N6-methyllysine
Modified residue1311N6,N6,N6-trimethyllysine
Modified residue5521N6,N6,N6-trimethyllysine
Modified residue7561Pros-methylhistidine

Experimental info

Sequence conflict9081C → Q AA sequence Ref.7
Sequence conflict9081C → Q AA sequence Ref.8
Sequence conflict9811L → F in AAB47555. Ref.1
Sequence conflict13441E → D AA sequence Ref.5
Sequence conflict15461S → A AA sequence Ref.5
Sequence conflict1797 – 17982HV → QL AA sequence Ref.5
Sequence conflict18311S → A AA sequence Ref.5
Sequence conflict18641I → V in AAA48970. Ref.10
Sequence conflict1930 – 19323IHG → FH in AAA48970. Ref.10

Secondary structure

.............................................................................................................................. 1939
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P13538 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: F6DAD73CABD82BFD

FASTA1,939223,145
        10         20         30         40         50         60 
MASPDAEMAA FGEAAPYLRK SEKERIEAQN KPFDAKSSVF VVHPKESFVK GTIQSKEGGK 

        70         80         90        100        110        120 
VTVKTEGGET LTVKEDQVFS MNPPKYDKIE DMAMMTHLHE PAVLYNLKER YAAWMIYTYS 

       130        140        150        160        170        180 
GLFCVTVNPY KWLPVYNPEV VLAYRGKKRQ EAPPHIFSIS DNAYQFMLTD RENQSILITG 

       190        200        210        220        230        240 
ESGAGKTVNT KRVIQYFATI AASGEKKKEE QSGKMQGTLE DQIISANPLL EAFGNAKTVR 

       250        260        270        280        290        300 
NDNSSRFGKF IRIHFGATGK LASADIETYL LEKSRVTFQL PAERSYHIFY QIMSNKKPEL 

       310        320        330        340        350        360 
IDMLLITTNP YDYHYVSQGE ITVPSIDDQE ELMATDSAID ILGFSADEKT AIYKLTGAVM 

       370        380        390        400        410        420 
HYGNLKFKQK QREEQAEPDG TEVADKAAYL MGLNSAELLK ALCYPRVKVG NEFVTKGQTV 

       430        440        450        460        470        480 
SQVHNSVGAL AKAVYEKMFL WMVIRINQQL DTKQPRQYFI GVLDIAGFEI FDFNSFEQLC 

       490        500        510        520        530        540 
INFTNEKLQQ FFNHHMFVLE QEEYKKEGIE WEFIDFGMDL AACIELIEKP MGIFSILEEE 

       550        560        570        580        590        600 
CMFPKATDTS FKNKLYDQHL GKSNNFQKPK PAKGKAEAHF SLVHYAGTVD YNISGWLEKN 

       610        620        630        640        650        660 
KDPLNETVIG LYQKSSVKTL ALLFATYGGE AEGGGGKKGG KKKGSSFQTV SALFRENLNK 

       670        680        690        700        710        720 
LMANLRSTHP HFVRCIIPNE TKTPGAMEHE LVLHQLRCNG VLEGIRICRK GFPSRVLYAD 

       730        740        750        760        770        780 
FKQRYRVLNA SAIPEGQFMD SKKASEKLLG SIDVDHTQYR FGHTKVFFKA GLLGLLEEMR 

       790        800        810        820        830        840 
DDKLAEIITR TQARCRGFLM RVEYRRMVER RESIFCIQYN VRSFMNVKHW PWMKLFFKIK 

       850        860        870        880        890        900 
PLLKSAESEK EMANMKEEFE KTKEELAKSE AKRKELEEKM VVLLQEKNDL QLQVQAEADS 

       910        920        930        940        950        960 
LADAEERCDQ LIKTKIQLEA KIKEVTERAE DEEEINAELT AKKRKLEDEC SELKKDIDDL 

       970        980        990       1000       1010       1020 
ELTLAKVEKE KHATENKVKN LTEEMAVLDE TIAKLTKEKK ALQEAHQQTL DDLQVEEDKV 

      1030       1040       1050       1060       1070       1080 
NTLTKAKTKL EQQVDDLEGS LEQEKKLRMD LERAKRKLEG DLKLAHDSIM DLENDKQQLD 

      1090       1100       1110       1120       1130       1140 
EKLKKKDFEI SQIQSKIEDE QALGMQLQKK IKELQARIEE LEEEIEAERT SRAKAEKHRA 

      1150       1160       1170       1180       1190       1200 
DLSRELEEIS ERLEEAGGAT AAQIEMNKKR EAEFQKMRRD LEEATLQHEA TAAALRKKHA 

      1210       1220       1230       1240       1250       1260 
DSTAELGEQI DNLQRVKQKL EKEKSELKME IDDLASNMES VSKAKANLEK MCRTLEDQLS 

      1270       1280       1290       1300       1310       1320 
EIKTKEEQNQ RMINDLNTQR ARLQTETGEY SRQAEEKDAL ISQLSRGKQG FTQQIEELKR 

      1330       1340       1350       1360       1370       1380 
HLEEEIKAKN ALAHALQSAR HDCELLREQY EEEQEAKGEL QRALSKANSE VAQWRTKYET 

      1390       1400       1410       1420       1430       1440 
DAIQRTEELE EAKKKLAQRL QDAEEHVEAV NAKCASLEKT KQRLQNEVED LMVDVERSNA 

      1450       1460       1470       1480       1490       1500 
ACAALDKKQK NFDKILAEWK QKYEETQTEL EASQKESRSL STELFKMKNA YEESLDHLET 

      1510       1520       1530       1540       1550       1560 
LKRENKNLQQ EIADLTEQIA EGGKAVHELE KVKKHVEQEK SELQASLEEA EASLEHEEGK 

      1570       1580       1590       1600       1610       1620 
ILRLQLELNQ IKSEIDRKIA EKDEEIDQLK RNHLRIVESM QSTLDAEIRS RNEALRLKKK 

      1630       1640       1650       1660       1670       1680 
MEGDLNEMEI QLSHANRMAA EAQKNLRNTQ GTLKDTQIHL DDALRTQEDL KEQVAMVERR 

      1690       1700       1710       1720       1730       1740 
ANLLQAEVEE LRGALEQTER SRKVAEQELL DATERVQLLH TQNTSLINTK KKLETDIVQI 

      1750       1760       1770       1780       1790       1800 
QSEMEDTIQE ARNAEEKAKK AITDAAMMAE ELKKEQDTSA HLERMKKNMD QTVKDLHVRL 

      1810       1820       1830       1840       1850       1860 
DEAEQLALKG GKKQLQKLEA RVRELEGEVD SEQKRSAEAV KGVRKYERRV KELTYQCEED 

      1870       1880       1890       1900       1910       1920 
RKNILRLQDL VDKLQMKVKS YKRQAEEAEE LSNVNLSKFR KIQHELEEAE ERADIAESQV 

      1930 
NKLRVKSREI HGKKIEEEE 

« Hide

References

[1]"Cloning, nucleotide sequence and characterization of a full-length cDNA encoding the myosin heavy chain from adult chicken pectoralis major muscle."
Chao T.H., Bandman E.
Gene 199:265-270(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: White leghorn.
Tissue: Pectoralis muscle.
[2]"The primary structure of skeletal muscle myosin heavy chain: I. Sequence of the amino-terminal 23 kDa fragment."
Hayashida M., Maita T., Matsuda G.
J. Biochem. 110:54-59(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-206.
Tissue: Pectoralis muscle.
[3]"The primary structure of skeletal muscle myosin heavy chain: II. Sequence of the 50 kDa fragment of subfragment-1."
Komine Y., Maita T., Matsuda G.
J. Biochem. 110:60-67(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 207-637.
Tissue: Pectoralis muscle.
[4]"The primary structure of skeletal muscle myosin heavy chain: III. Sequence of the 22 kDa fragment and the alignment of the 23 kDa, 50 kDa, and 22 kDa fragments."
Maita T., Miyanishi T., Matsuzono K., Tanioka Y., Matsuda G.
J. Biochem. 110:68-74(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 638-838.
Tissue: Pectoralis muscle.
[5]"The primary structure of skeletal muscle myosin heavy chain: IV. Sequence of the rod, and the complete 1,938-residue sequence of the heavy chain."
Maita T., Yajima E., Nagata S., Miyanishi T., Nakayama S., Matsuda G.
J. Biochem. 110:75-87(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 839-1939.
Tissue: Pectoralis muscle.
[6]"The primary structure of the myosin head."
Maita T., Hayashida M., Tanioka Y., Komine Y., Matsuda G.
Proc. Natl. Acad. Sci. U.S.A. 84:416-420(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: PRELIMINARY PROTEIN SEQUENCE OF 2-809.
[7]"Complete amino-acid sequence of subfragment-2 in adult chicken skeletal muscle myosin."
Watanabe B.
Biol. Chem. Hoppe-Seyler 370:1027-1034(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 843-1271.
[8]"Amino-acid sequence of the short subfragment-2 in adult chicken skeletal muscle myosin."
Watanabe B.
Biol. Chem. Hoppe-Seyler 370:549-558(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 853-1109.
[9]"Amino-acid sequence of the hinge region in chicken myosin subfragment-2."
Watanabe B.
Biol. Chem. Hoppe-Seyler 370:55-61(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1146-1271.
[10]"Genomic clones encoding chicken myosin heavy-chain genes."
Moriarity D.M., Barringer K.J., Dodgson J.B., Richter H.E., Young R.B.
DNA 6:91-99(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1858-1939.
[11]"Three-dimensional structure of myosin subfragment-1: a molecular motor."
Rayment I., Rypniewski W.R., Schmidt-Base K., Smith R., Tomchick D.R., Benning M.M., Winkelmann D.A., Wesenberg G., Holden H.M.
Science 261:50-58(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 1-844.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U87231 mRNA. Translation: AAB47555.1.
M16557 Genomic DNA. Translation: AAA48970.1.
RefSeqNP_001013415.1. NM_001013397.2.
UniGeneGga.51379.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1ALMmodel-A1-844[»]
1M8Qelectron microscopy70.00A/D/G/P5-844[»]
1MVWelectron microscopy70.00A/D/G/J/M/P5-843[»]
1O18electron microscopy70.00A/D/G/J/M/P5-843[»]
1O19electron microscopy70.00A/D/G/J/M/S5-843[»]
1O1Aelectron microscopy70.00A/D/G/J/M/P5-843[»]
1O1Belectron microscopy70.00A/D/G/J5-843[»]
1O1Celectron microscopy70.00A/D/G/J/P5-843[»]
1O1Delectron microscopy70.00A/D/G/J/M/P5-843[»]
1O1Eelectron microscopy70.00A/D/G/J/M/P5-843[»]
1O1Felectron microscopy70.00A/D/G/J5-843[»]
1O1Gelectron microscopy70.00A/D/G/J/M/P5-843[»]
2MYSX-ray2.80A2-844[»]
2W4Aelectron microscopy35.00M5-844[»]
2W4Gelectron microscopy35.00M5-844[»]
2W4Helectron microscopy35.00M5-844[»]
ProteinModelPortalP13538.
SMRP13538. Positions 841-966.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbP13538.
PRIDEP13538.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID427788.
KEGGgga:427788.

Organism-specific databases

CTD427788.

Phylogenomic databases

eggNOGCOG5022.
HOVERGENHBG004704.
InParanoidP13538.
KOK10352.

Family and domain databases

Gene3D4.10.270.10. 1 hit.
InterProIPR000048. IQ_motif_EF-hand-BS.
IPR027401. Myosin-like_IQ_dom.
IPR015650. Myosin_1/23/4/6/7/8/13/15.
IPR001609. Myosin_head_motor_dom.
IPR004009. Myosin_N.
IPR002928. Myosin_tail.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERPTHR13140:SF22. PTHR13140:SF22. 1 hit.
PfamPF00063. Myosin_head. 1 hit.
PF02736. Myosin_N. 1 hit.
PF01576. Myosin_tail_1. 1 hit.
[Graphical view]
PRINTSPR00193. MYOSINHEAVY.
SMARTSM00015. IQ. 1 hit.
SM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS50096. IQ. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP13538.
NextBio20828954.

Entry information

Entry nameMYSS_CHICK
AccessionPrimary (citable) accession number: P13538
Secondary accession number(s): O13228
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 130 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references